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P63212

- GBG2_BOVIN

UniProt

P63212 - GBG2_BOVIN

Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Gene

GNG2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. G-protein beta-subunit binding Source: UniProt
    2. GTPase activity Source: MGI
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. adenylate cyclase-activating dopamine receptor signaling pathway Source: BHF-UCL
    2. cell proliferation Source: Ensembl
    3. cellular response to catecholamine stimulus Source: BHF-UCL
    4. cellular response to prostaglandin E stimulus Source: BHF-UCL
    5. G-protein coupled receptor signaling pathway Source: MGI
    6. GTP catabolic process Source: GOC

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_202137. Regulation of water balance by renal Aquaporins.
    REACT_202218. G beta:gamma signalling through PI3Kgamma.
    REACT_205384. G alpha (12/13) signalling events.
    REACT_206547. G alpha (s) signalling events.
    REACT_208538. Activation of G protein gated Potassium channels.
    REACT_209033. G alpha (z) signalling events.
    REACT_209521. G alpha (q) signalling events.
    REACT_209727. Ca2+ pathway.
    REACT_210152. Thromboxane signalling through TP receptor.
    REACT_210568. G alpha (i) signalling events.
    REACT_211545. ADP signalling through P2Y purinoceptor 1.
    REACT_215912. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_217228. Presynaptic function of Kainate receptors.
    REACT_217846. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_218219. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_219437. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_219794. ADP signalling through P2Y purinoceptor 12.
    REACT_220607. Glucagon-type ligand receptors.
    REACT_221707. Glucagon signaling in metabolic regulation.
    REACT_222419. G-protein activation.
    REACT_224596. G beta:gamma signalling through PLC beta.
    REACT_227415. Prostacyclin signalling through prostacyclin receptor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Alternative name(s):
    G gamma-I
    Gene namesi
    Name:GNG2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 10

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. heterotrimeric G-protein complex Source: BHF-UCL
    2. membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 6867Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2PRO_0000012609Add
    BLAST
    Propeptidei69 – 713Removed in mature formPRO_0000012610

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei68 – 681Cysteine methyl ester
    Lipidationi68 – 681S-geranylgeranyl cysteine2 Publications

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PRIDEiP63212.

    Expressioni

    Tissue specificityi

    Adrenal gland and brain.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-586N.
    IntActiP63212. 1 interaction.

    Structurei

    Secondary structure

    1
    71
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 2315
    Helixi30 – 4314
    Helixi45 – 473
    Turni49 – 513
    Helixi56 – 583
    Turni60 – 634

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GG2X-ray2.40G1-71[»]
    1GP2X-ray2.30G1-71[»]
    1OMWX-ray2.50G1-68[»]
    1XHMX-ray2.70B1-71[»]
    2BCJX-ray3.06G1-71[»]
    3AH8X-ray2.90G1-71[»]
    3CIKX-ray2.75G1-68[»]
    3KRWX-ray2.90G1-68[»]
    3KRXX-ray3.10G1-68[»]
    3PSCX-ray2.67G1-68[»]
    3PVUX-ray2.48G1-68[»]
    3PVWX-ray2.49G1-68[»]
    3SN6X-ray3.20G1-68[»]
    3UZSX-ray4.52G1-67[»]
    3V5WX-ray2.07G1-71[»]
    4MK0X-ray2.40G6-64[»]
    ProteinModelPortaliP63212.
    SMRiP63212. Positions 8-64.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63212.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G protein gamma family.Curated

    Phylogenomic databases

    eggNOGiNOG298292.
    GeneTreeiENSGT00740000115378.
    HOVERGENiHBG014983.
    InParanoidiP63212.
    KOiK07826.
    OMAiSCTEARE.
    OrthoDBiEOG7H1JP7.
    TreeFamiTF319909.

    Family and domain databases

    Gene3Di4.10.260.10. 1 hit.
    InterProiIPR015898. G-protein_gamma-like_dom.
    IPR001770. Gprotein-gamma.
    [Graphical view]
    PANTHERiPTHR13809. PTHR13809. 1 hit.
    PfamiPF00631. G-gamma. 1 hit.
    [Graphical view]
    PRINTSiPR00321. GPROTEING.
    SMARTiSM00224. GGL. 1 hit.
    [Graphical view]
    SUPFAMiSSF48670. SSF48670. 1 hit.
    PROSITEiPS50058. G_PROTEIN_GAMMA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63212-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASNNTASIA QARKLVEQLK MEANIDRIKV SKAAADLMAY CEAHAKEDPL   50
    LTPVPASENP FREKKFFCAI L 71
    Length:71
    Mass (Da):7,850
    Last modified:January 23, 2007 - v2
    Checksum:iEDB74E4135E7A37A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05071 mRNA. Translation: AAA30541.1.
    M37183 mRNA. Translation: AAA30555.1.
    BC112789 mRNA. Translation: AAI12790.1.
    PIRiB34228. RGBOG2.
    RefSeqiNP_776497.1. NM_174072.4.
    XP_005211711.1. XM_005211654.1.
    UniGeneiBt.4586.
    Bt.90717.

