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Protein

Voltage-gated potassium channel subunit beta-1

Gene

Kcnab1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits. Modulates action potentials via its effect on the pore-forming alpha subunits (Probable). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members (PubMed:8183366, PubMed:15618540, PubMed:18222921). Promotes the closure of KCNA1, KCNA2 and KCNA5 channels (PubMed:10064591, PubMed:10650996, PubMed:16504945). Accelerates KCNA4 channel closure (PubMed:8183366). Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4 (PubMed:16504945). Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (PubMed:15618540). Enhances KCNB1 and KCNB2 channel activity (By similarity). Binds NADPH; this is required for efficient down-regulation of potassium channel activity. Has NADPH-dependent aldoketoreductase activity (PubMed:18222921). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (PubMed:18222921, PubMed:21436029).By similarityCurated7 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971NADPBy similarity
Binding sitei119 – 1191NADPBy similarity
Active sitei124 – 1241Proton donor/acceptorBy similarity
Binding sitei124 – 1241NADPBy similarity
Binding sitei248 – 2481NADPBy similarity
Binding sitei288 – 2881NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 912NADPBy similarity
Nucleotide bindingi222 – 2232NADPBy similarity
Nucleotide bindingi277 – 2826NADPBy similarity
Nucleotide bindingi357 – 3637NADPBy similarity

GO - Molecular functioni

  • aldo-keto reductase (NADP) activity Source: UniProtKB
  • ion channel binding Source: RGD
  • NADPH binding Source: UniProtKB
  • potassium channel regulator activity Source: UniProtKB
  • voltage-gated potassium channel activity Source: InterPro

GO - Biological processi

  • brain development Source: RGD
  • diaphragm development Source: RGD
  • heart development Source: RGD
  • learning or memory Source: Ensembl
  • myoblast differentiation Source: RGD
  • negative regulation of delayed rectifier potassium channel activity Source: RGD
  • oxidation-reduction process Source: UniProtKB
  • regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • skeletal muscle tissue development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

NADP, Potassium

Enzyme and pathway databases

ReactomeiR-RNO-1296072. Voltage gated Potassium channels.

Protein family/group databases

TCDBi8.A.5.1.3. the voltage-gated k(+) channel -subunit (kv) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated potassium channel subunit beta-1 (EC:1.1.1.-1 Publication)
Alternative name(s):
K(+) channel subunit beta-1
Kv-beta-1
Gene namesi
Name:Kcnab1
Synonyms:Kvb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi61827. Kcnab1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cytosol Source: UniProtKB
  • dendrite cytoplasm Source: RGD
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • juxtaparanode region of axon Source: RGD
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • perikaryon Source: RGD
  • plasma membrane Source: RGD
  • potassium channel complex Source: UniProtKB
  • proximal dendrite Source: RGD
  • voltage-gated potassium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi152 – 1521K → M: Loss of enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Voltage-gated potassium channel subunit beta-1PRO_0000148743Add
BLAST

Proteomic databases

PaxDbiP63144.
PRIDEiP63144.

Expressioni

Tissue specificityi

Detected in brain (PubMed:9334400). Detected in hippocampus, in the middle third of the molecular layer of the dentate gyrus and in the mossy fiber zone of the CA3 region (PubMed:9334400). Detected in globus pallidus and pars reticulata of the substantia nigra (at protein level) (PubMed:9334400). Specifically expressed in the nervous system (PubMed:8183366). Detected in mesenteric arteries (PubMed:15618540).3 Publications

Gene expression databases

GenevisibleiP63144. RN.

Interactioni

Subunit structurei

Homotetramer (PubMed:18222921). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (Probable). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (PubMed:9334400, PubMed:16504945). Interacts with KCNA1 (PubMed:10064591). Interacts with the dimer formed by GNB1 and GNG2; this enhances KCNA1 binding (PubMed:10064591). Interacts with KCNA4 (By similarity). Interacts with KCNB2 and KCNA5 (By similarity). Interacts with SQSTM1 (PubMed:10477520). Part of a complex containing KCNA1, KCNA4 and LGI1; interaction with LGI1 inhibits down-regulation of KCNA1 channel activity (PubMed:16504945).By similarityCurated5 Publications

GO - Molecular functioni

  • ion channel binding Source: RGD

Protein-protein interaction databases

BioGridi248349. 2 interactions.
STRINGi10116.ENSRNOP00000051084.

Structurei

3D structure databases

ProteinModelPortaliP63144.
SMRiP63144. Positions 73-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal domain of the beta subunit mediates closure of delayed rectifier potassium channels by physically obstructing the pore.3 Publications

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
GeneTreeiENSGT00550000074567.
HOGENOMiHOG000250283.
HOVERGENiHBG052216.
InParanoidiP63144.
KOiK04882.
OMAiMGLVVWS.
OrthoDBiEOG71G9TR.
PhylomeDBiP63144.
TreeFamiTF324563.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005400. K_chnl_volt-dep_bsu_KCNAB1.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01578. KCNAB1CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.

Sequencei

Sequence statusi: Complete.

