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Reviewed, UniProtKB/Swiss-Prot P63100 (CANB1_RAT)

Last modified February 9, 2010. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Calcineurin subunit B type 1
Alternative name(s):
    Protein phosphatase 2B regulatory subunit 1
    Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene names
Name: Ppp3r1
Synonyms: Cna2, Cnb
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase, AND MASS SPECTROMETRY. Confers calcium sensitivity.

Subunit structure

Composed of a catalytic subunit (A) and a regulatory subunit (B).

Tissue specificity

Isoform 2 is testis specific. Ref.2

Miscellaneous

This protein has four functional calcium-binding sites.

Sequence similarities

Belongs to the calcineurin regulatory subunit family.

Contains 4 EF-hand domains.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P63100-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P63100-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MG → MEQGTDLQSQIFFPTEKNFWKKGKDHFRQNKYPFSRELYNLIFADRKG

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 170169Calcineurin subunit B type 1
PRO_0000073486

Regions

Domain18 – 5134EF-hand 1
Domain50 – 8536EF-hand 2
Domain87 – 12236EF-hand 3
Domain128 – 16336EF-hand 4
Calcium binding31 – 42121
Calcium binding63 – 74122
Calcium binding100 – 111123
Calcium binding141 – 152124

Amino acid modifications

Modified residue1061Phosphotyrosine By similarity
Lipidation21N-myristoyl glycine Ref.4

Natural variations

Alternative sequence1 – 22MG → MEQGTDLQSQIFFPTEKNFW KKGKDHFRQNKYPFSRELYN LIFADRKG in isoform 2.
VSP_000729

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: C904715DC0386056

FASTA17019,300
        10         20         30         40         50         60 
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID 

        70         80         90        100        110        120 
IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV 

       130        140        150        160        170 
GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

« Hide

Isoform 2 [UniParc] [UniParc].

Checksum: 58B92B78438FDAD0
Show »

FASTA21624,979

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References

« Hide 'large scale' references
[1]"Regulation of calcineurin phosphatase activity by the B subunit and carboxy-terminal inhibitory domains of the A subunit."
Perrino B.A., Huang X., Ng L.Y., Soderling T.R.
Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Fischer.
[2]"cDNA cloning of an alternatively spliced isoform of the regulatory subunit of Ca2+/calmodulin-dependent protein phosphatase (calcineurin B alpha 2)."
Chang C.-D., Mukai H., Kuno T., Tanaka C.
Biochim. Biophys. Acta 1217:174-180(1994) [PubMed: 8110831] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Tissue: Brain and Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]Lubec G., Chen W.-Q.
Submitted (FEB-2007) to UniProtKB
Cited for: MYRISTOYLATION AT GLY-2, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L03554 mRNA. Translation: AAA40854.1.
D14568 mRNA. Translation: BAA03422.1.
D14425 mRNA. Translation: BAA03318.1.
BC088855 mRNA. Translation: AAH88855.1.
IPIIPI00206406.
IPI00230947.
PIRS42716.
RefSeqNP_059005.1.
UniGeneRn.42903

3D structure databases

SMRP63100. Positions 2-170.
ModBaseSearch...

Protein-protein interaction databases

STRINGP63100.

Genome annotation databases

EnsemblENSRNOT00000067846; ENSRNOP00000058834; ENSRNOG00000043210; Rattus norvegicus. [Genome view]
GeneID29748.
KEGGrno:29748.
UCSCD14425. rat.

Organism-specific databases

CTD29748.
RGD69230. Ppp3r1.

Phylogenomic databases

eggNOGroNOG10668.
HOVERGENP63100.
OMAIYDIDKD.
PhylomeDBP63100.

Gene expression databases

ArrayExpressP63100.
GenevestigatorP63100.
GermOnlineENSRNOG00000022857. Rattus norvegicus.

Family and domain databases

InterProIPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
IPR008080. Parvalbumin.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PRINTSPR01697. PARVALBUMIN.
SMARTSM00054. EFh. 4 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio610269.

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Entry information

Entry nameCANB1_RAT
AccessionPrimary (citable) accession number: P63100
Secondary accession number(s): P06705, P15117, Q08044
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: February 9, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents