Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dynein light chain 1, cytoplasmic

Gene

Dynll1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity).By similarity
Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.By similarity
Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.By similarity
Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity (By similarity).By similarity

GO - Molecular functioni

  • cytoskeletal protein binding Source: GO_Central
  • dynein intermediate chain binding Source: MGI
  • dynein light intermediate chain binding Source: GO_Central
  • enzyme inhibitor activity Source: MGI
  • motor activity Source: UniProtKB-KW
  • protein C-terminus binding Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActivator, Motor protein
Biological processApoptosis, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-111446 Activation of BIM and translocation to mitochondria
R-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-1632852 Macroautophagy
R-MMU-2132295 MHC class II antigen presentation
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5620924 Intraflagellar transport
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6798695 Neutrophil degranulation
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-MMU-68877 Mitotic Prometaphase
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain 1, cytoplasmic
Alternative name(s):
8 kDa dynein light chain
Short name:
DLC8
Dynein light chain LC8-type 1
Protein inhibitor of neuronal nitric oxide synthase
Short name:
PIN
Short name:
mPIN
Gene namesi
Name:Dynll1
Synonyms:Dlc1, Dncl1, Dnclc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1861457 Dynll1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951271 – 89Dynein light chain 1, cytoplasmicAdd BLAST89

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36N6-acetyllysineCombined sources1
Cross-linki43Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei88PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-88 appears to control the dimer-monomer transition.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63168
MaxQBiP63168
PaxDbiP63168
PeptideAtlasiP63168
PRIDEiP63168
TopDownProteomicsiP63168

PTM databases

iPTMnetiP63168
PhosphoSitePlusiP63168

Expressioni

Gene expression databases

BgeeiENSMUSG00000009013
CleanExiMM_DLC1
MM_DYNLL1
GenevisibleiP63168 MM

Interactioni

Subunit structurei

Homodimer. Monomer; the monomeric form is incapable of binding to target proteins. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with TXNDC17. Interacts with WWC1 and ESR1. The interaction with WWC1 is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin. Interacts with BCL2; the interaction is greatly enhanced in the nucleus and in mitochondria upon induction of apoptosis. Interacts with PAK1; the interaction requires dimeric DYNLL1. Interacts with MYZAP. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2. Interacts with ATMIN; this interaction inhibits ATMIN transcriptional activity and hence may play a role in a feedback loop whereby DYNLL1 inhibits transactivation of its own promoter by ATMIN. Interacts with NEK9 (not phosphorylated at 'Ser-944') (By similarity). Interacts with BCL2L11 (PubMed:21478148). Interacts with BICD2 (PubMed:22956769).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • cytoskeletal protein binding Source: GO_Central
  • dynein intermediate chain binding Source: MGI
  • dynein light intermediate chain binding Source: GO_Central
  • protein C-terminus binding Source: MGI
  • protein domain specific binding Source: MGI

Protein-protein interaction databases

BioGridi207994, 75 interactors
ELMiP63168
IntActiP63168, 76 interactors
MINTiP63168
STRINGi10090.ENSMUSP00000009157

Structurei

3D structure databases

ProteinModelPortaliP63168
SMRiP63168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 89Interaction with ESR1By similarityAdd BLAST23

Sequence similaritiesi

Belongs to the dynein light chain family.Curated

Phylogenomic databases

eggNOGiKOG3430 Eukaryota
ENOG4111NK2 LUCA
GeneTreeiENSGT00390000000378
HOVERGENiHBG002133
InParanoidiP63168
KOiK10418
OMAiQAIMSDR
OrthoDBiEOG091G15JY
PhylomeDBiP63168
TreeFamiTF300264

Family and domain databases

Gene3Di3.30.740.10, 1 hit
InterProiView protein in InterPro
IPR037177 DLC_sf
IPR019763 Dynein_light_1/2_CS
IPR001372 Dynein_light_chain_typ-1/2
PANTHERiPTHR11886 PTHR11886, 1 hit
PfamiView protein in Pfam
PF01221 Dynein_light, 1 hit
SMARTiView protein in SMART
SM01375 Dynein_light, 1 hit
SUPFAMiSSF54648 SSF54648, 1 hit
PROSITEiView protein in PROSITE
PS01239 DYNEIN_LIGHT_1, 1 hit

Sequencei

Sequence statusi: Complete.

P63168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY
60 70 80
NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
Length:89
Mass (Da):10,366
Last modified:September 27, 2004 - v1
Checksum:iF5E7647D092BEB3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020185 mRNA Translation: AAD01643.1
AK002522 mRNA Translation: BAB22160.1
AK010614 mRNA Translation: BAB27063.1
AK010685 mRNA Translation: BAB27117.1
AK013721 mRNA Translation: BAB28970.1
AK082923 mRNA Translation: BAC38691.1
AK147977 mRNA Translation: BAE28262.1
BC008106 mRNA Translation: AAH08106.1
BC034258 mRNA Translation: AAH34258.1
CCDSiCCDS39227.1
RefSeqiNP_062656.3, NM_019682.4
UniGeneiMm.256858

Genome annotation databases

EnsembliENSMUST00000009157; ENSMUSP00000009157; ENSMUSG00000009013
ENSMUST00000112090; ENSMUSP00000107720; ENSMUSG00000009013
GeneIDi56455
KEGGimmu:56455
UCSCiuc008zdp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDYL1_MOUSE
AccessioniPrimary (citable) accession number: P63168
Secondary accession number(s): Q15701, Q3UGE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: April 25, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health