P63158 (HMGB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High mobility group protein B1

Alternative name(s):
High mobility group protein 1
Short name=HMG-1

Gene names

Name:	Hmgb1
Synonyms:	Hmg-1, Hmg1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Ref.7
Subunit structure	Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Ref.7
Subcellular location	Nucleus. Cytoplasm. Chromosome. Note: Associated with the plasma membrane of filipodia in process-growing cells, and also deposited into the substrate-attached material.
Sequence similarities	Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
Cellular component	Chromosome Cytoplasm Nucleus
Domain	Repeat
Ligand	DNA-binding Heparin-binding
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	V(D)J recombination Inferred from electronic annotation. Source: Compara base-excision repair, DNA ligation Inferred from electronic annotation. Source: Compara inflammatory response Traceable author statement PubMed 20826760. Source: BHF-UCL inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara negative regulation of RNA polymerase II transcriptional preinitiation complex assembly Inferred from electronic annotation. Source: Compara negative regulation of apoptotic cell clearance Inferred from direct assay PubMed 20826760. Source: BHF-UCL positive regulation of DNA binding Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara condensed chromosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Traceable author statement PubMed 20826760. Source: BHF-UCL transcriptional repressor complex Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW heparin binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylserine binding Traceable author statement PubMed 20826760. Source: BHF-UCL sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 215	214	High mobility group protein B1		PRO_0000048528
Regions
	DNA binding	9 – 79	71	HMG box 1
	DNA binding	95 – 163	69	HMG box 2
	Compositional bias	186 – 215	30	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	30	1	N6-acetyllysine By similarity
	Modified residue	35	1	Phosphoserine By similarity
	Modified residue	100	1	Phosphoserine By similarity
	Cross-link	112		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	179	1	E → V in AAA57042. Ref.4
	Sequence conflict	190	1	D → E in CAA56631. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P63158 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8A868DE266D552B5	FASTA	215	24,894
	10 20 30 40 50 60 MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF 70 80 90 100 110 120 EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL 130 140 150 160 170 180 SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK 190 200 210 SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a mouse cDNA encoding the nonhistone chromosomal high mobility group protein-1 (HMG1)." Yotov W.V., St Arnaud R. Nucleic Acids Res. 20:3516-3516(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/He.
[2]	"The mouse gene coding for high mobility group 1 protein (HMG1)." Ferrari S., Ronfani L., Calogero S., Bianchi M. J. Biol. Chem. 269:28803-28808(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Liver.
[3]	"Molecular cloning, expression analysis, and chromosomal localization of mouse Hmg1-containing sequences." Pauken C.M., Nagle D.L., Bucan M., Lo C.W. Mamm. Genome 5:91-99(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The conserved lymphokine element-0 in the IL5 promoter binds to a high mobility group-1 protein." Marrugo J., Marsh D.G., Ghosh B. Mol. Immunol. 33:1119-1125(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AKR/J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 31-43 AND 113-127, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[7]	"Stimulation of V(D)J cleavage by high mobility group proteins." van Gent D.C., Hiom K., Paull T.T., Gellert M. EMBO J. 16:2665-2670(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RAG COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11997 mRNA. Translation: CAA78042.1. X80457 Genomic DNA. Translation: CAA56631.1. U00431 mRNA. Translation: AAA20508.1. L38477 mRNA. Translation: AAA57042.1. BC006586 mRNA. Translation: AAH06586.1. BC008565 mRNA. Translation: AAH08565.1. BC083067 mRNA. Translation: AAH83067.1. BC085090 mRNA. Translation: AAH85090.1.
IPI	IPI00420261.
PIR	I48688.
RefSeq	NP_034569.1. NM_010439.3. XP_003945388.1. XM_003945339.1.
UniGene	Mm.207047. Mm.313345.
3D structure databases
DisProt	DP00384.
ProteinModelPortal	P63158.
SMR	P63158. Positions 5-166.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-225214.
PTM databases
PhosphoSite	P63158.
Proteomic databases
PaxDb	P63158.
PRIDE	P63158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000085546; ENSMUSP00000082682; ENSMUSG00000066551. ENSMUST00000093196; ENSMUSP00000106131; ENSMUSG00000066551. ENSMUST00000110505; ENSMUSP00000106132; ENSMUSG00000066551.
GeneID	100862258. 15289.
KEGG	mmu:100862258. mmu:15289.
Organism-specific databases
CTD	3146.
MGI	MGI:96113. Hmgb1.
Phylogenomic databases
eggNOG	COG5648.
HOGENOM	HOG000197861.
HOVERGEN	HBG009000.
InParanoid	P63158.
KO	K10802.
OMA	GEDAMRK.
OrthoDB	EOG4KWJV1.
Gene expression databases
ArrayExpress	P63158.
Bgee	P63158.
CleanEx	MM_HMGB1.
Genevestigator	P63158.
GermOnline	ENSMUSG00000057024. Mus musculus. ENSMUSG00000066551. Mus musculus.
Family and domain databases
Gene3D	1.10.30.10. 2 hits.
InterPro	IPR009071. HMG_box_dom. IPR017967. HMG_boxA_CS. [Graphical view]
Pfam	PF09011. DUF1898. 1 hit. PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 2 hits. [Graphical view]
SUPFAM	SSF47095. HMG-box. 2 hits.
PROSITE	PS00353. HMG_BOX_1. 1 hit. PS50118. HMG_BOX_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	287931.
SOURCE	Search...

Entry information

Entry name

HMGB1_MOUSE

Accession

Primary (citable) accession number: P63158
Secondary accession number(s): P07155, P27109, P27428

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program