P63144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVSIACTEH NLKSRNGEDR LLSKQSSTAP NVVNAARAKF RTVAIIARSL
60 70 80 90 100
GTFTPQHHIS LKESTAKQTG MKYRNLGKSG LRVSCLGLGT WVTFGGQISD
110 120 130 140 150
EVAERLMTIA YESGVNLFDT AEVYAAGKAE VILGSIIKKK GWRRSSLVIT
160 170 180 190 200
TKLYWGGKAE TERGLSRKHI IEGLKGSLQR LQLEYVDVVF ANRPDSNTPM
210 220 230 240 250
EEIVRAMTHV INQGMAMYWG TSRWSAMEIM EAYSVARQFN MIPPVCEQAE
260 270 280 290 300
YHLFQREKVE VQLPELYHKI GVGAMTWSPL ACGIISGKYG NGVPESSRAS
310 320 330 340 350
LKCYQWLKER IVSEEGRKQQ NKLKDLSPIA ERLGCTLPQL AVAWCLRNEG
360 370 380 390 400
VSSVLLGSST PEQLIENLGA IQVLPKMTSH VVNEIDNILR NKPYSKKDYR

S
Length:401
Mass (Da):44,710
Last modified:September 13, 2004 - v1
Checksum:iF9F17404DF35FDA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70662 mRNA. Translation: CAA50000.1.
BC089219 mRNA. Translation: AAH89219.1.
RefSeqiNP_058999.1. NM_017303.2.
UniGeneiRn.32090.

Genome annotation databases

EnsembliENSRNOT00000077973; ENSRNOP00000072289; ENSRNOG00000056697.
GeneIDi29737.
KEGGirno:29737.
UCSCiRGD:61827. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70662 mRNA. Translation: CAA50000.1.
BC089219 mRNA. Translation: AAH89219.1.
RefSeqiNP_058999.1. NM_017303.2.
UniGeneiRn.32090.

3D structure databases

ProteinModelPortaliP63144.
SMRiP63144. Positions 73-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248349. 2 interactions.
STRINGi10116.ENSRNOP00000051084.

Protein family/group databases

TCDBi8.A.5.1.3. the voltage-gated k(+) channel -subunit (kv) family.

Proteomic databases

PaxDbiP63144.
PRIDEiP63144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000077973; ENSRNOP00000072289; ENSRNOG00000056697.
GeneIDi29737.
KEGGirno:29737.
UCSCiRGD:61827. rat.

Organism-specific databases

CTDi7881.
RGDi61827. Kcnab1.

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
GeneTreeiENSGT00550000074567.
HOGENOMiHOG000250283.
HOVERGENiHBG052216.
InParanoidiP63144.
KOiK04882.
OMAiMGLVVWS.
OrthoDBiEOG71G9TR.
PhylomeDBiP63144.
TreeFamiTF324563.

Enzyme and pathway databases

ReactomeiR-RNO-1296072. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi289811.
PROiP63144.

Gene expression databases

GenevisibleiP63144. RN.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005400. K_chnl_volt-dep_bsu_KCNAB1.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01578. KCNAB1CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit."
    Rettig J., Heinemann S.H., Wunder F., Lorra C., Parcej D.N., Dolly J.O., Pongs O.
    Nature 369:289-294(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DOMAIN.
    Tissue: Brain cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes."
    Rhodes K.J., Strassle B.W., Monaghan M.M., Bekele-Arcuri Z., Matos M.F., Trimmer J.S.
    J. Neurosci. 17:8246-8258(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNA1; KCNA2; KCNA4; KCNA6 AND KCNAB2, SUBUNIT, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  4. "Fast inactivation of a brain K+ channel composed of Kv1.1 and Kvbeta1.1 subunits modulated by G protein beta gamma subunits."
    Jing J., Chikvashvili D., Singer-Lahat D., Thornhill W.B., Reuveny E., Lotan I.
    EMBO J. 18:1245-1256(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH KCNA1; GNB1 AND GNG2.
  5. "Modal behavior of the Kv1.1 channel conferred by the Kvbeta1.1 subunit and its regulation by dephosphorylation of Kv1.1."
    Singer-Lahat D., Dascal N., Lotan I.
    Pflugers Arch. 439:18-26(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  6. "Differential stimulation of PKC phosphorylation of potassium channels by ZIP1 and ZIP2."
    Gong J., Xu J., Bezanilla M., van Huizen R., Derin R., Li M.
    Science 285:1565-1569(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SQSTM1.
  7. "Heteromultimeric Kv1 channels contribute to myogenic control of arterial diameter."
    Plane F., Johnson R., Kerr P., Wiehler W., Thorneloe K., Ishii K., Chen T., Cole W.
    Circ. Res. 96:216-224(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
  8. "The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1."
    Schulte U., Thumfart J.-O., Kloecker N., Sailer C.A., Bildl W., Biniossek M., Dehn D., Deller T., Eble S., Abbass K., Wangler T., Knaus H.-G., Fakler B.
    Neuron 49:697-706(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LGI1; KCNA1 AND KCNA4, SUBCELLULAR LOCATION, SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Functional coupling between the Kv1.1 channel and aldoketoreductase Kvbeta1."
    Pan Y., Weng J., Cao Y., Bhosle R.C., Zhou M.
    J. Biol. Chem. 283:8634-8642(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, DOMAIN, MUTAGENESIS OF LYS-152.
  10. "The molecular basis for the actions of Kvbeta1.2 on the opening and closing of the Kv1.2 delayed rectifier channel."
    Peters C.J., Vaid M., Horne A.J., Fedida D., Accili E.A.
    Channels 3:314-322(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNA2.
  11. "Oxidation of NADPH on Kvbeta1 inhibits ball-and-chain type inactivation by restraining the chain."
    Pan Y., Weng J., Levin E.J., Zhou M.
    Proc. Natl. Acad. Sci. U.S.A. 108:5885-5890(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.

Entry informationi

Entry nameiKCAB1_RAT
AccessioniPrimary (citable) accession number: P63144
Secondary accession number(s): P97380
, Q5FWS9, Q61763, Q63277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: March 16, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.