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Protein

Gamma-aminobutyric acid receptor subunit beta-2

Gene

Gabrb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241AgonistBy similarity

GO - Molecular functioni

  • chloride channel activity Source: UniProtKB
  • GABA-A receptor activity Source: UniProtKB
  • inhibitory extracellular ligand-gated ion channel activity Source: RGD

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chemical synaptic transmission Source: Reactome
  • chloride transmembrane transport Source: UniProtKB
  • cochlea development Source: Ensembl
  • gamma-aminobutyric acid signaling pathway Source: RGD
  • inner ear receptor cell development Source: Ensembl
  • innervation Source: Ensembl
  • negative regulation of neuron apoptotic process Source: Ensembl
  • sensory perception of sound Source: Ensembl
  • synaptic transmission, GABAergic Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-112314. Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell.
R-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-2
Alternative name(s):
GABA(A) receptor subunit beta-2
Gene namesi
Name:Gabrb2
Synonyms:Gabrb-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2650. Gabrb2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 244219ExtracellularCuratedAdd
BLAST
Transmembranei245 – 26622HelicalCuratedAdd
BLAST
Transmembranei270 – 29223HelicalCuratedAdd
BLAST
Transmembranei304 – 32623HelicalCuratedAdd
BLAST
Topological domaini327 – 451125CytoplasmicCuratedAdd
BLAST
Transmembranei452 – 47322HelicalCuratedAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • cytosol Source: RGD
  • extracellular exosome Source: Ensembl
  • GABA-A receptor complex Source: UniProtKB
  • GABA receptor complex Source: BHF-UCL
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: RGD
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907607.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 474449Gamma-aminobutyric acid receptor subunit beta-2PRO_0000000461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence analysis
Cross-linki45 – 45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence analysis
Disulfide bondi160 ↔ 174By similarity
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence analysis
Modified residuei403 – 4031PhosphotyrosineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP63138.
PRIDEiP63138.

PTM databases

iPTMnetiP63138.
PhosphoSiteiP63138.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003680.
GenevisibleiP63138. RN.

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Binds UBQLN1. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents.3 Publications

Protein-protein interaction databases

IntActiP63138. 2 interactions.
STRINGi10116.ENSRNOP00000004970.

Chemistry

BindingDBiP63138.

Structurei

3D structure databases

ProteinModelPortaliP63138.
SMRiP63138. Positions 59-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 1213Agonist bindingBy similarity
Regioni179 – 1813Agonist bindingBy similarity
Regioni289 – 31022Allosteric effector bindingBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63138.
KOiK05181.
OMAiHMTQKKS.
OrthoDBiEOG091G0805.
PhylomeDBiP63138.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWRVRKRGYF GIWSFPLIIA AVCAQSVNDP SNMSLVKETV DRLLKGYDIR
60 70 80 90 100
LRPDFGGPPV AVGMNIDIAS IDMVSEVNMD YTLTMYFQQA WRDKRLSYNV
110 120 130 140 150
IPLNLTLDNR VADQLWVPDT YFLNDKKSFV HGVTVKNRMI RLHPDGTVLY
160 170 180 190 200
GLRITTTAAC MMDLRRYPLD EQNCTLEIES YGYTTDDIEF YWRGDDNAVT
210 220 230 240 250
GVTKIELPQF SIVDYKLITK KVVFSTGSYP RLSLSFKLKR NIGYFILQTY
260 270 280 290 300
MPSILITILS WVSFWINYDA SAARVALGIT TVLTMTTINT HLRETLPKIP
310 320 330 340 350
YVKAIDMYLM GCFVFVFMAL LEYALVNYIF FGRGPQRQKK AAEKAANANN
360 370 380 390 400
EKMRLDVNKM DPHENILLST LEIKNEMATS EAVMGLGDPR STMLAYDASS
410 420 430 440 450
IQYRKAGLPR HSFGRNALER HVAQKKSRLR RRASQLKITI PDLTDVNAID
460 470
RWSRIFFPVV FSFFNIVYWL YYVN
Length:474
Mass (Da):54,633
Last modified:September 13, 2004 - v1
Checksum:i8A815982EB70933E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15467 mRNA. Translation: CAA33494.1.
AY574251 mRNA. Translation: AAS90347.1.
PIRiS04465.
RefSeqiNP_037089.1. NM_012957.2.
XP_006246189.1. XM_006246127.2.
UniGeneiRn.207158.
Rn.24387.

Genome annotation databases

EnsembliENSRNOT00000004970; ENSRNOP00000004970; ENSRNOG00000003680.
GeneIDi25451.
KEGGirno:25451.
UCSCiRGD:2650. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15467 mRNA. Translation: CAA33494.1.
AY574251 mRNA. Translation: AAS90347.1.
PIRiS04465.
RefSeqiNP_037089.1. NM_012957.2.
XP_006246189.1. XM_006246127.2.
UniGeneiRn.207158.
Rn.24387.

3D structure databases

ProteinModelPortaliP63138.
SMRiP63138. Positions 59-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP63138. 2 interactions.
STRINGi10116.ENSRNOP00000004970.

Chemistry

BindingDBiP63138.
ChEMBLiCHEMBL1907607.

PTM databases

iPTMnetiP63138.
PhosphoSiteiP63138.

Proteomic databases

PaxDbiP63138.
PRIDEiP63138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004970; ENSRNOP00000004970; ENSRNOG00000003680.
GeneIDi25451.
KEGGirno:25451.
UCSCiRGD:2650. rat.

Organism-specific databases

CTDi2561.
RGDi2650. Gabrb2.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63138.
KOiK05181.
OMAiHMTQKKS.
OrthoDBiEOG091G0805.
PhylomeDBiP63138.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-RNO-112314. Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell.
R-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Miscellaneous databases

PROiP63138.

Gene expression databases

BgeeiENSRNOG00000003680.
GenevisibleiP63138. RN.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBRB2_RAT
AccessioniPrimary (citable) accession number: P63138
Secondary accession number(s): P15432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.