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P63141

- KCNA2_MOUSE

UniProt

P63141 - KCNA2_MOUSE

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Protein

Potassium voltage-gated channel subfamily A member 2

Gene

Kcna2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Regulates neuronal excitability and is involved in non-rapid eye movement (NREM) sleep.

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: RefGenome
  2. outward rectifier potassium channel activity Source: Ensembl

GO - Biological processi

  1. optic nerve structural organization Source: MGI
  2. protein homooligomerization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199077. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 2
Alternative name(s):
MK2
Voltage-gated potassium channel subunit Kv1.2
Gene namesi
Name:Kcna2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:96659. Kcna2.

Subcellular locationi

GO - Cellular componenti

  1. juxtaparanode region of axon Source: BHF-UCL
  2. voltage-gated potassium channel complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Death by P28 due to generalized seizures. Pups display less non-rapid eye movement (NREM) sleep and significantly more waking periods. Mice appear healthy and develop normally during the first 2 weeks, but die suddenly between around P12 and P28 due to an episode of generalized seizure, followed by full tonic extension, which in mice often results in fatal apne. At P17 seizures are either absent or very rare and abnormal electroencephalograph activity is only present during the seizure. P17 pups have significantly less non-rapid NREM sleep (-23%) and significantly more waking (+21%) than wild-type siblings with no change in rapid eye movement (REM) sleep time. The decrease in NREM sleep is due to an increase in the number of waking episodes, with no change in number or duration of sleep episodes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Potassium voltage-gated channel subfamily A member 2PRO_0000053973Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
Lipidationi244 – 2441S-palmitoyl cysteineSequence Analysis
Modified residuei429 – 4291Phosphotyrosine1 Publication
Modified residuei449 – 4491Phosphoserine; by PKASequence Analysis

Post-translational modificationi

Phosphorylated on tyrosine residues by activated PTK2B/PYK2; this regulates ion channel activity.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP63141.
PRIDEiP63141.

Expressioni

Gene expression databases

BgeeiP63141.
ExpressionAtlasiP63141. baseline and differential.
GenevestigatoriP63141.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Sigmar1O552423EBI-644033,EBI-1557700
Sigmar1Q9R0C93EBI-644033,EBI-1557826From a different organism.

Protein-protein interaction databases

BioGridi200877. 3 interactions.
DIPiDIP-32239N.
IntActiP63141. 5 interactions.
MINTiMINT-1659109.

Structurei

3D structure databases

ProteinModelPortaliP63141.
SMRiP63141. Positions 3-421.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei164 – 18219Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei222 – 24322Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Transmembranei255 – 27521Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei293 – 31119Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Transmembranei328 – 34720Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Transmembranei389 – 41123Helical; Name=Segment S6Sequence AnalysisAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi374 – 3796Selectivity filterBy similarity
Motifi497 – 4993PDZ-bindingBy similarity

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP63141.
KOiK04875.
OMAiMTFHTYS.
OrthoDBiEOG7M0NRD.
PhylomeDBiP63141.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004049. K_chnl_volt-dep_Kv1.2.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01509. KV12CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P63141 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVATGDPVD EAAALPGHPQ DTYDPEADHE CCERVVINIS GLRFETQLKT
60 70 80 90 100
LAQFPETLLG DPKKRMRYFD PLRNEYFFDR NRPSFDAILY YYQSGGRLRR
110 120 130 140 150
PVNVPLDIFS EEIRFYELGE EAMEMFREDE GYIKEEERPL PENEFQRQVW
160 170 180 190 200
LLFEYPESSG PARIIAIVSV MVILISIVSF CLETLPIFRD ENEDMHGGGV
210 220 230 240 250
TFHTYSNSTI GYQQSTSFTD PFFIVETLCI IWFSFEFLVR FFACPSKAGF
260 270 280 290 300
FTNIMNIIDI VAIIPYFITL GTELAEKPED AQQGQQAMSL AILRVIRLVR
310 320 330 340 350
VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE
360 370 380 390 400
ADERDSQFPS IPDAFWWAVV SMTTVGYGDM VPTTIGGKIV GSLCAIAGVL
410 420 430 440 450
TIALPVPVIV SNFNYFYHRE TEGEEQAQYL QVTSCPKIPS SPDLKKSRSA
460 470 480 490
STISKSDYME IQEGVNNSNE DFREENLKTA NCTLANTNYV NITKMLTDV
Length:499
Mass (Da):56,701
Last modified:September 13, 2004 - v1
Checksum:iA8FEA6F3F59AF42A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331E → G in BAC31877. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30440 Genomic DNA. Translation: AAA39713.1.
AK044342 mRNA. Translation: BAC31877.1.
CCDSiCCDS17733.1.
PIRiB40090. I84204.
RefSeqiNP_032443.3. NM_008417.5.
XP_006501111.1. XM_006501048.1.
XP_006501112.1. XM_006501049.1.
XP_006501113.1. XM_006501050.1.
XP_006501114.1. XM_006501051.1.
XP_006501115.1. XM_006501052.1.
XP_006501116.1. XM_006501053.1.
XP_006501117.1. XM_006501054.1.
XP_006501118.1. XM_006501055.1.
UniGeneiMm.39285.

