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P63098

- CANB1_HUMAN

UniProt

P63098 - CANB1_HUMAN

Protein

Calcineurin subunit B type 1

Gene

PPP3R1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi31 – 42121Add
    BLAST
    Calcium bindingi63 – 74122Add
    BLAST
    Calcium bindingi100 – 111123Add
    BLAST
    Calcium bindingi141 – 152124Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent protein serine/threonine phosphatase activity Source: UniProtKB
    2. calcium ion binding Source: UniProtKB
    3. calmodulin binding Source: UniProtKB
    4. protein binding Source: IntAct
    5. protein domain specific binding Source: BHF-UCL

    GO - Biological processi

    1. apoptotic process Source: Reactome
    2. dephosphorylation Source: GOC
    3. Fc-epsilon receptor signaling pathway Source: Reactome
    4. innate immune response Source: Reactome
    5. intrinsic apoptotic signaling pathway Source: Reactome
    6. positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_15334. DARPP-32 events.
    REACT_163834. FCERI mediated Ca+2 mobilization.
    REACT_172761. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcineurin subunit B type 1
    Alternative name(s):
    Protein phosphatase 2B regulatory subunit 1
    Protein phosphatase 3 regulatory subunit B alpha isoform 1
    Gene namesi
    Name:PPP3R1
    Synonyms:CNA2, CNB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:9317. PPP3R1.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cell membrane By similarity. Cell membranesarcolemma
    Note: Translocates from the cytosol to the sarcolemma in a CIB1-dependent manner during cardiomyocytes hypertrophy.By similarity

    GO - Cellular componenti

    1. calcineurin complex Source: UniProtKB
    2. cytosol Source: Reactome
    3. nucleoplasm Source: Reactome
    4. sarcolemma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33681.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 170169Calcineurin subunit B type 1PRO_0000073484Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Modified residuei106 – 1061PhosphotyrosineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    MaxQBiP63098.
    PaxDbiP63098.
    PRIDEiP63098.

    2D gel databases

    OGPiP63098.

    PTM databases

    PhosphoSiteiP63098.

    Expressioni

    Gene expression databases

    ArrayExpressiP63098.
    BgeeiP63098.
    CleanExiHS_PPP3R1.
    GenevestigatoriP63098.

    Organism-specific databases

    HPAiCAB005610.

    Interactioni

    Subunit structurei

    Interacts with CIB1 (via C-terminal region); the interaction increases upon cardiomyocytes hypertrophy By similarity. Composed of a catalytic subunit (A) and a regulatory subunit (B).By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAP3K5Q996832EBI-915984,EBI-476263
    PPP3CAQ082092EBI-915984,EBI-352922

    Protein-protein interaction databases

    BioGridi111526. 12 interactions.
    DIPiDIP-6096N.
    IntActiP63098. 5 interactions.
    MINTiMINT-1339650.
    STRINGi9606.ENSP00000234310.

    Structurei

    Secondary structure

    1
    170
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 113
    Beta strandi12 – 143
    Helixi17 – 3014
    Beta strandi35 – 384
    Helixi40 – 445
    Helixi47 – 493
    Helixi55 – 628
    Beta strandi67 – 715
    Helixi72 – 809
    Beta strandi81 – 855
    Helixi88 – 9912
    Beta strandi104 – 1074
    Helixi109 – 12012
    Helixi121 – 1233
    Helixi126 – 14015
    Beta strandi144 – 1496
    Helixi150 – 1578
    Helixi158 – 1603
    Helixi162 – 1654

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AUIX-ray2.10B2-170[»]
    1M63X-ray2.80B/F2-170[»]
    1MF8X-ray3.10B1-170[»]
    2P6BX-ray2.30B/D16-170[»]
    3LL8X-ray2.00B/D16-170[»]
    4F0ZX-ray1.70B1-170[»]
    DisProtiDP00565.
    ProteinModelPortaliP63098.
    SMRiP63098. Positions 7-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63098.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 5134EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini87 – 12236EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini128 – 16336EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    HOVERGENiHBG105307.
    KOiK06268.
    PhylomeDBiP63098.
    TreeFamiTF105558.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF00036. EF-hand_1. 2 hits.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 4 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63098-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ    50
    QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD 100
    MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF 150
    EEFCAVVGGL DIHKKMVVDV 170
    Length:170
    Mass (Da):19,300
    Last modified:January 23, 2007 - v2
    Checksum:iC904715DC0386056
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30773 mRNA. Translation: AAB08721.1.
    CR456938 mRNA. Translation: CAG33219.1.
    AK314893 mRNA. Translation: BAG37407.1.
    AC017083 Genomic DNA. Translation: AAY14715.1.
    CH471053 Genomic DNA. Translation: EAW99878.1.
    BC027913 mRNA. Translation: AAH27913.1.
    CCDSiCCDS46310.1.
    PIRiA33391.
    RefSeqiNP_000936.1. NM_000945.3.
    UniGeneiHs.280604.

    Genome annotation databases

    EnsembliENST00000234310; ENSP00000234310; ENSG00000221823.
    GeneIDi5534.
    KEGGihsa:5534.
    UCSCiuc002sei.1. human.

    Polymorphism databases

    DMDMi52000904.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30773 mRNA. Translation: AAB08721.1 .
    CR456938 mRNA. Translation: CAG33219.1 .
    AK314893 mRNA. Translation: BAG37407.1 .
    AC017083 Genomic DNA. Translation: AAY14715.1 .
    CH471053 Genomic DNA. Translation: EAW99878.1 .
    BC027913 mRNA. Translation: AAH27913.1 .
    CCDSi CCDS46310.1.
    PIRi A33391.
    RefSeqi NP_000936.1. NM_000945.3.
    UniGenei Hs.280604.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AUI X-ray 2.10 B 2-170 [» ]
    1M63 X-ray 2.80 B/F 2-170 [» ]
    1MF8 X-ray 3.10 B 1-170 [» ]
    2P6B X-ray 2.30 B/D 16-170 [» ]
    3LL8 X-ray 2.00 B/D 16-170 [» ]
    4F0Z X-ray 1.70 B 1-170 [» ]
    DisProti DP00565.
    ProteinModelPortali P63098.
    SMRi P63098. Positions 7-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111526. 12 interactions.
    DIPi DIP-6096N.
    IntActi P63098. 5 interactions.
    MINTi MINT-1339650.
    STRINGi 9606.ENSP00000234310.

    Chemistry

    BindingDBi P63098.
    DrugBanki DB00337. Pimecrolimus.

    PTM databases

    PhosphoSitei P63098.

    Polymorphism databases

    DMDMi 52000904.

    2D gel databases

    OGPi P63098.

    Proteomic databases

    MaxQBi P63098.
    PaxDbi P63098.
    PRIDEi P63098.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000234310 ; ENSP00000234310 ; ENSG00000221823 .
    GeneIDi 5534.
    KEGGi hsa:5534.
    UCSCi uc002sei.1. human.

    Organism-specific databases

    CTDi 5534.
    GeneCardsi GC02M068358.
    HGNCi HGNC:9317. PPP3R1.
    HPAi CAB005610.
    MIMi 601302. gene.
    neXtProti NX_P63098.
    PharmGKBi PA33681.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5126.
    HOVERGENi HBG105307.
    KOi K06268.
    PhylomeDBi P63098.
    TreeFami TF105558.

    Enzyme and pathway databases

    Reactomei REACT_15334. DARPP-32 events.
    REACT_163834. FCERI mediated Ca+2 mobilization.
    REACT_172761. Ca2+ pathway.

    Miscellaneous databases

    ChiTaRSi PPP3R1. human.
    EvolutionaryTracei P63098.
    GeneWikii PPP3R1.
    GenomeRNAii 5534.
    NextBioi 21438.
    PROi P63098.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63098.
    Bgeei P63098.
    CleanExi HS_PPP3R1.
    Genevestigatori P63098.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF00036. EF-hand_1. 2 hits.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 4 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase."
      Guerini D., Krinks M.H., Sikela J.M., Hahn W.E., Klee C.B.
      DNA 8:675-682(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Blood.
    7. Lubec G., Chen W.-Q., Sun Y.
      Submitted (DEC-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 58-85; 98-117; 126-135 AND 148-164, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Fetal brain cortex.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    9. "Crystal structure of calcineurin-cyclophilin-cyclosporin shows common but distinct recognition of immunophilin-drug complexes."
      Huai Q., Kim H.Y., Liu Y., Zhao Y., Mondragon A., Liu J.O., Ke H.
      Proc. Natl. Acad. Sci. U.S.A. 99:12037-12042(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH PPIA.
    10. "Crystal structure of human calcineurin complexed with cyclosporin A and human cyclophilin."
      Jin L., Harrison S.C.
      Proc. Natl. Acad. Sci. U.S.A. 99:13522-13526(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH PPIA.

    Entry informationi

    Entry nameiCANB1_HUMAN
    AccessioniPrimary (citable) accession number: P63098
    Secondary accession number(s): B2RC10
    , B5MDU4, P06705, P15117, Q08044, Q53SL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This protein has four functional calcium-binding sites.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3