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P63172 (DYLT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynein light chain Tctex-type 1
Alternative name(s):
Protein CW-1
T-complex testis-specific protein 1 homolog
Gene names
Name:DYNLT1
Synonyms:TCTEL1, TCTEX-1, TCTEX1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length113 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein. Ref.14

Plays a role in neuronal morphogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation By similarity. Ref.14

Subunit structure

Homodimer Probable. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with DYNC1I1 and DYNC1I2. Interacts with RHO. Interacts with DOC2A, DOC2B and SCN10A. Interacts with PVR. Interacts with SVIL isoform 2 Interacts with BMPR2. Interacts with GNB1; the interaction occurs in presence of guanine nucleotide-binding protein G(T) subunit gamma; the interaction diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in presence of guanine nucleotide-binding protein G(T) subunit gamma By similarity. Interacts with human papillomavirus 16 L2 protein; this interaction is essential for virus intracellular transport during entry. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.17

Subcellular location

Golgi apparatus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity. Note: Localizes to mitotic spindles By similarity.

Tissue specificity

Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level). Ref.10

Post-translational modification

Phosphorylated by BMPR2; the phosphorylation is abolished by BMPR2 mutations in exon 12 which lead to truncated forms of BMPR2 and which are linked to primary pulmonary hypertension (PPH1) [MIM:178600]. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release By similarity. Ref.10

Sequence similarities

Belongs to the dynein light chain Tctex-type family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Cytoplasmic inwards viral transport
Host-virus interaction
Microtubular inwards viral transport
Mitosis
Neurogenesis
Transport
Virus entry into host cell
   Cellular componentCytoplasm
Cytoskeleton
Dynein
Golgi apparatus
Microtubule
   Molecular functionMotor protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processestablishment of mitotic spindle orientation

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular transport of viral protein in host cell

Inferred from mutant phenotype Ref.14. Source: UniProtKB

microtubule-dependent intracellular transport of viral material towards nucleus

Inferred from electronic annotation. Source: UniProtKB-KW

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of neurogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

neuron projection morphogenesis

Inferred from electronic annotation. Source: Ensembl

regulation of G-protein coupled receptor protein signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of Rac GTPase activity

Inferred from electronic annotation. Source: Ensembl

regulation of cytoskeleton organization

Inferred from electronic annotation. Source: Ensembl

viral entry into host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic dynein complex

Inferred from sequence or structural similarity. Source: UniProtKB

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

spindle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionidentical protein binding

Inferred from physical interaction Ref.13. Source: UniProtKB

motor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSPB9Q9BQS65EBI-1176455,EBI-1176448

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 113113Dynein light chain Tctex-type 1
PRO_0000195152

Regions

Region41 – 11373Interaction with GNB1 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.15 Ref.18

Sequences

Sequence LengthMass (Da)Tools
P63172 [UniParc].

Last modified September 27, 2004. Version 1.
Checksum: A6BAD1D0744D1AA6

FASTA11312,452
        10         20         30         40         50         60 
MEDYQAAEET AFVVDEVSNI VKEAIESAIG GNAYQHSKVN QWTTNVVEQT LSQLTKLGKP 

