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P63147

- UBE2B_MOUSE

UniProt

P63147 - UBE2B_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 B

Gene

Ube2b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. canonical Wnt signaling pathway Source: UniProtKB
    2. cellular response to insulin stimulus Source: Ensembl
    3. chiasma assembly Source: MGI
    4. histone H2A ubiquitination Source: Ensembl
    5. histone lysine demethylation Source: MGI
    6. in utero embryonic development Source: MGI
    7. maintenance of chromatin silencing Source: MGI
    8. negative regulation of apoptotic process Source: MGI
    9. negative regulation of cAMP-mediated signaling Source: Ensembl
    10. negative regulation of histone phosphorylation Source: MGI
    11. positive regulation of reciprocal meiotic recombination Source: MGI
    12. postreplication repair Source: Ensembl
    13. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    14. protein autoubiquitination Source: Ensembl
    15. protein K11-linked ubiquitination Source: UniProtKB
    16. protein K48-linked ubiquitination Source: UniProtKB
    17. protein K63-linked ubiquitination Source: UniProtKB
    18. protein monoubiquitination Source: Ensembl
    19. protein stabilization Source: Ensembl
    20. regulation of histone modification Source: MGI
    21. response to drug Source: Ensembl
    22. response to hypoxia Source: Ensembl
    23. response to UV Source: Ensembl
    24. spermatogenesis Source: MGI
    25. sperm axoneme assembly Source: MGI
    26. synaptonemal complex organization Source: MGI

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 B (EC:6.3.2.19)
    Alternative name(s):
    E214K
    RAD6 homolog B
    Short name:
    HR6B
    Short name:
    mHR6B
    Ubiquitin carrier protein B
    Ubiquitin-protein ligase B
    Gene namesi
    Name:Ube2b
    Synonyms:Rad6b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:102944. Ube2b.

    Subcellular locationi

    Cell membrane By similarity. Nucleus By similarity
    Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.By similarity

    GO - Cellular componenti

    1. chromatin Source: MGI
    2. cytoplasm Source: Ensembl
    3. HULC complex Source: UniProtKB
    4. nuclear chromatin Source: MGI
    5. plasma membrane Source: UniProtKB-SubCell
    6. replication fork Source: Ensembl
    7. XY body Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152Ubiquitin-conjugating enzyme E2 BPRO_0000082448Add
    BLAST

    Proteomic databases

    PaxDbiP63147.

    Expressioni

    Gene expression databases

    ArrayExpressiP63147.
    BgeeiP63147.
    GenevestigatoriP63147.

    Interactioni

    Subunit structurei

    Interacts with RAD18, UBR2 and WAC.1 Publication

    Protein-protein interaction databases

    BioGridi204415. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP63147.
    SMRiP63147. Positions 3-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    InParanoidiP63147.
    KOiK10574.
    OMAiIVEQSWV.
    OrthoDBiEOG7M0NTH.
    PhylomeDBiP63147.
    TreeFamiTF101128.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63147-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED    50
    GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY 100
    DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN 150
    DS 152
    Length:152
    Mass (Da):17,312
    Last modified:September 27, 2004 - v1
    Checksum:iCFDEEEE7E06840BE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71R → E in BAB27570. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96859 mRNA. Translation: CAA65602.1.
    U57690 mRNA. Translation: AAC52884.1.
    AK010432 mRNA. Translation: BAB26934.1.
    AK011363 mRNA. Translation: BAB27570.1.
    AK147785 mRNA. Translation: BAE28135.1.
    AK169229 mRNA. Translation: BAE40998.1.
    CCDSiCCDS24664.1.
    RefSeqiNP_033484.3. NM_009458.4.
    XP_006533223.1. XM_006533160.1.
    UniGeneiMm.384918.

    Genome annotation databases

    EnsembliENSMUST00000020657; ENSMUSP00000020657; ENSMUSG00000020390.
    ENSMUST00000109086; ENSMUSP00000104714; ENSMUSG00000020390.
    GeneIDi22210.
    KEGGimmu:22210.
    UCSCiuc007iut.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96859 mRNA. Translation: CAA65602.1 .
    U57690 mRNA. Translation: AAC52884.1 .
    AK010432 mRNA. Translation: BAB26934.1 .
    AK011363 mRNA. Translation: BAB27570.1 .
    AK147785 mRNA. Translation: BAE28135.1 .
    AK169229 mRNA. Translation: BAE40998.1 .
    CCDSi CCDS24664.1.
    RefSeqi NP_033484.3. NM_009458.4.
    XP_006533223.1. XM_006533160.1.
    UniGenei Mm.384918.

    3D structure databases

    ProteinModelPortali P63147.
    SMRi P63147. Positions 3-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204415. 1 interaction.

    Proteomic databases

    PaxDbi P63147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020657 ; ENSMUSP00000020657 ; ENSMUSG00000020390 .
    ENSMUST00000109086 ; ENSMUSP00000104714 ; ENSMUSG00000020390 .
    GeneIDi 22210.
    KEGGi mmu:22210.
    UCSCi uc007iut.2. mouse.

    Organism-specific databases

    CTDi 7320.
    MGIi MGI:102944. Ube2b.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    InParanoidi P63147.
    KOi K10574.
    OMAi IVEQSWV.
    OrthoDBi EOG7M0NTH.
    PhylomeDBi P63147.
    TreeFami TF101128.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi UBE2B. mouse.
    NextBioi 28622.
    PROi P63147.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63147.
    Bgeei P63147.
    Genevestigatori P63147.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification."
      Roest H.P., van Klaveren J., de Wit J., van Gurp C.G., Koken M.H.M., Vermey M., van Roijen J.H., Vreeburg J.T.M., Baarends W.M., Bootsma D., Grootegoed J.A., Hoeijmakers J.H.J.
      Cell 86:799-810(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Testis.
    2. Varshavsky A., Grigoryev S., Stewart A.E., Kwon Y.T., Arfin S.M., Bradshaw R.A., Jenkins N.A., Copeland N.G.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Amnion and Embryo.
    4. "Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway."
      Kwon Y.T., Xia Z., An J.Y., Tasaki T., Davydov I.V., Seo J.W., Sheng J., Xie Y., Varshavsky A.
      Mol. Cell. Biol. 23:8255-8271(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UBR2.

    Entry informationi

    Entry nameiUBE2B_MOUSE
    AccessioniPrimary (citable) accession number: P63147
    Secondary accession number(s): P23567, Q3UGS2, Q9D0J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3