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P63098

- CANB1_HUMAN

UniProt

P63098 - CANB1_HUMAN

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Protein

Calcineurin subunit B type 1

Gene
PPP3R1, CNA2, CNB
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi31 – 42121Add
BLAST
Calcium bindingi63 – 74122Add
BLAST
Calcium bindingi100 – 111123Add
BLAST
Calcium bindingi141 – 152124Add
BLAST

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: UniProtKB
  2. calcium ion binding Source: UniProtKB
  3. calmodulin binding Source: UniProtKB
  4. protein binding Source: IntAct
  5. protein domain specific binding Source: BHF-UCL

GO - Biological processi

  1. apoptotic process Source: Reactome
  2. dephosphorylation Source: GOC
  3. Fc-epsilon receptor signaling pathway Source: Reactome
  4. innate immune response Source: Reactome
  5. intrinsic apoptotic signaling pathway Source: Reactome
  6. positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
REACT_163834. FCERI mediated Ca+2 mobilization.
REACT_172761. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 1
Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene namesi
Name:PPP3R1
Synonyms:CNA2, CNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:9317. PPP3R1.

Subcellular locationi

Cytoplasmcytosol By similarity. Cell membrane By similarity. Cell membranesarcolemma
Note: Translocates from the cytosol to the sarcolemma in a CIB1-dependent manner during cardiomyocytes hypertrophy By similarity.

GO - Cellular componenti

  1. calcineurin complex Source: UniProtKB
  2. cytosol Source: Reactome
  3. nucleoplasm Source: Reactome
  4. sarcolemma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33681.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 170169Calcineurin subunit B type 1PRO_0000073484Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Modified residuei106 – 1061Phosphotyrosine By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP63098.
PaxDbiP63098.
PRIDEiP63098.

2D gel databases

OGPiP63098.

PTM databases

PhosphoSiteiP63098.

Expressioni

Gene expression databases

ArrayExpressiP63098.
BgeeiP63098.
CleanExiHS_PPP3R1.
GenevestigatoriP63098.

Organism-specific databases

HPAiCAB005610.

Interactioni

Subunit structurei

Interacts with CIB1 (via C-terminal region); the interaction increases upon cardiomyocytes hypertrophy By similarity. Composed of a catalytic subunit (A) and a regulatory subunit (B).

Binary interactionsi

WithEntry#Exp.IntActNotes
MAP3K5Q996832EBI-915984,EBI-476263
PPP3CAQ082092EBI-915984,EBI-352922

Protein-protein interaction databases

BioGridi111526. 12 interactions.
DIPiDIP-6096N.
IntActiP63098. 5 interactions.
MINTiMINT-1339650.
STRINGi9606.ENSP00000234310.

Structurei

Secondary structure

1
170
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Beta strandi12 – 143
Helixi17 – 3014
Beta strandi35 – 384
Helixi40 – 445
Helixi47 – 493
Helixi55 – 628
Beta strandi67 – 715
Helixi72 – 809
Beta strandi81 – 855
Helixi88 – 9912
Beta strandi104 – 1074
Helixi109 – 12012
Helixi121 – 1233
Helixi126 – 14015
Beta strandi144 – 1496
Helixi150 – 1578
Helixi158 – 1603
Helixi162 – 1654

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUIX-ray2.10B2-170[»]
1M63X-ray2.80B/F2-170[»]
1MF8X-ray3.10B1-170[»]
2P6BX-ray2.30B/D16-170[»]
3LL8X-ray2.00B/D16-170[»]
4F0ZX-ray1.70B1-170[»]
DisProtiDP00565.
ProteinModelPortaliP63098.
SMRiP63098. Positions 7-161.

Miscellaneous databases

EvolutionaryTraceiP63098.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 5134EF-hand 1Add
BLAST
Domaini50 – 8536EF-hand 2Add
BLAST
Domaini87 – 12236EF-hand 3Add
BLAST
Domaini128 – 16336EF-hand 4Add
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
HOVERGENiHBG105307.
KOiK06268.
PhylomeDBiP63098.
TreeFamiTF105558.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63098-1 [UniParc]FASTAAdd to Basket

« Hide

MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ    50
QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD 100
MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF 150
EEFCAVVGGL DIHKKMVVDV 170
Length:170
Mass (Da):19,300
Last modified:January 23, 2007 - v2
Checksum:iC904715DC0386056
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30773 mRNA. Translation: AAB08721.1.
CR456938 mRNA. Translation: CAG33219.1.
AK314893 mRNA. Translation: BAG37407.1.
AC017083 Genomic DNA. Translation: AAY14715.1.
CH471053 Genomic DNA. Translation: EAW99878.1.
BC027913 mRNA. Translation: AAH27913.1.
CCDSiCCDS46310.1.
PIRiA33391.
RefSeqiNP_000936.1. NM_000945.3.
UniGeneiHs.280604.

