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Protein

Small ubiquitin-related modifier 1

Gene

Sumo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to cadmium ion Source: UniProtKB
  • cellular response to heat Source: UniProtKB
  • negative regulation of action potential Source: UniProtKB
  • negative regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • negative regulation of DNA binding Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • palate development Source: UniProtKB
  • PML body organization Source: MGI
  • positive regulation of calcium-transporting ATPase activity Source: BHF-UCL
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  • positive regulation of protein complex assembly Source: MGI
  • protein localization to nuclear pore Source: MGI
  • protein stabilization Source: MGI
  • protein sumoylation Source: MGI
  • regulation of calcium ion transmembrane transport Source: BHF-UCL
  • regulation of cardiac muscle cell contraction Source: BHF-UCL
  • regulation of protein stability Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Biological processStress response, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-MMU-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).
R-MMU-3065679. SUMO is proteolytically processed.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3232118. SUMOylation of transcription factors.
R-MMU-4551638. SUMOylation of chromatin organization proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-MMU-5693571. Nonhomologous End-Joining (NHEJ).
R-MMU-69473. G2/M DNA damage checkpoint.
R-MMU-877312. Regulation of IFNG signaling.
R-MMU-8866904. Negative regulation of activity of TFAP2 (AP-2) family transcription factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Small ubiquitin-related modifier 1
Short name:
SUMO-1
Alternative name(s):
SMT3 homolog 3
Ubiquitin-homology domain protein PIC1
Ubiquitin-like protein SMT3C
Short name:
Smt3C
Gene namesi
Name:Sumo1
Synonyms:Smt3c, Smt3h3, Ubl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1197010. Sumo1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000359412 – 97Small ubiquitin-related modifier 1Add BLAST96
PropeptideiPRO_000003594298 – 101By similarity4

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei9PhosphoserineBy similarity1
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki23Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei32PhosphoserineBy similarity1
Cross-linki37Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki39Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki97Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form by SENP1 or SENP2 is necessary for function.By similarity
Polymeric SUMO1 chains undergo polyubiquitination by RNF4.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63166.
MaxQBiP63166.
PaxDbiP63166.
PeptideAtlasiP63166.
PRIDEiP63166.

PTM databases

iPTMnetiP63166.
PhosphoSitePlusiP63166.

Expressioni

Tissue specificityi

Ubiquitous.

Developmental stagei

Expressed at E13.5 strongly in the upper lip, primary palate and medial edge epithelia of the secondary palate. At E14.5 expression could be seen in the medial edge epithelial seam.1 Publication

Inductioni

By hypoxia.1 Publication

Gene expression databases

BgeeiENSMUSG00000026021.
CleanExiMM_SUMO1.
ExpressionAtlasiP63166. baseline and differential.
GenevisibleiP63166. MM.

Interactioni

Subunit structurei

Interacts with USP25 (via ts SIM domain) the interaction weakly sumoylates USP25. Covalently attached to KCNB1; UBE2I increases cross-linking with KCNB1 and PIAS1 decreases cross-links with KCNB1 (By similarity). Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Interacts with PRKN. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. Interacts with SIMC1, CASP8AP2, RNF111 AND SOBP (via SIM domains). Interacts with BHLHE40/DEC1. Interacts with RWDD3. Interacts with UBE2I/UBC9 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36Interaction with PIAS2By similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204420. 22 interactors.
CORUMiP63166.
DIPiDIP-29278N.
IntActiP63166. 13 interactors.
MINTiMINT-154756.
STRINGi10090.ENSMUSP00000088935.

Structurei

3D structure databases

ProteinModelPortaliP63166.
SMRiP63166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 97Ubiquitin-likePROSITE-ProRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the ubiquitin family. SUMO subfamily.Curated

Phylogenomic databases

eggNOGiKOG1769. Eukaryota.
COG5227. LUCA.
GeneTreeiENSGT00390000018808.
HOGENOMiHOG000207495.
HOVERGENiHBG053025.
InParanoidiP63166.
KOiK12160.
OMAiTDNHTPK.
OrthoDBiEOG091G10ZQ.
PhylomeDBiP63166.
TreeFamiTF315116.

Family and domain databases

CDDicd01763. Sumo. 1 hit.
InterProiView protein in InterPro
IPR022617. Rad60/SUMO-like_dom.
IPR033950. Sumo.
IPR029071. Ubiquitin-like_domsf.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF11976. Rad60-SLD. 1 hit.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES
60 70 80 90 100
YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST

V
Length:101
Mass (Da):11,557
Last modified:September 27, 2004 - v1
Checksum:i89BE97D2D054FB33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033353 mRNA. Translation: AAC39959.1.
AK002536 mRNA. Translation: BAB22172.1.
AK011074 mRNA. Translation: BAB27379.1.
AK089081 mRNA. Translation: BAC40739.1.
AK159366 mRNA. Translation: BAE35024.1.
BC082566 mRNA. Translation: AAH82566.1.
BC083158 mRNA. Translation: AAH83158.1.
CCDSiCCDS35586.1.
RefSeqiNP_033486.1. NM_009460.2.
UniGeneiMm.362118.

Genome annotation databases

EnsembliENSMUST00000091374; ENSMUSP00000088935; ENSMUSG00000026021.
GeneIDi22218.
KEGGimmu:22218.
UCSCiuc007bdx.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiSUMO1_MOUSE
AccessioniPrimary (citable) accession number: P63166
Secondary accession number(s): P55856, Q3TX92, Q93068
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 22, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families