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P63142

- KCNA2_RAT

UniProt

P63142 - KCNA2_RAT

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Protein
Potassium voltage-gated channel subfamily A member 2
Gene
Kcna2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.1 Publication

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: RefGenome
  2. outward rectifier potassium channel activity Source: RGD
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. optic nerve structural organization Source: Ensembl
  2. protein homooligomerization Source: InterPro
  3. protein oligomerization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 2
Alternative name(s):
RAK
RBK2
RCK5
Voltage-gated potassium channel subunit Kv1.2
Gene namesi
Name:Kcna2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi2950. Kcna2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei164 – 18219Helical; Name=Segment S1
Add
BLAST
Transmembranei222 – 24322Helical; Name=Segment S2
Add
BLAST
Transmembranei255 – 27521Helical; Name=Segment S3
Add
BLAST
Transmembranei293 – 31119Helical; Voltage-sensor; Name=Segment S4
Add
BLAST
Transmembranei328 – 34720Helical; Name=Segment S5
Add
BLAST
Transmembranei389 – 41123Helical; Name=Segment S6
Add
BLAST

GO - Cellular componenti

  1. juxtaparanode region of axon Source: BHF-UCL
  2. voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341R → L: No effect on channel opening. 1 Publication
Mutagenesisi38 – 381N → A: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi40 – 401S → A: No effect on channel opening. 1 Publication
Mutagenesisi41 – 411G → A: Loss of channel activity. 1 Publication
Mutagenesisi42 – 421L → A: No effect on channel opening. 1 Publication
Mutagenesisi43 – 431R → A: No effect on channel opening. 1 Publication
Mutagenesisi44 – 441F → A: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi45 – 451E → A: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi46 – 461T → D: Loss of tetramerization and channel activity. 1 Publication
Mutagenesisi46 – 461T → V or A: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi47 – 471Q → A: No effect on channel opening. 1 Publication
Mutagenesisi50 – 501T → A: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi70 – 701D → A: No effect on channel opening. 1 Publication
Mutagenesisi73 – 731R → A: No effect on channel opening. 1 Publication
Mutagenesisi75 – 751E → A: No effect on channel opening. 1 Publication
Mutagenesisi77 – 771F → W: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi79 – 791D → N: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi81 – 811N → A: No effect on channel opening. 1 Publication
Mutagenesisi82 – 821R → A: Loss of channel activity. 1 Publication
Mutagenesisi86 – 861D → A: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi89 – 891L → A: No effect on channel opening. 1 Publication
Mutagenesisi90 – 901Y → A: No effect on channel opening. 1 Publication
Mutagenesisi93 – 931Q → A: Loss of channel activity. 1 Publication
Mutagenesisi97 – 971R → A: No effect on channel opening. 1 Publication
Mutagenesisi99 – 991R → A: No effect on channel opening. 1 Publication
Mutagenesisi102 – 1021V → T: Interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi103 – 1031N → A: No effect on channel opening. 1 Publication
Mutagenesisi105 – 1051P → A: No effect on channel opening. 1 Publication
Mutagenesisi107 – 1071D → A: Strongly interferes with voltage-sensitive channel opening. 1 Publication
Mutagenesisi108 – 1081I → A: No effect on channel opening. 1 Publication
Mutagenesisi111 – 1111E → A: Interferes with voltage-sensitive channel opening. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Potassium voltage-gated channel subfamily A member 2
PRO_0000053975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi207 – 2071N-linked (GlcNAc...) Reviewed prediction
Lipidationi244 – 2441S-palmitoyl cysteine Reviewed prediction
Modified residuei429 – 4291Phosphotyrosine By similarity
Modified residuei449 – 4491Phosphoserine; by PKA Reviewed prediction

Post-translational modificationi

Phosphorylated on tyrosine residues by activated PTK2B/PYK2; this regulates ion channel activity.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP63142.
PRIDEiP63142.

PTM databases

PhosphoSiteiP63142.

Expressioni

Gene expression databases

GenevestigatoriP63142.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG4P783522EBI-631446,EBI-80389From a different organism.

