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Protein

Gamma-aminobutyric acid receptor subunit beta-2

Gene

Gabrb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241AgonistBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chloride transmembrane transport Source: UniProtKB
  • cochlea development Source: DFLAT
  • inner ear receptor cell development Source: DFLAT
  • innervation Source: DFLAT
  • negative regulation of neuron apoptotic process Source: DFLAT
  • negative regulation of neuron death Source: DFLAT
  • neuron development Source: DFLAT
  • regulation of neuron apoptotic process Source: DFLAT
  • sensory perception of sound Source: DFLAT
  • signal transduction Source: GOC
  • synaptic transmission, GABAergic Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-2
Alternative name(s):
GABA(A) receptor subunit beta-2
Gene namesi
Name:Gabrb2
Synonyms:Gabrb-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95620. Gabrb2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 244219ExtracellularCuratedAdd
BLAST
Transmembranei245 – 26622HelicalCuratedAdd
BLAST
Transmembranei270 – 29223HelicalCuratedAdd
BLAST
Transmembranei304 – 32623HelicalCuratedAdd
BLAST
Topological domaini327 – 489163CytoplasmicCuratedAdd
BLAST
Transmembranei490 – 51122HelicalCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 512487Gamma-aminobutyric acid receptor subunit beta-2PRO_0000000460Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence analysis
Cross-linki45 – 45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence analysis
Disulfide bondi160 ↔ 174By similarity
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence analysis
Modified residuei441 – 4411PhosphotyrosineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP63137.
PRIDEiP63137.

PTM databases

iPTMnetiP63137.
PhosphoSiteiP63137.

Expressioni

Gene expression databases

BgeeiP63137.
GenevisibleiP63137. MM.

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Binds UBQLN1 (By similarity). Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents.By similarity1 Publication

Protein-protein interaction databases

BioGridi199803. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP63137.
SMRiP63137. Positions 25-337, 486-512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 1213Agonist bindingBy similarity
Regioni179 – 1813Agonist bindingBy similarity
Regioni289 – 31022Allosteric effector bindingBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63137.
KOiK05181.
OMAiHMTQKKS.
OrthoDBiEOG712TVZ.
PhylomeDBiP63137.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P63137-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRVRKRGYF GIWSFPLIIA AVCAQSVNDP SNMSLVKETV DRLLKGYDIR
60 70 80 90 100
LRPDFGGPPV AVGMNIDIAS IDMVSEVNMD YTLTMYFQQA WRDKRLSYNV
110 120 130 140 150
IPLNLTLDNR VADQLWVPDT YFLNDKKSFV HGVTVKNRMI RLHPDGTVLY
160 170 180 190 200
GLRITTTAAC MMDLRRYPLD EQNCTLEIES YGYTTDDIEF YWRGDDNAVT
210 220 230 240 250
GVTKIELPQF SIVDYKLITK KVVFSTGSYP RLSLSFKLKR NIGYFILQTY
260 270 280 290 300
MPSILITILS WVSFWINYDA SAARVALGIT TVLTMTTINT HLRETLPKIP
310 320 330 340 350
YVKAIDMYLM GCFVFVFMAL LEYALVNYIF FGRGPQRQKK AAEKAANANN
360 370 380 390 400
EKMRLDVNKM FYKDIKQNGT QYRSLWDPTG DLSPTRRTTN YDFSLYTMDP
410 420 430 440 450
HENILLSTLE IKNEMATSEA VMGLGDPRST MLAYDASSIQ YRKAGLPRHS
460 470 480 490 500
FGRNALERHV AQKKSRLRRR ASQLKITIPD LTDVNAIDRW SRIFFPVVFS
510
FFNIVYWLYY VN
Length:512
Mass (Da):59,197
Last modified:March 23, 2010 - v2
Checksum:i41AC9154A438E4FF
GO
Isoform 2 (identifier: P63137-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     360-397: Missing.

