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Protein

Endogenous retrovirus group K member 9 Pol protein

Gene

ERVK-9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The products of the Gag polyproteins of infectious retroviruses perform highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Endogenous Gag proteins may have kept, lost or modified their original function during evolution (By similarity).By similarity
Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution (By similarity).By similarity

Caution

Truncated; frameshift leads to premature stop codon.Curated

Catalytic activityi

Processing at the authentic HIV-1 PR recognition site and release of the mature p17 matrix and the p24 capsid protein, as a result of the cleavage of the -SQNY-|-PIVQ- cleavage site.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation
Endonucleolytic cleavage to 5'-phosphomonoester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei805PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri544 – 561CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri580 – 597CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Protease, RNA-binding, RNA-directed DNA polymerase, Transferase
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endogenous retrovirus group K member 9 Pol protein
Alternative name(s):
HERV-K(C6) Gag-Pol protein
HERV-K109 Gag-Pol protein
HERV-K_6q14.1 provirus ancestral Gag-Pol polyprotein
Including the following 2 domains:
Protease (EC:3.4.23.50)
Alternative name(s):
PR
Retropepsin
Reverse transcriptase/ribonuclease H (EC:2.7.7.49, EC:2.7.7.7, EC:3.1.26.4)
Alternative name(s):
p66 RT
Gene namesi
Name:ERVK-9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:39005 ERVK-9
neXtProtiNX_P63128

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi100616102

Polymorphism and mutation databases

DMDMi118572691

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00001867642 – 1117Endogenous retrovirus group K member 9 Pol proteinAdd BLAST1116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

Myristoylation is essential for retroviral assembly. Alteration of the glycine residue leads to a block in the budding of particles and an accumulation of Gag inside the cell (By similarity).By similarity
Specific enzymatic cleavages may yield mature proteins.Curated

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP63128
PRIDEiP63128

PTM databases

iPTMnetiP63128
PhosphoSitePlusiP63128

Structurei

3D structure databases

ProteinModelPortaliP63128
SMRiP63128
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 195Reverse transcriptasePROSITE-ProRule annotationAdd BLAST138
Domaini800 – 875Peptidase A2PROSITE-ProRule annotationAdd BLAST76
Domaini890 – 936G-patchPROSITE-ProRule annotationAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi168 – 257Pro-richAdd BLAST90
Compositional biasi611 – 666Gln-richAdd BLAST56

Domaini

The LPQG motifs are catalytically important and conserved among many retroviruses.
HERV-K Gag polyprotein contains regions homologous to the matrix (MA), capsid (CA) and nucleocapsid (NC) proteins from infectious retroviruses. Evidence suggests that HERV-K(HML-2) Gag polyprotein can be cleaved into mature MA, CA and NC under certain circumstances. However, the exact boundaries as well as the size of processed Gag proteins have not been precisely determined yet.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri544 – 561CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri580 – 597CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG082155
PhylomeDBiP63128

