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P63158 (HMGB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High mobility group protein B1
Alternative name(s):
High mobility group protein 1
Short name=HMG-1
Gene names
Name:Hmgb1
Synonyms:Hmg-1, Hmg1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Ref.7

Subunit structure

Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Ref.7

Subcellular location

Nucleus. Cytoplasm. Chromosome. Note: Associated with the plasma membrane of filipodia in process-growing cells, and also deposited into the substrate-attached material.

Sequence similarities

Belongs to the HMGB family.

Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
   Cellular componentChromosome
Cytoplasm
Nucleus
   DomainRepeat
   LigandDNA-binding
Heparin-binding
   PTMAcetylation
Isopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processV(D)J recombination

Inferred from electronic annotation. Source: Ensembl

base-excision repair, DNA ligation

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Traceable author statement PubMed 20826760. Source: BHF-UCL

inflammatory response to antigenic stimulus

Inferred from electronic annotation. Source: Ensembl

negative regulation of RNA polymerase II transcriptional preinitiation complex assembly

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic cell clearance

Inferred from direct assay PubMed 20826760. Source: BHF-UCL

positive regulation of DNA binding

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

condensed chromosome

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Traceable author statement PubMed 20826760. Source: BHF-UCL

extracellular space

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcriptional repressor complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphatidylserine binding

Traceable author statement PubMed 20826760. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 11099378PubMed 22842346. Source: IntAct

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Havcr2Q8VIM04EBI-6665811,EBI-6665112
POU5F1Q018603EBI-6665811,EBI-475687From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 215214High mobility group protein B1
PRO_0000048528

Regions

DNA binding9 – 7971HMG box 1
DNA binding95 – 16369HMG box 2
Compositional bias186 – 21530Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue301N6-acetyllysine Ref.8
Modified residue351Phosphoserine By similarity
Modified residue431N6-acetyllysine Ref.8
Modified residue901N6-acetyllysine Ref.8
Modified residue1001Phosphoserine By similarity
Modified residue1411N6-acetyllysine Ref.8
Cross-link112Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict1791E → V in AAA57042. Ref.4
Sequence conflict1901D → E in CAA56631. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P63158 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 8A868DE266D552B5

FASTA21524,894
        10         20         30         40         50         60 
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF 

        70         80         90        100        110        120 
EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL 

       130        140        150        160        170        180 
SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK 

       190        200        210 
SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a mouse cDNA encoding the nonhistone chromosomal high mobility group protein-1 (HMG1)."
Yotov W.V., St Arnaud R.
Nucleic Acids Res. 20:3516-3516(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H/He.
[2]"The mouse gene coding for high mobility group 1 protein (HMG1)."
Ferrari S., Ronfani L., Calogero S., Bianchi M.
J. Biol. Chem. 269:28803-28808(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
Tissue: Liver.
[3]"Molecular cloning, expression analysis, and chromosomal localization of mouse Hmg1-containing sequences."
Pauken C.M., Nagle D.L., Bucan M., Lo C.W.
Mamm. Genome 5:91-99(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The conserved lymphokine element-0 in the IL5 promoter binds to a high mobility group-1 protein."
Marrugo J., Marsh D.G., Ghosh B.
Mol. Immunol. 33:1119-1125(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AKR/J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 31-43 AND 113-127, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[7]"Stimulation of V(D)J cleavage by high mobility group proteins."
van Gent D.C., Hiom K., Paull T.T., Gellert M.
EMBO J. 16:2665-2670(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE RAG COMPLEX.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-30; LYS-43; LYS-90 AND LYS-141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11997 mRNA. Translation: CAA78042.1.
X80457 Genomic DNA. Translation: CAA56631.1.
U00431 mRNA. Translation: AAA20508.1.
L38477 mRNA. Translation: AAA57042.1.
BC006586 mRNA. Translation: AAH06586.1.
BC008565 mRNA. Translation: AAH08565.1.
BC083067 mRNA. Translation: AAH83067.1.
BC085090 mRNA. Translation: AAH85090.1.
CCDSCCDS19883.1.
PIRI48688.
RefSeqNP_034569.1. NM_010439.3.
XP_003945388.1. XM_003945339.2.
XP_006504870.1. XM_006504807.1.
UniGeneMm.207047.
Mm.313345.

3D structure databases

DisProtDP00384.
ProteinModelPortalP63158.
SMRP63158. Positions 5-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200322. 1 interaction.
IntActP63158. 7 interactions.
MINTMINT-225214.

Chemistry

ChEMBLCHEMBL2311237.

PTM databases

PhosphoSiteP63158.

Proteomic databases

MaxQBP63158.
PaxDbP63158.
PRIDEP63158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000085546; ENSMUSP00000082682; ENSMUSG00000066551.
ENSMUST00000093196; ENSMUSP00000106131; ENSMUSG00000066551.
ENSMUST00000110505; ENSMUSP00000106132; ENSMUSG00000066551.
GeneID100862258.
15289.
KEGGmmu:100862258.
mmu:15289.
UCSCuc009apb.2. mouse.

Organism-specific databases

CTD3146.
MGIMGI:96113. Hmgb1.

Phylogenomic databases

eggNOGCOG5648.
HOGENOMHOG000197861.
HOVERGENHBG009000.
InParanoidP63158.
KOK10802.
OMADMGKPPV.
OrthoDBEOG7WHHBQ.
PhylomeDBP63158.
TreeFamTF105371.

Gene expression databases

ArrayExpressP63158.
BgeeP63158.
CleanExMM_HMGB1.
GenevestigatorP63158.

Family and domain databases

Gene3D1.10.30.10. 2 hits.
InterProIPR009071. HMG_box_dom.
IPR017967. HMG_boxA_CS.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
PF09011. HMG_box_2. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 2 hits.
[Graphical view]
SUPFAMSSF47095. SSF47095. 2 hits.
PROSITEPS00353. HMG_BOX_1. 1 hit.
PS50118. HMG_BOX_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio287931.
PROP63158.
SOURCESearch...

Entry information

Entry nameHMGB1_MOUSE
AccessionPrimary (citable) accession number: P63158
Secondary accession number(s): P07155, P27109, P27428
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot