Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P63102

- 1433Z_RAT

UniProt

P63102 - 1433Z_RAT

Protein

14-3-3 protein zeta/delta

Gene

Ywhaz

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei56 – 561Interaction with phosphoserine on interacting proteinBy similarity
    Sitei127 – 1271Interaction with phosphoserine on interacting proteinBy similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein complex binding Source: RGD
    3. protein domain specific binding Source: UniProtKB

    GO - Biological processi

    1. histamine secretion by mast cell Source: RGD
    2. protein targeting to mitochondrion Source: RGD
    3. regulation of cell death Source: Ensembl
    4. response to drug Source: RGD

    Enzyme and pathway databases

    ReactomeiREACT_199176. Translocation of GLUT4 to the plasma membrane.
    REACT_202871. KSRP destabilizes mRNA.
    REACT_214025. deactivation of the beta-catenin transactivating complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    14-3-3 protein zeta/delta
    Alternative name(s):
    Mitochondrial import stimulation factor S1 subunit
    Protein kinase C inhibitor protein 1
    Short name:
    KCIP-1
    Gene namesi
    Name:Ywhaz
    Synonyms:Msfs1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi3980. Ywhaz.

    Subcellular locationi

    Cytoplasm. Melanosome
    Note: Located to stage I to stage IV melanosomes.By similarity

    GO - Cellular componenti

    1. cell leading edge Source: RGD
    2. extracellular vesicular exosome Source: Ensembl
    3. mast cell granule Source: GOC
    4. melanosome Source: UniProtKB-SubCell
    5. mitochondrion Source: RGD
    6. nucleus Source: Ensembl
    7. perinuclear region of cytoplasm Source: RGD
    8. postsynaptic density Source: RGD
    9. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 24524514-3-3 protein zeta/deltaPRO_0000058630Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei3 – 31N6-acetyllysineBy similarity
    Modified residuei58 – 581Phosphoserine; by PKABy similarity
    Modified residuei68 – 681N6-acetyllysineBy similarity
    Modified residuei184 – 1841PhosphoserineBy similarity
    Modified residuei207 – 2071PhosphoserineBy similarity
    Modified residuei232 – 2321Phosphothreonine; by CK1By similarity

    Post-translational modificationi

    The delta, brain-specific form differs from the zeta form in being phosphorylated By similarity. Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1 By similarity. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP63102.
    PRIDEiP63102.

    PTM databases

    PhosphoSiteiP63102.

    Expressioni

    Tissue specificityi

    Highly expressed in brain (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriP63102.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimerizes with YWHAE By similarity. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 By similarity. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with CDK16 and with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization By similarity. Interacts with GAB2 and SAMSN1. Binds to TLK2 By similarity. Interacts with BSPRY. Interacts with BCL2L11.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi247609. 5 interactions.
    DIPiDIP-36916N.
    DIP-488N.
    IntActiP63102. 6 interactions.
    MINTiMINT-197638.

    Structurei

    3D structure databases

    ProteinModelPortaliP63102.
    SMRiP63102. Positions 1-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 14-3-3 family.Curated

    Phylogenomic databases

    eggNOGiCOG5040.
    GeneTreeiENSGT00730000110424.
    HOGENOMiHOG000240379.
    HOVERGENiHBG050423.
    InParanoidiP63102.
    KOiK16197.
    OMAiRYDDMAG.
    OrthoDBiEOG7HHWT3.
    PhylomeDBiP63102.
    TreeFamiTF102003.

    Family and domain databases

    Gene3Di1.20.190.20. 1 hit.
    InterProiIPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view]
    PANTHERiPTHR18860. PTHR18860. 1 hit.
    PfamiPF00244. 14-3-3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000868. 14-3-3. 1 hit.
    PRINTSiPR00305. 1433ZETA.
    SMARTiSM00101. 14_3_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48445. SSF48445. 1 hit.
    PROSITEiPS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63102-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN    50
    VVGARRSSWR VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL 100
    LEKFLIPNAS QPESKVFYLK MKGDYYRYLA EVAAGDDKKG IVDQSQQAYQ 150
    EAFEISKKEM QPTHPIRLGL ALNFSVFYYE ILNSPEKACS LAKTAFDEAI 200
    AELDTLSEES YKDSTLIMQL LRDNLTLWTS DTQGDEAEAG EGGEN 245
    Length:245
    Mass (Da):27,771
    Last modified:September 13, 2004 - v1
    Checksum:i2164DF3793B45B7A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 881T → M in AAA80544. (PubMed:8972907)Curated
    Sequence conflicti109 – 1091A → R in AAC37660. (PubMed:8024705)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17615 mRNA. Translation: BAA04534.1.
    U37252 mRNA. Translation: AAA80544.1.
    D30740 mRNA. Translation: BAA06402.1.
    BC070941 mRNA. Translation: AAH70941.2.
    BC094305 mRNA. Translation: AAH94305.1.
    L07913 mRNA. Translation: AAC37660.1.
    PIRiJC2502.
    JC5232.
    S59915.
    RefSeqiNP_037143.2. NM_013011.3.
    XP_006241591.1. XM_006241529.1.
    UniGeneiRn.1292.

