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Reviewed, UniProtKB/Swiss-Prot P63146 (UBE2B_HUMAN)

Last modified November 4, 2008. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ubiquitin-conjugating enzyme E2 B
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase B
    Ubiquitin carrier protein B
    HR6B
    hHR6B
    E2-17 kDa
Gene names
Name: UBE2B
Synonyms: RAD6B
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with RAD18 and UBR2.

Subcellular location

Cell membraneBy similarity. NucleusBy similarity. Note= In peripheral neurons, expressed both at the plasma membrane and in nuclei By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords

   Biological processDNA damage
DNA repair
Ubl conjugation pathway
   Cellular componentCell membrane
Membrane
Nucleus
   Molecular functionLigase
   Technical term3D-structure
Direct protein sequencing

Gene Ontology (GO)

   Biological processpostreplication repair

Traceable author statement. Source: ProtInc

ubiquitin-dependent protein catabolic process

Traceable author statement. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 152152Ubiquitin-conjugating enzyme E2 B
PRO_0000082447

Sites

Active site881Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict22 – 232VG → C Ref.2
Sequence conflict411F → I Ref.2
Sequence conflict541K → R Ref.2

Secondary structure

......................... 152
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P63146-1 [UniParc].

Last modified September 27, 2004. Version 1.
Checksum: CFDEEEE7E06840BE

FASTA15217,312
        10         20         30         40         50         60 
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS 

        70         80         90        100        110        120 
EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 

       130        140        150 
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS 

« Hide

References

« Hide 'large scale' references
[1]"The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6."
Schneider R., Eckerskorn C., Lottspeich F., Schweiger M.
EMBO J. 9:1431-1435(1990) [PubMed: 2158443] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Placenta.
[2]"Mammalian mRNAs encoding protein closely related to ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6."
Woffendin C., Chen Z.Y., Staskus K., Retzel E.F., Plagemann P.G.
Biochim. Biophys. Acta 1090:81-85(1991) [PubMed: 1883845] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6."
Koken M.H.M., Reynolds P., Jaspers-Dekker I., Prakash L., Prakash S., Bootsma D., Hoeijmakers J.H.J.
Proc. Natl. Acad. Sci. U.S.A. 88:8865-8869(1991) [PubMed: 1717990] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)."
Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N., Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B., Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J., Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A.
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow and Brain.
[8]"The human RAD18 gene product interacts with HHR6A and HHR6B."
Xin H., Lin W., Sumanasekera W., Zhang Y., Wu X., Wang Z.
Nucleic Acids Res. 28:2847-2854(2000) [PubMed: 10908344] [Abstract]
Cited for: INTERACTION WITH RAD18.
[9]"Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination."
Motegi A., Sood R., Moinova H., Markowitz S.D., Liu P.P., Myung K.
J. Cell Biol. 175:703-708(2006) [PubMed: 17130289] [Abstract]
Cited for: FUNCTION.
[10]"Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen."
Unk I., Hajdu I., Fatyol K., Szakal B., Blastyak A., Bermudez V., Hurwitz J., Prakash L., Prakash S., Haracska L.
Proc. Natl. Acad. Sci. U.S.A. 103:18107-18112(2006) [PubMed: 17108083] [Abstract]
Cited for: FUNCTION.
[11]"The NMR structure of the class I human ubiquitin-conjugating enzyme 2b."
Miura T., Klaus W., Ross A., Guntert P., Senn H.
J. Biomol. NMR 22:89-92(2002) [PubMed: 11885984] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

M74525 mRNA. Translation: AAA35982.1.
X53251 mRNA. Translation: CAA37339.1.
BT007071 mRNA. Translation: AAP35734.1.
CR407634 mRNA. Translation: CAG28562.1.
DQ090910 Genomic DNA. Translation: AAY68224.1.
BC005979 mRNA. Translation: AAH05979.1.
BC008404 mRNA. Translation: AAH08404.1.
BC008470 mRNA. Translation: AAH08470.1.
PIRB41222.
RefSeqNP_003328.1.
UniGeneHs.615284
Hs.695927

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1JASNMR-A1-152[»]
1NXAmodel-A1-152[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP63146.

Polymorphism databases

NIEHS-SNPsSearch...

Genome annotation databases

EnsemblENSG00000119048. Homo sapiens. [Contig view]
GeneID7320.
KEGGhsa:7320.

Organism-specific databases

H-InvDBHIX0005175.
HGNCHGNC:12473. UBE2B.
MIM179095. gene.
PharmGKBPA37123.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP63146.
HOVERGENP63146.

Gene expression databases

ArrayExpressP63146.
CleanExHS_UBE2B.
GermOnlineENSG00000119048. Homo sapiens.

Family and domain databases

InterProIPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]
Gene3DG3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHERPTHR11621. UBQ-conjugat_E2. 1 hit.
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
ProDomPD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00212. UBCc. 1 hit.
[Graphical view]
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio28622.
SOURCESearch...

Entry information

Entry nameUBE2B_HUMAN
AccessionPrimary (citable) accession number: P63146
Secondary accession number(s): P23567, Q4PJ15, Q9D0J6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 4, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents