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Protein

14-3-3 protein zeta/delta

Gene

Ywhaz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

GO - Molecular functioni

GO - Biological processi

  • establishment of Golgi localization Source: MGI
  • Golgi reassembly Source: MGI
  • protein targeting Source: MGI
  • regulation of cell death Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-392517. Rap1 signalling.
R-MMU-430116. GP1b-IX-V activation signalling.
R-MMU-450604. KSRP (KHSRP) binds and destabilizes mRNA.
R-MMU-512988. Interleukin-3, 5 and GM-CSF signaling.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein zeta/delta
Alternative name(s):
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
SEZ-2
Gene namesi
Name:Ywhaz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109484. Ywhaz.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586281 – 24514-3-3 protein zeta/deltaAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei58Phosphoserine; by PKA1 Publication1
Modified residuei68N6-acetyllysineBy similarity1
Modified residuei184PhosphoserineBy similarity1
Modified residuei207PhosphoserineCombined sources1
Modified residuei210PhosphoserineBy similarity1
Modified residuei232Phosphothreonine; by CK1By similarity1

Post-translational modificationi

The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1 (By similarity). Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63101.
MaxQBiP63101.
PaxDbiP63101.
PeptideAtlasiP63101.
PRIDEiP63101.
TopDownProteomicsiP63101.

2D gel databases

REPRODUCTION-2DPAGEiP63101.
UCD-2DPAGEiP63101.

PTM databases

iPTMnetiP63101.
PhosphoSitePlusiP63101.
SwissPalmiP63101.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022285.
ExpressionAtlasiP63101. baseline and differential.
GenevisibleiP63101. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimerizes with YWHAE (By similarity). Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 (By similarity). Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with CDK16 and with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with BSPRY. Interacts with Thr-phosphorylated ITGB2 (By similarity). Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation (By similarity). It may also prevent thiol-dependent inactivation (By similarity). Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization (By similarity). Interacts with GAB2 (By similarity). Interacts with SAMSN1. Interacts with BCL2L11 and TLK2. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways (PubMed:22701344). Interacts with SLITRK1 (By similarity).By similarity8 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting proteinBy similarity1
Sitei127Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204623. 168 interactors.
CORUMiP63101.
DIPiDIP-31894N.
IntActiP63101. 695 interactors.
MINTiMINT-101845.
STRINGi10090.ENSMUSP00000022894.

Structurei

3D structure databases

ProteinModelPortaliP63101.
SMRiP63101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP63101.
KOiK16197.
OMAiLWTSSDS.
OrthoDBiEOG091G0VKY.
PhylomeDBiP63101.
TreeFamiTF102003.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P63101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGARRSSWR VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL
110 120 130 140 150
LEKFLIPNAS QPESKVFYLK MKGDYYRYLA EVAAGDDKKG IVDQSQQAYQ
160 170 180 190 200
EAFEISKKEM QPTHPIRLGL ALNFSVFYYE ILNSPEKACS LAKTAFDEAI
210 220 230 240
AELDTLSEES YKDSTLIMQL LRDNLTLWTS DTQGDEAEAG EGGEN
Length:245
Mass (Da):27,771
Last modified:September 13, 2004 - v1
Checksum:i2164DF3793B45B7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78M → V in BAA11751 (PubMed:9016762).Curated1
Sequence conflicti139K → R in BAE36724 (PubMed:16141072).Curated1
Sequence conflicti218 – 219MQ → IE in BAA13421 (Ref. 2) Curated2
Sequence conflicti236E → D in BAA11751 (PubMed:9016762).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78647 mRNA. Translation: BAA11464.1.
D87660 mRNA. Translation: BAA13421.1.
U79231 mRNA. Translation: AAC53254.1.
D83037 mRNA. Translation: BAA11751.1.
AK083368 mRNA. Translation: BAC38887.1.
AK145657 mRNA. Translation: BAE26570.1.
AK146800 mRNA. Translation: BAE27442.1.
AK150381 mRNA. Translation: BAE29512.1.
AK151900 mRNA. Translation: BAE30783.1.
AK162099 mRNA. Translation: BAE36724.1.
AK167128 mRNA. Translation: BAE39275.1.
BC050891 mRNA. Translation: AAH50891.1.
BC089334 mRNA. Translation: AAH89334.1.
CCDSiCCDS27432.1.
PIRiJC5384.
RefSeqiNP_001240734.1. NM_001253805.1.
NP_001240735.1. NM_001253806.1.
NP_035870.1. NM_011740.3.
XP_011243654.1. XM_011245352.2.
UniGeneiMm.3360.

Genome annotation databases

EnsembliENSMUST00000022894; ENSMUSP00000022894; ENSMUSG00000022285.
ENSMUST00000110361; ENSMUSP00000105990; ENSMUSG00000022285.
ENSMUST00000110362; ENSMUSP00000105991; ENSMUSG00000022285.
GeneIDi22631.
KEGGimmu:22631.
UCSCiuc007vmz.2. mouse.

Similar proteinsi

Entry informationi

Entry namei1433Z_MOUSE
AccessioniPrimary (citable) accession number: P63101
Secondary accession number(s): P35215
, P70197, P97286, Q3TSF1, Q5EBQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: September 27, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families