    Genome annotation databases

    EnsembliENSRNOT00000035628; ENSRNOP00000030885; ENSRNOG00000008195.
    GeneIDi25578.
    KEGGirno:25578.
    UCSCiRGD:3980. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17615 mRNA. Translation: BAA04534.1 .
    U37252 mRNA. Translation: AAA80544.1 .
    D30740 mRNA. Translation: BAA06402.1 .
    BC070941 mRNA. Translation: AAH70941.2 .
    BC094305 mRNA. Translation: AAH94305.1 .
    L07913 mRNA. Translation: AAC37660.1 .
    PIRi JC2502.
    JC5232.
    S59915.
    RefSeqi NP_037143.2. NM_013011.3.
    XP_006241591.1. XM_006241529.1.
    UniGenei Rn.1292.

    3D structure databases

    ProteinModelPortali P63102.
    SMRi P63102. Positions 1-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247609. 5 interactions.
    DIPi DIP-36916N.
    DIP-488N.
    IntActi P63102. 6 interactions.
    MINTi MINT-197638.

    PTM databases

    PhosphoSitei P63102.

    Proteomic databases

    PaxDbi P63102.
    PRIDEi P63102.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000035628 ; ENSRNOP00000030885 ; ENSRNOG00000008195 .
    GeneIDi 25578.
    KEGGi rno:25578.
    UCSCi RGD:3980. rat.

    Organism-specific databases

    CTDi 7534.
    RGDi 3980. Ywhaz.

    Phylogenomic databases

    eggNOGi COG5040.
    GeneTreei ENSGT00730000110424.
    HOGENOMi HOG000240379.
    HOVERGENi HBG050423.
    InParanoidi P63102.
    KOi K16197.
    OMAi RYDDMAG.
    OrthoDBi EOG7HHWT3.
    PhylomeDBi P63102.
    TreeFami TF102003.

    Enzyme and pathway databases

    Reactomei REACT_199176. Translocation of GLUT4 to the plasma membrane.
    REACT_202871. KSRP destabilizes mRNA.
    REACT_214025. deactivation of the beta-catenin transactivating complex.

    Miscellaneous databases

    NextBioi 303003.
    PROi P63102.

    Gene expression databases

    Genevestigatori P63102.

    Family and domain databases

    Gene3Di 1.20.190.20. 1 hit.
    InterProi IPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view ]
    PANTHERi PTHR18860. PTHR18860. 1 hit.
    Pfami PF00244. 14-3-3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000868. 14-3-3. 1 hit.
    PRINTSi PR00305. 1433ZETA.
    SMARTi SM00101. 14_3_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48445. SSF48445. 1 hit.
    PROSITEi PS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of rat cDNAs for the zeta and theta subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain."
      Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H., Inoue Y.
      Brain Res. Mol. Brain Res. 25:113-121(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "A gene variation of 14-3-3 zeta isoform in rat hippocampus."
      Murakami K., Situ S.Y., Eshete F.
      Gene 179:245-249(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    3. "cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol."
      Alam R., Hachiya N., Sakaguchi M., Shun-Ichiro K., Iwanaga S., Kitajima M., Mihara K., Omura T.
      J. Biochem. 116:416-425(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Lung.
    5. Bienvenut W.V., von Kriegsheim A., Kolch W.
      Submitted (JUN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-9; 12-49; 61-68; 92-115; 128-157 AND 213-222, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Fibroblast and Pheochromocytoma.
    6. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 12-55; 57-68; 84-115; 121-167; 159-187 AND 194-245, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    7. "Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis."
      Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C.
      Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-41; 61-68 AND 128-139, INTERACTION WITH AANAT, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Cloning and characterization of the epsilon and zeta isoforms of the 14-3-3 proteins."
      Roseboom P.H., Weller J.L., Babila T., Aitken A., Sellers L.A., Moffet J.R., Namboodiri M.A., Klein D.C.
      DNA Cell Biol. 13:629-640(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-245.
      Strain: Sprague-Dawley.
      Tissue: Pineal gland.
    9. "Characterization of zetin 1/rBSPRY, a novel binding partner of 14-3-3 proteins."
      Birkenfeld J., Kartmann B., Anliker B., Ono K., Schloetcke B., Betz H., Roth D.
      Biochem. Biophys. Res. Commun. 302:526-533(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INTERACTION WITH BSPRY.
    10. "Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection."
      Thompson S., Pearson A.N., Ashley M.D., Jessick V., Murphy B.M., Gafken P., Henshall D.C., Morris K.T., Simon R.P., Meller R.
      J. Biol. Chem. 286:19331-19339(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BCL2L11.

    Entry informationi

    Entry namei1433Z_RAT
    AccessioniPrimary (citable) accession number: P63102
    Secondary accession number(s): P35215
    , P70197, P97286, Q52KK1, Q6IRF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3