Reviewed, UniProtKB/Swiss-Prot P63146 (UBE2B_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 B
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase B
    Ubiquitin carrier protein B
    HR6B
    hHR6B
    E2-17 kDa

Gene names

Name:	UBE2B
Synonyms:	RAD6B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RAD18 and UBR2.
Subcellular location	Cell membraneBy similarity. NucleusBy similarity. Note= In peripheral neurons, expressed both at the plasma membrane and in nuclei By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Cell membrane Membrane Nucleus
Molecular function	Ligase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	postreplication repair Traceable author statement. Source: ProtInc ubiquitin-dependent protein catabolic process Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 152

152

Ubiquitin-conjugating enzyme E2 B

PRO_0000082447

Sites

Active site

88

1

Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict

22 – 23

2

VG → C Ref.2

Sequence conflict

41

1

F → I Ref.2

Sequence conflict

54

1

K → R Ref.2

Secondary structure

1

.

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152

Helix Strand Turn

Details...

Helix

4 – 18

15

Beta strand

22 – 28

7

Beta strand

33 – 41

9

Beta strand

52 – 58

7

Turn

63 – 65

3

Beta strand

69 – 74

6

Beta strand

85 – 87

3

Helix

90 – 92

3

Turn

93 – 95

3

Helix

102 – 114

13

Helix

124 – 131

8

Helix

134 – 146

13

Helix

147 – 150

4

Sequences

	Sequence		Length	Mass (Da)
	P63146-1 [UniParc]. Last modified September 27, 2004. Version 1. Checksum: CFDEEEE7E06840BE	FASTA	152	17,312
	10 20 30 40 50 60 MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS 70 80 90 100 110 120 EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS 130 140 150 PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6." Schneider R., Eckerskorn C., Lottspeich F., Schweiger M. EMBO J. 9:1431-1435(1990) [PubMed: 2158443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Placenta.
[2]	"Mammalian mRNAs encoding protein closely related to ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6." Woffendin C., Chen Z.Y., Staskus K., Retzel E.F., Plagemann P.G. Biochim. Biophys. Acta 1090:81-85(1991) [PubMed: 1883845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6." Koken M.H.M., Reynolds P., Jaspers-Dekker I., Prakash L., Prakash S., Bootsma D., Hoeijmakers J.H.J. Proc. Natl. Acad. Sci. U.S.A. 88:8865-8869(1991) [PubMed: 1717990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N., Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B., Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J., Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Brain.
[8]	"The human RAD18 gene product interacts with HHR6A and HHR6B." Xin H., Lin W., Sumanasekera W., Zhang Y., Wu X., Wang Z. Nucleic Acids Res. 28:2847-2854(2000) [PubMed: 10908344] [Abstract] Cited for: INTERACTION WITH RAD18.
[9]	"Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination." Motegi A., Sood R., Moinova H., Markowitz S.D., Liu P.P., Myung K. J. Cell Biol. 175:703-708(2006) [PubMed: 17130289] [Abstract] Cited for: FUNCTION.
[10]	"Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen." Unk I., Hajdu I., Fatyol K., Szakal B., Blastyak A., Bermudez V., Hurwitz J., Prakash L., Prakash S., Haracska L. Proc. Natl. Acad. Sci. U.S.A. 103:18107-18112(2006) [PubMed: 17108083] [Abstract] Cited for: FUNCTION.
[11]	"The NMR structure of the class I human ubiquitin-conjugating enzyme 2b." Miura T., Klaus W., Ross A., Guntert P., Senn H. J. Biomol. NMR 22:89-92(2002) [PubMed: 11885984] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M74525 mRNA. Translation: AAA35982.1.
X53251 mRNA. Translation: CAA37339.1.
BT007071 mRNA. Translation: AAP35734.1.
CR407634 mRNA. Translation: CAG28562.1.
DQ090910 Genomic DNA. Translation: AAY68224.1.
BC005979 mRNA. Translation: AAH05979.1.
BC008404 mRNA. Translation: AAH08404.1.
BC008470 mRNA. Translation: AAH08470.1.

PIR

B41222.

RefSeq

NP_003328.1.

UniGene

Hs.615284
Hs.695927

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JAS	NMR	-	A	1-152	[»]
1NXA	model	-	A	1-152	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P63146.

Polymorphism databases

NIEHS-SNPs

Search...

Genome annotation databases

Ensembl

ENSG00000119048. Homo sapiens. [Contig view]

GeneID

7320.

KEGG

hsa:7320.

Organism-specific databases

H-InvDB

HIX0005175.

HGNC

HGNC:12473. UBE2B.

MIM

179095. gene.

PharmGKB

PA37123.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P63146.

HOVERGEN

P63146.

Gene expression databases

ArrayExpress

P63146.

CleanEx

HS_UBE2B.

GermOnline

ENSG00000119048. Homo sapiens.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

PANTHER

PTHR11621. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

BLOCKS

Search...

ProtoNet

Search...

Other Resources

NextBio

28622.

SOURCE

Search...

Entry information

Entry name

UBE2B_HUMAN

Accession

Primary (citable) accession number: P63146
Secondary accession number(s): P23567, Q4PJ15, Q9D0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	November 4, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)