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Protein

Calcineurin subunit B type 1

Gene

PPP3R1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi31 – 421Add BLAST12
Calcium bindingi63 – 742Add BLAST12
Calcium bindingi100 – 1113Add BLAST12
Calcium bindingi141 – 1524Add BLAST12

GO - Molecular functioni

  • calcium-dependent protein serine/threonine phosphatase activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • calmodulin binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-180024. DARPP-32 events.
R-HSA-2871809. FCERI mediated Ca+2 mobilization.
R-HSA-4086398. Ca2+ pathway.
R-HSA-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 1
Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene namesi
Name:PPP3R1
Synonyms:CNA2, CNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9317. PPP3R1.

Subcellular locationi

GO - Cellular componenti

  • calcineurin complex Source: UniProtKB
  • cytosol Source: Reactome
  • mitochondrion Source: GOC
  • nucleoplasm Source: Reactome
  • sarcolemma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5534.
OpenTargetsiENSG00000221823.
PharmGKBiPA33681.

Polymorphism and mutation databases

BioMutaiPPP3R1.
DMDMi52000904.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000734842 – 170Calcineurin subunit B type 1Add BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei106PhosphotyrosineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP63098.
MaxQBiP63098.
PaxDbiP63098.
PeptideAtlasiP63098.
PRIDEiP63098.
TopDownProteomicsiP63098.

2D gel databases

OGPiP63098.

PTM databases

iPTMnetiP63098.
PhosphoSitePlusiP63098.
SwissPalmiP63098.

Expressioni

Gene expression databases

BgeeiENSG00000221823.
CleanExiHS_PPP3R1.
ExpressionAtlasiP63098. baseline and differential.
GenevisibleiP63098. HS.

Organism-specific databases

HPAiCAB005610.
HPA047157.

Interactioni

Subunit structurei

Interacts with CIB1 (via C-terminal region); the interaction increases upon cardiomyocytes hypertrophy (By similarity). Composed of a catalytic subunit (A) and a regulatory subunit (B).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MAP3K5Q996832EBI-915984,EBI-476263
PPP3CAQ082095EBI-915984,EBI-352922

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111526. 35 interactors.
DIPiDIP-6096N.
IntActiP63098. 6 interactors.
MINTiMINT-1339650.
STRINGi9606.ENSP00000234310.

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi12 – 14Combined sources3
Helixi17 – 30Combined sources14
Beta strandi35 – 38Combined sources4
Helixi40 – 44Combined sources5
Helixi47 – 49Combined sources3
Helixi55 – 62Combined sources8
Beta strandi67 – 71Combined sources5
Helixi72 – 80Combined sources9
Beta strandi81 – 85Combined sources5
Helixi88 – 99Combined sources12
Beta strandi104 – 107Combined sources4
Helixi109 – 120Combined sources12
Helixi121 – 123Combined sources3
Helixi126 – 140Combined sources15
Beta strandi144 – 149Combined sources6
Helixi150 – 157Combined sources8
Helixi158 – 160Combined sources3
Helixi162 – 165Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUIX-ray2.10B2-170[»]
1M63X-ray2.80B/F2-170[»]
1MF8X-ray3.10B1-170[»]
2P6BX-ray2.30B/D16-170[»]
3LL8X-ray2.00B/D16-170[»]
4F0ZX-ray1.70B1-170[»]
4OR9X-ray2.23B1-170[»]
4ORAX-ray2.75B1-170[»]
4ORCX-ray2.70B1-170[»]
DisProtiDP00565.
ProteinModelPortaliP63098.
SMRiP63098.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63098.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 51EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini50 – 85EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini87 – 122EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini128 – 163EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00860000133729.
HOVERGENiHBG105307.
InParanoidiP63098.
KOiK06268.
PhylomeDBiP63098.
TreeFamiTF105558.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ
60 70 80 90 100
QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD
110 120 130 140 150
MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF
160 170
EEFCAVVGGL DIHKKMVVDV
Length:170
Mass (Da):19,300
Last modified:January 23, 2007 - v2
Checksum:iC904715DC0386056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30773 mRNA. Translation: AAB08721.1.
CR456938 mRNA. Translation: CAG33219.1.
AK314893 mRNA. Translation: BAG37407.1.
AC017083 Genomic DNA. Translation: AAY14715.1.
CH471053 Genomic DNA. Translation: EAW99878.1.
BC027913 mRNA. Translation: AAH27913.1.
CCDSiCCDS46310.1.
PIRiA33391.
RefSeqiNP_000936.1. NM_000945.3.
UniGeneiHs.280604.

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823.
GeneIDi5534.
KEGGihsa:5534.
UCSCiuc002sei.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30773 mRNA. Translation: AAB08721.1.
CR456938 mRNA. Translation: CAG33219.1.
AK314893 mRNA. Translation: BAG37407.1.
AC017083 Genomic DNA. Translation: AAY14715.1.
CH471053 Genomic DNA. Translation: EAW99878.1.
BC027913 mRNA. Translation: AAH27913.1.
CCDSiCCDS46310.1.
PIRiA33391.
RefSeqiNP_000936.1. NM_000945.3.
UniGeneiHs.280604.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUIX-ray2.10B2-170[»]
1M63X-ray2.80B/F2-170[»]
1MF8X-ray3.10B1-170[»]
2P6BX-ray2.30B/D16-170[»]
3LL8X-ray2.00B/D16-170[»]
4F0ZX-ray1.70B1-170[»]
4OR9X-ray2.23B1-170[»]
4ORAX-ray2.75B1-170[»]
4ORCX-ray2.70B1-170[»]
DisProtiDP00565.
ProteinModelPortaliP63098.
SMRiP63098.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111526. 35 interactors.
DIPiDIP-6096N.
IntActiP63098. 6 interactors.
MINTiMINT-1339650.
STRINGi9606.ENSP00000234310.

PTM databases

iPTMnetiP63098.
PhosphoSitePlusiP63098.
SwissPalmiP63098.

Polymorphism and mutation databases

BioMutaiPPP3R1.
DMDMi52000904.

2D gel databases

OGPiP63098.

Proteomic databases

EPDiP63098.
MaxQBiP63098.
PaxDbiP63098.
PeptideAtlasiP63098.
PRIDEiP63098.
TopDownProteomicsiP63098.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823.
GeneIDi5534.
KEGGihsa:5534.
UCSCiuc002sei.2. human.

Organism-specific databases

CTDi5534.
DisGeNETi5534.
GeneCardsiPPP3R1.
HGNCiHGNC:9317. PPP3R1.
HPAiCAB005610.
HPA047157.
MIMi601302. gene.
neXtProtiNX_P63098.
OpenTargetsiENSG00000221823.
PharmGKBiPA33681.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00860000133729.
HOVERGENiHBG105307.
InParanoidiP63098.
KOiK06268.
PhylomeDBiP63098.
TreeFamiTF105558.

Enzyme and pathway databases

ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-180024. DARPP-32 events.
R-HSA-2871809. FCERI mediated Ca+2 mobilization.
R-HSA-4086398. Ca2+ pathway.
R-HSA-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Miscellaneous databases

ChiTaRSiPPP3R1. human.
EvolutionaryTraceiP63098.
GeneWikiiPPP3R1.
GenomeRNAii5534.
PROiP63098.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000221823.
CleanExiHS_PPP3R1.
ExpressionAtlasiP63098. baseline and differential.
GenevisibleiP63098. HS.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCANB1_HUMAN
AccessioniPrimary (citable) accession number: P63098
Secondary accession number(s): B2RC10
, B5MDU4, P06705, P15117, Q08044, Q53SL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.