    Genome annotation databases

    EnsembliENSBTAT00000003959; ENSBTAP00000003959; ENSBTAG00000003043.
    GeneIDi281203.
    KEGGibta:281203.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05071 mRNA. Translation: AAA30541.1 .
    M37183 mRNA. Translation: AAA30555.1 .
    BC112789 mRNA. Translation: AAI12790.1 .
    PIRi B34228. RGBOG2.
    RefSeqi NP_776497.1. NM_174072.4.
    XP_005211711.1. XM_005211654.1.
    UniGenei Bt.4586.
    Bt.90717.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GG2 X-ray 2.40 G 1-71 [» ]
    1GP2 X-ray 2.30 G 1-71 [» ]
    1OMW X-ray 2.50 G 1-68 [» ]
    1XHM X-ray 2.70 B 1-71 [» ]
    2BCJ X-ray 3.06 G 1-71 [» ]
    3AH8 X-ray 2.90 G 1-71 [» ]
    3CIK X-ray 2.75 G 1-68 [» ]
    3KRW X-ray 2.90 G 1-68 [» ]
    3KRX X-ray 3.10 G 1-68 [» ]
    3PSC X-ray 2.67 G 1-68 [» ]
    3PVU X-ray 2.48 G 1-68 [» ]
    3PVW X-ray 2.49 G 1-68 [» ]
    3SN6 X-ray 3.20 G 1-68 [» ]
    3UZS X-ray 4.52 G 1-67 [» ]
    3V5W X-ray 2.07 G 1-71 [» ]
    4MK0 X-ray 2.40 G 6-64 [» ]
    ProteinModelPortali P63212.
    SMRi P63212. Positions 8-64.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-586N.
    IntActi P63212. 1 interaction.

    Proteomic databases

    PRIDEi P63212.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000003959 ; ENSBTAP00000003959 ; ENSBTAG00000003043 .
    GeneIDi 281203.
    KEGGi bta:281203.

    Organism-specific databases

    CTDi 54331.

    Phylogenomic databases

    eggNOGi NOG298292.
    GeneTreei ENSGT00740000115378.
    HOVERGENi HBG014983.
    InParanoidi P63212.
    KOi K07826.
    OMAi SCTEARE.
    OrthoDBi EOG7H1JP7.
    TreeFami TF319909.

    Enzyme and pathway databases

    Reactomei REACT_202137. Regulation of water balance by renal Aquaporins.
    REACT_202218. G beta:gamma signalling through PI3Kgamma.
    REACT_205384. G alpha (12/13) signalling events.
    REACT_206547. G alpha (s) signalling events.
    REACT_208538. Activation of G protein gated Potassium channels.
    REACT_209033. G alpha (z) signalling events.
    REACT_209521. G alpha (q) signalling events.
    REACT_209727. Ca2+ pathway.
    REACT_210152. Thromboxane signalling through TP receptor.
    REACT_210568. G alpha (i) signalling events.
    REACT_211545. ADP signalling through P2Y purinoceptor 1.
    REACT_215912. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_217228. Presynaptic function of Kainate receptors.
    REACT_217846. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_218219. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_219437. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_219794. ADP signalling through P2Y purinoceptor 12.
    REACT_220607. Glucagon-type ligand receptors.
    REACT_221707. Glucagon signaling in metabolic regulation.
    REACT_222419. G-protein activation.
    REACT_224596. G beta:gamma signalling through PLC beta.
    REACT_227415. Prostacyclin signalling through prostacyclin receptor.

    Miscellaneous databases

    EvolutionaryTracei P63212.
    NextBioi 20805259.

    Family and domain databases

    Gene3Di 4.10.260.10. 1 hit.
    InterProi IPR015898. G-protein_gamma-like_dom.
    IPR001770. Gprotein-gamma.
    [Graphical view ]
    PANTHERi PTHR13809. PTHR13809. 1 hit.
    Pfami PF00631. G-gamma. 1 hit.
    [Graphical view ]
    PRINTSi PR00321. GPROTEING.
    SMARTi SM00224. GGL. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48670. SSF48670. 1 hit.
    PROSITEi PS50058. G_PROTEIN_GAMMA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Existence of two gamma subunits of the G proteins in brain."
      Robishaw J.D., Kalman V.K., Moomaw C.R., Slaughter C.A.
      J. Biol. Chem. 264:15758-15761(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 14-62.
      Tissue: Adrenal gland and Brain.
    2. "A G protein gamma subunit shares homology with ras proteins."
      Gautam N., Baetscher M., Aebersold R., Simon M.I.
      Science 244:971-974(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Kidney.
    4. "Determination of the complete covalent structure of the gamma 2 subunit of bovine brain G proteins by mass spectrometry."
      Wilcox M.D., Schey K.L., Busman M., Hildebrandt J.D.
      Biochem. Biophys. Res. Commun. 212:367-374(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-71, ACETYLATION AT ALA-2, ISOPRENYLATION AT CYS-68.
      Tissue: Brain.
    5. "Identification of a novel gamma-subunit from bovine brain GTP binding regulatory proteins (Gi/o)."
      Sohma H., Hashimoto H., Hiraike N., Ohguro H., Akino T.
      Biochem. Biophys. Res. Commun. 190:849-856(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-64.
      Tissue: Brain.
    6. "Gamma-subunits of G proteins, but not their alpha- or beta-subunits, are polyisoprenylated. Studies on post-translational modifications using in vitro translation with rabbit reticulocyte lysates."
      Sanford J., Codina J., Birnbaumer L.
      J. Biol. Chem. 266:9570-9579(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-68.
    7. "The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2."
      Wall M.A., Coleman D.E., Lee E., Iniguez-Lluhi J.A., Posner B.A., Gilman A.G., Sprang S.R.
      Cell 83:1047-1058(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF HETEROTRIMER.

    Entry informationi

    Entry nameiGBG2_BOVIN
    AccessioniPrimary (citable) accession number: P63212
    Secondary accession number(s): P16874
    , Q2KI31, Q61013, Q9TS47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3