P63151 (2ABA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform

Alternative name(s):
PP2A subunit B isoform B55-alpha
PP2A subunit B isoform PR55-alpha
PP2A subunit B isoform R2-alpha
PP2A subunit B isoform alpha

Gene names

Name:

PPP2R2A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD By similarity. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Ref.6
Tissue specificity	Expressed in all tissues examined. Ref.1
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gene expression Traceable author statement. Source: Reactome nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: Reactome protein dephosphorylation Inferred from direct assay Ref.1. Source: UniProtKB response to morphine Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Traceable author statement. Source: Reactome protein phosphatase type 2A complex Inferred from direct assay Ref.6Ref.1. Source: UniProtKB
Molecular_function	protein phosphatase type 2A regulator activity Traceable author statement Ref.1. Source: UniProtKB protein serine/threonine phosphatase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPP2R1B	P30154	2	EBI-1048931,EBI-357094

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P63151-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P63151-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MA → MFPKFSLRSMFH

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 447

447

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform

PRO_0000071415

Regions

Repeat

26 – 65

40

WD 1

Repeat

91 – 132

42

WD 2

Repeat

175 – 213

39

WD 3

Repeat

224 – 264

41

WD 4

Repeat

283 – 321

39

WD 5

Repeat

338 – 379

42

WD 6

Repeat

414 – 446

33

WD 7

Natural variations

Alternative sequence

1 – 2

2

MA → MFPKFSLRSMFH in isoform 2.

VSP_043100

Secondary structure

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447

Helix Strand Turn

Details...

Beta strand

13 – 16

4

Beta strand

21 – 23

3

Helix

27 – 29

3

Beta strand

30 – 36

7

Beta strand

38 – 47

10

Beta strand

50 – 57

8

Beta strand

71 – 78

8

Beta strand

83 – 85

3

Helix

86 – 88

3

Beta strand

90 – 92

3

Beta strand

98 – 101

4

Beta strand

106 – 114

9

Beta strand

119 – 132

14

Beta strand

156 – 172

17

Beta strand

182 – 185

4

Beta strand

189 – 195

7

Beta strand

197 – 204

8

Beta strand

207 – 216

10

Helix

222 – 224

3

Beta strand

229 – 234

6

Beta strand

241 – 246

6

Beta strand

251 – 255

5

Turn

256 – 258

3

Beta strand

260 – 262

3

Beta strand

267 – 269

3

Helix

280 – 284

5

Beta strand

288 – 293

6

Beta strand

297 – 312

16

Beta strand

323 – 325

3

Helix

327 – 329

3

Turn

330 – 332

3

Helix

333 – 338

6

Helix

341 – 343

3

Beta strand

348 – 350

3

Beta strand

354 – 360

7

Beta strand

365 – 370

6

Turn

371 – 373

3

Beta strand

376 – 380

5

Helix

410 – 412

3

Beta strand

421 – 424

4

Beta strand

426 – 434

9

Beta strand

439 – 443

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: F4D407FF7ADA4ED6	FASTA	447	51,692
	10 20 30 40 50 60 MAGAGGGNDI QWCFSQVKGA VDDDVAEADI ISTVEFNHSG ELLATGDKGG RVVIFQQEQE 70 80 90 100 110 120 NKIQSHSRGE YNVYSTFQSH EPEFDYLKSL EIEEKINKIR WLPQKNAAQF LLSTNDKTIK 130 140 150 160 170 180 LWKISERDKR PEGYNLKEED GRYRDPTTVT TLRVPVFRPM DLMVEASPRR IFANAHTYHI 190 200 210 220 230 240 NSISINSDYE TYLSADDLRI NLWHLEITDR SFNIVDIKPA NMEELTEVIT AAEFHPNSCN 250 260 270 280 290 300 TFVYSSSKGT IRLCDMRASA LCDRHSKLFE EPEDPSNRSF FSEIISSISD VKFSHSGRYM 310 320 330 340 350 360 MTRDYLSVKI WDLNMENRPV ETYQVHEYLR SKLCSLYEND CIFDKFECCW NGSDSVVMTG 370 380 390 400 410 420 SYNNFFRMFD RNTKRDITLE ASRENNKPRT VLKPRKVCAS GKRKKDEISV DSLDFNKKIL 430 440 HTAWHPKENI IAVATTNNLY IFQDKVN
	Isoform 2 [UniParc]. Checksum: 242C50314171EDDE	FASTA	457	53,000
	10 20 30 40 50 60 MFPKFSLRSM FHGAGGGNDI QWCFSQVKGA VDDDVAEADI ISTVEFNHSG ELLATGDKGG 70 80 90 100 110 120 RVVIFQQEQE NKIQSHSRGE YNVYSTFQSH EPEFDYLKSL EIEEKINKIR WLPQKNAAQF 130 140 150 160 170 180 LLSTNDKTIK LWKISERDKR PEGYNLKEED GRYRDPTTVT TLRVPVFRPM DLMVEASPRR 190 200 210 220 230 240 IFANAHTYHI NSISINSDYE TYLSADDLRI NLWHLEITDR SFNIVDIKPA NMEELTEVIT 250 260 270 280 290 300 AAEFHPNSCN TFVYSSSKGT IRLCDMRASA LCDRHSKLFE EPEDPSNRSF FSEIISSISD 310 320 330 340 350 360 VKFSHSGRYM MTRDYLSVKI WDLNMENRPV ETYQVHEYLR SKLCSLYEND CIFDKFECCW 370 380 390 400 410 420 NGSDSVVMTG SYNNFFRMFD RNTKRDITLE ASRENNKPRT VLKPRKVCAS GKRKKDEISV 430 440 450 DSLDFNKKIL HTAWHPKENI IAVATTNNLY IFQDKVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal-specific isoform." Mayer R.E., Hendrix P., Cron P., Matthies R., Stone S.R., Goris J., Merlevede W., Hofsteenge J., Hemmings B.A. Biochemistry 30:3589-3597(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Lung fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Trachea.
[3]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Blood.
[6]	"A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55." Li H.H., Cai X., Shouse G.P., Piluso L.G., Liu X. EMBO J. 26:402-411(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M64929 mRNA. Translation: AAA36490.1.
AK303981 mRNA. Translation: BAG64899.1.
AK314823 mRNA. Translation: BAG37345.1.
AC022911 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63578.1.
BC041071 mRNA. Translation: AAH41071.1.

IPI

IPI00332511.
IPI00798041.

PIR

A38351.

RefSeq

NP_001171062.1. NM_001177591.1.
NP_002708.1. NM_002717.3.

UniGene

Hs.146339.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DW8	X-ray	2.85	B/E	1-447	[»]

ProteinModelPortal

P63151.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29398N.

IntAct

P63151. 8 interactions.

MINT

MINT-2835351.

STRING

9606.ENSP00000370113.

PTM databases

PhosphoSite

P63151.

Polymorphism databases

DMDM

52783535.

Proteomic databases

PaxDb

P63151.

PeptideAtlas

P63151.

PRIDE

P63151.

Protocols and materials databases

DNASU

5520.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000315985; ENSP00000325074; ENSG00000221914.
ENST00000380737; ENSP00000370113; ENSG00000221914.

GeneID

5520.

KEGG

hsa:5520.

UCSC

uc003xeu.3. human.

Organism-specific databases

CTD

5520.

GeneCards

GC08P026204.

HGNC

HGNC:9304. PPP2R2A.

MIM

604941. gene.

neXtProt

NX_P63151.

PharmGKB

PA33668.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5170.

HOGENOM

HOG000089745.

HOVERGEN

HBG000012.

InParanoid

P63151.

KO

K04354.

OMA

EFHPTEC.

OrthoDB

EOG4M0F1K.

PhylomeDB

P63151.

Enzyme and pathway databases

Pathway_Interaction_DB

tgfbrpathway. TGF-beta receptor signaling.

Reactome

REACT_115566. Cell Cycle.
REACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P63151.

Bgee

P63151.

CleanEx

HS_PPP2R2A.

Genevestigator

P63151.

GermOnline

ENSG00000147459. Homo sapiens.

Family and domain databases

Gene3D

2.130.10.10. 3 hits.

InterPro

IPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]

PANTHER

PTHR11871. PTHR11871. 1 hit.

PIRSF

PIRSF037309. PP2A_PR55. 1 hit.

PRINTS

PR00600. PP2APR55.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P63151.

ChEMBL

CHEMBL4284.

ChiTaRS

PPP2R2A. human.

EvolutionaryTrace

P63151.

GenomeRNAi

5520.

NextBio

21352.

SOURCE

Search...

Entry information

Entry name

2ABA_HUMAN

Accession

Primary (citable) accession number: P63151
Secondary accession number(s): B2RBU8 , B4E1T7, P50409, Q00007

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.