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Protein

Small ubiquitin-related modifier 1

Gene

Sumo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to cadmium ion Source: UniProtKB
  • cellular response to heat Source: UniProtKB
  • negative regulation of action potential Source: UniProtKB
  • negative regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • negative regulation of DNA binding Source: MGI
  • negative regulation of DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • palate development Source: UniProtKB
  • PML body organization Source: MGI
  • positive regulation of calcium-transporting ATPase activity Source: BHF-UCL
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  • positive regulation of protein complex assembly Source: MGI
  • protein localization to nuclear pore Source: MGI
  • protein stabilization Source: MGI
  • protein sumoylation Source: MGI
  • regulation of calcium ion transmembrane transport Source: BHF-UCL
  • regulation of cardiac muscle cell contraction Source: BHF-UCL
  • regulation of protein stability Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Biological processStress response, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-3065676 SUMO is conjugated to E1 (UBA2:SAE1)
R-MMU-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-MMU-3065679 SUMO is proteolytically processed
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-3232118 SUMOylation of transcription factors
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-4570464 SUMOylation of RNA binding proteins
R-MMU-4615885 SUMOylation of DNA replication proteins
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-69473 G2/M DNA damage checkpoint
R-MMU-877312 Regulation of IFNG signaling
R-MMU-8866904 Negative regulation of activity of TFAP2 (AP-2) family transcription factors

Names & Taxonomyi

Protein namesi
Recommended name:
Small ubiquitin-related modifier 1
Short name:
SUMO-1
Alternative name(s):
SMT3 homolog 3
Ubiquitin-homology domain protein PIC1
Ubiquitin-like protein SMT3C
Short name:
Smt3C
Gene namesi
Name:Sumo1
Synonyms:Smt3c, Smt3h3, Ubl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1197010 Sumo1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000359412 – 97Small ubiquitin-related modifier 1Add BLAST96
PropeptideiPRO_000003594298 – 101By similarity4

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei9PhosphoserineBy similarity1
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki23Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei32PhosphoserineBy similarity1
Cross-linki37Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki39Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki97Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form by SENP1 or SENP2 is necessary for function.By similarity
Polymeric SUMO1 chains undergo polyubiquitination by RNF4.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63166
MaxQBiP63166
PaxDbiP63166
PeptideAtlasiP63166
PRIDEiP63166

PTM databases

iPTMnetiP63166
PhosphoSitePlusiP63166

Expressioni

Tissue specificityi

Ubiquitous.

Developmental stagei

Expressed at E13.5 strongly in the upper lip, primary palate and medial edge epithelia of the secondary palate. At E14.5 expression could be seen in the medial edge epithelial seam.1 Publication

Inductioni

By hypoxia.1 Publication

Gene expression databases

BgeeiENSMUSG00000026021
CleanExiMM_SUMO1
ExpressionAtlasiP63166 baseline and differential
GenevisibleiP63166 MM

Interactioni

Subunit structurei

Interacts with USP25 (via ts SIM domain) the interaction weakly sumoylates USP25. Covalently attached to KCNB1; UBE2I increases cross-linking with KCNB1 and PIAS1 decreases cross-links with KCNB1 (By similarity). Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Interacts with PRKN. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. Interacts with SIMC1, CASP8AP2, RNF111 AND SOBP (via SIM domains). Interacts with BHLHE40/DEC1. Interacts with RWDD3. Interacts with UBE2I/UBC9 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36Interaction with PIAS2By similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20442022 interactors.
CORUMiP63166
DIPiDIP-29278N
IntActiP63166 14 interactors.
MINTiP63166
STRINGi10090.ENSMUSP00000088935

Structurei

3D structure databases

ProteinModelPortaliP63166
SMRiP63166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 97Ubiquitin-likePROSITE-ProRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the ubiquitin family. SUMO subfamily.Curated

Phylogenomic databases

eggNOGiKOG1769 Eukaryota
COG5227 LUCA
GeneTreeiENSGT00390000018808
HOGENOMiHOG000207495
HOVERGENiHBG053025
InParanoidiP63166
KOiK12160
OMAiTDNHTPK
OrthoDBiEOG091G10ZQ
PhylomeDBiP63166
TreeFamiTF315116

Family and domain databases

CDDicd01763 Sumo, 1 hit
InterProiView protein in InterPro
IPR022617 Rad60/SUMO-like_dom
IPR033950 Sumo
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF11976 Rad60-SLD, 1 hit
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES
60 70 80 90 100
YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST

V
Length:101
Mass (Da):11,557
Last modified:September 27, 2004 - v1
Checksum:i89BE97D2D054FB33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033353 mRNA Translation: AAC39959.1
AK002536 mRNA Translation: BAB22172.1
AK011074 mRNA Translation: BAB27379.1
AK089081 mRNA Translation: BAC40739.1
AK159366 mRNA Translation: BAE35024.1
BC082566 mRNA Translation: AAH82566.1
BC083158 mRNA Translation: AAH83158.1
CCDSiCCDS35586.1
RefSeqiNP_033486.1, NM_009460.2
UniGeneiMm.362118

Genome annotation databases

EnsembliENSMUST00000091374; ENSMUSP00000088935; ENSMUSG00000026021
GeneIDi22218
KEGGimmu:22218
UCSCiuc007bdx.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSUMO1_MOUSE
AccessioniPrimary (citable) accession number: P63166
Secondary accession number(s): P55856, Q3TX92, Q93068
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome