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P63139

- NFYB_MOUSE

UniProt

P63139 - NFYB_MOUSE

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Protein

Nuclear transcription factor Y subunit beta

Gene

Nfyb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi59 – 657By similarity

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. positive regulation of transcription, DNA-templated Source: MGI
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_198969. Activation of gene expression by SREBF (SREBP).
REACT_32838. ATF6-alpha activates chaperone genes.
REACT_90370. ATF4 activates genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit beta
Alternative name(s):
CAAT box DNA-binding protein subunit B
Nuclear transcription factor Y subunit B
Short name:
NF-YB
Gene namesi
Name:Nfyb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:97317. Nfyb.

Subcellular locationi

GO - Cellular componenti

  1. CCAAT-binding factor complex Source: Ensembl
  2. nucleoplasm Source: Reactome
  3. nucleus Source: MGI
  4. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Nuclear transcription factor Y subunit betaPRO_0000204610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki140 – 140Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP63139.
PRIDEiP63139.

PTM databases

PhosphoSiteiP63139.

Expressioni

Gene expression databases

BgeeiP63139.
CleanExiMM_NFYB.
ExpressionAtlasiP63139. baseline and differential.
GenevestigatoriP63139.

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with C1QBP (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP63139.
SMRiP63139. Positions 57-143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5252A domainAdd
BLAST
Regioni53 – 14290B domainAdd
BLAST
Regioni86 – 9712Subunit association domain (SAD)By similarityAdd
BLAST
Regioni143 – 20765C domainAdd
BLAST

Domaini

Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.Curated

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00550000074689.
HOVERGENiHBG008864.
InParanoidiP63139.
KOiK08065.
OMAiIAGDYIG.
OrthoDBiEOG7RNK2W.
TreeFamiTF314521.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00685. NFYB_HAP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: 3 isoforms may be produced.

Isoform 1 (identifier: P63139-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE
60 70 80 90 100
SFREQDIYLP IANVARIMKN AIPQTGKIAK DAKECVQECV SEFISFITSE
110 120 130 140 150
ASERCHQEKR KTINGEDILF AMSTLGFDSY VEPLKLYLQK FREAMKGEKG
160 170 180 190 200
IGGAVSATDG LSEELTEEAF TNQLPAGLIT ADGQQQNVMV YTTSYQQISG

VQQIQFS
Length:207
Mass (Da):22,787
Last modified:September 13, 2004 - v1
Checksum:i1ADFB4B04A3CFC22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55316 mRNA. Translation: CAA39024.1.
AK010762 mRNA. Translation: BAB27166.1.
AK146168 mRNA. Translation: BAE26948.1.
BC010719 mRNA. Translation: AAH10719.1.
CCDSiCCDS48654.1.
PIRiF38245.
RefSeqiNP_035044.1. NM_010914.2. [P63139-1]
UniGeneiMm.245998.

Genome annotation databases

EnsembliENSMUST00000130911; ENSMUSP00000122403; ENSMUSG00000020248. [P63139-1]
GeneIDi18045.
KEGGimmu:18045.
UCSCiuc007gjv.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55316 mRNA. Translation: CAA39024.1 .
AK010762 mRNA. Translation: BAB27166.1 .
AK146168 mRNA. Translation: BAE26948.1 .
BC010719 mRNA. Translation: AAH10719.1 .
CCDSi CCDS48654.1.
PIRi F38245.
RefSeqi NP_035044.1. NM_010914.2. [P63139-1 ]
UniGenei Mm.245998.

3D structure databases

ProteinModelPortali P63139.
SMRi P63139. Positions 57-143.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P63139.

Proteomic databases

MaxQBi P63139.
PRIDEi P63139.

Protocols and materials databases

DNASUi 18045.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000130911 ; ENSMUSP00000122403 ; ENSMUSG00000020248 . [P63139-1 ]
GeneIDi 18045.
KEGGi mmu:18045.
UCSCi uc007gjv.1. mouse.

Organism-specific databases

CTDi 4801.
MGIi MGI:97317. Nfyb.

Phylogenomic databases

eggNOGi COG2036.
GeneTreei ENSGT00550000074689.
HOVERGENi HBG008864.
InParanoidi P63139.
KOi K08065.
OMAi IAGDYIG.
OrthoDBi EOG7RNK2W.
TreeFami TF314521.

Enzyme and pathway databases

Reactomei REACT_198602. PPARA activates gene expression.
REACT_198969. Activation of gene expression by SREBF (SREBP).
REACT_32838. ATF6-alpha activates chaperone genes.
REACT_90370. ATF4 activates genes.

Miscellaneous databases

NextBioi 293161.
PROi P63139.
SOURCEi Search...

Gene expression databases

Bgeei P63139.
CleanExi MM_NFYB.
ExpressionAtlasi P63139. baseline and differential.
Genevestigatori P63139.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view ]
Pfami PF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
PROSITEi PS00685. NFYB_HAP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Co-evolution from yeast to mouse: cDNA cloning of the two NF-Y (CP-1/CBF) subunits."
    Hooft van Huijsduijnen R., Li X.-Y., Black D., Matthes H., Benoist C., Mathis D.
    EMBO J. 9:3119-3127(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-69; 137-140 AND 194-206.
  2. "Evolutionary variation of the CCAAT-binding transcription factor NF-Y."
    Li X.-Y., Mantovani R., Hooft van Huijsduijnen R., Andre I., Benoist C., Mathis D.
    Nucleic Acids Res. 20:1087-1091(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain."
    Li X.-Y., Hooft van Huijsduijnen R., Mantovani R., Benoist C.O., Mathis D.
    J. Biol. Chem. 267:8984-8990(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BALB/c and C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiNFYB_MOUSE
AccessioniPrimary (citable) accession number: P63139
Secondary accession number(s): P22569, Q3UK54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3