Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P63149 (UBE2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 B

EC=6.3.2.19
Alternative name(s):
RAD6 homolog B
Short name=HR6B
Ubiquitin carrier protein B
Ubiquitin-conjugating enzyme E2(14k)
Ubiquitin-protein ligase B
Gene names
Name:Ube2b
Synonyms:Rad6b
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation By similarity. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. Ref.1 Ref.5

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with RAD18, UBR2 and WAC By similarity.

Subcellular location

Cell membrane. Nucleus. Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei. Ref.5

Tissue specificity

Expressed at high levels in testes, moderate levels in muscle, heart, and brain, and low levels in liver and kidney. Upon fasting, increased levels were seen in muscle, heart, liver, and kidney. Ref.2

Induction

Up-regulated by NGF. Ref.5

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Ubl conjugation pathway
   Cellular componentCell membrane
Membrane
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcanonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

cellular response to insulin stimulus

Inferred from expression pattern Ref.2. Source: RGD

chiasma assembly

Inferred from electronic annotation. Source: Ensembl

histone H2A ubiquitination

Inferred from electronic annotation. Source: Ensembl

histone lysine demethylation

Inferred from electronic annotation. Source: Ensembl

in utero embryonic development

Inferred from electronic annotation. Source: Ensembl

maintenance of chromatin silencing

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of cAMP-mediated signaling

Inferred from electronic annotation. Source: Ensembl

negative regulation of histone phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of reciprocal meiotic recombination

Inferred from electronic annotation. Source: Ensembl

postreplication repair

Inferred from electronic annotation. Source: Ensembl

proteasome-mediated ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: Ensembl

protein K11-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K48-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K63-linked ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein autoubiquitination

Inferred from electronic annotation. Source: Ensembl

protein monoubiquitination

Inferred from electronic annotation. Source: Ensembl

protein stabilization

Inferred from electronic annotation. Source: Ensembl

response to UV

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from expression pattern PubMed 16483544. Source: RGD

sperm axoneme assembly

Inferred from electronic annotation. Source: Ensembl

synaptonemal complex organization

Inferred from electronic annotation. Source: Ensembl

ubiquitin-dependent protein catabolic process

Inferred from expression pattern Ref.2. Source: RGD

   Cellular_componentHULC complex

Inferred from sequence or structural similarity. Source: UniProtKB

XY body

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

nuclear chromatin

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

replication fork

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 152152Ubiquitin-conjugating enzyme E2 B
PRO_0000082450

Sites

Active site881Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P63149 [UniParc].

Last modified September 27, 2004. Version 1.
Checksum: CFDEEEE7E06840BE

FASTA15217,312
        10         20         30         40         50         60 
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS 

        70         80         90        100        110        120 
EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 

       130        140        150 
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS 

« Hide

References

« Hide 'large scale' references
[1]"A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6."
Wing S.S., Dumas F., Banville D.
J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and regulation upon fasting and by insulin."
Wing S.S., Banville D.
Am. J. Physiol. 267:E39-E48(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
[3]"Isolation of a ubiquitin-conjugating enzyme gene induced following cerebral ischemia from the rat brain using modified subtractive hybridization and differential screening."
Chen D., Graham S.H., Jing K., Simon R.P., Chen J.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
[5]"The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by nerve growth factor and required for neurite outgrowth."
Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L.
Mol. Cell. Neurosci. 29:559-568(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62388 mRNA. Translation: AAA21087.1.
U04308 expand/collapse EMBL AC list , U04303, U04304, U04305, U04306, U04307 Genomic DNA. Translation: AAB60669.1.
AF144083 mRNA. Translation: AAD37966.1.
BC070946 mRNA. Translation: AAH70946.1.
PIRI51913.
RefSeqNP_112400.1. NM_031138.2.
UniGeneRn.20766.

3D structure databases

ProteinModelPortalP63149.
SMRP63149. Positions 3-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249674. 1 interaction.
STRING10116.ENSRNOP00000016742.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016742; ENSRNOP00000016742; ENSRNOG00000005064.
GeneID81816.
KEGGrno:81816.
UCSCRGD:708345. rat.

Organism-specific databases

CTD7320.
RGD708345. Ube2b.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00730000110565.
HOGENOMHOG000233454.
HOVERGENHBG063308.
InParanoidP63149.
KOK10574.
OMAIVEQSWV.
OrthoDBEOG7M0NTH.
PhylomeDBP63149.
TreeFamTF101128.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorP63149.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615721.
PROP63149.

Entry information

Entry nameUBE2B_RAT
AccessionPrimary (citable) accession number: P63149
Secondary accession number(s): P23567, Q9D0J6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways