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Protein

Calcineurin subunit B type 1

Gene

PPP3R1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi31 – 42121Add
BLAST
Calcium bindingi63 – 74122Add
BLAST
Calcium bindingi100 – 111123Add
BLAST
Calcium bindingi141 – 152124Add
BLAST

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: UniProtKB
  2. calcium ion binding Source: UniProtKB
  3. calmodulin binding Source: UniProtKB
  4. protein domain specific binding Source: BHF-UCL

GO - Biological processi

  1. apoptotic process Source: Reactome
  2. calcineurin-NFAT signaling cascade Source: BHF-UCL
  3. dephosphorylation Source: GOC
  4. Fc-epsilon receptor signaling pathway Source: Reactome
  5. innate immune response Source: Reactome
  6. intrinsic apoptotic signaling pathway Source: Reactome
  7. positive regulation of NFAT protein import into nucleus Source: BHF-UCL
  8. positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
  9. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
REACT_163834. FCERI mediated Ca+2 mobilization.
REACT_172761. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 1
Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene namesi
Name:PPP3R1
Synonyms:CNA2, CNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:9317. PPP3R1.

Subcellular locationi

Cytoplasmcytosol By similarity. Cell membrane By similarity. Cell membranesarcolemma
Note: Translocates from the cytosol to the sarcolemma in a CIB1-dependent manner during cardiomyocytes hypertrophy.By similarity

GO - Cellular componenti

  1. calcineurin complex Source: UniProtKB
  2. cytosol Source: Reactome
  3. nucleoplasm Source: Reactome
  4. sarcolemma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33681.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 170169Calcineurin subunit B type 1PRO_0000073484Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei106 – 1061PhosphotyrosineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP63098.
PaxDbiP63098.
PRIDEiP63098.

2D gel databases

OGPiP63098.

PTM databases

PhosphoSiteiP63098.

Expressioni

Gene expression databases

BgeeiP63098.
CleanExiHS_PPP3R1.
ExpressionAtlasiP63098. baseline and differential.
GenevestigatoriP63098.

Organism-specific databases

HPAiCAB005610.

Interactioni

Subunit structurei

Interacts with CIB1 (via C-terminal region); the interaction increases upon cardiomyocytes hypertrophy (By similarity). Composed of a catalytic subunit (A) and a regulatory subunit (B).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MAP3K5Q996832EBI-915984,EBI-476263
PPP3CAQ082092EBI-915984,EBI-352922

Protein-protein interaction databases

BioGridi111526. 19 interactions.
DIPiDIP-6096N.
IntActiP63098. 5 interactions.
MINTiMINT-1339650.
STRINGi9606.ENSP00000234310.

Structurei

Secondary structure

1
170
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113Combined sources
Beta strandi12 – 143Combined sources
Helixi17 – 3014Combined sources
Beta strandi35 – 384Combined sources
Helixi40 – 445Combined sources
Helixi47 – 493Combined sources
Helixi55 – 628Combined sources
Beta strandi67 – 715Combined sources
Helixi72 – 809Combined sources
Helixi88 – 9912Combined sources
Beta strandi104 – 1074Combined sources
Helixi109 – 12012Combined sources
Helixi121 – 1233Combined sources
Helixi126 – 14015Combined sources
Beta strandi144 – 1496Combined sources
Helixi150 – 1578Combined sources
Helixi158 – 1603Combined sources
Helixi162 – 1654Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUIX-ray2.10B2-170[»]
1M63X-ray2.80B/F2-170[»]
1MF8X-ray3.10B1-170[»]
2P6BX-ray2.30B/D16-170[»]
3LL8X-ray2.00B/D16-170[»]
4F0ZX-ray1.70B1-170[»]
DisProtiDP00565.
ProteinModelPortaliP63098.
SMRiP63098. Positions 7-161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63098.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 5134EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 12236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini128 – 16336EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00760000119179.
HOVERGENiHBG105307.
InParanoidiP63098.
KOiK06268.
PhylomeDBiP63098.
TreeFamiTF105558.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63098-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ
60 70 80 90 100
QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD
110 120 130 140 150
MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF
160 170
EEFCAVVGGL DIHKKMVVDV
Length:170
Mass (Da):19,300
Last modified:January 23, 2007 - v2
Checksum:iC904715DC0386056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30773 mRNA. Translation: AAB08721.1.
CR456938 mRNA. Translation: CAG33219.1.
AK314893 mRNA. Translation: BAG37407.1.
AC017083 Genomic DNA. Translation: AAY14715.1.
CH471053 Genomic DNA. Translation: EAW99878.1.
BC027913 mRNA. Translation: AAH27913.1.
CCDSiCCDS46310.1.
PIRiA33391.
RefSeqiNP_000936.1. NM_000945.3.
UniGeneiHs.280604.

