Reviewed, UniProtKB/Swiss-Prot P63133 (POK6_HUMAN)

Names and origin

Protein names	Recommended name:     HERV-K_8p23.1 provirus ancestral Pol protein Alternative name(s):     HERV-K115 Pol protein Including the following 3 domains:     1- Recommended name:             Reverse transcriptase                 Short name=RT               EC=2.7.7.49     2- Recommended name:             Ribonuclease H                 Short name=RNase H               EC=3.1.26.4     3- Recommended name:             Integrase                 Short name=IN
Organism	Homo sapiens (Human) [Complete proteome]
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Endonucleolytic cleavage to 5'-phosphomonoester.
Domain	The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.
Miscellaneous	This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries. Exact N-terminus of this protein has not been formally described. Insertional polymorphism. Provirus present in 16% of tested individuals. Intragenic, in first intron of DEFB107 gene.
Sequence similarities	Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) subfamily. Contains 1 integrase catalytic domain. Contains 1 integrase-type DNA-binding domain. Contains 1 integrase-type zinc finger. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Complete proteome ERV Multifunctional enzyme Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW integrase activity Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 956	956	HERV-K_8p23.1 provirus ancestral Pol protein		PRO_0000186766
Regions
	Domain	57 – 245	189	Reverse transcriptase
	Domain	461 – 590	130	RNase H
	Domain	642 – 803	162	Integrase catalytic
	Zinc finger	587 – 628	42	Integrase-type
	DNA binding	811 – 859	49	Integrase-type
	Motif	161 – 164	4	LPQG
	Motif	195 – 198	4	YXDD

Sequences

	Sequence		Length	Mass (Da)
	P63133-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 2A267FE1EF31F06B	FASTA	956	107,703
	10 20 30 40 50 60 NKSKKRRNRV SFLGVATIEP PKPIPLTWKT EKLVWVNQWP LPKQKLEALH LLANEQLEKG 70 80 90 100 110 120 HIEPSFSPWN SPVFVIQKKS GKWRMLTDLR AVNAVIQPMG PLQPGLPSPA MIPKDWPLII 130 140 150 160 170 180 IDLKDCFFTI PLAEQDCEKF AFTIPAINNK EPATRFQWKV LPQGMLNSPT ICQTFVGRAL 190 200 210 220 230 240 QPVRKKFSDC YIIHYIDDIL CAAETKDKLI DCYTFLQAEV ASAGLAIASD KIQTSTPFHY 250 260 270 280 290 300 LGMQIENRKI KPQKIEIRKD TLKTLNDFQK LLGDINWIQP TLGIPTYAMS NLFSILRGDS 310 320 330 340 350 360 DLNSKRILTP EATKEIKLVE EKIQSAQINR IDPLAPLQLL IFATAHSPTG IIIQNTDLVE 370 380 390 400 410 420 WSFLPHSTVK TFTLYLDQIA TLIGQTRLRI IKLCGNDPDK IVVPLTKEQV RQAFINSGAW 430 440 450 460 470 480 QIGLANFVGI IDNHYPKTKI FQFLKLTTWI LPKITRREPL ENALTVFTDG SSNGKAAYTG 490 500 510 520 530 540 PKERVIKTPY QSAQRAELVA VITVLQDFDQ PINIISDSAY VVQATRVVET ALIKYSMDDQ 550 560 570 580 590 600 LNQLFNLLQQ TVRKRNFPFY ITHIRAHTNL PGPLTKANEQ ADLLVSSALI KAQELHALTH 610 620 630 640 650 660 VNAAGLKNKF DVTWKQAKDI VQHCTQCQVL HLPTQEAGVN PRGLCPNALW QMDVTHVPSF 670 680 690 700 710 720 GRLSYVHVTV DTYSHFIWAT CQTGESTSHV KKHLLSCFAV MGVPEKIKTD NGPGYCSKAF 730 740 750 760 770 780 QKFLSQWKIS HTTGIPYNSQ GQAIVERTNR TLKTQLVKQK EGGDSKECTT PQMQLNLALY 790 800 810 820 830 840 TLNFLNIYRN QTTTSAEQHL TGKKNSPHEG KLIWWKDNKN KTWEIGKVIT WGRGFACVSP 850 860 870 880 890 900 GENQLPVWIP TRHLKFYNEP IRDAKKSTSA ETETPQSSTV DSQDEQNGDV RRTDEVAIHQ 910 920 930 940 950 EGRAADLGTT KEADAVSYKI SREHKGDTNP REYAACSLDD CINGGKSPYA CRSSCS

References

[1]	"Insertional polymorphisms of full-length endogenous retroviruses in humans." Turner G., Barbulescu M., Su M., Jensen-Seaman M.I., Kidd K.K., Lenz J. Curr. Biol. 11:1531-1535(2001) [PubMed: 11591322] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY037929 Genomic DNA. No translation available.
IPI	IPI00454617.
3D structure databases
SMR	P63133. Positions 23-589, 589-629, 643-785.
ModBase	Search...
Proteomic databases
PRIDE	P63133.
Phylogenomic databases
HOVERGEN	P63133.
Enzyme and pathway databases
BRENDA	2.7.7.49. 247. 3.1.26.4. 247.
Gene expression databases
Genevestigator	P63133.
Family and domain databases
InterPro	IPR001037. Integrase_C_retrovir. IPR001584. Integrase_cat-core. IPR003308. Integrase_Zn-bd_dom_N. IPR012337. PolynucTfrase_RNaseH_fold. IPR000477. Reverse_transcriptase. IPR002156. RNase_H. IPR010661. RVT_thumb. [Graphical view]
Pfam	PF00552. Integrase. 1 hit. PF02022. Integrase_Zn. 1 hit. PF00075. RnaseH. 1 hit. PF00665. rve. 1 hit. PF00078. RVT_1. 1 hit. PF06817. RVT_thumb. 1 hit. [Graphical view]
PROSITE	PS50994. INTEGRASE. 1 hit. PS51027. INTEGRASE_DBD. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. PS50876. ZF_INTEGRASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POK6_HUMAN

Accession

Primary (citable) accession number: P63133

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.