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P63147

- UBE2B_MOUSE

UniProt

P63147 - UBE2B_MOUSE

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Protein

Ubiquitin-conjugating enzyme E2 B

Gene

Ube2b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. canonical Wnt signaling pathway Source: UniProtKB
  2. cellular response to insulin stimulus Source: Ensembl
  3. chiasma assembly Source: MGI
  4. histone H2A ubiquitination Source: Ensembl
  5. histone lysine demethylation Source: MGI
  6. in utero embryonic development Source: MGI
  7. maintenance of chromatin silencing Source: MGI
  8. negative regulation of apoptotic process Source: MGI
  9. negative regulation of cAMP-mediated signaling Source: Ensembl
  10. negative regulation of histone phosphorylation Source: MGI
  11. positive regulation of reciprocal meiotic recombination Source: MGI
  12. postreplication repair Source: Ensembl
  13. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  14. protein autoubiquitination Source: Ensembl
  15. protein K11-linked ubiquitination Source: UniProtKB
  16. protein K48-linked ubiquitination Source: UniProtKB
  17. protein K63-linked ubiquitination Source: UniProtKB
  18. protein monoubiquitination Source: Ensembl
  19. protein stabilization Source: Ensembl
  20. regulation of histone modification Source: MGI
  21. response to drug Source: Ensembl
  22. response to hypoxia Source: Ensembl
  23. response to UV Source: Ensembl
  24. spermatogenesis Source: MGI
  25. sperm axoneme assembly Source: MGI
  26. synaptonemal complex organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
Alternative name(s):
E214K
RAD6 homolog B
Short name:
HR6B
Short name:
mHR6B
Ubiquitin carrier protein B
Ubiquitin-protein ligase B
Gene namesi
Name:Ube2b
Synonyms:Rad6b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:102944. Ube2b.

Subcellular locationi

Cell membrane By similarity. Nucleus By similarity
Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.By similarity

GO - Cellular componenti

  1. chromatin Source: MGI
  2. cytoplasm Source: Ensembl
  3. HULC complex Source: UniProtKB
  4. nuclear chromatin Source: MGI
  5. plasma membrane Source: UniProtKB-KW
  6. replication fork Source: Ensembl
  7. XY body Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 BPRO_0000082448Add
BLAST

Proteomic databases

MaxQBiP63147.
PaxDbiP63147.

Expressioni

Gene expression databases

BgeeiP63147.
ExpressionAtlasiP63147. baseline and differential.
GenevestigatoriP63147.

Interactioni

Subunit structurei

Interacts with RAD18, UBR2 and WAC.1 Publication

Protein-protein interaction databases

BioGridi204415. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP63147.
SMRiP63147. Positions 3-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP63147.
KOiK10574.
OMAiIVEQSWV.
OrthoDBiEOG7M0NTH.
PhylomeDBiP63147.
TreeFamiTF101128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63147-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED
60 70 80 90 100
GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN

DS
Length:152
Mass (Da):17,312
Last modified:September 27, 2004 - v1
Checksum:iCFDEEEE7E06840BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71R → E in BAB27570. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96859 mRNA. Translation: CAA65602.1.
U57690 mRNA. Translation: AAC52884.1.
AK010432 mRNA. Translation: BAB26934.1.
AK011363 mRNA. Translation: BAB27570.1.
AK147785 mRNA. Translation: BAE28135.1.
AK169229 mRNA. Translation: BAE40998.1.
CCDSiCCDS24664.1.
RefSeqiNP_033484.3. NM_009458.4.
XP_006533223.1. XM_006533160.1.
UniGeneiMm.384918.

Genome annotation databases

EnsembliENSMUST00000020657; ENSMUSP00000020657; ENSMUSG00000020390.
ENSMUST00000109086; ENSMUSP00000104714; ENSMUSG00000020390.
GeneIDi22210.
KEGGimmu:22210.
UCSCiuc007iut.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96859 mRNA. Translation: CAA65602.1 .
U57690 mRNA. Translation: AAC52884.1 .
AK010432 mRNA. Translation: BAB26934.1 .
AK011363 mRNA. Translation: BAB27570.1 .
AK147785 mRNA. Translation: BAE28135.1 .
AK169229 mRNA. Translation: BAE40998.1 .
CCDSi CCDS24664.1.
RefSeqi NP_033484.3. NM_009458.4.
XP_006533223.1. XM_006533160.1.
UniGenei Mm.384918.

3D structure databases

ProteinModelPortali P63147.
SMRi P63147. Positions 3-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204415. 1 interaction.

Proteomic databases

MaxQBi P63147.
PaxDbi P63147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000020657 ; ENSMUSP00000020657 ; ENSMUSG00000020390 .
ENSMUST00000109086 ; ENSMUSP00000104714 ; ENSMUSG00000020390 .
GeneIDi 22210.
KEGGi mmu:22210.
UCSCi uc007iut.2. mouse.

Organism-specific databases

CTDi 7320.
MGIi MGI:102944. Ube2b.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110565.
HOGENOMi HOG000233454.
HOVERGENi HBG063308.
InParanoidi P63147.
KOi K10574.
OMAi IVEQSWV.
OrthoDBi EOG7M0NTH.
PhylomeDBi P63147.
TreeFami TF101128.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

ChiTaRSi Ube2b. mouse.
NextBioi 28622.
PROi P63147.
SOURCEi Search...

Gene expression databases

Bgeei P63147.
ExpressionAtlasi P63147. baseline and differential.
Genevestigatori P63147.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification."
    Roest H.P., van Klaveren J., de Wit J., van Gurp C.G., Koken M.H.M., Vermey M., van Roijen J.H., Vreeburg J.T.M., Baarends W.M., Bootsma D., Grootegoed J.A., Hoeijmakers J.H.J.
    Cell 86:799-810(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Testis.
  2. Varshavsky A., Grigoryev S., Stewart A.E., Kwon Y.T., Arfin S.M., Bradshaw R.A., Jenkins N.A., Copeland N.G.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion and Embryo.
  4. "Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway."
    Kwon Y.T., Xia Z., An J.Y., Tasaki T., Davydov I.V., Seo J.W., Sheng J., Xie Y., Varshavsky A.
    Mol. Cell. Biol. 23:8255-8271(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBR2.

Entry informationi

Entry nameiUBE2B_MOUSE
AccessioniPrimary (citable) accession number: P63147
Secondary accession number(s): P23567, Q3UGS2, Q9D0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3