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P63102

- 1433Z_RAT

UniProt

P63102 - 1433Z_RAT

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Protein
14-3-3 protein zeta/delta
Gene
Ywhaz, Msfs1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Interaction with phosphoserine on interacting protein By similarity
Sitei127 – 1271Interaction with phosphoserine on interacting protein By similarity

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. protein complex binding Source: RGD
  3. protein domain specific binding Source: UniProtKB

GO - Biological processi

  1. histamine secretion by mast cell Source: RGD
  2. protein targeting to mitochondrion Source: RGD
  3. regulation of cell death Source: Ensembl
  4. response to drug Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_199176. Translocation of GLUT4 to the plasma membrane.
REACT_202871. KSRP destabilizes mRNA.
REACT_214025. deactivation of the beta-catenin transactivating complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein zeta/delta
Alternative name(s):
Mitochondrial import stimulation factor S1 subunit
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
Gene namesi
Name:Ywhaz
Synonyms:Msfs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi3980. Ywhaz.

Subcellular locationi

Cytoplasm. Melanosome
Note: Located to stage I to stage IV melanosomes By similarity.

GO - Cellular componenti

  1. cell leading edge Source: RGD
  2. extracellular vesicular exosome Source: Ensembl
  3. mast cell granule Source: GOC
  4. melanosome Source: UniProtKB-SubCell
  5. mitochondrion Source: RGD
  6. nucleus Source: Ensembl
  7. perinuclear region of cytoplasm Source: RGD
  8. postsynaptic density Source: RGD
  9. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24524514-3-3 protein zeta/delta
PRO_0000058630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei3 – 31N6-acetyllysine By similarity
Modified residuei58 – 581Phosphoserine; by PKA By similarity
Modified residuei68 – 681N6-acetyllysine By similarity
Modified residuei184 – 1841Phosphoserine By similarity
Modified residuei207 – 2071Phosphoserine By similarity
Modified residuei232 – 2321Phosphothreonine; by CK1 By similarity

Post-translational modificationi

The delta, brain-specific form differs from the zeta form in being phosphorylated By similarity. Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1 By similarity. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53 By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP63102.
PRIDEiP63102.

PTM databases

PhosphoSiteiP63102.

Expressioni

Tissue specificityi

Highly expressed in brain (at protein level).1 Publication

Gene expression databases

GenevestigatoriP63102.

Interactioni

Subunit structurei

Homodimer. Heterodimerizes with YWHAE By similarity. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 By similarity. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with CDK16 and with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization By similarity. Interacts with GAB2 and SAMSN1. Binds to TLK2 By similarity. Interacts with BSPRY. Interacts with BCL2L11.3 Publications

Protein-protein interaction databases

BioGridi247609. 5 interactions.
DIPiDIP-36916N.
DIP-488N.
IntActiP63102. 5 interactions.
MINTiMINT-197638.

Structurei

3D structure databases

ProteinModelPortaliP63102.
SMRiP63102. Positions 1-230.

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00730000110424.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP63102.
KOiK16197.
OMAiRYDDMAG.
OrthoDBiEOG7HHWT3.
PhylomeDBiP63102.
TreeFamiTF102003.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63102-1 [UniParc]FASTAAdd to Basket

« Hide

MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN    50
VVGARRSSWR VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL 100
LEKFLIPNAS QPESKVFYLK MKGDYYRYLA EVAAGDDKKG IVDQSQQAYQ 150
EAFEISKKEM QPTHPIRLGL ALNFSVFYYE ILNSPEKACS LAKTAFDEAI 200
AELDTLSEES YKDSTLIMQL LRDNLTLWTS DTQGDEAEAG EGGEN 245
Length:245
Mass (Da):27,771
Last modified:September 13, 2004 - v1
Checksum:i2164DF3793B45B7A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881T → M in AAA80544. 1 Publication
Sequence conflicti109 – 1091A → R in AAC37660. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17615 mRNA. Translation: BAA04534.1.
U37252 mRNA. Translation: AAA80544.1.
D30740 mRNA. Translation: BAA06402.1.
BC070941 mRNA. Translation: AAH70941.2.
BC094305 mRNA. Translation: AAH94305.1.
L07913 mRNA. Translation: AAC37660.1.
PIRiJC2502.
JC5232.
S59915.
RefSeqiNP_037143.2. NM_013011.3.
XP_006241591.1. XM_006241529.1.
UniGeneiRn.1292.