Genome annotation databases

EnsembliENSMUST00000038695; ENSMUSP00000041702; ENSMUSG00000040724.
GeneIDi16490.
KEGGimmu:16490.
UCSCiuc008qws.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30440 Genomic DNA. Translation: AAA39713.1 .
AK044342 mRNA. Translation: BAC31877.1 .
CCDSi CCDS17733.1.
PIRi B40090. I84204.
RefSeqi NP_032443.3. NM_008417.5.
XP_006501111.1. XM_006501048.1.
XP_006501112.1. XM_006501049.1.
XP_006501113.1. XM_006501050.1.
XP_006501114.1. XM_006501051.1.
XP_006501115.1. XM_006501052.1.
XP_006501116.1. XM_006501053.1.
XP_006501117.1. XM_006501054.1.
XP_006501118.1. XM_006501055.1.
UniGenei Mm.39285.

3D structure databases

ProteinModelPortali P63141.
SMRi P63141. Positions 3-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200877. 3 interactions.
DIPi DIP-32239N.
IntActi P63141. 5 interactions.
MINTi MINT-1659109.

Proteomic databases

PaxDbi P63141.
PRIDEi P63141.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000038695 ; ENSMUSP00000041702 ; ENSMUSG00000040724 .
GeneIDi 16490.
KEGGi mmu:16490.
UCSCi uc008qws.2. mouse.

Organism-specific databases

CTDi 3737.
MGIi MGI:96659. Kcna2.

Phylogenomic databases

eggNOGi COG1226.
GeneTreei ENSGT00760000118846.
HOGENOMi HOG000231015.
HOVERGENi HBG052230.
InParanoidi P63141.
KOi K04875.
OMAi MTFHTYS.
OrthoDBi EOG7M0NRD.
PhylomeDBi P63141.
TreeFami TF313103.

Enzyme and pathway databases

Reactomei REACT_199077. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi 289787.
PROi P63141.
SOURCEi Search...

Gene expression databases

Bgeei P63141.
ExpressionAtlasi P63141. baseline and differential.
Genevestigatori P63141.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004049. K_chnl_volt-dep_Kv1.2.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01509. KV12CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A family of three mouse potassium channel genes with intronless coding regions."
    Chandy K.G., Williams C.B., Spencer R.H., Aguilar B.A., Ghanshani S., Tempel B.L., Gutman G.A.
    Science 247:973-975(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Retina.
  3. "Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction."
    Betsholtz C., Baumann A., Kenna S., Ashcroft F.M., Ashcroft S.J.H., Berggren P.-O., Grupe A., Pongs O., Rorsman P., Sandblom J., Welsh M.
    FEBS Lett. 263:121-126(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-394.
  4. Cited for: DISRUPTION PHENOTYPE.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiKCNA2_MOUSE
AccessioniPrimary (citable) accession number: P63141
Secondary accession number(s): P15386, Q02010, Q8C8W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3