Family and domain databases

CDDicd05482 HIV_retropepsin_like, 1 hit
cd07557 trimeric_dUTPase, 1 hit
Gene3Di1.10.1200.301 hit
1.10.150.4901 hit
1.10.375.101 hit
2.40.70.101 hit
2.70.40.101 hit
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR003322 B_retro_matrix
IPR038124 B_retro_matrix_sf
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR033704 dUTPase_trimeric
IPR000467 G_patch_dom
IPR000721 Gag_p24
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR034170 Retropepsin-like_cat_dom
IPR018061 Retropepsins
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR000477 RT_dom
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
PF01585 G-patch, 1 hit
PF02337 Gag_p10, 1 hit
PF00607 Gag_p24, 1 hit
PF00077 RVP, 1 hit
PF00078 RVT_1, 1 hit
PF00098 zf-CCHC, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265 B_retro_matrix_N, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF50630 SSF50630, 1 hit
SSF51283 SSF51283, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50174 G_PATCH, 1 hit
PS50878 RT_POL, 1 hit
PS50158 ZF_CCHC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Note: This protein is synthesized as a Gag polypeptide and as a Gag-Pro-Pol polyprotein. The later is the precursor of the Pro and Pol proteins. It is thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries.
Isoform 1 (identifier: P63128-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTKSKIKS KYASYLSFIK ILLKRGGVKV STKNLIKLFQ IIEQFCPWFP
60 70 80 90 100
EQGTLDLKDW KRIGKELKQA GRKGNIIPLT VWNDWAIIKA ALEPFQTEED
110 120 130 140 150
SISVSDAPGS GIIDCNEKTR KKSQKETESL HCEYVAEPVM AQSTQNVDYN
160 170 180 190 200
QLQEVIYPET LKLEGKGPEL VGPSESKPRG TSPLPAGQVP VTLQPQKQVK
210 220 230 240 250
ENKTQPPVAY QYWPPAELQY RPPPESQYGY PGMPPAPQGR APYPQPPTRR
260 270 280 290 300
LNPTAPPSRQ GSELHEIIDK SRKEGDTEAW QFPVTLEPMP PGEGAQEGEP
310 320 330 340 350
PTVEARYKSF SIKILKDMKE GVKQYGPNSP YMRTLLDSIA HGHRLIPYDW
360 370 380 390 400
EILAKSSLSP SQFLQFKTWW IDGVQEQVRR NRAANPPVNI DADQLLGIGQ
410 420 430 440 450
NWSTISQQAL MQNEAIEQVR AICLRAWEKI QDPGSTCPSF NTVRQGSKEP
460 470 480 490 500
YPDFVARLQD VAQKSIADEK ARKVIVELMA YENANPECQS AIKPLKGKVP
510 520 530 540 550
AGSDVISEYV KACDGIGGAM HKAMLMAQAI TGVVLGGQVR TFGGKCYNCG
560 570 580 590 600
QIGHLKKNCP VLNKQNITIQ ATTTGREPPD LCPRCKKGKH WASQCRSKFD
610 620 630 640 650
KNGQPLSGNE QRGQPQAPQQ TGAFPIQPFV PQGFQGQQPP LSQVFQGISQ
660 670 680 690 700
LPQYNNCPPP QVAVQQVDLC TIQAVSLLPG EPPQKIPTGV YGPLPEGTVG
710 720 730 740 750
LILGRSSLNL KGVQIHTSVV DSDYKGEIQL VISSSVPWSA SPGDRIAQLL
760 770 780 790 800
LLPYIKGGNS EIKRIGGLGS TDPTGKAAYW ASQVSENRPV CKAIIQGKQF
810 820 830 840 850
EGLVDTGADV SIIALNQWPK NWPKQKAVTG LVGIGTASEV YQSMEILHCL
860 870 880 890 900
GPDNQESTVQ PMITSIPLNL WGRDLLQQWG AEITMPAPLY SPTSQKIMTK
910 920 930 940 950
RGYIPGKGLG KNEDGIKIPF EAKINQKREG IGYPFLGAAT IEPPKPIPLT
960 970 980 990 1000
WKTEKPVWVN QWPLPKQKLE ALHLLANEQL EKGHIEPSFS PWNSPVFVIQ
1010 1020 1030 1040 1050
KKSGKWRMLT DLRAVNAVIQ PMGPLQPGLP SPAMIPKDWP LIIIDLKDCF
1060 1070 1080 1090 1100
FTIPLAEQDC EKFAFTIPAI NNKEPATRFQ WKVLPQGMLN SPTICQTFVG
1110
RALQPVKVFR LLYYSLY
Length:1,117
Mass (Da):123,620
Last modified:January 23, 2007 - v3
Checksum:iF7614F482B4117DD
GO
Isoform 2 (identifier: P63126-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P63126.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Gag-Pro polyprotein is produced from conventional translation of the gag ORF.
Length:666
Mass (Da):74,005
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164615 Genomic DNA Translation: AAD51799.1 Sequence problems.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiPOK9_HUMAN
AccessioniPrimary (citable) accession number: P63128
Secondary accession number(s): Q9UKH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 101 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, ERV, Reference proteome, Transposable element