P63149 (UBE2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 B
EC=6.3.2.19

Alternative name(s):
RAD6 homolog B
Short name=HR6B
Ubiquitin carrier protein B
Ubiquitin-conjugating enzyme E2(14k)
Ubiquitin-protein ligase B

Gene names

Name:	Ube2b
Synonyms:	Rad6b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation By similarity. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. Ref.1 Ref.5
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RAD18, UBR2 and WAC By similarity.
Subcellular location	Cell membrane. Nucleus. Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei. Ref.5
Tissue specificity	Expressed at high levels in testes, moderate levels in muscle, heart, and brain, and low levels in liver and kidney. Upon fasting, increased levels were seen in muscle, heart, liver, and kidney. Ref.2
Induction	Up-regulated by NGF. Ref.5
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Cell membrane Membrane Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara cellular response to insulin stimulus Inferred from expression pattern Ref.2. Source: RGD chiasma assembly Inferred from electronic annotation. Source: Compara histone H2A ubiquitination Inferred from electronic annotation. Source: Compara histone lysine demethylation Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from electronic annotation. Source: Compara maintenance of chromatin silencing Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of cAMP-mediated signaling Inferred from electronic annotation. Source: Compara negative regulation of histone phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of reciprocal meiotic recombination Inferred from electronic annotation. Source: Compara postreplication repair Inferred from electronic annotation. Source: Compara proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein autoubiquitination Inferred from electronic annotation. Source: Compara protein monoubiquitination Inferred from electronic annotation. Source: Compara protein stabilization Inferred from electronic annotation. Source: Compara response to UV Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from expression pattern PubMed 16483544. Source: RGD sperm axoneme assembly Inferred from electronic annotation. Source: Compara synaptonemal complex organization Inferred from electronic annotation. Source: Compara ubiquitin-dependent protein catabolic process Inferred from expression pattern Ref.2. Source: RGD
Cellular_component	HULC complex Inferred from sequence or structural similarity. Source: UniProtKB XY body Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara nuclear chromatin Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell replication fork Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Ubiquitin-conjugating enzyme E2 B		PRO_0000082450
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63149 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: CFDEEEE7E06840BE	FASTA	152	17,312
	10 20 30 40 50 60 MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS 70 80 90 100 110 120 EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 130 140 150 PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6." Wing S.S., Dumas F., Banville D. J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and regulation upon fasting and by insulin." Wing S.S., Banville D. Am. J. Physiol. 267:E39-E48(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
[3]	"Isolation of a ubiquitin-conjugating enzyme gene induced following cerebral ischemia from the rat brain using modified subtractive hybridization and differential screening." Chen D., Graham S.H., Jing K., Simon R.P., Chen J. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[5]	"The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by nerve growth factor and required for neurite outgrowth." Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L. Mol. Cell. Neurosci. 29:559-568(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62388 mRNA. Translation: AAA21087.1. U04308 , U04303, U04304, U04305, U04306, U04307 Genomic DNA. Translation: AAB60669.1. AF144083 mRNA. Translation: AAD37966.1. BC070946 mRNA. Translation: AAH70946.1.
IPI	IPI00211940.
PIR	I51913.
RefSeq	NP_112400.1. NM_031138.2.
UniGene	Rn.20766.
3D structure databases
ProteinModelPortal	P63149.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000016742.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016742; ENSRNOP00000016742; ENSRNOG00000005064.
GeneID	81816.
KEGG	rno:81816.
UCSC	RGD:708345. rat.
Organism-specific databases
CTD	7320.
RGD	708345. Ube2b.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00680000099547.
HOGENOM	HOG000233454.
HOVERGEN	HBG063308.
InParanoid	P63149.
KO	K10574.
OMA	VIFGPVG.
OrthoDB	EOG4RJG2S.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	P63149.
GermOnline	ENSRNOG00000005064. Rattus norvegicus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615721.

Entry information

Entry name

UBE2B_RAT

Accession

Primary (citable) accession number: P63149
Secondary accession number(s): P23567, Q9D0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program