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Protein

Low affinity potassium transport system protein kup

Gene

kup

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Responsible for the low-affinity transport of potassium into the cell, with the probable concomitant uptake of protons (symport system). Can also transport cesium.3 Publications

GO - Molecular functioni

  • potassium ion transmembrane transporter activity Source: EcoCyc
  • symporter activity Source: UniProtKB-HAMAP

GO - Biological processi

  • potassium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Symport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:KUP-MONOMER.
ECOL316407:JW5609-MONOMER.
MetaCyc:KUP-MONOMER.

Protein family/group databases

TCDBi2.A.72.1.1. the k(+) uptake permease (kup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low affinity potassium transport system protein kup
Alternative name(s):
Kup system potassium uptake protein
Gene namesi
Name:kup
Synonyms:trkD
Ordered Locus Names:b3747, JW5609
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11541. kup.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 48PeriplasmicSequence analysisAdd BLAST19
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 102CytoplasmicSequence analysisAdd BLAST33
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 136PeriplasmicSequence analysisAdd BLAST13
Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
Topological domaini158 – 164CytoplasmicSequence analysis7
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 212PeriplasmicSequence analysisAdd BLAST27
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 246CytoplasmicSequence analysisAdd BLAST13
Transmembranei247 – 267HelicalSequence analysisAdd BLAST21
Topological domaini268 – 275PeriplasmicSequence analysis8
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 336CytoplasmicSequence analysisAdd BLAST40
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 362PeriplasmicSequence analysis5
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 395CytoplasmicSequence analysisAdd BLAST12
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Topological domaini417 – 418PeriplasmicSequence analysis2
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21
Topological domaini440 – 622CytoplasmicSequence analysisAdd BLAST183

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002090111 – 622Low affinity potassium transport system protein kupAdd BLAST622

Proteomic databases

PaxDbiP63183.
PRIDEiP63183.

Interactioni

Protein-protein interaction databases

BioGridi4263467. 7 interactors.
STRINGi511145.b3747.

Structurei

3D structure databases

ProteinModelPortaliP63183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DFH. Bacteria.
COG3158. LUCA.
HOGENOMiHOG000218033.
InParanoidiP63183.
KOiK03549.
OMAiISYVMRA.
PhylomeDBiP63183.

Family and domain databases

HAMAPiMF_01522. Kup. 1 hit.
InterProiIPR003855. K+_transporter.
IPR023051. K+_uptake_low_affin.
[Graphical view]
PfamiPF02705. K_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00794. kup. 1 hit.

Sequencei

Sequence statusi: Complete.

P63183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDNKQSLP AITLAAIGVV YGDIGTSPLY TLRECLSGQF GFGVERDAVF
60 70 80 90 100
GFLSLIFWLL IFVVSIKYLT FVMRADNAGE GGILTLMSLA GRNTSARTTS
110 120 130 140 150
MLVIMGLIGG SFFYGEVVIT PAISVMSAIE GLEIVAPQLD TWIVPLSIIV
160 170 180 190 200
LTLLFMIQKH GTAMVGKLFA PIMLTWFLIL AGLGLRSIIA NPEVLHALNP
210 220 230 240 250
MWAVHFFLEY KTVSFIALGA VVLSITGVEA LYADMGHFGK FPIRLAWFTV
260 270 280 290 300
VLPSLTLNYF GQGALLLKNP EAIKNPFFLL APDWALIPLL IIAALATVIA
310 320 330 340 350
SQAVISGVFS LTRQAVRLGY LSPMRIIHTS EMESGQIYIP FVNWMLYVAV
360 370 380 390 400
VIVIVSFEHS SNLAAAYGIA VTGTMVLTSI LSTTVARQNW HWNKYFVALI
410 420 430 440 450
LIAFLCVDIP LFTANLDKLL SGGWLPLSLG TVMFIVMTTW KSERFRLLRR
460 470 480 490 500
MHEHGNSLEA MIASLEKSPP VRVPGTAVYM SRAINVIPFA LMHNLKHNKV
510 520 530 540 550
LHERVILLTL RTEDAPYVHN VRRVQIEQLS PTFWRVVASY GWRETPNVEE
560 570 580 590 600
VFHRCGLEGL SCRMMETSFF MSHESLILGK RPWYLRLRGK LYLLLQRNAL
610 620
RAPDQFEIPP NRVIELGTQV EI
Length:622
Mass (Da):69,294
Last modified:September 27, 2004 - v1
Checksum:iE556791ABF9B8D25
GO

Sequence cautioni

The sequence AAA62100 differs from that shown. Reason: Frameshift at positions 506, 542 and 579.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68551 Genomic DNA. Translation: CAA48555.1.
L10328 Genomic DNA. Translation: AAA62100.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48204.1.
AP009048 Genomic DNA. Translation: BAE77541.1.
PIRiA49345.
RefSeqiWP_000102319.1. NZ_LN832404.1.
YP_026244.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48204; AAT48204; b3747.
BAE77541; BAE77541; BAE77541.
GeneIDi948255.
KEGGiecj:JW5609.
eco:b3747.
PATRICi32122993. VBIEscCol129921_3872.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68551 Genomic DNA. Translation: CAA48555.1.
L10328 Genomic DNA. Translation: AAA62100.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48204.1.
AP009048 Genomic DNA. Translation: BAE77541.1.
PIRiA49345.
RefSeqiWP_000102319.1. NZ_LN832404.1.
YP_026244.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP63183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263467. 7 interactors.
STRINGi511145.b3747.

Protein family/group databases

TCDBi2.A.72.1.1. the k(+) uptake permease (kup) family.

Proteomic databases

PaxDbiP63183.
PRIDEiP63183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48204; AAT48204; b3747.
BAE77541; BAE77541; BAE77541.
GeneIDi948255.
KEGGiecj:JW5609.
eco:b3747.
PATRICi32122993. VBIEscCol129921_3872.

Organism-specific databases

EchoBASEiEB1503.
EcoGeneiEG11541. kup.

Phylogenomic databases

eggNOGiENOG4105DFH. Bacteria.
COG3158. LUCA.
HOGENOMiHOG000218033.
InParanoidiP63183.
KOiK03549.
OMAiISYVMRA.
PhylomeDBiP63183.

Enzyme and pathway databases

BioCyciEcoCyc:KUP-MONOMER.
ECOL316407:JW5609-MONOMER.
MetaCyc:KUP-MONOMER.

Miscellaneous databases

PROiP63183.

Family and domain databases

HAMAPiMF_01522. Kup. 1 hit.
InterProiIPR003855. K+_transporter.
IPR023051. K+_uptake_low_affin.
[Graphical view]
PfamiPF02705. K_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00794. kup. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKUP_ECOLI
AccessioniPrimary (citable) accession number: P63183
Secondary accession number(s): P30016, P76748, Q2M865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Kup is probably the major potassium uptake system upon hyper-osmotic stress at a low pH.
Uptake is inhibited by protonophores such as CCCP.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.