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P63177 (RLMB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB

EC=2.1.1.185
Alternative name(s):
23S rRNA (guanosine2251 2'-O)-methyltransferase
23S rRNA Gm2251 2'-O-methyltransferase
Gene names
Name:rlmB
Synonyms:yjfH
Ordered Locus Names:b4180, JW4138
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically methylates the ribose of guanosine 2251 in 23S rRNA. Ref.4

Catalytic activity

S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA. Ref.4

Subunit structure

Homodimer. Ref.5

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01887.

Sequence similarities

Belongs to the RNA methyltransferase TrmH family. RlmB subfamily.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processenzyme-directed rRNA 2'-O-methylation

Inferred from mutant phenotype Ref.4. Source: EcoCyc

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

rRNA (guanosine-2'-O-)-methyltransferase activity

Inferred from mutant phenotype Ref.4. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 24324323S rRNA (guanosine-2'-O-)-methyltransferase RlmB HAMAP-Rule MF_01887
PRO_0000159785

Sites

Binding site1961S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2161S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Binding site2251S-adenosyl-L-methionine; via amide nitrogen By similarity

Secondary structure

.................................................. 243
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P63177 [UniParc].

Last modified September 27, 2004. Version 1.
Checksum: 6D6C4170580341D6

FASTA24326,557
        10         20         30         40         50         60 
MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI QLANRQYLDE 

        70         80         90        100        110        120 
KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL DGVTDPHNLG ACLRSADAAG 

       130        140        150        160        170        180 
VHAVIVPKDR SAQLNATAKK VACGAAESVP LIRVTNLART MRMLQEENIW IVGTAGEADH 

       190        200        210        220        230        240 
TLYQSKMTGR LALVMGAEGE GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR 


QRS 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli."
Loevgren J.M., Wikstroem P.M.
J. Bacteriol. 183:6957-6960(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
[5]"The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot."
Michel G., Sauve V., Larocque R., Li Y., Matte A., Cygler M.
Structure 10:1303-1315(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT.
Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14003 Genomic DNA. Translation: AAA97076.1.
U00096 Genomic DNA. Translation: AAC77137.1.
AP009048 Genomic DNA. Translation: BAE78181.1.
PIRS56405.
RefSeqNP_418601.1. NC_000913.3.
YP_492322.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
ProteinModelPortalP63177.
SMRP63177. Positions 1-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47866N.
IntActP63177. 19 interactions.
STRING511145.b4180.

Proteomic databases

PaxDbP63177.
PRIDEP63177.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77137; AAC77137; b4180.
BAE78181; BAE78181; BAE78181.
GeneID12932817.
948694.
KEGGecj:Y75_p4066.
eco:b4180.
PATRIC32123935. VBIEscCol129921_4312.

Organism-specific databases

EchoBASEEB2376.
EcoGeneEG12483. rlmB.

Phylogenomic databases

eggNOGCOG0566.
HOGENOMHOG000218798.
KOK03218.
OMAIFGFHAV.
OrthoDBEOG6GBMDM.
PhylomeDBP63177.

Enzyme and pathway databases

BioCycEcoCyc:G7845-MONOMER.
ECOL316407:JW4138-MONOMER.
MetaCyc:G7845-MONOMER.

Gene expression databases

GenevestigatorP63177.

Family and domain databases

Gene3D3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
HAMAPMF_01887. 23SrRNA_methyltr_B.
InterProIPR024915. 23S_rRNA_MeTrfase_RlmB.
IPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR004441. rRNA_MeTrfase_TrmH.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamPF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view]
SMARTSM00967. SpoU_sub_bind. 1 hit.
[Graphical view]
SUPFAMSSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.
TIGRFAMsTIGR00186. rRNA_methyl_3. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP63177.
PROP63177.

Entry information

Entry nameRLMB_ECOLI
AccessionPrimary (citable) accession number: P63177
Secondary accession number(s): P39290, Q2M6C5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene