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Protein

Potassium voltage-gated channel subfamily E member 2

Gene

Kcne2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19219384). Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with HCN1 and HCN2 and increase potassium current (By similarity). Interacts with KCNQ1; forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity).By similarity1 Publication

GO - Molecular functioni

  • cation channel activity Source: RGD
  • delayed rectifier potassium channel activity Source: Ensembl
  • inward rectifier potassium channel activity Source: Ensembl
  • ion channel binding Source: UniProtKB
  • potassium channel regulator activity Source: UniProtKB
  • protein homodimerization activity Source: RGD
  • voltage-gated potassium channel activity Source: RGD
  • voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization Source: Ensembl

GO - Biological processi

  • aging Source: RGD
  • cellular response to drug Source: BHF-UCL
  • membrane repolarization Source: BHF-UCL
  • membrane repolarization during action potential Source: BHF-UCL
  • negative regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • negative regulation of voltage-gated potassium channel activity Source: RGD
  • positive regulation of proteasomal protein catabolic process Source: Ensembl
  • positive regulation of voltage-gated calcium channel activity Source: RGD
  • potassium ion export Source: BHF-UCL
  • potassium ion import Source: BHF-UCL
  • potassium ion transmembrane transport Source: BHF-UCL
  • regulation of cyclic nucleotide-gated ion channel activity Source: RGD
  • regulation of delayed rectifier potassium channel activity Source: BHF-UCL
  • regulation of heart rate by cardiac conduction Source: Ensembl
  • regulation of inward rectifier potassium channel activity Source: BHF-UCL
  • regulation of membrane repolarization Source: BHF-UCL
  • regulation of potassium ion transmembrane transport Source: BHF-UCL
  • regulation of ventricular cardiac muscle cell membrane repolarization Source: Ensembl
  • tongue development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-RNO-5576890. Phase 3 - rapid repolarisation.
R-RNO-5576893. Phase 2 - plateau phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily E member 2
Alternative name(s):
MinK-related peptide 1
Minimum potassium ion channel-related peptide 1
Potassium channel subunit beta MiRP1
Gene namesi
Name:Kcne2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi621383. Kcne2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 12354CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: Ensembl
  • integral component of membrane Source: RGD
  • lysosome Source: Ensembl
  • plasma membrane Source: UniProtKB
  • voltage-gated potassium channel complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 123123Potassium voltage-gated channel subfamily E member 2PRO_0000144288Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence analysis
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP63161.
PRIDEiP63161.

PTM databases

iPTMnetiP63161.
PhosphoSiteiP63161.

Expressioni

Tissue specificityi

Expressed in the heart (PubMed:19219384).1 Publication

Gene expression databases

GenevisibleiP63161. RN.

Interactioni

Subunit structurei

Interacts with KCNB1 (PubMed:19219384). Associates with KCNH2/ERG1 (By similarity). May associate with KCNQ2 and KCNQ3 (By similarity). Associates with HCN1 and probably HCN2 (PubMed:11420311). Heteromultimer with KCNC2. Interacts with KCNC2 (PubMed:14679187). Interacts with KCNQ1; forms a heterooligomer complex that targets to the membrane raft and leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity).By similarity3 Publications

GO - Molecular functioni

  • ion channel binding Source: UniProtKB
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045279.

Family & Domainsi

Sequence similaritiesi

Belongs to the potassium channel KCNE family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IX4Y. Eukaryota.
ENOG411297Y. LUCA.
GeneTreeiENSGT00510000048894.
HOGENOMiHOG000113208.
HOVERGENiHBG052227.
InParanoidiP63161.
KOiK04896.
OMAiNWRRNTT.
OrthoDBiEOG7WDN4J.
PhylomeDBiP63161.
TreeFamiTF336058.

Family and domain databases

InterProiIPR000369. K_chnl_KCNE.
IPR005425. K_chnl_volt-dep_bsu_KCNE2.
[Graphical view]
PfamiPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSiPR01605. KCNE2CHANNEL.

Sequencei

Sequence statusi: Complete.

P63161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLANLTQT LEDAFKKVFI TYMDSWRRNT TAEQQALQAR VDAENFYYVI
60 70 80 90 100
LYLMVMIGMF AFIVVAILVS TVKSKRREHS QDPYHQYIVE DWQQKYRSQI
110 120
LHLEDSKATI HENLGATGFT VSP
Length:123
Mass (Da):14,356
Last modified:September 27, 2004 - v1
Checksum:iCB91870E7B1EB82A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071003 mRNA. Translation: AAD28087.1.
BC089778 mRNA. Translation: AAH89778.1.
RefSeqiNP_598287.1. NM_133603.2.
XP_006248085.1. XM_006248023.2.
XP_008766776.1. XM_008768554.1.
UniGeneiRn.48691.

Genome annotation databases

EnsembliENSRNOT00000040844; ENSRNOP00000045279; ENSRNOG00000029811.
GeneIDi171138.
KEGGirno:171138.
UCSCiRGD:621383. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071003 mRNA. Translation: AAD28087.1.
BC089778 mRNA. Translation: AAH89778.1.
RefSeqiNP_598287.1. NM_133603.2.
XP_006248085.1. XM_006248023.2.
XP_008766776.1. XM_008768554.1.
UniGeneiRn.48691.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045279.

PTM databases

iPTMnetiP63161.
PhosphoSiteiP63161.

Proteomic databases

PaxDbiP63161.
PRIDEiP63161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040844; ENSRNOP00000045279; ENSRNOG00000029811.
GeneIDi171138.
KEGGirno:171138.
UCSCiRGD:621383. rat.

Organism-specific databases

CTDi9992.
RGDi621383. Kcne2.

Phylogenomic databases

eggNOGiENOG410IX4Y. Eukaryota.
ENOG411297Y. LUCA.
GeneTreeiENSGT00510000048894.
HOGENOMiHOG000113208.
HOVERGENiHBG052227.
InParanoidiP63161.
KOiK04896.
OMAiNWRRNTT.
OrthoDBiEOG7WDN4J.
PhylomeDBiP63161.
TreeFamiTF336058.

Enzyme and pathway databases

ReactomeiR-RNO-5576890. Phase 3 - rapid repolarisation.
R-RNO-5576893. Phase 2 - plateau phase.

Miscellaneous databases

PROiP63161.

Gene expression databases

GenevisibleiP63161. RN.

Family and domain databases

InterProiIPR000369. K_chnl_KCNE.
IPR005425. K_chnl_volt-dep_bsu_KCNE2.
[Graphical view]
PfamiPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSiPR01605. KCNE2CHANNEL.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MiRP1 forms IKr potassium channels with HERG and is associated with cardiac arrhythmia."
    Abbott G.W., Sesti F., Splawski I., Buck M.E., Lehmann M.H., Timothy K.W., Keating M.T., Goldstein S.A.N.
    Cell 97:175-187(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. "MinK-related peptide 1: a beta subunit for the HCN ion channel subunit family enhances expression and speeds activation."
    Yu H., Wu J., Potapova I., Wymore R.T., Holmes B., Zuckerman J., Pan Z., Wang H., Shi W., Robinson R.B., El-Maghrabi M.R., Benjamin W., Dixon J.E., McKinnon D., Cohen I.S., Wymore R.
    Circ. Res. 88:E84-E87(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HCN1.
  4. "MinK, MiRP1, and MiRP2 diversify Kv3.1 and Kv3.2 potassium channel gating."
    Lewis A., McCrossan Z.A., Abbott G.W.
    J. Biol. Chem. 279:7884-7892(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH KCNC2, SUBCELLULAR LOCATION.
  5. "Regulation of the Kv2.1 potassium channel by MinK and MiRP1."
    McCrossan Z.A., Roepke T.K., Lewis A., Panaghie G., Abbott G.W.
    J. Membr. Biol. 228:1-14(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KCNB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCNE2_RAT
AccessioniPrimary (citable) accession number: P63161
Secondary accession number(s): Q5FVT9, Q9WTW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.