Protein-protein interaction databases

BioGridi247501. 5 interactions.
IntActiP63142. 1 interaction.
STRINGi10116.ENSRNOP00000042653.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 396
Beta strandi42 – 476
Helixi48 – 525
Turni58 – 603
Helixi62 – 665
Turni71 – 744
Beta strandi75 – 784
Turni82 – 843
Helixi85 – 939
Helixi106 – 11611
Helixi120 – 13011
Turni143 – 1453
Helixi146 – 1494
Turni150 – 1545
Beta strandi156 – 1583
Helixi160 – 18223
Helixi186 – 1894
Beta strandi190 – 1923
Turni193 – 1964
Helixi202 – 2109
Helixi221 – 24121
Turni249 – 2524
Helixi254 – 2618
Helixi264 – 2674
Helixi268 – 2703
Beta strandi273 – 2775
Helixi279 – 2824
Helixi291 – 2999
Helixi304 – 3096
Helixi312 – 32312
Helixi325 – 35127
Helixi361 – 37212
Beta strandi378 – 3803
Helixi385 – 40319
Helixi406 – 41813

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DSXX-ray1.60A/B/C/D/E/F/G/H33-119[»]
1QDVX-ray1.60A/B/C/D33-131[»]
1QDWX-ray2.10A/B/C/D/E/F/G/H33-119[»]
2A79X-ray2.90B1-499[»]
2R9RX-ray2.40B/H1-499[»]
3LNMX-ray2.90B/D1-266[»]
B/D303-499[»]
3LUTX-ray2.90B1-499[»]
4JTAX-ray2.50B/Q1-499[»]
4JTCX-ray2.56B/H1-266[»]
B/H304-499[»]
4JTDX-ray2.54B/H1-266[»]
B/H304-499[»]
ProteinModelPortaliP63142.
SMRiP63142. Positions 3-421.

Miscellaneous databases

EvolutionaryTraceiP63142.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi374 – 3796Selectivity filter
Motifi497 – 4993PDZ-binding

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00740000115256.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP63142.
KOiK04875.
OMAiMTFHTYS.
OrthoDBiEOG7M0NRD.
PhylomeDBiP63142.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004049. K_chnl_volt-dep_Kv1.2.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01509. KV12CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P63142-1 [UniParc]FASTAAdd to Basket

« Hide

MTVATGDPVD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT    50
LAQFPETLLG DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR 100
PVNVPLDIFS EEIRFYELGE EAMEMFREDE GYIKEEERPL PENEFQRQVW 150
LLFEYPESSG PARIIAIVSV MVILISIVSF CLETLPIFRD ENEDMHGGGV 200
TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR FFACPSKAGF 250
FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLVR 300
VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE 350
ADERDSQFPS IPDAFWWAVV SMTTVGYGDM VPTTIGGKIV GSLCAIAGVL 400
TIALPVPVIV SNFNYFYHRE TEGEEQAQYL QVTSCPKIPS SPDLKKSRSA 450
STISKSDYME IQEGVNNSNE DFREENLKTA NCTLANTNYV NITKMLTDV 499
Length:499
Mass (Da):56,701
Last modified:September 13, 2004 - v1
Checksum:iA8FEA6F3F59AF42A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti411 – 4111S → F in AAA19867. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04731 mRNA. Translation: AAA40819.1.
X16003 mRNA. Translation: CAA34134.1.
M74449 mRNA. Translation: AAA19867.1.
PIRiA33814.
RefSeqiNP_037102.1. NM_012970.3.
XP_006233194.1. XM_006233132.1.
XP_006233195.1. XM_006233133.1.
XP_006233196.1. XM_006233134.1.
XP_006233197.1. XM_006233135.1.
UniGeneiRn.10298.
Rn.40779.

