Reviewed, UniProtKB/Swiss-Prot P63151 (2ABA_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
      Short name=PP2A, subunit B, B-alpha isoform
Alternative name(s):
    PP2A, subunit B, B55-alpha isoform
    PP2A, subunit B, PR55-alpha isoform
    PP2A, subunit B, R2-alpha isoform

Gene names

Name:

PPP2R2A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Tissue specificity	In all tissues examined. Ref.1
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Ref.1 Inferred from direct assay. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	protein phosphatase type 2A complex Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB protein phosphatase type 2A regulator activity Ref.1 Traceable author statement. Source: UniProtKB protein serine/threonine phosphatase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FAM107A	O95990	1	EBI-1048931,EBI-743396
NDRG1	Q92597	1	EBI-1048931,EBI-716486
PPP2R1B	P30154	1	EBI-1048931,EBI-357094

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform		PRO_0000071415
Regions
	Repeat	26 – 65	40	WD 1
	Repeat	91 – 132	42	WD 2
	Repeat	175 – 213	39	WD 3
	Repeat	224 – 264	41	WD 4
	Repeat	283 – 321	39	WD 5
	Repeat	338 – 379	42	WD 6
	Repeat	414 – 446	33	WD 7

Sequences

	Sequence		Length	Mass (Da)
	P63151-1 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: F4D407FF7ADA4ED6	FASTA	447	51,692
	10 20 30 40 50 60 MAGAGGGNDI QWCFSQVKGA VDDDVAEADI ISTVEFNHSG ELLATGDKGG RVVIFQQEQE 70 80 90 100 110 120 NKIQSHSRGE YNVYSTFQSH EPEFDYLKSL EIEEKINKIR WLPQKNAAQF LLSTNDKTIK 130 140 150 160 170 180 LWKISERDKR PEGYNLKEED GRYRDPTTVT TLRVPVFRPM DLMVEASPRR IFANAHTYHI 190 200 210 220 230 240 NSISINSDYE TYLSADDLRI NLWHLEITDR SFNIVDIKPA NMEELTEVIT AAEFHPNSCN 250 260 270 280 290 300 TFVYSSSKGT IRLCDMRASA LCDRHSKLFE EPEDPSNRSF FSEIISSISD VKFSHSGRYM 310 320 330 340 350 360 MTRDYLSVKI WDLNMENRPV ETYQVHEYLR SKLCSLYEND CIFDKFECCW NGSDSVVMTG 370 380 390 400 410 420 SYNNFFRMFD RNTKRDITLE ASRENNKPRT VLKPRKVCAS GKRKKDEISV DSLDFNKKIL 430 440 HTAWHPKENI IAVATTNNLY IFQDKVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal-specific isoform." Mayer R.E., Hendrix P., Cron P., Matthies R., Stone S.R., Goris J., Merlevede W., Hofsteenge J., Hemmings B.A. Biochemistry 30:3589-3597(1991) [PubMed: 1849734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Lung fibroblast.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M64929 mRNA. Translation: AAA36490.1.
BC041071 mRNA. Translation: AAH41071.1.

IPI

IPI00332511.

PIR

A38351.

RefSeq

NP_002708.1.

UniGene

Hs.146339

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DW8	X-ray	2.85	B/E	1-447	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P63151. 8 interactions.

Proteomic databases

PeptideAtlas

P63151.

PRIDE

P63151.

Genome annotation databases

Ensembl

ENSG00000221914. Homo sapiens. [Contig view]

GeneID

5520.

KEGG

hsa:5520.

Organism-specific databases

GeneCards

GC08P026204.

H-InvDB

HIX0007394.

HGNC

HGNC:9304. PPP2R2A.

MIM

604941. gene.

PharmGKB

PA33668.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P63151.

Enzyme and pathway databases

Pathway_Interaction_DB

tgfbrpathway. TGF-beta receptor signaling.

Gene expression databases

ArrayExpress

P63151.

Bgee

P63151.

CleanEx

HS_PPP2R2A.

GermOnline

ENSG00000147459. Homo sapiens.

Family and domain databases

InterPro

IPR019578. PP2A_Bsu_A/B_central-region.
IPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR019573. PP2A_suB_N.
IPR015943. WD40/YVTN_repeat-like.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

PANTHER

PTHR11871. Pp2A_PR55. 1 hit.

Pfam

PF10586. PP2A_B_N. 1 hit.
PF10594. PP2A_B_subs_rcg. 1 hit.
[Graphical view]

PIRSF

PIRSF037309. PP2A_PR55. 1 hit.

PRINTS

PR00600. PP2APR55.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

PROSITE

PS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

21352.

SOURCE

Search...

Entry information

Entry name

2ABA_HUMAN

Accession

Primary (citable) accession number: P63151
Secondary accession number(s): P50409, Q00007

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)