Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

HTH-type transcriptional regulator GadW

Gene

gadW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of GadW to the gadA and gadBC promoters.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi155 – 174H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • core promoter proximal region sequence-specific DNA binding Source: GO_Central
  • DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12242-MONOMER.
ECOL316407:JW3483-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator GadW
Gene namesi
Name:gadW
Synonyms:yhiW
Ordered Locus Names:b3515, JW3483
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12242. gadW.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001945141 – 242HTH-type transcriptional regulator GadWAdd BLAST242

Proteomic databases

PaxDbiP63201.
PRIDEiP63201.

Expressioni

Inductioni

Expression can be repressed by GadX, depending on the conditions.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262522. 8 interactors.
IntActiP63201. 6 interactors.
STRINGi511145.b3515.

Structurei

3D structure databases

ProteinModelPortaliP63201.
SMRiP63201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000120708.
InParanoidiP63201.
PhylomeDBiP63201.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHVCSVILI RRSFDIYHEQ QKISLHNESI LLLEKNLADD FAFCSPDTRR
60 70 80 90 100
LDIDELTVCH YLQNIRQLPR NLGLHSKDRL LINQSPPMPL VTAIFDSFNE
110 120 130 140 150
SGVNSPILSN MLYLSCLSMF SHKKELIPLL FNSISTVSGK VERLISFDIA
160 170 180 190 200
KRWYLRDIAE RMYTSESLIK KKLQDENTCF SKILLASRMS MARRLLELRQ
210 220 230 240
IPLHTIAEKC GYSSTSYFIN TFRQYYGVTP HQFAQHSPGT FS
Length:242
Mass (Da):28,028
Last modified:September 27, 2004 - v1
Checksum:i40C11B74DA2F8DA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18491.1.
U00096 Genomic DNA. Translation: AAC76540.1.
AP009048 Genomic DNA. Translation: BAE77779.1.
PIRiS47735.
RefSeqiNP_417972.1. NC_000913.3.
WP_000149999.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76540; AAC76540; b3515.
BAE77779; BAE77779; BAE77779.
GeneIDi948029.
KEGGiecj:JW3483.
eco:b3515.
PATRICi32122490. VBIEscCol129921_3623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18491.1.
U00096 Genomic DNA. Translation: AAC76540.1.
AP009048 Genomic DNA. Translation: BAE77779.1.
PIRiS47735.
RefSeqiNP_417972.1. NC_000913.3.
WP_000149999.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP63201.
SMRiP63201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262522. 8 interactors.
IntActiP63201. 6 interactors.
STRINGi511145.b3515.

Proteomic databases

PaxDbiP63201.
PRIDEiP63201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76540; AAC76540; b3515.
BAE77779; BAE77779; BAE77779.
GeneIDi948029.
KEGGiecj:JW3483.
eco:b3515.
PATRICi32122490. VBIEscCol129921_3623.

Organism-specific databases

EchoBASEiEB2153.
EcoGeneiEG12242. gadW.

Phylogenomic databases

HOGENOMiHOG000120708.
InParanoidiP63201.
PhylomeDBiP63201.

Enzyme and pathway databases

BioCyciEcoCyc:EG12242-MONOMER.
ECOL316407:JW3483-MONOMER.

Miscellaneous databases

PROiP63201.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGADW_ECOLI
AccessioniPrimary (citable) accession number: P63201
Secondary accession number(s): P37638, Q2M7H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GadX and GadW are part of a complex system required for acid resistance via the regulation of gadA and gadBC. Many of the described circuits use mutants missing one of the network components. In the wild-type situation, however, many of the scenarios are not easily observed.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.