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823.
GeneIDi5534.
KEGGihsa:5534.
UCSCiuc002sei.1. human.

Polymorphism databases

DMDMi52000904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30773 mRNA. Translation: AAB08721.1 .
CR456938 mRNA. Translation: CAG33219.1 .
AK314893 mRNA. Translation: BAG37407.1 .
AC017083 Genomic DNA. Translation: AAY14715.1 .
CH471053 Genomic DNA. Translation: EAW99878.1 .
BC027913 mRNA. Translation: AAH27913.1 .
CCDSi CCDS46310.1.
PIRi A33391.
RefSeqi NP_000936.1. NM_000945.3.
UniGenei Hs.280604.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AUI X-ray 2.10 B 2-170 [» ]
1M63 X-ray 2.80 B/F 2-170 [» ]
1MF8 X-ray 3.10 B 1-170 [» ]
2P6B X-ray 2.30 B/D 16-170 [» ]
3LL8 X-ray 2.00 B/D 16-170 [» ]
4F0Z X-ray 1.70 B 1-170 [» ]
DisProti DP00565.
ProteinModelPortali P63098.
SMRi P63098. Positions 7-161.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111526. 12 interactions.
DIPi DIP-6096N.
IntActi P63098. 5 interactions.
MINTi MINT-1339650.
STRINGi 9606.ENSP00000234310.

Chemistry

BindingDBi P63098.
DrugBanki DB00337. Pimecrolimus.

PTM databases

PhosphoSitei P63098.

Polymorphism databases

DMDMi 52000904.

2D gel databases

OGPi P63098.

Proteomic databases

MaxQBi P63098.
PaxDbi P63098.
PRIDEi P63098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000234310 ; ENSP00000234310 ; ENSG00000221823 .
GeneIDi 5534.
KEGGi hsa:5534.
UCSCi uc002sei.1. human.

Organism-specific databases

CTDi 5534.
GeneCardsi GC02M068358.
HGNCi HGNC:9317. PPP3R1.
HPAi CAB005610.
MIMi 601302. gene.
neXtProti NX_P63098.
PharmGKBi PA33681.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5126.
HOVERGENi HBG105307.
KOi K06268.
PhylomeDBi P63098.
TreeFami TF105558.

Enzyme and pathway databases

Reactomei REACT_15334. DARPP-32 events.
REACT_163834. FCERI mediated Ca+2 mobilization.
REACT_172761. Ca2+ pathway.

Miscellaneous databases

ChiTaRSi PPP3R1. human.
EvolutionaryTracei P63098.
GeneWikii PPP3R1.
GenomeRNAii 5534.
NextBioi 21438.
PROi P63098.
SOURCEi Search...

Gene expression databases

ArrayExpressi P63098.
Bgeei P63098.
CleanExi HS_PPP3R1.
Genevestigatori P63098.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF00036. EF-hand_1. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 4 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase."
    Guerini D., Krinks M.H., Sikela J.M., Hahn W.E., Klee C.B.
    DNA 8:675-682(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood.
  7. Lubec G., Chen W.-Q., Sun Y.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 58-85; 98-117; 126-135 AND 148-164, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fetal brain cortex.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
  9. "Crystal structure of calcineurin-cyclophilin-cyclosporin shows common but distinct recognition of immunophilin-drug complexes."
    Huai Q., Kim H.Y., Liu Y., Zhao Y., Mondragon A., Liu J.O., Ke H.
    Proc. Natl. Acad. Sci. U.S.A. 99:12037-12042(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH PPIA.
  10. "Crystal structure of human calcineurin complexed with cyclosporin A and human cyclophilin."
    Jin L., Harrison S.C.
    Proc. Natl. Acad. Sci. U.S.A. 99:13522-13526(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH PPIA.

Entry informationi

Entry nameiCANB1_HUMAN
AccessioniPrimary (citable) accession number: P63098
Secondary accession number(s): B2RC10
, B5MDU4, P06705, P15117, Q08044, Q53SL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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