Genome annotation databases

EnsembliENSRNOT00000050149; ENSRNOP00000042653; ENSRNOG00000018285.
GeneIDi25468.
KEGGirno:25468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04731 mRNA. Translation: AAA40819.1 .
X16003 mRNA. Translation: CAA34134.1 .
M74449 mRNA. Translation: AAA19867.1 .
PIRi A33814.
RefSeqi NP_037102.1. NM_012970.3.
XP_006233194.1. XM_006233132.1.
XP_006233195.1. XM_006233133.1.
XP_006233196.1. XM_006233134.1.
XP_006233197.1. XM_006233135.1.
UniGenei Rn.10298.
Rn.40779.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DSX X-ray 1.60 A/B/C/D/E/F/G/H 33-119 [» ]
1QDV X-ray 1.60 A/B/C/D 33-131 [» ]
1QDW X-ray 2.10 A/B/C/D/E/F/G/H 33-119 [» ]
2A79 X-ray 2.90 B 1-499 [» ]
2R9R X-ray 2.40 B/H 1-499 [» ]
3LNM X-ray 2.90 B/D 1-266 [» ]
B/D 303-499 [» ]
3LUT X-ray 2.90 B 1-499 [» ]
4JTA X-ray 2.50 B/Q 1-499 [» ]
4JTC X-ray 2.56 B/H 1-266 [» ]
B/H 304-499 [» ]
4JTD X-ray 2.54 B/H 1-266 [» ]
B/H 304-499 [» ]
ProteinModelPortali P63142.
SMRi P63142. Positions 3-421.
ModBasei Search...

Protein-protein interaction databases

BioGridi 247501. 5 interactions.
IntActi P63142. 1 interaction.
STRINGi 10116.ENSRNOP00000042653.

Chemistry

GuidetoPHARMACOLOGYi 539.

PTM databases

PhosphoSitei P63142.

Proteomic databases

PaxDbi P63142.
PRIDEi P63142.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000050149 ; ENSRNOP00000042653 ; ENSRNOG00000018285 .
GeneIDi 25468.
KEGGi rno:25468.

Organism-specific databases

CTDi 3737.
RGDi 2950. Kcna2.

Phylogenomic databases

eggNOGi COG1226.
GeneTreei ENSGT00740000115256.
HOGENOMi HOG000231015.
HOVERGENi HBG052230.
InParanoidi P63142.
KOi K04875.
OMAi MTFHTYS.
OrthoDBi EOG7M0NRD.
PhylomeDBi P63142.
TreeFami TF313103.

Enzyme and pathway databases

Reactomei REACT_199159. Voltage gated Potassium channels.

Miscellaneous databases

EvolutionaryTracei P63142.
NextBioi 289787.
PROi P63142.

Gene expression databases

Genevestigatori P63142.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004049. K_chnl_volt-dep_Kv1.2.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01509. KV12CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family."
    McKinnon D.
    J. Biol. Chem. 264:8230-8236(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain."
    Stuehmer W., Ruppersberg J.P., Schroerter K.H., Sakmann B., Stocker M., Giese K.P., Perschke A., Baumann A., Pongs O.
    EMBO J. 8:3235-3244(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. Ludwig J.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart atrium.
  5. "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."
    Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M.
    Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
  6. "Protein tyrosine kinase PYK2 involved in Ca(2+)-induced regulation of ion channel and MAP kinase functions."
    Lev S., Moreno H., Martinez R., Canoll P., Peles E., Musacchio J.M., Plowman G.D., Rudy B., Schlessinger J.
    Nature 376:737-745(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
    Tissue: Brain.
  7. "The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel."
    Minor D.L. Jr., Lin Y.-F., Mobley B.C., Avelar A., Jan Y.N., Jan L.Y., Berger J.M.
    Cell 102:657-670(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 33-119, TETRAMERIZATION, MUTAGENESIS OF ARG-34; ASN-38; SER-40; GLY-41; LEU-42; ARG-43; PHE-44; GLU-45; THR-46; GLN-47; THR-50; ASP-70; ARG-73; GLU-75; PHE-77; ASP-79; ASN-81; ARG-82; ASP-86; LEU-89; TYR-90; GLN-93; ARG-97; ARG-99; VAL-102; ASN-103; PRO-105; ASP-107; ILE-108 AND GLU-111.
  8. "Crystal structure of a mammalian voltage-dependent Shaker family K+ channel."
    Long S.B., Campbell E.B., Mackinnon R.
    Science 309:897-903(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH KCNAB2, SUBUNIT.

Entry informationi

Entry nameiKCNA2_RAT
AccessioniPrimary (citable) accession number: P63142
Secondary accession number(s): P15386, Q02010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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