Show »
Length:474
Mass (Da):54,633
Checksum:i8A815982EB70933E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei360 – 39738Missing in isoform 2. 3 PublicationsVSP_038829Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14419 mRNA. Translation: AAA79974.1.
GU086167 mRNA. Translation: ACY69098.1.
AK090279 mRNA. Translation: BAC41155.1.
AL627444, AL645964 Genomic DNA. Translation: CAI25055.1.
AL645964, AL627444 Genomic DNA. Translation: CAI25222.1.
CH466609 Genomic DNA. Translation: EDL32344.1.
BC145973 mRNA. Translation: AAI45974.1.
BC145975 mRNA. Translation: AAI45976.1.
CCDSiCCDS24555.1. [P63137-2]
PIRiS53531.
RefSeqiNP_032096.1. NM_008070.3. [P63137-2]
XP_011247028.1. XM_011248726.1. [P63137-1]
UniGeneiMm.338723.
Mm.471870.

Genome annotation databases

EnsembliENSMUST00000007797; ENSMUSP00000007797; ENSMUSG00000007653. [P63137-2]
ENSMUST00000192403; ENSMUSP00000141868; ENSMUSG00000007653. [P63137-1]
GeneIDi14401.
KEGGimmu:14401.
UCSCiuc007imi.1. mouse. [P63137-2]
uc011xte.1. mouse. [P63137-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14419 mRNA. Translation: AAA79974.1.
GU086167 mRNA. Translation: ACY69098.1.
AK090279 mRNA. Translation: BAC41155.1.
AL627444, AL645964 Genomic DNA. Translation: CAI25055.1.
AL645964, AL627444 Genomic DNA. Translation: CAI25222.1.
CH466609 Genomic DNA. Translation: EDL32344.1.
BC145973 mRNA. Translation: AAI45974.1.
BC145975 mRNA. Translation: AAI45976.1.
CCDSiCCDS24555.1. [P63137-2]
PIRiS53531.
RefSeqiNP_032096.1. NM_008070.3. [P63137-2]
XP_011247028.1. XM_011248726.1. [P63137-1]
UniGeneiMm.338723.
Mm.471870.

3D structure databases

ProteinModelPortaliP63137.
SMRiP63137. Positions 25-337, 486-512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199803. 2 interactions.

PTM databases

iPTMnetiP63137.
PhosphoSiteiP63137.

Proteomic databases

MaxQBiP63137.
PRIDEiP63137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007797; ENSMUSP00000007797; ENSMUSG00000007653. [P63137-2]
ENSMUST00000192403; ENSMUSP00000141868; ENSMUSG00000007653. [P63137-1]
GeneIDi14401.
KEGGimmu:14401.
UCSCiuc007imi.1. mouse. [P63137-2]
uc011xte.1. mouse. [P63137-1]

Organism-specific databases

CTDi2561.
MGIiMGI:95620. Gabrb2.

Phylogenomic databases

GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63137.
KOiK05181.
OMAiHMTQKKS.
OrthoDBiEOG712TVZ.
PhylomeDBiP63137.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Miscellaneous databases

ChiTaRSiGabrb2. mouse.
PROiP63137.
SOURCEiSearch...

Gene expression databases

BgeeiP63137.
GenevisibleiP63137. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GABAA receptor beta 1, beta 2, and beta 3 subunits: comparisons in DBA/2J and C57BL/6J mice."
    Kamatchi G.L., Kofuji P., Wang J.B., Fernando J.C., Liu Z., Mathura J.R., Burt D.R.
    Biochim. Biophys. Acta 1261:134-142(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6J and DBA/2J.
    Tissue: Brain.
  2. "Alternative-splicing in the exon-10 region of GABA(A) receptor beta(2) subunit gene: relationships between novel isoforms and psychotic disorders."
    Zhao C., Xu Z., Wang F., Chen J., Ng S.K., Wong P.W., Yu Z., Pun F.W., Ren L., Lo W.S., Tsang S.Y., Xue H.
    PLoS ONE 4:E6977-E6977(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-441, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. Cited for: INTERACTION WITH KCTD8; KCTD12; KCTD12B AND KCTD16, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGBRB2_MOUSE
AccessioniPrimary (citable) accession number: P63137
Secondary accession number(s): A6H6R7, D1LYT3, P15432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: March 23, 2010
Last modified: June 8, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.