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Protein

23S rRNA (guanosine-2'-O-)-methyltransferase RlmB

Gene

rlmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the ribose of guanosine 2251 in 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanosine(2251) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(2251) in 23S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei216S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei225S-adenosyl-L-methionine; via amide nitrogenBy similarity1

GO - Molecular functioni

  • RNA binding Source: InterPro
  • rRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • enzyme-directed rRNA 2'-O-methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7845-MONOMER
MetaCyc:G7845-MONOMER
BRENDAi2.1.1.185 2026

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (EC:2.1.1.185)
Alternative name(s):
23S rRNA (guanosine2251 2'-O)-methyltransferase
23S rRNA Gm2251 2'-O-methyltransferase
Gene namesi
Name:rlmB
Synonyms:yjfH
Ordered Locus Names:b4180, JW4138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12483 rlmB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597851 – 24323S rRNA (guanosine-2'-O-)-methyltransferase RlmBAdd BLAST243

Proteomic databases

PaxDbiP63177
PRIDEiP63177

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4261811, 37 interactors
DIPiDIP-47866N
IntActiP63177, 19 interactors
STRINGi316385.ECDH10B_4375

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi8 – 16Combined sources9
Helixi19 – 21Combined sources3
Beta strandi22 – 30Combined sources9
Turni34 – 36Combined sources3
Helixi37 – 46Combined sources10
Beta strandi49 – 53Combined sources5
Helixi56 – 60Combined sources5
Turni61 – 63Combined sources3
Beta strandi70 – 74Combined sources5
Helixi82 – 84Combined sources3
Helixi85 – 90Combined sources6
Beta strandi96 – 102Combined sources7
Helixi106 – 119Combined sources14
Beta strandi122 – 131Combined sources10
Helixi136 – 142Combined sources7
Helixi145 – 148Combined sources4
Beta strandi151 – 155Combined sources5
Helixi157 – 166Combined sources10
Beta strandi170 – 174Combined sources5
Beta strandi179 – 181Combined sources3
Helixi182 – 184Combined sources3
Beta strandi189 – 197Combined sources9
Turni198 – 200Combined sources3
Helixi204 – 208Combined sources5
Beta strandi211 – 215Combined sources5
Beta strandi219 – 222Combined sources4
Helixi227 – 241Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
ProteinModelPortaliP63177
SMRiP63177
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63177

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C2N Bacteria
COG0566 LUCA
HOGENOMiHOG000218798
InParanoidiP63177
KOiK03218
OMAiWVEGVHQ
PhylomeDBiP63177

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.40.1280.10, 1 hit
HAMAPiMF_01887 23SrRNA_methyltr_B, 1 hit
InterProiView protein in InterPro
IPR024915 23S_rRNA_MeTrfase_RlmB
IPR029028 Alpha/beta_knot_MTases
IPR029064 L30e-like
IPR004441 rRNA_MeTrfase_TrmH
IPR001537 SpoU_MeTrfase
IPR013123 SpoU_subst-bd
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR12029:SF58 PTHR12029:SF58, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PF08032 SpoU_sub_bind, 1 hit
SMARTiView protein in SMART
SM00967 SpoU_sub_bind, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00186 rRNA_methyl_3, 1 hit

Sequencei

Sequence statusi: Complete.

P63177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI
60 70 80 90 100
QLANRQYLDE KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL
110 120 130 140 150
DGVTDPHNLG ACLRSADAAG VHAVIVPKDR SAQLNATAKK VACGAAESVP
160 170 180 190 200
LIRVTNLART MRMLQEENIW IVGTAGEADH TLYQSKMTGR LALVMGAEGE
210 220 230 240
GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR QRS
Length:243
Mass (Da):26,557
Last modified:September 27, 2004 - v1
Checksum:i6D6C4170580341D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97076.1
U00096 Genomic DNA Translation: AAC77137.1
AP009048 Genomic DNA Translation: BAE78181.1
PIRiS56405
RefSeqiNP_418601.1, NC_000913.3
WP_001293282.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77137; AAC77137; b4180
BAE78181; BAE78181; BAE78181
GeneIDi948694
KEGGiecj:JW4138
eco:b4180
PATRICifig|1411691.4.peg.2521

Similar proteinsi

Entry informationi

Entry nameiRLMB_ECOLI
AccessioniPrimary (citable) accession number: P63177
Secondary accession number(s): P39290, Q2M6C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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