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P63177

- RLMB_ECOLI

UniProt

P63177 - RLMB_ECOLI

Protein

23S rRNA (guanosine-2'-O-)-methyltransferase RlmB

Gene

rlmB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (27 Sep 2004)
      Previous versions | rss
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    Functioni

    Specifically methylates the ribose of guanosine 2251 in 23S rRNA.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei196 – 1961S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei216 – 2161S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei225 – 2251S-adenosyl-L-methionine; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. rRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. enzyme-directed rRNA 2'-O-methylation Source: EcoCyc

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:G7845-MONOMER.
    ECOL316407:JW4138-MONOMER.
    MetaCyc:G7845-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (EC:2.1.1.185)
    Alternative name(s):
    23S rRNA (guanosine2251 2'-O)-methyltransferase
    23S rRNA Gm2251 2'-O-methyltransferase
    Gene namesi
    Name:rlmB
    Synonyms:yjfH
    Ordered Locus Names:b4180, JW4138
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12483. rlmB.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 24324323S rRNA (guanosine-2'-O-)-methyltransferase RlmBPRO_0000159785Add
    BLAST

    Proteomic databases

    PaxDbiP63177.
    PRIDEiP63177.

    Expressioni

    Gene expression databases

    GenevestigatoriP63177.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-47866N.
    IntActiP63177. 19 interactions.
    STRINGi511145.b4180.

    Structurei

    Secondary structure

    1
    243
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Helixi8 – 169
    Helixi19 – 213
    Beta strandi22 – 309
    Turni34 – 363
    Helixi37 – 4610
    Beta strandi49 – 535
    Helixi56 – 605
    Turni61 – 633
    Beta strandi70 – 745
    Helixi82 – 843
    Helixi85 – 906
    Beta strandi96 – 1027
    Helixi106 – 11914
    Beta strandi122 – 13110
    Helixi136 – 1427
    Helixi145 – 1484
    Beta strandi151 – 1555
    Helixi157 – 16610
    Beta strandi170 – 1745
    Beta strandi179 – 1813
    Helixi182 – 1843
    Beta strandi189 – 1979
    Turni198 – 2003
    Helixi204 – 2085
    Beta strandi211 – 2155
    Beta strandi219 – 2224
    Helixi227 – 24115

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
    ProteinModelPortaliP63177.
    SMRiP63177. Positions 1-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63177.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0566.
    HOGENOMiHOG000218798.
    KOiK03218.
    OMAiIFGFHAV.
    OrthoDBiEOG6GBMDM.
    PhylomeDBiP63177.

    Family and domain databases

    Gene3Di3.30.1330.30. 1 hit.
    3.40.1280.10. 1 hit.
    HAMAPiMF_01887. 23SrRNA_methyltr_B.
    InterProiIPR024915. 23S_rRNA_MeTrfase_RlmB.
    IPR029028. Alpha/beta_knot_MTases.
    IPR029064. L30e-like.
    IPR004441. rRNA_MeTrfase_TrmH.
    IPR001537. SpoU_MeTrfase.
    IPR013123. SpoU_subst-bd.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    PF08032. SpoU_sub_bind. 1 hit.
    [Graphical view]
    SMARTiSM00967. SpoU_sub_bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF55315. SSF55315. 1 hit.
    SSF75217. SSF75217. 1 hit.
    TIGRFAMsiTIGR00186. rRNA_methyl_3. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P63177-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI    50
    QLANRQYLDE KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL 100
    DGVTDPHNLG ACLRSADAAG VHAVIVPKDR SAQLNATAKK VACGAAESVP 150
    LIRVTNLART MRMLQEENIW IVGTAGEADH TLYQSKMTGR LALVMGAEGE 200
    GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR QRS 243
    Length:243
    Mass (Da):26,557
    Last modified:September 27, 2004 - v1
    Checksum:i6D6C4170580341D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14003 Genomic DNA. Translation: AAA97076.1.
    U00096 Genomic DNA. Translation: AAC77137.1.
    AP009048 Genomic DNA. Translation: BAE78181.1.
    PIRiS56405.
    RefSeqiNP_418601.1. NC_000913.3.
    YP_492322.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77137; AAC77137; b4180.
    BAE78181; BAE78181; BAE78181.
    GeneIDi12932817.
    948694.
    KEGGiecj:Y75_p4066.
    eco:b4180.
    PATRICi32123935. VBIEscCol129921_4312.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14003 Genomic DNA. Translation: AAA97076.1 .
    U00096 Genomic DNA. Translation: AAC77137.1 .
    AP009048 Genomic DNA. Translation: BAE78181.1 .
    PIRi S56405.
    RefSeqi NP_418601.1. NC_000913.3.
    YP_492322.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GZ0 X-ray 2.50 A/B/C/D/E/F/G/H 1-243 [» ]
    ProteinModelPortali P63177.
    SMRi P63177. Positions 1-243.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47866N.
    IntActi P63177. 19 interactions.
    STRINGi 511145.b4180.

    Proteomic databases

    PaxDbi P63177.
    PRIDEi P63177.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77137 ; AAC77137 ; b4180 .
    BAE78181 ; BAE78181 ; BAE78181 .
    GeneIDi 12932817.
    948694.
    KEGGi ecj:Y75_p4066.
    eco:b4180.
    PATRICi 32123935. VBIEscCol129921_4312.

    Organism-specific databases

    EchoBASEi EB2376.
    EcoGenei EG12483. rlmB.

    Phylogenomic databases

    eggNOGi COG0566.
    HOGENOMi HOG000218798.
    KOi K03218.
    OMAi IFGFHAV.
    OrthoDBi EOG6GBMDM.
    PhylomeDBi P63177.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7845-MONOMER.
    ECOL316407:JW4138-MONOMER.
    MetaCyc:G7845-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P63177.
    PROi P63177.

    Gene expression databases

    Genevestigatori P63177.

    Family and domain databases

    Gene3Di 3.30.1330.30. 1 hit.
    3.40.1280.10. 1 hit.
    HAMAPi MF_01887. 23SrRNA_methyltr_B.
    InterProi IPR024915. 23S_rRNA_MeTrfase_RlmB.
    IPR029028. Alpha/beta_knot_MTases.
    IPR029064. L30e-like.
    IPR004441. rRNA_MeTrfase_TrmH.
    IPR001537. SpoU_MeTrfase.
    IPR013123. SpoU_subst-bd.
    IPR029026. tRNA_m1G_MTases_N.
    [Graphical view ]
    Pfami PF00588. SpoU_methylase. 1 hit.
    PF08032. SpoU_sub_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00967. SpoU_sub_bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55315. SSF55315. 1 hit.
    SSF75217. SSF75217. 1 hit.
    TIGRFAMsi TIGR00186. rRNA_methyl_3. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli."
      Loevgren J.M., Wikstroem P.M.
      J. Bacteriol. 183:6957-6960(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    5. "The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot."
      Michel G., Sauve V., Larocque R., Li Y., Matte A., Cygler M.
      Structure 10:1303-1315(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT.
      Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.

    Entry informationi

    Entry nameiRLMB_ECOLI
    AccessioniPrimary (citable) accession number: P63177
    Secondary accession number(s): P39290, Q2M6C5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3