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P63141

- KCNA2_MOUSE

UniProt

P63141 - KCNA2_MOUSE

Protein

Potassium voltage-gated channel subfamily A member 2

Gene

Kcna2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Regulates neuronal excitability and is involved in non-rapid eye movement (NREM) sleep.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome
    2. outward rectifier potassium channel activity Source: Ensembl
    3. protein binding Source: IntAct

    GO - Biological processi

    1. optic nerve structural organization Source: MGI
    2. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199077. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 2
    Alternative name(s):
    MK2
    Voltage-gated potassium channel subunit Kv1.2
    Gene namesi
    Name:Kcna2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:96659. Kcna2.

    Subcellular locationi

    GO - Cellular componenti

    1. juxtaparanode region of axon Source: BHF-UCL
    2. voltage-gated potassium channel complex Source: BHF-UCL

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Death by P28 due to generalized seizures. Pups display less non-rapid eye movement (NREM) sleep and significantly more waking periods. Mice appear healthy and develop normally during the first 2 weeks, but die suddenly between around P12 and P28 due to an episode of generalized seizure, followed by full tonic extension, which in mice often results in fatal apne. At P17 seizures are either absent or very rare and abnormal electroencephalograph activity is only present during the seizure. P17 pups have significantly less non-rapid NREM sleep (-23%) and significantly more waking (+21%) than wild-type siblings with no change in rapid eye movement (REM) sleep time. The decrease in NREM sleep is due to an increase in the number of waking episodes, with no change in number or duration of sleep episodes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Potassium voltage-gated channel subfamily A member 2PRO_0000053973Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
    Lipidationi244 – 2441S-palmitoyl cysteineSequence Analysis
    Modified residuei429 – 4291Phosphotyrosine1 Publication
    Modified residuei449 – 4491Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    Phosphorylated on tyrosine residues by activated PTK2B/PYK2; this regulates ion channel activity.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP63141.
    PRIDEiP63141.

    Expressioni

    Gene expression databases

    ArrayExpressiP63141.
    BgeeiP63141.
    GenevestigatoriP63141.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Sigmar1O552423EBI-644033,EBI-1557700
    Sigmar1Q9R0C93EBI-644033,EBI-1557826From a different organism.

    Protein-protein interaction databases

    BioGridi200877. 3 interactions.
    DIPiDIP-32239N.
    IntActiP63141. 5 interactions.
    MINTiMINT-1659109.

    Structurei

    3D structure databases

    ProteinModelPortaliP63141.
    SMRiP63141. Positions 3-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei164 – 18219Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei222 – 24322Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei293 – 31119Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei328 – 34720Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei389 – 41123Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi374 – 3796Selectivity filterBy similarity
    Motifi497 – 4993PDZ-bindingBy similarity

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115256.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiP63141.
    KOiK04875.
    OMAiMTFHTYS.
    OrthoDBiEOG7M0NRD.
    PhylomeDBiP63141.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004049. K_chnl_volt-dep_Kv1.2.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01509. KV12CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P63141-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVATGDPVD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT    50
    LAQFPETLLG DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR 100
    PVNVPLDIFS EEIRFYELGE EAMEMFREDE GYIKEEERPL PENEFQRQVW 150
    LLFEYPESSG PARIIAIVSV MVILISIVSF CLETLPIFRD ENEDMHGGGV 200
    TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR FFACPSKAGF 250
    FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLVR 300
    VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE 350
    ADERDSQFPS IPDAFWWAVV SMTTVGYGDM VPTTIGGKIV GSLCAIAGVL 400
    TIALPVPVIV SNFNYFYHRE TEGEEQAQYL QVTSCPKIPS SPDLKKSRSA 450
    STISKSDYME IQEGVNNSNE DFREENLKTA NCTLANTNYV NITKMLTDV 499
    Length:499
    Mass (Da):56,701
    Last modified:September 13, 2004 - v1
    Checksum:iA8FEA6F3F59AF42A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331E → G in BAC31877. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30440 Genomic DNA. Translation: AAA39713.1.
    AK044342 mRNA. Translation: BAC31877.1.
    CCDSiCCDS17733.1.
    PIRiB40090. I84204.
    RefSeqiNP_032443.3. NM_008417.5.
    XP_006501111.1. XM_006501048.1.
    XP_006501112.1. XM_006501049.1.
    XP_006501113.1. XM_006501050.1.
    XP_006501114.1. XM_006501051.1.
    XP_006501115.1. XM_006501052.1.
    XP_006501116.1. XM_006501053.1.
    XP_006501117.1. XM_006501054.1.
    XP_006501118.1. XM_006501055.1.
    UniGeneiMm.39285.

    Genome annotation databases

    EnsembliENSMUST00000038695; ENSMUSP00000041702; ENSMUSG00000040724.
    GeneIDi16490.
    KEGGimmu:16490.
    UCSCiuc008qws.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30440 Genomic DNA. Translation: AAA39713.1 .
    AK044342 mRNA. Translation: BAC31877.1 .
    CCDSi CCDS17733.1.
    PIRi B40090. I84204.
    RefSeqi NP_032443.3. NM_008417.5.
    XP_006501111.1. XM_006501048.1.
    XP_006501112.1. XM_006501049.1.
    XP_006501113.1. XM_006501050.1.
    XP_006501114.1. XM_006501051.1.
    XP_006501115.1. XM_006501052.1.
    XP_006501116.1. XM_006501053.1.
    XP_006501117.1. XM_006501054.1.
    XP_006501118.1. XM_006501055.1.
    UniGenei Mm.39285.

    3D structure databases

    ProteinModelPortali P63141.
    SMRi P63141. Positions 3-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200877. 3 interactions.
    DIPi DIP-32239N.
    IntActi P63141. 5 interactions.
    MINTi MINT-1659109.

    Proteomic databases

    PaxDbi P63141.
    PRIDEi P63141.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038695 ; ENSMUSP00000041702 ; ENSMUSG00000040724 .
    GeneIDi 16490.
    KEGGi mmu:16490.
    UCSCi uc008qws.2. mouse.

    Organism-specific databases

    CTDi 3737.
    MGIi MGI:96659. Kcna2.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115256.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi P63141.
    KOi K04875.
    OMAi MTFHTYS.
    OrthoDBi EOG7M0NRD.
    PhylomeDBi P63141.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199077. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 289787.
    PROi P63141.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63141.
    Bgeei P63141.
    Genevestigatori P63141.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004049. K_chnl_volt-dep_Kv1.2.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01509. KV12CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A family of three mouse potassium channel genes with intronless coding regions."
      Chandy K.G., Williams C.B., Spencer R.H., Aguilar B.A., Ghanshani S., Tempel B.L., Gutman G.A.
      Science 247:973-975(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Retina.
    3. "Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction."
      Betsholtz C., Baumann A., Kenna S., Ashcroft F.M., Ashcroft S.J.H., Berggren P.-O., Grupe A., Pongs O., Rorsman P., Sandblom J., Welsh M.
      FEBS Lett. 263:121-126(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-394.
    4. Cited for: DISRUPTION PHENOTYPE.
    5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNA2_MOUSE
    AccessioniPrimary (citable) accession number: P63141
    Secondary accession number(s): P15386, Q02010, Q8C8W4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3