Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endogenous retrovirus group K member 25 Pol protein

Gene

ERVK-25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution (By similarity).By similarity

Miscellaneous

This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries.
Exact N-terminus of this protein has not been formally described.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation
Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri585 – 626Integrase-typePROSITE-ProRule annotationAdd BLAST42
DNA bindingi809 – 857Integrase-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase
Biological processDNA integration, DNA recombination
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endogenous retrovirus group K member 25 Pol protein
Alternative name(s):
HERV-K_11q22.1 provirus ancestral Pol protein
Including the following 3 domains:
Reverse transcriptase (EC:2.7.7.49)
Short name:
RT
Ribonuclease H (EC:3.1.26.4)
Short name:
RNase H
Integrase
Short name:
IN
Gene namesi
Name:ERVK-25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:39039. ERVK-25.

Pathology & Biotechi

Organism-specific databases

DisGeNETi100862683.

Polymorphism and mutation databases

DMDMi52000774.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001867721 – 954Endogenous retrovirus group K member 25 Pol proteinAdd BLAST954

Proteomic databases

PeptideAtlasiP63136.
PRIDEiP63136.

Structurei

3D structure databases

ProteinModelPortaliP63136.
SMRiP63136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 245Reverse transcriptasePROSITE-ProRule annotationAdd BLAST189
Domaini461 – 588RNase HPROSITE-ProRule annotationAdd BLAST128
Domaini640 – 801Integrase catalyticPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi161 – 164LPQG4
Motifi195 – 198YXDD4

Domaini

The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri585 – 626Integrase-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG053630.
PhylomeDBiP63136.

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
3.30.420.10. 2 hits.
InterProiView protein in InterPro
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010661. RVT_thumb.
PfamiView protein in Pfam
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00078. RVT_1. 1 hit.
PF06817. RVT_thumb. 1 hit.
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiView protein in PROSITE
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P63136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NKSKKRRNRV SFLGAATVEP PKPIPLTWKT EKPVWVNQWP LPKQKLEALH
60 70 80 90 100
LLANEQLEKG HIEPSFSPWN SPVFVIQKKS GKWRMLTDLR AVNAVIQPMG
110 120 130 140 150
PLQPGLPSPA MIPKDWPLII IDLKDCFFTI PLAEQDCEKF AFTIPAINNK
160 170 180 190 200
EPATRFQWKV LPQGMLNSPT ICQTFVGRAL QPVREKFSDC YIIHYIDDIL
210 220 230 240 250
CAAETKDKLI DCYTFLQAEV ANAGLAIASD KIQTSTPFHY LGMQIENRKI
260 270 280 290 300
KPQKIEIRKD TLKALNDFQK LLGDINWIRP TLGIPTYAMS NLFSILRGDS
310 320 330 340 350
DLNSKRMLTP EATKEIKLVE EKIQSAQINR IDPLAPLQLL IFATAHSPTG
360 370 380 390 400
IIIQNTDLVE WSFLPHSTVK TFTLYLDQIA TLIGQTRLRI IKLCGNDPDK
410 420 430 440 450
IVVPLTKEQV RQAFINSGAW QIGLANFVGI IDNHYPKTKI FQFLKLTTWI
460 470 480 490 500
LPKITRREPL ENALTVFTDG SSNGKAAYTG PKERVIKTPY QSAQRAELVA
510 520 530 540 550
VITVLQDFDI NIISDSAYVV QATRDVETAL IKYSMDDQLN QLFNLLQQTV
560 570 580 590 600
RKRNFPFYIT HIRAHTNLPG PLTKANKQAD LLVSSALIKA QELHALTHVN
610 620 630 640 650
AAGLKNKFDV TWKLAKDIVQ HCTQCQVLHL PTQEAGVNPR GLCPNALWQM
660 670 680 690 700
DVTHVPSFGR LSYVHVTVDT YSHFIWATCH TGESTSHVKK HLLSCFAVMG
710 720 730 740 750
VPEKIKTDNG PGYCSKAFQK FLSQWKISHT TGIPYNSQGQ AIVERTNRTL
760 770 780 790 800
KTQLVKQKEG GDSKECTTPQ MQLNLALYTL NFLNIYRNQT TTSAEQHLTG
810 820 830 840 850
KKNSPHEGKL IWWKDNKNKT WEIGKVITWG RGFACVSPGE NQLPVWIPTR
860 870 880 890 900
HLKFYNEPIR DAKKSTSAET ETPQSSTVDS QDEQNGDVRR TDEVAIHQEG
910 920 930 940 950
RAANLGTTKE ADAVSYKISR EHKGDTNPRE YAACSLDDCI NGGKSPYACR

SSCS
Length:954
Mass (Da):107,472
Last modified:September 13, 2004 - v1
Checksum:i545C6BCD401CB4C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000776 Genomic DNA. No translation available.

Similar proteinsi

Entry informationi

Entry nameiPOK25_HUMAN
AccessioniPrimary (citable) accession number: P63136
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: August 30, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, ERV, Multifunctional enzyme, Reference proteome, Transposable element

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families