Reviewed, UniProtKB/Swiss-Prot P63167 (DYL1_HUMAN)

Names and origin

Protein names

Recommended name:
    Dynein light chain 1, cytoplasmic
Alternative name(s):
    Dynein light chain LC8-type 1
    8 kDa dynein light chain
      Short name=DLC8
    Protein inhibitor of neuronal nitric oxide synthase
      Short name=PIN

Gene names

Name:	DYNLL1
Synonyms:	DLC1, DNCL1, DNCLC1, HDLC1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in some aspects of dynein-related intracellular transport and motility. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.
Subunit structure	Consists of at least two heavy chains and a number of intermediate and light chains. Interacts with rabies P protein By similarity. Interacts with TXNDC17. Ref.3
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the dynein light chain family.

Ontologies

Keywords
Cellular component	Cytoplasm Dynein Microtubule
Molecular function	Motor protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Ref.1 Traceable author statement. Source: ProtInc activation of pro-apoptotic gene products Inferred from Experiment. Source: Reactome anatomical structure morphogenesis Ref.1 Traceable author statement. Source: ProtInc female gamete generation Ref.1 Traceable author statement. Source: ProtInc microtubule-based process Inferred from electronic annotation. Source: InterPro negative regulation of phosphorylation Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasmic dynein complex Ref.1 Traceable author statement. Source: ProtInc cytosol Inferred from Experiment. Source: Reactome microtubule Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from Experiment. Source: Reactome
Molecular function	microtubule motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ASF1B	Q9NVP2	1	EBI-349105,EBI-1055650
BCAS1	O75363	1	EBI-349105,EBI-1042966
DYNC1I2	Q13409	1	EBI-349105,EBI-742998
DYNLL2	Q96FJ2	1	EBI-349105,EBI-742371
GNB2L1	P63244	3	EBI-349105,EBI-296739
NEK6	Q9HC98	1	EBI-349105,EBI-740364
PABPC1	P11940	1	EBI-349105,EBI-81531
SNCG	O76070	1	EBI-349105,EBI-1053810

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 89

89

Dynein light chain 1, cytoplasmic

PRO_0000195125

Amino acid modifications

Modified residue

36

1

N6-acetyllysine Ref.6

Modified residue

65

1

Phosphotyrosine Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

89

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

15 – 31

17

Helix

35 – 50

16

Beta strand

54 – 69

16

Beta strand

73 – 78

6

Beta strand

81 – 87

7

Sequences

	Sequence		Length	Mass (Da)
	P63167-1 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: F5E7647D092BEB3A	FASTA	89	10,366
	10 20 30 40 50 60 MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR 70 80 NFGSYVTHET KHFIYFYLGQ VAILLFKSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster." Dick T., Ray K., Salz H.K., Chia W. Mol. Cell. Biol. 16:1966-1977(1996) [PubMed: 8628263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways." Jeong W., Chang T.-S., Boja E.S., Fales H.M., Rhee S.G. J. Biol. Chem. 279:3151-3159(2004) [PubMed: 14607843] [Abstract] Cited for: INTERACTION WITH TXNDC17.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-65, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-36, MASS SPECTROMETRY.
[7]	"Structure of the PIN/LC8 dimer with a bound peptide." Liang J., Jaffrey S.R., Guo W., Snyder S.H., Clardy J. Nat. Struct. Biol. 6:735-740(1999) [PubMed: 10426949] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 5-89.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U32944 mRNA. Translation: AAB04149.1.
CR407672 mRNA. Translation: CAG28600.1.

IPI

IPI00019329.

RefSeq

NP_001032583.1.
NP_001032584.1.
NP_003737.1.

UniGene

Hs.5120

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CMI	X-ray	2.50	A/B	5-89	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P63167. 30 interactions.

STRING

P63167.

PTM databases

PhosphoSite

P63167.

Proteomic databases

PeptideAtlas

P63167.

PRIDE

P63167.

Genome annotation databases

Ensembl

ENST00000242577; ENSP00000242577; ENSG00000088986; Homo sapiens. [Genome view]
ENST00000392508; ENSP00000376296; ENSG00000088986; Homo sapiens. [Genome view]
ENST00000392509; ENSP00000376297; ENSG00000088986; Homo sapiens. [Genome view]

GeneID

8655.

KEGG

hsa:8655.

UCSC

uc001tyj.1. human.

Organism-specific databases

CTD

8655.

GeneCards

GC12P119397.

H-InvDB

HIX0029546.
HIX0034107.
HIX0036835.
HIX0037247.
HIX0059369.

HGNC

HGNC:15476. DYNLL1.

MIM

601562. gene.

PharmGKB

PA134972261.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20803.

HOGENOM

HBG627578.

HOVERGEN

P63167.

InParanoid

P63167.

PhylomeDB

P63167.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_578. Apoptosis.

Gene expression databases

ArrayExpress

P63167.

Bgee

P63167.

CleanEx

HS_DLC1.
HS_DYNLL1.

Genevestigator

P63167.

GermOnline

ENSG00000088986. Homo sapiens.

Family and domain databases

InterPro

IPR019763. Dynein_light_1/2_CS.
IPR001372. Dynein_light_chain_typ-1/2.
[Graphical view]

Gene3D

G3DSA:3.30.740.10. Dynein_light1. 1 hit.

PANTHER

PTHR11886. Dynein_light1. 1 hit.

Pfam

PF01221. Dynein_light. 1 hit.
[Graphical view]

PROSITE

PS01239. DYNEIN_LIGHT_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

32461.

SOURCE

Search...

Entry information

Entry name

DYL1_HUMAN

Accession

Primary (citable) accession number: P63167
Secondary accession number(s): Q15701

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.