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Protein

Transcriptional regulator GadE

Gene

gadE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulates the expression of several genes involved in acid resistance. Required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. Binds directly to the 20 bp GAD box found in the control regions of both loci.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi133 – 15220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11544-MONOMER.
ECOL316407:JW3480-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator GadE
Gene namesi
Name:gadE
Synonyms:yhiE, yhiT
Ordered Locus Names:b3512, JW3480
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11544. gadE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Transcriptional regulator GadEPRO_0000184155Add
BLAST

Proteomic databases

PaxDbiP63204.
PRIDEiP63204.

Expressioni

Inductioni

By acidic conditions. Could be induced by EvgA via the induction of YdeO.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262519. 8 interactions.
DIPiDIP-48091N.
IntActiP63204. 2 interactions.
STRINGi511145.b3512.

Structurei

3D structure databases

ProteinModelPortaliP63204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 17466HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106WJ0. Bacteria.
ENOG410YREC. LUCA.
HOGENOMiHOG000125848.
OMAiIANWISL.
OrthoDBiEOG6M9DVS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.20.20.150. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFLMTKDSF LLQGFWQLKD NHEMIKINSL SEIKKVGNKP FKVIIDTYHN
60 70 80 90 100
HILDEEAIKF LEKLDAERII VLAPYHISKL KAKAPIYFVS RKESIKNLLE
110 120 130 140 150
ITYGKHLPHK NSQLCFSHNQ FKIMQLILKN KNESNITSTL NISQQTLKIQ
160 170
KFNIMYKLKL RRMSDIVTLG ITSYF
Length:175
Mass (Da):20,599
Last modified:September 27, 2004 - v1
Checksum:i5CCC69A0DA53B293
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18488.1.
U00096 Genomic DNA. Translation: AAC76537.1.
AP009048 Genomic DNA. Translation: BAE77782.1.
D11389 Genomic DNA. No translation available.
PIRiS47732.
RefSeqiNP_417969.1. NC_000913.3.
WP_000576690.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76537; AAC76537; b3512.
BAE77782; BAE77782; BAE77782.
GeneIDi948023.
KEGGiecj:JW3480.
eco:b3512.
PATRICi32122482. VBIEscCol129921_3619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18488.1.
U00096 Genomic DNA. Translation: AAC76537.1.
AP009048 Genomic DNA. Translation: BAE77782.1.
D11389 Genomic DNA. No translation available.
PIRiS47732.
RefSeqiNP_417969.1. NC_000913.3.
WP_000576690.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP63204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262519. 8 interactions.
DIPiDIP-48091N.
IntActiP63204. 2 interactions.
STRINGi511145.b3512.

Proteomic databases

PaxDbiP63204.
PRIDEiP63204.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76537; AAC76537; b3512.
BAE77782; BAE77782; BAE77782.
GeneIDi948023.
KEGGiecj:JW3480.
eco:b3512.
PATRICi32122482. VBIEscCol129921_3619.

Organism-specific databases

EchoBASEiEB1506.
EcoGeneiEG11544. gadE.

Phylogenomic databases

eggNOGiENOG4106WJ0. Bacteria.
ENOG410YREC. LUCA.
HOGENOMiHOG000125848.
OMAiIANWISL.
OrthoDBiEOG6M9DVS.

Enzyme and pathway databases

BioCyciEcoCyc:EG11544-MONOMER.
ECOL316407:JW3480-MONOMER.

Miscellaneous databases

PROiP63204.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.20.20.150. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Physical map location of a set of Escherichia coli genes (hde) whose expression is affected by the nucleoid protein H-NS."
    Yoshida T., Ueguchi C., Mizuno T.
    J. Bacteriol. 175:7747-7748(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
    Strain: K12.
  5. "Escherichia coli gene expression responsive to levels of the response regulator EvgA."
    Masuda N., Church G.M.
    J. Bacteriol. 184:6225-6234(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Regulatory network of acid resistance genes in Escherichia coli."
    Masuda N., Church G.M.
    Mol. Microbiol. 48:699-712(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12."
    Ma Z., Gong S., Richard H., Tucker D.L., Conway T., Foster J.W.
    Mol. Microbiol. 49:1309-1320(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.
  8. "GadE (YhiE): a novel activator involved in the response to acid environment in Escherichia coli."
    Hommais F., Krin E., Coppee J.-Y., Lacroix C., Yeramian E., Danchin A., Bertin P.
    Microbiology 150:61-72(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: FB8.

Entry informationi

Entry nameiGADE_ECOLI
AccessioniPrimary (citable) accession number: P63204
Secondary accession number(s): P29688, Q2M7H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: January 20, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.