P63147 (UBE2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 B
EC=6.3.2.19

Alternative name(s):
E214K
RAD6 homolog B
Short name=HR6B
Short name=mHR6B
Ubiquitin carrier protein B
Ubiquitin-protein ligase B

Gene names

Name:	Ube2b
Synonyms:	Rad6b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RAD18, UBR2 and WAC. Ref.4
Subcellular location	Cell membrane By similarity. Nucleus By similarity. Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Cell membrane Membrane Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	canonical Wnt receptor signaling pathway Inferred from mutant phenotype PubMed 18339854. Source: UniProtKB cellular response to insulin stimulus Inferred from electronic annotation. Source: Compara chiasma assembly Inferred from mutant phenotype PubMed 12556476. Source: MGI histone H2A ubiquitination Inferred from electronic annotation. Source: Compara histone lysine demethylation Inferred from mutant phenotype PubMed 17488778. Source: MGI in utero embryonic development Inferred from genetic interaction PubMed 15169909. Source: MGI maintenance of chromatin silencing Inferred from mutant phenotype PubMed 17488778. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 12556476. Source: MGI negative regulation of cAMP-mediated signaling Inferred from electronic annotation. Source: Compara negative regulation of histone phosphorylation Inferred from mutant phenotype PubMed 17488778. Source: MGI positive regulation of reciprocal meiotic recombination Inferred from mutant phenotype PubMed 12556476. Source: MGI postreplication repair Inferred from electronic annotation. Source: Compara proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein autoubiquitination Inferred from electronic annotation. Source: Compara protein monoubiquitination Inferred from electronic annotation. Source: Compara protein stabilization Inferred from electronic annotation. Source: Compara response to UV Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara sperm axoneme assembly Inferred from mutant phenotype PubMed 12672659. Source: MGI synaptonemal complex organization Inferred from mutant phenotype PubMed 12556476PubMed 17488778. Source: MGI
Cellular_component	HULC complex Inferred from sequence or structural similarity. Source: UniProtKB XY body Inferred from direct assay PubMed 15383616. Source: MGI cytoplasm Inferred from electronic annotation. Source: Compara nuclear chromatin Inferred from direct assay PubMed 15383616. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell replication fork Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Ubiquitin-conjugating enzyme E2 B		PRO_0000082448
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	7	1	R → E in BAB27570. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P63147 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: CFDEEEE7E06840BE	FASTA	152	17,312
	10 20 30 40 50 60 MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS 70 80 90 100 110 120 EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 130 140 150 PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification." Roest H.P., van Klaveren J., de Wit J., van Gurp C.G., Koken M.H.M., Vermey M., van Roijen J.H., Vreeburg J.T.M., Baarends W.M., Bootsma D., Grootegoed J.A., Hoeijmakers J.H.J. Cell 86:799-810(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Testis.
[2]	Varshavsky A., Grigoryev S., Stewart A.E., Kwon Y.T., Arfin S.M., Bradshaw R.A., Jenkins N.A., Copeland N.G. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Amnion and Embryo.
[4]	"Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway." Kwon Y.T., Xia Z., An J.Y., Tasaki T., Davydov I.V., Seo J.W., Sheng J., Xie Y., Varshavsky A. Mol. Cell. Biol. 23:8255-8271(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBR2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96859 mRNA. Translation: CAA65602.1. U57690 mRNA. Translation: AAC52884.1. AK010432 mRNA. Translation: BAB26934.1. AK011363 mRNA. Translation: BAB27570.1. AK147785 mRNA. Translation: BAE28135.1. AK169229 mRNA. Translation: BAE40998.1.
IPI	IPI00133595.
RefSeq	NP_033484.3. NM_009458.4.
UniGene	Mm.384918. Mm.471916.
3D structure databases
ProteinModelPortal	P63147.
ModBase	Search...
Proteomic databases
PaxDb	P63147.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020657; ENSMUSP00000020657; ENSMUSG00000020390. ENSMUST00000109086; ENSMUSP00000104714; ENSMUSG00000020390.
GeneID	22210.
KEGG	mmu:22210.
Organism-specific databases
CTD	7320.
MGI	MGI:102944. Ube2b.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00680000099547.
HOGENOM	HOG000233454.
HOVERGEN	HBG063308.
InParanoid	P63147.
KO	K10574.
OMA	VIFGPVG.
OrthoDB	EOG4RJG2S.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P63147.
Bgee	P63147.
Genevestigator	P63147.
GermOnline	ENSMUSG00000020390. Mus musculus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	UBE2B. mouse.
NextBio	28622.
SOURCE	Search...

Entry information

Entry name

UBE2B_MOUSE

Accession

Primary (citable) accession number: P63147
Secondary accession number(s): P23567, Q3UGS2, Q9D0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program