Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

23S rRNA (guanosine-2'-O-)-methyltransferase RlmB

Gene

rlmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the ribose of guanosine 2251 in 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei216S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei225S-adenosyl-L-methionine; via amide nitrogenBy similarity1

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • rRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • enzyme-directed rRNA 2'-O-methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7845-MONOMER.
ECOL316407:JW4138-MONOMER.
MetaCyc:G7845-MONOMER.
BRENDAi2.1.1.185. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (EC:2.1.1.185)
Alternative name(s):
23S rRNA (guanosine2251 2'-O)-methyltransferase
23S rRNA Gm2251 2'-O-methyltransferase
Gene namesi
Name:rlmB
Synonyms:yjfH
Ordered Locus Names:b4180, JW4138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12483. rlmB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597851 – 24323S rRNA (guanosine-2'-O-)-methyltransferase RlmBAdd BLAST243

Proteomic databases

PaxDbiP63177.
PRIDEiP63177.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4261811. 9 interactors.
DIPiDIP-47866N.
IntActiP63177. 19 interactors.
STRINGi511145.b4180.

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi8 – 16Combined sources9
Helixi19 – 21Combined sources3
Beta strandi22 – 30Combined sources9
Turni34 – 36Combined sources3
Helixi37 – 46Combined sources10
Beta strandi49 – 53Combined sources5
Helixi56 – 60Combined sources5
Turni61 – 63Combined sources3
Beta strandi70 – 74Combined sources5
Helixi82 – 84Combined sources3
Helixi85 – 90Combined sources6
Beta strandi96 – 102Combined sources7
Helixi106 – 119Combined sources14
Beta strandi122 – 131Combined sources10
Helixi136 – 142Combined sources7
Helixi145 – 148Combined sources4
Beta strandi151 – 155Combined sources5
Helixi157 – 166Combined sources10
Beta strandi170 – 174Combined sources5
Beta strandi179 – 181Combined sources3
Helixi182 – 184Combined sources3
Beta strandi189 – 197Combined sources9
Turni198 – 200Combined sources3
Helixi204 – 208Combined sources5
Beta strandi211 – 215Combined sources5
Beta strandi219 – 222Combined sources4
Helixi227 – 241Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
ProteinModelPortaliP63177.
SMRiP63177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63177.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C2N. Bacteria.
COG0566. LUCA.
HOGENOMiHOG000218798.
InParanoidiP63177.
KOiK03218.
OMAiWVEGVHQ.
PhylomeDBiP63177.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_01887. 23SrRNA_methyltr_B. 1 hit.
InterProiIPR024915. 23S_rRNA_MeTrfase_RlmB.
IPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR004441. rRNA_MeTrfase_TrmH.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view]
SMARTiSM00967. SpoU_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00186. rRNA_methyl_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P63177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI
60 70 80 90 100
QLANRQYLDE KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL
110 120 130 140 150
DGVTDPHNLG ACLRSADAAG VHAVIVPKDR SAQLNATAKK VACGAAESVP
160 170 180 190 200
LIRVTNLART MRMLQEENIW IVGTAGEADH TLYQSKMTGR LALVMGAEGE
210 220 230 240
GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR QRS
Length:243
Mass (Da):26,557
Last modified:September 27, 2004 - v1
Checksum:i6D6C4170580341D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97076.1.
U00096 Genomic DNA. Translation: AAC77137.1.
AP009048 Genomic DNA. Translation: BAE78181.1.
PIRiS56405.
RefSeqiNP_418601.1. NC_000913.3.
WP_001293282.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77137; AAC77137; b4180.
BAE78181; BAE78181; BAE78181.
GeneIDi948694.
KEGGiecj:JW4138.
eco:b4180.
PATRICi32123935. VBIEscCol129921_4312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97076.1.
U00096 Genomic DNA. Translation: AAC77137.1.
AP009048 Genomic DNA. Translation: BAE78181.1.
PIRiS56405.
RefSeqiNP_418601.1. NC_000913.3.
WP_001293282.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
ProteinModelPortaliP63177.
SMRiP63177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261811. 9 interactors.
DIPiDIP-47866N.
IntActiP63177. 19 interactors.
STRINGi511145.b4180.

Proteomic databases

PaxDbiP63177.
PRIDEiP63177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77137; AAC77137; b4180.
BAE78181; BAE78181; BAE78181.
GeneIDi948694.
KEGGiecj:JW4138.
eco:b4180.
PATRICi32123935. VBIEscCol129921_4312.

Organism-specific databases

EchoBASEiEB2376.
EcoGeneiEG12483. rlmB.

Phylogenomic databases

eggNOGiENOG4105C2N. Bacteria.
COG0566. LUCA.
HOGENOMiHOG000218798.
InParanoidiP63177.
KOiK03218.
OMAiWVEGVHQ.
PhylomeDBiP63177.

Enzyme and pathway databases

BioCyciEcoCyc:G7845-MONOMER.
ECOL316407:JW4138-MONOMER.
MetaCyc:G7845-MONOMER.
BRENDAi2.1.1.185. 2026.

Miscellaneous databases

EvolutionaryTraceiP63177.
PROiP63177.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_01887. 23SrRNA_methyltr_B. 1 hit.
InterProiIPR024915. 23S_rRNA_MeTrfase_RlmB.
IPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR004441. rRNA_MeTrfase_TrmH.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view]
SMARTiSM00967. SpoU_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00186. rRNA_methyl_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLMB_ECOLI
AccessioniPrimary (citable) accession number: P63177
Secondary accession number(s): P39290, Q2M6C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.