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P63149

- UBE2B_RAT

UniProt

P63149 - UBE2B_RAT

Protein

Ubiquitin-conjugating enzyme E2 B

Gene

Ube2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation By similarity. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.By similarity2 Publications

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. canonical Wnt signaling pathway Source: Ensembl
    2. cellular response to insulin stimulus Source: RGD
    3. chiasma assembly Source: Ensembl
    4. histone H2A ubiquitination Source: Ensembl
    5. histone lysine demethylation Source: Ensembl
    6. in utero embryonic development Source: Ensembl
    7. maintenance of chromatin silencing Source: Ensembl
    8. negative regulation of apoptotic process Source: Ensembl
    9. negative regulation of cAMP-mediated signaling Source: Ensembl
    10. negative regulation of histone phosphorylation Source: Ensembl
    11. positive regulation of reciprocal meiotic recombination Source: Ensembl
    12. postreplication repair Source: Ensembl
    13. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    14. protein autoubiquitination Source: Ensembl
    15. protein K11-linked ubiquitination Source: UniProtKB
    16. protein K48-linked ubiquitination Source: UniProtKB
    17. protein K63-linked ubiquitination Source: UniProtKB
    18. protein monoubiquitination Source: Ensembl
    19. protein stabilization Source: Ensembl
    20. response to drug Source: Ensembl
    21. response to hypoxia Source: RGD
    22. response to UV Source: Ensembl
    23. sperm axoneme assembly Source: Ensembl
    24. synaptonemal complex organization Source: Ensembl
    25. ubiquitin-dependent protein catabolic process Source: RGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
    Alternative name(s):
    RAD6 homolog B
    Short name:
    HR6B
    Ubiquitin carrier protein B
    Ubiquitin-conjugating enzyme E2(14k)
    Ubiquitin-protein ligase B
    Gene namesi
    Name:Ube2b
    Synonyms:Rad6b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi708345. Ube2b.

    Subcellular locationi

    Cell membrane 1 Publication. Nucleus 1 Publication
    Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. HULC complex Source: UniProtKB
    3. nuclear chromatin Source: Ensembl
    4. plasma membrane Source: UniProtKB-SubCell
    5. replication fork Source: Ensembl
    6. XY body Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152Ubiquitin-conjugating enzyme E2 BPRO_0000082450Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed at high levels in testes, moderate levels in muscle, heart, and brain, and low levels in liver and kidney. Upon fasting, increased levels were seen in muscle, heart, liver, and kidney.1 Publication

    Inductioni

    Up-regulated by NGF.1 Publication

    Gene expression databases

    GenevestigatoriP63149.

    Interactioni

    Subunit structurei

    Interacts with RAD18, UBR2 and WAC.By similarity

    Protein-protein interaction databases

    BioGridi249674. 1 interaction.
    STRINGi10116.ENSRNOP00000016742.

    Structurei

    3D structure databases

    ProteinModelPortaliP63149.
    SMRiP63149. Positions 3-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    InParanoidiP63149.
    KOiK10574.
    OMAiIVEQSWV.
    OrthoDBiEOG7M0NTH.
    PhylomeDBiP63149.
    TreeFamiTF101128.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED    50
    GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY 100
    DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN 150
    DS 152
    Length:152
    Mass (Da):17,312
    Last modified:September 27, 2004 - v1
    Checksum:iCFDEEEE7E06840BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62388 mRNA. Translation: AAA21087.1.
    U04308
    , U04303, U04304, U04305, U04306, U04307 Genomic DNA. Translation: AAB60669.1.
    AF144083 mRNA. Translation: AAD37966.1.
    BC070946 mRNA. Translation: AAH70946.1.
    PIRiI51913.
    RefSeqiNP_112400.1. NM_031138.2.
    UniGeneiRn.20766.

    Genome annotation databases

    EnsembliENSRNOT00000016742; ENSRNOP00000016742; ENSRNOG00000005064.
    GeneIDi81816.
    KEGGirno:81816.
    UCSCiRGD:708345. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62388 mRNA. Translation: AAA21087.1 .
    U04308
    , U04303 , U04304 , U04305 , U04306 , U04307 Genomic DNA. Translation: AAB60669.1 .
    AF144083 mRNA. Translation: AAD37966.1 .
    BC070946 mRNA. Translation: AAH70946.1 .
    PIRi I51913.
    RefSeqi NP_112400.1. NM_031138.2.
    UniGenei Rn.20766.

    3D structure databases

    ProteinModelPortali P63149.
    SMRi P63149. Positions 3-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249674. 1 interaction.
    STRINGi 10116.ENSRNOP00000016742.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016742 ; ENSRNOP00000016742 ; ENSRNOG00000005064 .
    GeneIDi 81816.
    KEGGi rno:81816.
    UCSCi RGD:708345. rat.

    Organism-specific databases

    CTDi 7320.
    RGDi 708345. Ube2b.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    InParanoidi P63149.
    KOi K10574.
    OMAi IVEQSWV.
    OrthoDBi EOG7M0NTH.
    PhylomeDBi P63149.
    TreeFami TF101128.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 615721.
    PROi P63149.

    Gene expression databases

    Genevestigatori P63149.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6."
      Wing S.S., Dumas F., Banville D.
      J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and regulation upon fasting and by insulin."
      Wing S.S., Banville D.
      Am. J. Physiol. 267:E39-E48(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    3. "Isolation of a ubiquitin-conjugating enzyme gene induced following cerebral ischemia from the rat brain using modified subtractive hybridization and differential screening."
      Chen D., Graham S.H., Jing K., Simon R.P., Chen J.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.
    5. "The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by nerve growth factor and required for neurite outgrowth."
      Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L.
      Mol. Cell. Neurosci. 29:559-568(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.

    Entry informationi

    Entry nameiUBE2B_RAT
    AccessioniPrimary (citable) accession number: P63149
    Secondary accession number(s): P23567, Q9D0J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3