P63100 (CANB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcineurin subunit B type 1

Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1

Gene names

Name:	Ppp3r1
Synonyms:	Cna2, Cnb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase, AND MASS SPECTROMETRY. Confers calcium sensitivity.
Subunit structure	Composed of a catalytic subunit (A) and a regulatory subunit (B).
Tissue specificity	Isoform 2 is testis specific. Ref.2
Miscellaneous	This protein has four functional calcium-binding sites.
Sequence similarities	Belongs to the calcineurin regulatory subunit family. Contains 4 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein dephosphorylation Inferred from direct assay. Source: RGD
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphoprotein phosphatase activity Inferred from direct assay. Source: RGD protein binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P63100-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P63100-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MG → MEQGTDLQSQIFFPTEKNFWKKGKDHFRQNKYPFSRELYNLIFADRKG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 170	169	Calcineurin subunit B type 1		PRO_0000073486
Regions
	Domain	18 – 51	34	EF-hand 1
	Domain	50 – 85	36	EF-hand 2
	Domain	87 – 122	36	EF-hand 3
	Domain	128 – 163	36	EF-hand 4
	Calcium binding	31 – 42	12	1
	Calcium binding	63 – 74	12	2
	Calcium binding	100 – 111	12	3
	Calcium binding	141 – 152	12	4
Amino acid modifications
	Modified residue	106	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine Ref.4
Natural variations
	Alternative sequence	1 – 2	2	MG → MEQGTDLQSQIFFPTEKNFW KKGKDHFRQNKYPFSRELYN LIFADRKG in isoform 2.		VSP_000729

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C904715DC0386056	FASTA	170	19,300
	10 20 30 40 50 60 MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID 70 80 90 100 110 120 IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV 130 140 150 160 170 GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
	Isoform 2 [UniParc] [UniParc]. Checksum: 58B92B78438FDAD0	FASTA	216	24,979
	10 20 30 40 50 60 MEQGTDLQSQ IFFPTEKNFW KKGKDHFRQN KYPFSRELYN LIFADRKGNE ASYPLEMCSH 70 80 90 100 110 120 FDADEIKRLG KRFKKLDLDN SGSLSVEEFM SLPELQQNPL VQRVIDIFDT DGNGEVDFKE 130 140 150 160 170 180 FIEGVSQFSV KGDKEQKLRF AFRIYDMDKD GYISNGELFQ VLKMMVGNNL KDTQLQQIVD 190 200 210 KTIINADKDG DGRISFEEFC AVVGGLDIHK KMVVDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of calcineurin phosphatase activity by the B subunit and carboxy-terminal inhibitory domains of the A subunit." Perrino B.A., Huang X., Ng L.Y., Soderling T.R. Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Fischer.
[2]	"cDNA cloning of an alternatively spliced isoform of the regulatory subunit of Ca2+/calmodulin-dependent protein phosphatase (calcineurin B alpha 2)." Chang C.-D., Mukai H., Kuno T., Tanaka C. Biochim. Biophys. Acta 1217:174-180(1994) [PubMed: 8110831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Brain and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	Lubec G., Chen W.-Q. Submitted (FEB-2007) to UniProtKB Cited for: MYRISTOYLATION AT GLY-2, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03554 mRNA. Translation: AAA40854.1. D14568 mRNA. Translation: BAA03422.1. D14425 mRNA. Translation: BAA03318.1. BC088855 mRNA. Translation: AAH88855.1.
IPI	IPI00206406. IPI00230947.
PIR	S42716.
RefSeq	NP_059005.1. NM_017309.2.
UniGene	Rn.42903.
3D structure databases
ProteinModelPortal	P63100.
SMR	P63100. Positions 16-170.
ModBase	Search...
Protein-protein interaction databases
IntAct	P63100. 1 interaction.
STRING	P63100.
Proteomic databases
PRIDE	P63100.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000031275; ENSRNOP00000037143; ENSRNOG00000043210. ENSRNOT00000067846; ENSRNOP00000058834; ENSRNOG00000043210.
GeneID	29748.
KEGG	rno:29748.
UCSC	D14425. rat.
Organism-specific databases
CTD	5534.
RGD	69230. Ppp3r1.
Phylogenomic databases
eggNOG	roNOG10668.
GeneTree	ENSGT00600000084053.
HOVERGEN	HBG105307.
OMA	IYDIDKD.
PhylomeDB	P63100.
Gene expression databases
ArrayExpress	P63100.
Genevestigator	P63100.
GermOnline	ENSRNOG00000022857. Rattus norvegicus.
Family and domain databases
InterPro	IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR008080. Parvalbumin. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 3 hits.
KO	K06268.
Pfam	PF00036. efhand. 3 hits. [Graphical view]
PRINTS	PR01697. PARVALBUMIN.
SMART	SM00054. EFh. 4 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 4 hits. PS50222. EF_HAND_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	610269.

Entry information

Entry name

CANB1_RAT

Accession

Primary (citable) accession number: P63100
Secondary accession number(s): P06705, P15117, Q08044

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program