        70         80         90        100        110 
FKYIVTCVIM QKNGAGLHTA SSCFWDSSTD GSCTVRWENK TMYCIVSAFG LSI 

« Hide

References

« Hide 'large scale' references
[1]Cao X.M., Solecki D.J., Wimmer E.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning, expression, and mapping of TCTEL1, a putative human homologue of murine Tcte1, to 6q."
Watanabe T.K., Fujiwara T., Shimizu F., Okuno S., Suzuki M., Takahashi E., Nakamura Y., Hirai Y.
Cytogenet. Cell Genet. 73:153-156(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal brain.
[3]Indu S., Laloraya M., Kumar P.G.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[9]"Interaction of the poliovirus receptor CD155 with the dynein light chain Tctex-1 and its implication for poliovirus pathogenesis."
Mueller S., Cao X., Welker R., Wimmer E.
J. Biol. Chem. 277:7897-7904(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PVR.
[10]"Functional interaction between BMPR-II and Tctex-1, a light chain of Dynein, is isoform-specific and disrupted by mutations underlying primary pulmonary hypertension."
Machado R.D., Rudarakanchana N., Atkinson C., Flanagan J.A., Harrison R., Morrell N.W., Trembath R.C.
Hum. Mol. Genet. 12:3277-3286(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BMPR2, PHOSPHORYLATION, TISSUE SPECIFICITY.
[11]"Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome."
Ohka S., Matsuda N., Tohyama K., Oda T., Morikawa M., Kuge S., Nomoto A.
J. Virol. 78:7186-7198(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PVR.
[12]"Supervillin modulation of focal adhesions involving TRIP6/ZRP-1."
Takizawa N., Smith T.C., Nebl T., Crowley J.L., Palmieri S.J., Lifshitz L.M., Ehrhardt A.G., Hoffman L.M., Beckerle M.C., Luna E.J.
J. Cell Biol. 174:447-458(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SVIL.
[13]"Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex."
Lo K.W., Kogoy J.M., Rasoul B.A., King S.M., Pfister K.K.
J. Biol. Chem. 282:36871-36878(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SELF-ASSOCIATION, INTERACTION WITH DYNC1I1 AND DYNC1I2, SUBUNIT.
[14]"D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein."
Vlach J., Lipov J., Rumlova M., Veverka V., Lang J., Srb P., Knejzlik Z., Pichova I., Hunter E., Hrabal R., Ruml T.
Proc. Natl. Acad. Sci. U.S.A. 105:10565-10570(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN INTRACELLULAR TRANSPORT OF D-TYPE RETROVIRUS GAG POLYPROTEIN, INTERACTION WITH MPMV GAG.
[15]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"Identification of the dynein light chains required for human papillomavirus infection."
Schneider M.A., Spoden G.A., Florin L., Lambert C.
Cell. Microbiol. 13:32-46(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN PAPILLOMAVIRUS 16 L2 PROTEIN.
[18]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U56255 mRNA. Translation: AAB03318.1.
D50663 mRNA. Translation: BAA09317.1.
EU862237 mRNA. Translation: ACF74976.1.
AK315601 mRNA. Translation: BAG37971.1.
CR456931 mRNA. Translation: CAG33212.1.
AL591025, AL589931 Genomic DNA. Translation: CAH70176.1.
AL589931, AL591025 Genomic DNA. Translation: CAI95303.1.
CH471051 Genomic DNA. Translation: EAW47657.1.
BC029412 mRNA. Translation: AAH29412.1.
BC105588 mRNA. Translation: AAI05589.1.
RefSeqNP_006510.1. NM_006519.2.
UniGeneHs.445999.

3D structure databases

ProteinModelPortalP63172.
SMRP63172. Positions 12-113.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112853. 19 interactions.
DIPDIP-35251N.
IntActP63172. 11 interactions.
STRING9606.ENSP00000356056.

PTM databases

PhosphoSiteP63172.

Polymorphism databases

DMDM52783582.

Proteomic databases

PaxDbP63172.
PRIDEP63172.

Protocols and materials databases

DNASU6993.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000367089; ENSP00000356056; ENSG00000146425.
GeneID6993.
KEGGhsa:6993.
UCSCuc003qrn.2. human.

Organism-specific databases

CTD6993.
GeneCardsGC06M159057.
HGNCHGNC:11697. DYNLT1.
MIM601554. gene.
neXtProtNX_P63172.
PharmGKBPA36416.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG278055.
HOGENOMHOG000255148.
HOVERGENHBG001217.
InParanoidP63172.
KOK10420.
OMASCFWDNS.
PhylomeDBP63172.
TreeFamTF313904.

Gene expression databases

ArrayExpressP63172.
BgeeP63172.
CleanExHS_DYNLT1.
GenevestigatorP63172.

Family and domain databases

InterProIPR005334. Tctex.
[Graphical view]
PANTHERPTHR21255. PTHR21255. 1 hit.
PfamPF03645. Tctex-1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDYNLT1. human.
GeneWikiDYNLT1.
GenomeRNAi6993.
NextBio27321.
PROP63172.
SOURCESearch...

Entry information

Entry nameDYLT1_HUMAN
AccessionPrimary (citable) accession number: P63172
Secondary accession number(s): Q15763, Q5VTU4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM