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P63149

- UBE2B_RAT

UniProt

P63149 - UBE2B_RAT

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Protein
Ubiquitin-conjugating enzyme E2 B
Gene
Ube2b, Rad6b
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation By similarity. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. canonical Wnt signaling pathway Source: Ensembl
  2. cellular response to insulin stimulus Source: RGD
  3. chiasma assembly Source: Ensembl
  4. histone H2A ubiquitination Source: Ensembl
  5. histone lysine demethylation Source: Ensembl
  6. in utero embryonic development Source: Ensembl
  7. maintenance of chromatin silencing Source: Ensembl
  8. negative regulation of apoptotic process Source: Ensembl
  9. negative regulation of cAMP-mediated signaling Source: Ensembl
  10. negative regulation of histone phosphorylation Source: Ensembl
  11. positive regulation of reciprocal meiotic recombination Source: Ensembl
  12. postreplication repair Source: Ensembl
  13. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  14. protein K11-linked ubiquitination Source: UniProtKB
  15. protein K48-linked ubiquitination Source: UniProtKB
  16. protein K63-linked ubiquitination Source: UniProtKB
  17. protein autoubiquitination Source: Ensembl
  18. protein monoubiquitination Source: Ensembl
  19. protein stabilization Source: Ensembl
  20. response to UV Source: Ensembl
  21. response to drug Source: Ensembl
  22. response to hypoxia Source: RGD
  23. sperm axoneme assembly Source: Ensembl
  24. synaptonemal complex organization Source: Ensembl
  25. ubiquitin-dependent protein catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
Alternative name(s):
RAD6 homolog B
Short name:
HR6B
Ubiquitin carrier protein B
Ubiquitin-conjugating enzyme E2(14k)
Ubiquitin-protein ligase B
Gene namesi
Name:Ube2b
Synonyms:Rad6b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi708345. Ube2b.

Subcellular locationi

Cell membrane. Nucleus
Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.1 Publication

GO - Cellular componenti

  1. HULC complex Source: UniProtKB
  2. XY body Source: Ensembl
  3. cytoplasm Source: Ensembl
  4. nuclear chromatin Source: Ensembl
  5. plasma membrane Source: UniProtKB-SubCell
  6. replication fork Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 B
PRO_0000082450Add
BLAST

Expressioni

Tissue specificityi

Expressed at high levels in testes, moderate levels in muscle, heart, and brain, and low levels in liver and kidney. Upon fasting, increased levels were seen in muscle, heart, liver, and kidney.1 Publication

Inductioni

Up-regulated by NGF.1 Publication

Gene expression databases

GenevestigatoriP63149.

Interactioni

Subunit structurei

Interacts with RAD18, UBR2 and WAC By similarity.

Protein-protein interaction databases

BioGridi249674. 1 interaction.
STRINGi10116.ENSRNOP00000016742.

Structurei

3D structure databases

ProteinModelPortaliP63149.
SMRiP63149. Positions 3-152.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP63149.
KOiK10574.
OMAiIVEQSWV.
OrthoDBiEOG7M0NTH.
PhylomeDBiP63149.
TreeFamiTF101128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63149-1 [UniParc]FASTAAdd to Basket

« Hide

MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED    50
GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY 100
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN 150
DS 152
Length:152
Mass (Da):17,312
Last modified:September 27, 2004 - v1
Checksum:iCFDEEEE7E06840BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62388 mRNA. Translation: AAA21087.1.
U04308
, U04303, U04304, U04305, U04306, U04307 Genomic DNA. Translation: AAB60669.1.
AF144083 mRNA. Translation: AAD37966.1.
BC070946 mRNA. Translation: AAH70946.1.
PIRiI51913.
RefSeqiNP_112400.1. NM_031138.2.
UniGeneiRn.20766.

Genome annotation databases

EnsembliENSRNOT00000016742; ENSRNOP00000016742; ENSRNOG00000005064.
GeneIDi81816.
KEGGirno:81816.
UCSCiRGD:708345. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62388 mRNA. Translation: AAA21087.1 .
U04308
, U04303 , U04304 , U04305 , U04306 , U04307 Genomic DNA. Translation: AAB60669.1 .
AF144083 mRNA. Translation: AAD37966.1 .
BC070946 mRNA. Translation: AAH70946.1 .
PIRi I51913.
RefSeqi NP_112400.1. NM_031138.2.
UniGenei Rn.20766.

3D structure databases

ProteinModelPortali P63149.
SMRi P63149. Positions 3-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249674. 1 interaction.
STRINGi 10116.ENSRNOP00000016742.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000016742 ; ENSRNOP00000016742 ; ENSRNOG00000005064 .
GeneIDi 81816.
KEGGi rno:81816.
UCSCi RGD:708345. rat.

Organism-specific databases

CTDi 7320.
RGDi 708345. Ube2b.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110565.
HOGENOMi HOG000233454.
HOVERGENi HBG063308.
InParanoidi P63149.
KOi K10574.
OMAi IVEQSWV.
OrthoDBi EOG7M0NTH.
PhylomeDBi P63149.
TreeFami TF101128.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

NextBioi 615721.
PROi P63149.

Gene expression databases

Genevestigatori P63149.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6."
    Wing S.S., Dumas F., Banville D.
    J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and regulation upon fasting and by insulin."
    Wing S.S., Banville D.
    Am. J. Physiol. 267:E39-E48(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
  3. "Isolation of a ubiquitin-conjugating enzyme gene induced following cerebral ischemia from the rat brain using modified subtractive hybridization and differential screening."
    Chen D., Graham S.H., Jing K., Simon R.P., Chen J.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  5. "The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by nerve growth factor and required for neurite outgrowth."
    Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L.
    Mol. Cell. Neurosci. 29:559-568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiUBE2B_RAT
AccessioniPrimary (citable) accession number: P63149
Secondary accession number(s): P23567, Q9D0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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