P63151 (2ABA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform

Alternative name(s):
PP2A subunit B isoform B55-alpha
PP2A subunit B isoform PR55-alpha
PP2A subunit B isoform R2-alpha
PP2A subunit B isoform alpha

Gene names

Name:

PPP2R2A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD By similarity. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Ref.5
Tissue specificity	Expressed in all tissues examined. Ref.1
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA metabolic process Traceable author statement. Source: Reactome protein dephosphorylation Inferred from direct assay Ref.1. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Traceable author statement. Source: Reactome protein phosphatase type 2A complex Inferred from direct assay Ref.5Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: UniProtKB protein phosphatase type 2A regulator activity Traceable author statement Ref.1. Source: UniProtKB protein serine/threonine phosphatase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPP2R1B	P30154	2	EBI-1048931,EBI-357094

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 447

446

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform

PRO_0000071415

Regions

Repeat

26 – 65

40

WD 1

Repeat

91 – 132

42

WD 2

Repeat

175 – 213

39

WD 3

Repeat

224 – 264

41

WD 4

Repeat

283 – 321

39

WD 5

Repeat

338 – 379

42

WD 6

Repeat

414 – 446

33

WD 7

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.6

Secondary structure

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447

Helix Strand Turn

Details...

Beta strand

13 – 16

4

Helix

27 – 29

3

Beta strand

42 – 45

4

Beta strand

52 – 57

6

Beta strand

71 – 77

7

Beta strand

83 – 85

3

Helix

86 – 88

3

Beta strand

90 – 92

3

Beta strand

98 – 101

4

Beta strand

106 – 114

9

Beta strand

119 – 132

14

Beta strand

156 – 172

17

Beta strand

182 – 185

4

Beta strand

189 – 195

7

Beta strand

197 – 204

8

Beta strand

207 – 216

10

Helix

222 – 224

3

Beta strand

229 – 234

6

Beta strand

241 – 246

6

Beta strand

251 – 255

5

Turn

256 – 258

3

Beta strand

260 – 262

3

Beta strand

267 – 269

3

Helix

280 – 284

5

Beta strand

288 – 293

6

Beta strand

300 – 312

13

Beta strand

323 – 325

3

Helix

327 – 329

3

Turn

330 – 332

3

Helix

333 – 338

6

Helix

341 – 343

3

Beta strand

355 – 359

5

Beta strand

365 – 370

6

Turn

371 – 373

3

Beta strand

376 – 380

5

Helix

410 – 412

3

Beta strand

421 – 424

4

Beta strand

426 – 434

9

Beta strand

439 – 443

5

Sequences

	Sequence		Length	Mass (Da)
	P63151 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: F4D407FF7ADA4ED6	FASTA	447	51,692
	10 20 30 40 50 60 MAGAGGGNDI QWCFSQVKGA VDDDVAEADI ISTVEFNHSG ELLATGDKGG RVVIFQQEQE 70 80 90 100 110 120 NKIQSHSRGE YNVYSTFQSH EPEFDYLKSL EIEEKINKIR WLPQKNAAQF LLSTNDKTIK 130 140 150 160 170 180 LWKISERDKR PEGYNLKEED GRYRDPTTVT TLRVPVFRPM DLMVEASPRR IFANAHTYHI 190 200 210 220 230 240 NSISINSDYE TYLSADDLRI NLWHLEITDR SFNIVDIKPA NMEELTEVIT AAEFHPNSCN 250 260 270 280 290 300 TFVYSSSKGT IRLCDMRASA LCDRHSKLFE EPEDPSNRSF FSEIISSISD VKFSHSGRYM 310 320 330 340 350 360 MTRDYLSVKI WDLNMENRPV ETYQVHEYLR SKLCSLYEND CIFDKFECCW NGSDSVVMTG 370 380 390 400 410 420 SYNNFFRMFD RNTKRDITLE ASRENNKPRT VLKPRKVCAS GKRKKDEISV DSLDFNKKIL 430 440 HTAWHPKENI IAVATTNNLY IFQDKVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal-specific isoform." Mayer R.E., Hendrix P., Cron P., Matthies R., Stone S.R., Goris J., Merlevede W., Hofsteenge J., Hemmings B.A. Biochemistry 30:3589-3597(1991) [PubMed: 1849734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Lung fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[5]	"A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55." Li H.H., Cai X., Shouse G.P., Piluso L.G., Liu X. EMBO J. 26:402-411(2007) [PubMed: 17245430] [Abstract] Cited for: INTERACTION WITH TP53.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M64929 mRNA. Translation: AAA36490.1.
AK314823 mRNA. Translation: BAG37345.1.
CH471080 Genomic DNA. Translation: EAW63578.1.
BC041071 mRNA. Translation: AAH41071.1.

IPI

IPI00332511.

PIR

A38351.

RefSeq

NP_001171062.1. NM_001177591.1.
NP_002708.1. NM_002717.3.

UniGene

Hs.146339.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DW8	X-ray	2.85	B/E	1-447	[»]

ProteinModelPortal

P63151.

SMR

P63151. Positions 8-446.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29398N.

IntAct

P63151. 7 interactions.

MINT

MINT-2835351.

STRING

P63151.

PTM databases

PhosphoSite

P63151.

Polymorphism databases

DMDM

52783535.

Proteomic databases

PeptideAtlas

P63151.

PRIDE

P63151.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000380737; ENSP00000370113; ENSG00000221914.

GeneID

5520.

KEGG

hsa:5520.

UCSC

uc003xeu.1. human.

Organism-specific databases

CTD

5520.

GeneCards

GC08P026204.

H-InvDB

HIX0007394.

HGNC

HGNC:9304. PPP2R2A.

MIM

604941. gene.

neXtProt

NX_P63151.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG000012.

InParanoid

P63151.

OrthoDB

EOG4M0F1K.

PhylomeDB

P63151.

Enzyme and pathway databases

Pathway_Interaction_DB

tgfbrpathway. TGF-beta receptor signaling.

Reactome

REACT_111217. Metabolism.
REACT_152. Cell Cycle, Mitotic.

Gene expression databases

ArrayExpress

P63151.

Bgee

P63151.

CleanEx

HS_PPP2R2A.

Genevestigator

P63151.

GermOnline

ENSG00000147459. Homo sapiens.

Family and domain databases

InterPro

IPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 3 hits.

KO

K04354.

PANTHER

PTHR11871. Pp2A_PR55. 1 hit.

Pfam

PF00400. WD40. 2 hits.
[Graphical view]

PIRSF

PIRSF037309. PP2A_PR55. 1 hit.

PRINTS

PR00600. PP2APR55.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

21352.

SOURCE

Search...

Entry information

Entry name

2ABA_HUMAN

Accession

Primary (citable) accession number: P63151
Secondary accession number(s): B2RBU8, P50409, Q00007

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.