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P63148

- UBE2B_RABIT

UniProt

P63148 - UBE2B_RABIT

Protein

Ubiquitin-conjugating enzyme E2 B

Gene

UBE2B

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. canonical Wnt signaling pathway Source: Ensembl
    2. chiasma assembly Source: Ensembl
    3. histone H2A ubiquitination Source: Ensembl
    4. histone lysine demethylation Source: Ensembl
    5. in utero embryonic development Source: Ensembl
    6. maintenance of chromatin silencing Source: Ensembl
    7. negative regulation of apoptotic process Source: Ensembl
    8. negative regulation of cAMP-mediated signaling Source: Ensembl
    9. negative regulation of histone phosphorylation Source: Ensembl
    10. positive regulation of reciprocal meiotic recombination Source: Ensembl
    11. postreplication repair Source: Ensembl
    12. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    13. protein autoubiquitination Source: Ensembl
    14. protein K11-linked ubiquitination Source: UniProtKB
    15. protein K48-linked ubiquitination Source: UniProtKB
    16. protein K63-linked ubiquitination Source: UniProtKB
    17. protein monoubiquitination Source: Ensembl
    18. protein stabilization Source: Ensembl
    19. response to drug Source: Ensembl
    20. response to UV Source: Ensembl
    21. sperm axoneme assembly Source: Ensembl
    22. synaptonemal complex organization Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
    Alternative name(s):
    RAD6 homolog B
    Short name:
    HR6B
    Ubiquitin carrier protein B
    Ubiquitin-conjugating enzyme E2(14k)
    Ubiquitin-protein ligase B
    Gene namesi
    Name:UBE2B
    Synonyms:RAD6B
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Chromosome 3

    Subcellular locationi

    Cell membrane By similarity. Nucleus By similarity
    Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. HULC complex Source: UniProtKB
    3. nuclear chromatin Source: Ensembl
    4. plasma membrane Source: UniProtKB-SubCell
    5. replication fork Source: Ensembl
    6. XY body Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152Ubiquitin-conjugating enzyme E2 BPRO_0000082449Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with RAD18, UBR2 and WAC.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP63148.
    SMRiP63148. Positions 3-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    OMAiIVEQSWV.
    OrthoDBiEOG7M0NTH.
    TreeFamiTF101128.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63148-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED    50
    GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY 100
    DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN 150
    DS 152
    Length:152
    Mass (Da):17,312
    Last modified:September 27, 2004 - v1
    Checksum:iCFDEEEE7E06840BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62387 mRNA. Translation: AAA31492.1.
    PIRiA42416.
    RefSeqiNP_001075765.1. NM_001082296.1.
    UniGeneiOcu.1605.

    Genome annotation databases

    EnsembliENSOCUT00000015769; ENSOCUP00000013548; ENSOCUG00000015774.
    GeneIDi100009132.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62387 mRNA. Translation: AAA31492.1 .
    PIRi A42416.
    RefSeqi NP_001075765.1. NM_001082296.1.
    UniGenei Ocu.1605.

    3D structure databases

    ProteinModelPortali P63148.
    SMRi P63148. Positions 3-152.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOCUT00000015769 ; ENSOCUP00000013548 ; ENSOCUG00000015774 .
    GeneIDi 100009132.

    Organism-specific databases

    CTDi 7320.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    OMAi IVEQSWV.
    OrthoDBi EOG7M0NTH.
    TreeFami TF101128.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6."
      Wing S.S., Dumas F., Banville D.
      J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiUBE2B_RABIT
    AccessioniPrimary (citable) accession number: P63148
    Secondary accession number(s): P23567, Q9D0J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3