Reviewed, UniProtKB/Swiss-Prot P63134 (POK9_HUMAN)

Names and origin

Protein names	Recommended name:     HERV-K_5q13.3 provirus ancestral Pol protein Alternative name(s):     HERV-K104 Pol protein Including the following 1 domains:     1- Recommended name:             Reverse transcriptase                 Short name=RT               EC=2.7.7.49
Organism	Homo sapiens (Human) [Complete proteome]
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Domain	The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.
Miscellaneous	This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries. Exact N-terminus of this protein has not been formally described.
Sequence similarities	Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) subfamily. Contains 1 reverse transcriptase domain.
Caution	Truncated; premature stop codon after the reverse transcriptase domain.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Complete proteome ERV Transposable element
Gene Ontology (GO)
Biological process	RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	HERV-K_5q13.3 provirus ancestral Pol protein		PRO_0000186768
Regions
	Domain	57 – 245	189	Reverse transcriptase
	Motif	161 – 164	4	LPQG
	Motif	195 – 198	4	YXDD
Experimental info
	Sequence conflict	105	1	G → R Ref.1
	Sequence conflict	144	1	I → V Ref.1
	Sequence conflict	413	1	A → D Ref.1
	Sequence conflict	466	1	I → V Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P63134-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: A130A1D792E9EEC4	FASTA	483	54,772
	10 20 30 40 50 60 NKSKKRRNRV SFLGAATVEP PKPIPLTWKT EKPVWVNQWP LPKQKLEALH LLANEQLEKG 70 80 90 100 110 120 HIEPSFSPWN SPVFVIQKKS GKWRMLTDLR AVNAIIQPMG PLQSGLPSPA MIPKDWPLII 130 140 150 160 170 180 IDLKDCFFTI PLAEQDCEKF AFTILAINNK EPATRFQWKV LPQGMLNSPT ICQTFVGRAL 190 200 210 220 230 240 QPVRDKFSDC YIIHYIDDIL CAAETKDKLI DCYTFLQAEV ANAGLAIASD KIQTSTPFHY 250 260 270 280 290 300 LGMQIENRKI KPQKIEIRKY TLKTLNDFQK LLGDINWIRP TLGIPTYAMS NLFSILRGDS 310 320 330 340 350 360 DLNSKRMLTP EATKEIKLVE EKIQSVQINR IDPLAPLQLL IFATAHSPTG IIIQNTDLVE 370 380 390 400 410 420 WSFLPHSTVK TFTLYLDQIA TLIGQTRLRI IKLCGNDPDK IVVPLTKEQV RQAFINSGAW 430 440 450 460 470 480 QIGLANFVGI IDNHYPKTKI FQFLKLTTWI LPKITRHEPL ENALTIFTDG SSNGKAAYTG PKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Many human endogenous retrovirus K (HERV-K) proviruses are unique to humans." Barbulescu M., Turner G., Seaman M.I., Deinard A.S., Kidd K.K., Lenz J. Curr. Biol. 9:861-868(1999) [PubMed: 10469592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Web resources

Human Endogenous Retrovirus Database (HERVd)

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF164612 Genomic DNA. No translation available. AC025757 Genomic DNA. No translation available.
IPI	IPI00742960.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P63134.
Enzyme and pathway databases
BRENDA	2.7.7.49. 247.
Gene expression databases
Genevestigator	P63134.
Family and domain databases
InterPro	IPR000477. Reverse_transcriptase. IPR010661. RVT_thumb. [Graphical view]
Pfam	PF00078. RVT_1. 1 hit. PF06817. RVT_thumb. 1 hit. [Graphical view]
PROSITE	PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POK9_HUMAN

Accession

Primary (citable) accession number: P63134

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.