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Protein

Dynein light chain Tctex-type 1

Gene

DYNLT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. May also be a accessory component of axonemal dynein.
Plays a role in neuronal morphogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation (By similarity). Unrelated to the role in retrograde microtubule-associated movement may play a role in the dimerization of cytoplasmic proteins/domains such as for ACVR2B. Binds to the cytoplasmic domain of ACVR2B and, in vitro, inhibits ACVR2B signaling (PubMed:27502274).By similarity1 Publication
(Microbial infection) Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • motor activity Source: UniProtKB-KW
  • protein C-terminus binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionMotor protein
Biological processCell cycle, Cell division, Cytoplasmic inwards viral transport, Host-virus interaction, Microtubular inwards viral transport, Mitosis, Neurogenesis, Transport, Virus entry into host cell

Enzyme and pathway databases

ReactomeiR-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain Tctex-type 1
Alternative name(s):
Protein CW-1
T-complex testis-specific protein 1 homolog
Gene namesi
Name:DYNLT1
Synonyms:TCTEL1, TCTEX-1, TCTEX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000146425.10.
HGNCiHGNC:11697. DYNLT1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Golgi apparatus, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi6993.
OpenTargetsiENSG00000146425.
PharmGKBiPA36416.

Polymorphism and mutation databases

BioMutaiDYNLT1.
DMDMi52783582.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951521 – 113Dynein light chain Tctex-type 1Add BLAST113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Post-translational modificationi

Phosphorylated by BMPR2; the phosphorylation is abolished by BMPR2 mutations in exon 12 which lead to truncated forms of BMPR2 and which are linked to primary pulmonary hypertension (PPH1) [MIMi:178600]. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63172.
PaxDbiP63172.
PeptideAtlasiP63172.
PRIDEiP63172.
TopDownProteomicsiP63172.

PTM databases

iPTMnetiP63172.
PhosphoSitePlusiP63172.

Expressioni

Tissue specificityi

Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000146425.
CleanExiHS_DYNLT1.
ExpressionAtlasiP63172. baseline and differential.
GenevisibleiP63172. HS.

Organism-specific databases

HPAiHPA046559.

Interactioni

Subunit structurei

Homodimer (Probable). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with DYNC1I1 and DYNC1I2. Interacts with RHO. Interacts with DOC2A, DOC2B and SCN10A. Interacts with PVR. Interacts with SVIL isoform 2. Interacts with BMPR2. Interacts with GNB1; the interaction occurs in presence of guanine nucleotide-binding protein G(T) subunit gamma; the interaction diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in presence of guanine nucleotide-binding protein G(T) subunit gamma (By similarity). Interacts with ACVR2B and ARHGEF2. Interacts with human papillomavirus 16 L2 protein; this interaction is essential for virus intracellular transport during entry.By similarityCurated8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • protein C-terminus binding Source: Ensembl

Protein-protein interaction databases

BioGridi112853. 110 interactors.
CORUMiP63172.
DIPiDIP-35251N.
IntActiP63172. 51 interactors.
STRINGi9606.ENSP00000356056.

Structurei

Secondary structure

1113
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 3Combined sources3
Turni8 – 10Combined sources3
Helixi14 – 29Combined sources16
Helixi38 – 57Combined sources20
Beta strandi62 – 71Combined sources10
Turni72 – 74Combined sources3
Beta strandi78 – 81Combined sources4
Beta strandi87 – 98Combined sources12
Beta strandi100 – 111Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JPWNMR-A/B1-113[»]
ProteinModelPortaliP63172.
SMRiP63172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 113Interaction with GNB1By similarityAdd BLAST73

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4081. Eukaryota.
ENOG4111NHR. LUCA.
GeneTreeiENSGT00390000013998.
HOGENOMiHOG000255148.
HOVERGENiHBG001217.
InParanoidiP63172.
KOiK10420.
OMAiCYWDNAT.
OrthoDBiEOG091G0Y5N.
PhylomeDBiP63172.
TreeFamiTF313904.

Family and domain databases

InterProiView protein in InterPro
IPR005334. Tctex.
PANTHERiPTHR21255. PTHR21255. 1 hit.
PfamiView protein in Pfam
PF03645. Tctex-1. 1 hit.

Sequencei

Sequence statusi: Complete.

P63172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDYQAAEET AFVVDEVSNI VKEAIESAIG GNAYQHSKVN QWTTNVVEQT
60 70 80 90 100
LSQLTKLGKP FKYIVTCVIM QKNGAGLHTA SSCFWDSSTD GSCTVRWENK
110
TMYCIVSAFG LSI
Length:113
Mass (Da):12,452
Last modified:September 27, 2004 - v1
Checksum:iA6BAD1D0744D1AA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56255 mRNA. Translation: AAB03318.1.
D50663 mRNA. Translation: BAA09317.1.
EU862237 mRNA. Translation: ACF74976.1.
AK315601 mRNA. Translation: BAG37971.1.
CR456931 mRNA. Translation: CAG33212.1.
AL591025 Genomic DNA. No translation available.
AL589931 Genomic DNA. No translation available.
CH471051 Genomic DNA. Translation: EAW47657.1.
BC029412 mRNA. Translation: AAH29412.1.
BC105588 mRNA. Translation: AAI05589.1.
CCDSiCCDS5257.1.
RefSeqiNP_006510.1. NM_006519.3.
UniGeneiHs.445999.

Genome annotation databases

EnsembliENST00000367089; ENSP00000356056; ENSG00000146425.
GeneIDi6993.
KEGGihsa:6993.
UCSCiuc003qrn.3. human.

Similar proteinsi

Entry informationi

Entry nameiDYLT1_HUMAN
AccessioniPrimary (citable) accession number: P63172
Secondary accession number(s): Q15763, Q5VTU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 22, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families