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Protein

Ubiquitin-conjugating enzyme E2 B

Gene

Ube2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation (By similarity). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.By similarity2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. small conjugating protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central
  6. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. canonical Wnt signaling pathway Source: Ensembl
  2. cellular response to insulin stimulus Source: RGD
  3. chiasma assembly Source: Ensembl
  4. DNA repair Source: GO_Central
  5. histone H2A ubiquitination Source: Ensembl
  6. histone lysine demethylation Source: Ensembl
  7. histone ubiquitination Source: GO_Central
  8. in utero embryonic development Source: Ensembl
  9. maintenance of chromatin silencing Source: Ensembl
  10. negative regulation of apoptotic process Source: Ensembl
  11. negative regulation of cAMP-mediated signaling Source: Ensembl
  12. negative regulation of histone phosphorylation Source: Ensembl
  13. positive regulation of reciprocal meiotic recombination Source: Ensembl
  14. postreplication repair Source: Ensembl
  15. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  16. protein autoubiquitination Source: Ensembl
  17. protein K11-linked ubiquitination Source: UniProtKB
  18. protein K48-linked ubiquitination Source: UniProtKB
  19. protein K63-linked ubiquitination Source: UniProtKB
  20. protein monoubiquitination Source: Ensembl
  21. protein polyubiquitination Source: GO_Central
  22. protein stabilization Source: Ensembl
  23. response to drug Source: Ensembl
  24. response to hypoxia Source: RGD
  25. response to UV Source: Ensembl
  26. sperm axoneme assembly Source: Ensembl
  27. synaptonemal complex organization Source: Ensembl
  28. ubiquitin-dependent protein catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
Alternative name(s):
RAD6 homolog B
Short name:
HR6B
Ubiquitin carrier protein B
Ubiquitin-conjugating enzyme E2(14k)
Ubiquitin-protein ligase B
Gene namesi
Name:Ube2b
Synonyms:Rad6b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi708345. Ube2b.

Subcellular locationi

Cell membrane 1 Publication. Nucleus 1 Publication
Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. HULC complex Source: UniProtKB
  3. nuclear chromatin Source: GO_Central
  4. plasma membrane Source: UniProtKB-SubCell
  5. replication fork Source: Ensembl
  6. XY body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 BPRO_0000082450Add
BLAST

Expressioni

Tissue specificityi

Expressed at high levels in testes, moderate levels in muscle, heart, and brain, and low levels in liver and kidney. Upon fasting, increased levels were seen in muscle, heart, liver, and kidney.1 Publication

Inductioni

Up-regulated by NGF.1 Publication

Gene expression databases

GenevestigatoriP63149.

Interactioni

Subunit structurei

Interacts with RAD18, UBR2 and WAC.By similarity

Protein-protein interaction databases

BioGridi249674. 1 interaction.
STRINGi10116.ENSRNOP00000016742.

Structurei

3D structure databases

ProteinModelPortaliP63149.
SMRiP63149. Positions 3-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP63149.
KOiK10574.
OMAiIVEQSWV.
OrthoDBiEOG7M0NTH.
PhylomeDBiP63149.
TreeFamiTF101128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63149-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED
60 70 80 90 100
GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN

DS
Length:152
Mass (Da):17,312
Last modified:September 27, 2004 - v1
Checksum:iCFDEEEE7E06840BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62388 mRNA. Translation: AAA21087.1.
U04308
, U04303, U04304, U04305, U04306, U04307 Genomic DNA. Translation: AAB60669.1.
AF144083 mRNA. Translation: AAD37966.1.
BC070946 mRNA. Translation: AAH70946.1.
PIRiI51913.
RefSeqiNP_112400.1. NM_031138.2.
UniGeneiRn.20766.

Genome annotation databases

EnsembliENSRNOT00000016742; ENSRNOP00000016742; ENSRNOG00000005064.
GeneIDi81816.
KEGGirno:81816.
UCSCiRGD:708345. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62388 mRNA. Translation: AAA21087.1.
U04308
, U04303, U04304, U04305, U04306, U04307 Genomic DNA. Translation: AAB60669.1.
AF144083 mRNA. Translation: AAD37966.1.
BC070946 mRNA. Translation: AAH70946.1.
PIRiI51913.
RefSeqiNP_112400.1. NM_031138.2.
UniGeneiRn.20766.

3D structure databases

ProteinModelPortaliP63149.
SMRiP63149. Positions 3-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249674. 1 interaction.
STRINGi10116.ENSRNOP00000016742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016742; ENSRNOP00000016742; ENSRNOG00000005064.
GeneIDi81816.
KEGGirno:81816.
UCSCiRGD:708345. rat.

Organism-specific databases

CTDi7320.
RGDi708345. Ube2b.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP63149.
KOiK10574.
OMAiIVEQSWV.
OrthoDBiEOG7M0NTH.
PhylomeDBiP63149.
TreeFamiTF101128.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi615721.
PROiP63149.

Gene expression databases

GenevestigatoriP63149.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6."
    Wing S.S., Dumas F., Banville D.
    J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and regulation upon fasting and by insulin."
    Wing S.S., Banville D.
    Am. J. Physiol. 267:E39-E48(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
  3. "Isolation of a ubiquitin-conjugating enzyme gene induced following cerebral ischemia from the rat brain using modified subtractive hybridization and differential screening."
    Chen D., Graham S.H., Jing K., Simon R.P., Chen J.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  5. "The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by nerve growth factor and required for neurite outgrowth."
    Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L.
    Mol. Cell. Neurosci. 29:559-568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiUBE2B_RAT
AccessioniPrimary (citable) accession number: P63149
Secondary accession number(s): P23567, Q9D0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: February 4, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.