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823.
GeneIDi5534.
KEGGihsa:5534.
UCSCiuc002sei.1. human.

Polymorphism databases

DMDMi52000904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30773 mRNA. Translation: AAB08721.1.
CR456938 mRNA. Translation: CAG33219.1.
AK314893 mRNA. Translation: BAG37407.1.
AC017083 Genomic DNA. Translation: AAY14715.1.
CH471053 Genomic DNA. Translation: EAW99878.1.
BC027913 mRNA. Translation: AAH27913.1.
CCDSiCCDS46310.1.
PIRiA33391.
RefSeqiNP_000936.1. NM_000945.3.
UniGeneiHs.280604.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUIX-ray2.10B2-170[»]
1M63X-ray2.80B/F2-170[»]
1MF8X-ray3.10B1-170[»]
2P6BX-ray2.30B/D16-170[»]
3LL8X-ray2.00B/D16-170[»]
4F0ZX-ray1.70B1-170[»]
DisProtiDP00565.
ProteinModelPortaliP63098.
SMRiP63098. Positions 7-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111526. 19 interactions.
DIPiDIP-6096N.
IntActiP63098. 5 interactions.
MINTiMINT-1339650.
STRINGi9606.ENSP00000234310.

PTM databases

PhosphoSiteiP63098.

Polymorphism databases

DMDMi52000904.

2D gel databases

OGPiP63098.

Proteomic databases

MaxQBiP63098.
PaxDbiP63098.
PRIDEiP63098.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823.
GeneIDi5534.
KEGGihsa:5534.
UCSCiuc002sei.1. human.

Organism-specific databases

CTDi5534.
GeneCardsiGC02M068405.
HGNCiHGNC:9317. PPP3R1.
HPAiCAB005610.
MIMi601302. gene.
neXtProtiNX_P63098.
PharmGKBiPA33681.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00760000119179.
HOVERGENiHBG105307.
InParanoidiP63098.
KOiK06268.
PhylomeDBiP63098.
TreeFamiTF105558.

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
REACT_163834. FCERI mediated Ca+2 mobilization.
REACT_172761. Ca2+ pathway.

Miscellaneous databases

ChiTaRSiPPP3R1. human.
EvolutionaryTraceiP63098.
GeneWikiiPPP3R1.
GenomeRNAii5534.
NextBioi21438.
PROiP63098.
SOURCEiSearch...

Gene expression databases

BgeeiP63098.
CleanExiHS_PPP3R1.
ExpressionAtlasiP63098. baseline and differential.
GenevestigatoriP63098.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase."
    Guerini D., Krinks M.H., Sikela J.M., Hahn W.E., Klee C.B.
    DNA 8:675-682(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood.
  7. Lubec G., Chen W.-Q., Sun Y.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 58-85; 98-117; 126-135 AND 148-164, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fetal brain cortex.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
  9. "Crystal structure of calcineurin-cyclophilin-cyclosporin shows common but distinct recognition of immunophilin-drug complexes."
    Huai Q., Kim H.Y., Liu Y., Zhao Y., Mondragon A., Liu J.O., Ke H.
    Proc. Natl. Acad. Sci. U.S.A. 99:12037-12042(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH PPIA.
  10. "Crystal structure of human calcineurin complexed with cyclosporin A and human cyclophilin."
    Jin L., Harrison S.C.
    Proc. Natl. Acad. Sci. U.S.A. 99:13522-13526(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH PPIA.

Entry informationi

Entry nameiCANB1_HUMAN
AccessioniPrimary (citable) accession number: P63098
Secondary accession number(s): B2RC10
, B5MDU4, P06705, P15117, Q08044, Q53SL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.