Genome annotation databases

EnsembliENSRNOT00000035628; ENSRNOP00000030885; ENSRNOG00000008195.
GeneIDi25578.
KEGGirno:25578.
UCSCiRGD:3980. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17615 mRNA. Translation: BAA04534.1 .
U37252 mRNA. Translation: AAA80544.1 .
D30740 mRNA. Translation: BAA06402.1 .
BC070941 mRNA. Translation: AAH70941.2 .
BC094305 mRNA. Translation: AAH94305.1 .
L07913 mRNA. Translation: AAC37660.1 .
PIRi JC2502.
JC5232.
S59915.
RefSeqi NP_037143.2. NM_013011.3.
XP_006241591.1. XM_006241529.1.
UniGenei Rn.1292.

3D structure databases

ProteinModelPortali P63102.
SMRi P63102. Positions 1-230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247609. 5 interactions.
DIPi DIP-36916N.
DIP-488N.
IntActi P63102. 5 interactions.
MINTi MINT-197638.

PTM databases

PhosphoSitei P63102.

Proteomic databases

PaxDbi P63102.
PRIDEi P63102.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000035628 ; ENSRNOP00000030885 ; ENSRNOG00000008195 .
GeneIDi 25578.
KEGGi rno:25578.
UCSCi RGD:3980. rat.

Organism-specific databases

CTDi 7534.
RGDi 3980. Ywhaz.

Phylogenomic databases

eggNOGi COG5040.
GeneTreei ENSGT00730000110424.
HOGENOMi HOG000240379.
HOVERGENi HBG050423.
InParanoidi P63102.
KOi K16197.
OMAi RYDDMAG.
OrthoDBi EOG7HHWT3.
PhylomeDBi P63102.
TreeFami TF102003.

Enzyme and pathway databases

Reactomei REACT_199176. Translocation of GLUT4 to the plasma membrane.
REACT_202871. KSRP destabilizes mRNA.
REACT_214025. deactivation of the beta-catenin transactivating complex.

Miscellaneous databases

NextBioi 303003.
PROi P63102.

Gene expression databases

Genevestigatori P63102.

Family and domain databases

Gene3Di 1.20.190.20. 1 hit.
InterProi IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view ]
PANTHERi PTHR18860. PTHR18860. 1 hit.
Pfami PF00244. 14-3-3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000868. 14-3-3. 1 hit.
PRINTSi PR00305. 1433ZETA.
SMARTi SM00101. 14_3_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48445. SSF48445. 1 hit.
PROSITEi PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of rat cDNAs for the zeta and theta subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain."
    Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H., Inoue Y.
    Brain Res. Mol. Brain Res. 25:113-121(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "A gene variation of 14-3-3 zeta isoform in rat hippocampus."
    Murakami K., Situ S.Y., Eshete F.
    Gene 179:245-249(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  3. "cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol."
    Alam R., Hachiya N., Sakaguchi M., Shun-Ichiro K., Iwanaga S., Kitajima M., Mihara K., Omura T.
    J. Biochem. 116:416-425(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Lung.
  5. Bienvenut W.V., von Kriegsheim A., Kolch W.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-9; 12-49; 61-68; 92-115; 128-157 AND 213-222, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fibroblast and Pheochromocytoma.
  6. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 12-55; 57-68; 84-115; 121-167; 159-187 AND 194-245, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  7. "Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis."
    Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C.
    Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-41; 61-68 AND 128-139, INTERACTION WITH AANAT, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Cloning and characterization of the epsilon and zeta isoforms of the 14-3-3 proteins."
    Roseboom P.H., Weller J.L., Babila T., Aitken A., Sellers L.A., Moffet J.R., Namboodiri M.A., Klein D.C.
    DNA Cell Biol. 13:629-640(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-245.
    Strain: Sprague-Dawley.
    Tissue: Pineal gland.
  9. "Characterization of zetin 1/rBSPRY, a novel binding partner of 14-3-3 proteins."
    Birkenfeld J., Kartmann B., Anliker B., Ono K., Schloetcke B., Betz H., Roth D.
    Biochem. Biophys. Res. Commun. 302:526-533(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH BSPRY.
  10. "Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection."
    Thompson S., Pearson A.N., Ashley M.D., Jessick V., Murphy B.M., Gafken P., Henshall D.C., Morris K.T., Simon R.P., Meller R.
    J. Biol. Chem. 286:19331-19339(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BCL2L11.

Entry informationi

Entry namei1433Z_RAT
AccessioniPrimary (citable) accession number: P63102
Secondary accession number(s): P35215
, P70197, P97286, Q52KK1, Q6IRF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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