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P63148

- UBE2B_RABIT

UniProt

P63148 - UBE2B_RABIT

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Protein
Ubiquitin-conjugating enzyme E2 B
Gene
UBE2B, RAD6B
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth By similarity.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. canonical Wnt signaling pathway Source: Ensembl
  2. chiasma assembly Source: Ensembl
  3. histone H2A ubiquitination Source: Ensembl
  4. histone lysine demethylation Source: Ensembl
  5. in utero embryonic development Source: Ensembl
  6. maintenance of chromatin silencing Source: Ensembl
  7. negative regulation of apoptotic process Source: Ensembl
  8. negative regulation of cAMP-mediated signaling Source: Ensembl
  9. negative regulation of histone phosphorylation Source: Ensembl
  10. positive regulation of reciprocal meiotic recombination Source: Ensembl
  11. postreplication repair Source: Ensembl
  12. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  13. protein K11-linked ubiquitination Source: UniProtKB
  14. protein K48-linked ubiquitination Source: UniProtKB
  15. protein K63-linked ubiquitination Source: UniProtKB
  16. protein autoubiquitination Source: Ensembl
  17. protein monoubiquitination Source: Ensembl
  18. protein stabilization Source: Ensembl
  19. response to UV Source: Ensembl
  20. response to drug Source: Ensembl
  21. sperm axoneme assembly Source: Ensembl
  22. synaptonemal complex organization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
Alternative name(s):
RAD6 homolog B
Short name:
HR6B
Ubiquitin carrier protein B
Ubiquitin-conjugating enzyme E2(14k)
Ubiquitin-protein ligase B
Gene namesi
Name:UBE2B
Synonyms:RAD6B
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Chromosome 3

Subcellular locationi

Cell membrane By similarity. Nucleus By similarity
Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei By similarity.

GO - Cellular componenti

  1. HULC complex Source: UniProtKB
  2. XY body Source: Ensembl
  3. cytoplasm Source: Ensembl
  4. nuclear chromatin Source: Ensembl
  5. plasma membrane Source: UniProtKB-SubCell
  6. replication fork Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 B
PRO_0000082449Add
BLAST

Interactioni

Subunit structurei

Interacts with RAD18, UBR2 and WAC By similarity.

Structurei

3D structure databases

ProteinModelPortaliP63148.
SMRiP63148. Positions 3-152.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
OMAiIVEQSWV.
OrthoDBiEOG7M0NTH.
TreeFamiTF101128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63148-1 [UniParc]FASTAAdd to Basket

« Hide

MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED    50
GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY 100
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN 150
DS 152
Length:152
Mass (Da):17,312
Last modified:September 27, 2004 - v1
Checksum:iCFDEEEE7E06840BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62387 mRNA. Translation: AAA31492.1.
PIRiA42416.
RefSeqiNP_001075765.1. NM_001082296.1.
UniGeneiOcu.1605.

Genome annotation databases

EnsembliENSOCUT00000015769; ENSOCUP00000013548; ENSOCUG00000015774.
GeneIDi100009132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62387 mRNA. Translation: AAA31492.1 .
PIRi A42416.
RefSeqi NP_001075765.1. NM_001082296.1.
UniGenei Ocu.1605.

3D structure databases

ProteinModelPortali P63148.
SMRi P63148. Positions 3-152.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSOCUT00000015769 ; ENSOCUP00000013548 ; ENSOCUG00000015774 .
GeneIDi 100009132.

Organism-specific databases

CTDi 7320.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110565.
HOGENOMi HOG000233454.
HOVERGENi HBG063308.
OMAi IVEQSWV.
OrthoDBi EOG7M0NTH.
TreeFami TF101128.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RAD6."
    Wing S.S., Dumas F., Banville D.
    J. Biol. Chem. 267:6495-6501(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiUBE2B_RABIT
AccessioniPrimary (citable) accession number: P63148
Secondary accession number(s): P23567, Q9D0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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