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P63177

- RLMB_ECOLI

UniProt

P63177 - RLMB_ECOLI

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Protein

23S rRNA (guanosine-2'-O-)-methyltransferase RlmB

Gene
rlmB, yjfH, b4180, JW4138
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically methylates the ribose of guanosine 2251 in 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei196 – 1961S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei216 – 2161S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei225 – 2251S-adenosyl-L-methionine; via amide nitrogen By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. rRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  1. enzyme-directed rRNA 2'-O-methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7845-MONOMER.
ECOL316407:JW4138-MONOMER.
MetaCyc:G7845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (EC:2.1.1.185)
Alternative name(s):
23S rRNA (guanosine2251 2'-O)-methyltransferase
23S rRNA Gm2251 2'-O-methyltransferase
Gene namesi
Name:rlmB
Synonyms:yjfH
Ordered Locus Names:b4180, JW4138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12483. rlmB.

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24324323S rRNA (guanosine-2'-O-)-methyltransferase RlmBUniRule annotationPRO_0000159785Add
BLAST

Proteomic databases

PaxDbiP63177.
PRIDEiP63177.

Expressioni

Gene expression databases

GenevestigatoriP63177.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-47866N.
IntActiP63177. 19 interactions.
STRINGi511145.b4180.

Structurei

Secondary structure

1
243
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75
Helixi8 – 169
Helixi19 – 213
Beta strandi22 – 309
Turni34 – 363
Helixi37 – 4610
Beta strandi49 – 535
Helixi56 – 605
Turni61 – 633
Beta strandi70 – 745
Helixi82 – 843
Helixi85 – 906
Beta strandi96 – 1027
Helixi106 – 11914
Beta strandi122 – 13110
Helixi136 – 1427
Helixi145 – 1484
Beta strandi151 – 1555
Helixi157 – 16610
Beta strandi170 – 1745
Beta strandi179 – 1813
Helixi182 – 1843
Beta strandi189 – 1979
Turni198 – 2003
Helixi204 – 2085
Beta strandi211 – 2155
Beta strandi219 – 2224
Helixi227 – 24115

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
ProteinModelPortaliP63177.
SMRiP63177. Positions 1-243.

Miscellaneous databases

EvolutionaryTraceiP63177.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0566.
HOGENOMiHOG000218798.
KOiK03218.
OMAiIFGFHAV.
OrthoDBiEOG6GBMDM.
PhylomeDBiP63177.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_01887. 23SrRNA_methyltr_B.
InterProiIPR024915. 23S_rRNA_MeTrfase_RlmB.
IPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR004441. rRNA_MeTrfase_TrmH.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view]
SMARTiSM00967. SpoU_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00186. rRNA_methyl_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P63177-1 [UniParc]FASTAAdd to Basket

« Hide

MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI    50
QLANRQYLDE KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL 100
DGVTDPHNLG ACLRSADAAG VHAVIVPKDR SAQLNATAKK VACGAAESVP 150
LIRVTNLART MRMLQEENIW IVGTAGEADH TLYQSKMTGR LALVMGAEGE 200
GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR QRS 243
Length:243
Mass (Da):26,557
Last modified:September 27, 2004 - v1
Checksum:i6D6C4170580341D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14003 Genomic DNA. Translation: AAA97076.1.
U00096 Genomic DNA. Translation: AAC77137.1.
AP009048 Genomic DNA. Translation: BAE78181.1.
PIRiS56405.
RefSeqiNP_418601.1. NC_000913.3.
YP_492322.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77137; AAC77137; b4180.
BAE78181; BAE78181; BAE78181.
GeneIDi12932817.
948694.
KEGGiecj:Y75_p4066.
eco:b4180.
PATRICi32123935. VBIEscCol129921_4312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14003 Genomic DNA. Translation: AAA97076.1 .
U00096 Genomic DNA. Translation: AAC77137.1 .
AP009048 Genomic DNA. Translation: BAE78181.1 .
PIRi S56405.
RefSeqi NP_418601.1. NC_000913.3.
YP_492322.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GZ0 X-ray 2.50 A/B/C/D/E/F/G/H 1-243 [» ]
ProteinModelPortali P63177.
SMRi P63177. Positions 1-243.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47866N.
IntActi P63177. 19 interactions.
STRINGi 511145.b4180.

Proteomic databases

PaxDbi P63177.
PRIDEi P63177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77137 ; AAC77137 ; b4180 .
BAE78181 ; BAE78181 ; BAE78181 .
GeneIDi 12932817.
948694.
KEGGi ecj:Y75_p4066.
eco:b4180.
PATRICi 32123935. VBIEscCol129921_4312.

Organism-specific databases

EchoBASEi EB2376.
EcoGenei EG12483. rlmB.

Phylogenomic databases

eggNOGi COG0566.
HOGENOMi HOG000218798.
KOi K03218.
OMAi IFGFHAV.
OrthoDBi EOG6GBMDM.
PhylomeDBi P63177.

Enzyme and pathway databases

BioCyci EcoCyc:G7845-MONOMER.
ECOL316407:JW4138-MONOMER.
MetaCyc:G7845-MONOMER.

Miscellaneous databases

EvolutionaryTracei P63177.
PROi P63177.

Gene expression databases

Genevestigatori P63177.

Family and domain databases

Gene3Di 3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
HAMAPi MF_01887. 23SrRNA_methyltr_B.
InterProi IPR024915. 23S_rRNA_MeTrfase_RlmB.
IPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR004441. rRNA_MeTrfase_TrmH.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view ]
Pfami PF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view ]
SMARTi SM00967. SpoU_sub_bind. 1 hit.
[Graphical view ]
SUPFAMi SSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.
TIGRFAMsi TIGR00186. rRNA_methyl_3. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli."
    Loevgren J.M., Wikstroem P.M.
    J. Bacteriol. 183:6957-6960(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  5. "The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot."
    Michel G., Sauve V., Larocque R., Li Y., Matte A., Cygler M.
    Structure 10:1303-1315(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT.
    Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.

Entry informationi

Entry nameiRLMB_ECOLI
AccessioniPrimary (citable) accession number: P63177
Secondary accession number(s): P39290, Q2M6C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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