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Protein

Nuclear transcription factor Y subunit beta

Gene

Nfyb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi59 – 65By similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit beta
Alternative name(s):
CAAT box DNA-binding protein subunit B
CCAAT-binding transcription factor subunit B
Short name:
CBF-B
Nuclear transcription factor Y subunit B
Short name:
NF-YB
Gene namesi
Name:Nfyb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3172. Nfyb.

Subcellular locationi

GO - Cellular componenti

  • CCAAT-binding factor complex Source: RGD
  • protein-DNA complex Source: Ensembl

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002046111 – 207Nuclear transcription factor Y subunit betaAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki140Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP63140.
PRIDEiP63140.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010309.
ExpressionAtlasiP63140. baseline and differential.
GenevisibleiP63140. RN.

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with C1QBP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-2660N.
STRINGi10116.ENSRNOP00000061405.

Structurei

3D structure databases

ProteinModelPortaliP63140.
SMRiP63140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 52A domainAdd BLAST52
Regioni53 – 142B domainAdd BLAST90
Regioni86 – 97Subunit association domain (SAD)By similarityAdd BLAST12
Regioni143 – 207C domainAdd BLAST65

Domaini

Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.Curated

Phylogenomic databases

eggNOGiKOG0869. Eukaryota.
COG2036. LUCA.
GeneTreeiENSGT00550000074689.
HOGENOMiHOG000211832.
HOVERGENiHBG008864.
InParanoidiP63140.
KOiK08065.
OMAiKENFREQ.
PhylomeDBiP63140.
TreeFamiTF314521.

Family and domain databases

InterProiView protein in InterPro
IPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
PfamiView protein in Pfam
PF00808. CBFD_NFYB_HMF. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiView protein in PROSITE
PS00685. NFYB_HAP3. 1 hit.

Sequencei

Sequence statusi: Complete.

P63140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE
60 70 80 90 100
SFREQDIYLP IANVARIMKN AIPQTGKIAK DAKECVQECV SEFISFITSE
110 120 130 140 150
ASERCHQEKR KTINGEDILF AMSTLGFDSY VEPLKLYLQK FREAMKGEKG
160 170 180 190 200
IGGAVSATDG LSEELTEEAF TNQLPAGLIT ADGQQQNVMV YTTSYQQISG

VQQIQFS
Length:207
Mass (Da):22,787
Last modified:September 13, 2004 - v1
Checksum:i1ADFB4B04A3CFC22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55045 mRNA. Translation: AAA40887.1.
BC089791 mRNA. Translation: AAH89791.1.
PIRiA23692.
RefSeqiNP_113741.1. NM_031553.2.
XP_006241258.1. XM_006241196.3.
UniGeneiRn.90077.

Genome annotation databases

EnsembliENSRNOT00000066143; ENSRNOP00000061405; ENSRNOG00000010309.
GeneIDi25336.
KEGGirno:25336.
UCSCiRGD:3172. rat.

Similar proteinsi

Entry informationi

Entry nameiNFYB_RAT
AccessioniPrimary (citable) accession number: P63140
Secondary accession number(s): P22569, Q5FVT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: August 30, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families