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Protein

Ubiquitin-conjugating enzyme E2 B

Gene

Ube2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • canonical Wnt signaling pathway Source: UniProtKB
  • cellular response to DNA damage stimulus Source: MGI
  • chiasma assembly Source: MGI
  • DNA repair Source: MGI
  • histone H2A ubiquitination Source: MGI
  • histone lysine demethylation Source: MGI
  • in utero embryonic development Source: MGI
  • maintenance of chromatin silencing Source: MGI
  • meiotic telomere clustering Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cAMP-mediated signaling Source: MGI
  • negative regulation of histone phosphorylation Source: MGI
  • positive regulation of reciprocal meiotic recombination Source: MGI
  • postreplication repair Source: MGI
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI
  • protein autoubiquitination Source: MGI
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein monoubiquitination Source: MGI
  • protein polyubiquitination Source: MGI
  • protein stabilization Source: MGI
  • protein ubiquitination Source: MGI
  • regulation of histone modification Source: MGI
  • response to drug Source: MGI
  • response to UV Source: MGI
  • spermatogenesis Source: MGI
  • sperm axoneme assembly Source: MGI
  • synaptonemal complex organization Source: MGI
  • ubiquitin-dependent protein catabolic process Source: MGI

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex
R-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 B (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme B
E214K
RAD6 homolog B
Short name:
HR6B
Short name:
mHR6B
Ubiquitin carrier protein B
Ubiquitin-protein ligase B
Gene namesi
Name:Ube2b
Synonyms:Rad6b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:102944 Ube2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824481 – 152Ubiquitin-conjugating enzyme E2 BAdd BLAST152

Proteomic databases

EPDiP63147
MaxQBiP63147
PaxDbiP63147
PRIDEiP63147

PTM databases

PhosphoSitePlusiP63147

Expressioni

Gene expression databases

BgeeiENSMUSG00000020390
ExpressionAtlasiP63147 baseline and differential
GenevisibleiP63147 MM

Interactioni

Subunit structurei

Interacts with RAD18, UBR2 and WAC.1 Publication

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: MGI

Protein-protein interaction databases

BioGridi204415, 3 interactors
STRINGi10090.ENSMUSP00000020657

Structurei

3D structure databases

ProteinModelPortaliP63147
SMRiP63147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00900000140930
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiP63147
KOiK10574
OMAiCHAIING
OrthoDBiEOG091G0UM8
PhylomeDBiP63147
TreeFamiTF101128

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P63147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED
60 70 80 90 100
GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN

DS
Length:152
Mass (Da):17,312
Last modified:September 27, 2004 - v1
Checksum:iCFDEEEE7E06840BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7R → E in BAB27570 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96859 mRNA Translation: CAA65602.1
U57690 mRNA Translation: AAC52884.1
AK010432 mRNA Translation: BAB26934.1
AK011363 mRNA Translation: BAB27570.1
AK147785 mRNA Translation: BAE28135.1
AK169229 mRNA Translation: BAE40998.1
CCDSiCCDS24664.1
RefSeqiNP_033484.3, NM_009458.4
XP_006533223.1, XM_006533160.2
XP_011247269.1, XM_011248967.1
UniGeneiMm.384918

Genome annotation databases

EnsembliENSMUST00000020657; ENSMUSP00000020657; ENSMUSG00000020390
ENSMUST00000109086; ENSMUSP00000104714; ENSMUSG00000020390
GeneIDi22210
KEGGimmu:22210
UCSCiuc007iut.2 mouse

Similar proteinsi

Entry informationi

Entry nameiUBE2B_MOUSE
AccessioniPrimary (citable) accession number: P63147
Secondary accession number(s): P23567, Q3UGS2, Q9D0J6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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