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P63096

- GNAI1_HUMAN

UniProt

P63096 - GNAI1_HUMAN

Protein

Guanine nucleotide-binding protein G(i) subunit alpha-1

Gene

GNAI1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumBy similarity
    Metal bindingi181 – 1811MagnesiumBy similarity
    Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 478GTP
    Nucleotide bindingi175 – 1817GTPBy similarity
    Nucleotide bindingi200 – 2045GTP
    Nucleotide bindingi269 – 2724GTP

    GO - Molecular functioni

    1. G-protein beta/gamma-subunit complex binding Source: RefGenome
    2. G-protein coupled serotonin receptor binding Source: RefGenome
    3. GTPase activity Source: RefGenome
    4. GTP binding Source: BHF-UCL
    5. metal ion binding Source: UniProtKB-KW
    6. protein binding Source: IntAct
    7. signal transducer activity Source: RefGenome

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: Reactome
    2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    3. blood coagulation Source: Reactome
    4. cell cycle Source: UniProtKB-KW
    5. cell division Source: UniProtKB
    6. G-protein coupled receptor signaling pathway Source: BHF-UCL
    7. platelet activation Source: Reactome
    8. response to peptide hormone Source: BHF-UCL
    9. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Transducer

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_15333. Adenylate cyclase inhibitory pathway.
    REACT_15426. PLC beta mediated events.
    REACT_15457. G-protein activation.
    REACT_18325. Regulation of insulin secretion.
    REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_19231. G alpha (i) signalling events.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    SignaLinkiP63096.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(i) subunit alpha-1
    Alternative name(s):
    Adenylate cyclase-inhibiting G alpha protein
    Gene namesi
    Name:GNAI1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:4384. GNAI1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm 1 Publication. Cell membrane 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication
    Note: Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane By similarity. Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. extracellular vesicular exosome Source: UniProt
    4. heterotrimeric G-protein complex Source: RefGenome
    5. lysosomal membrane Source: UniProtKB
    6. midbody Source: UniProtKB
    7. nucleus Source: UniProtKB-SubCell
    8. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi116 – 1161E → L: Enhances interaction (inactive GDP-bound) with RGS14. 2 Publications
    Mutagenesisi147 – 1471Q → L: Enhances interaction (inactive GDP-bound) with RGS14. 2 Publications
    Mutagenesisi245 – 2451E → L: Enhances interaction (inactive GDP-bound) with RGS14. 1 Publication

    Organism-specific databases

    PharmGKBiPA172.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 354353Guanine nucleotide-binding protein G(i) subunit alpha-1PRO_0000203671Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity
    Modified residuei178 – 1781ADP-ribosylarginine; by cholera toxinBy similarity
    Modified residuei351 – 3511ADP-ribosylcysteine; by pertussis toxinBy similarity

    Keywords - PTMi

    ADP-ribosylation, Lipoprotein, Myristate, Palmitate

    Proteomic databases

    MaxQBiP63096.
    PaxDbiP63096.
    PRIDEiP63096.

    PTM databases

    PhosphoSiteiP63096.

    Expressioni

    Gene expression databases

    ArrayExpressiP63096.
    BgeeiP63096.
    CleanExiHS_GNAI1.
    GenevestigatoriP63096.

    Organism-specific databases

    HPAiCAB022449.

    Interactioni

    Subunit structurei

    Interacts with GPSM1. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains); the interaction occurs in the centrosomes. Interacts (GDP-bound form) with RIC8A (via C-terminus) By similarity. Interacts with DRD2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GPSM2P812743EBI-618639,EBI-618655
    NUMA1Q149804EBI-618639,EBI-521611

    Protein-protein interaction databases

    BioGridi109032. 48 interactions.
    IntActiP63096. 19 interactions.
    MINTiMINT-2913925.
    STRINGi9606.ENSP00000343027.

    Structurei

    Secondary structure

    1
    354
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 168
    Helixi20 – 234
    Helixi27 – 293
    Beta strandi31 – 4111
    Helixi46 – 5712
    Helixi63 – 675
    Helixi70 – 9122
    Helixi100 – 11314
    Beta strandi115 – 1173
    Helixi121 – 13212
    Helixi134 – 1407
    Helixi141 – 1455
    Helixi152 – 1576
    Helixi159 – 1624
    Beta strandi164 – 1663
    Helixi171 – 1755
    Beta strandi183 – 1919
    Beta strandi194 – 2018
    Helixi208 – 2158
    Beta strandi220 – 2267
    Helixi227 – 2293
    Beta strandi238 – 2414
    Helixi242 – 25413
    Helixi257 – 2593
    Beta strandi260 – 26910
    Helixi271 – 2777
    Turni278 – 2803
    Helixi283 – 2853
    Helixi296 – 30813
    Turni314 – 3163
    Beta strandi319 – 3235
    Helixi329 – 34618
    Helixi348 – 3503

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KJYX-ray2.70A/C30-354[»]
    1Y3AX-ray2.50A/B/C/D26-354[»]
    2G83X-ray2.80A/B33-345[»]
    2GTPX-ray2.55A/B32-354[»]
    2IK8X-ray2.71A/C31-354[»]
    2OM2X-ray2.20A/C31-354[»]
    2PZ3X-ray2.42A1-354[»]
    2XNSX-ray3.41A/B30-354[»]
    3ONWX-ray2.38A/B31-354[»]
    3QE0X-ray3.00A/B/C33-354[»]
    3QI2X-ray2.80A/B31-354[»]
    3UMRX-ray2.24A1-354[»]
    3UMSX-ray2.60A1-354[»]
    4G5QX-ray2.90A/B/C/D25-354[»]
    ProteinModelPortaliP63096.
    SMRiP63096. Positions 5-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63096.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG322962.
    HOVERGENiHBG063184.
    InParanoidiP63096.
    KOiK04630.
    OMAiECEEYRR.
    PhylomeDBiP63096.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P63096-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGCTLSAEDK AAVERSKMID RNLREDGEKA AREVKLLLLG AGESGKSTIV    50
    KQMKIIHEAG YSEEECKQYK AVVYSNTIQS IIAIIRAMGR LKIDFGDSAR 100
    ADDARQLFVL AGAAEEGFMT AELAGVIKRL WKDSGVQACF NRSREYQLND 150
    SAAYYLNDLD RIAQPNYIPT QQDVLRTRVK TTGIVETHFT FKDLHFKMFD 200
    VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF 250
    DSICNNKWFT DTSIILFLNK KDLFEEKIKK SPLTICYPEY AGSNTYEEAA 300
    AYIQCQFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD 350
    CGLF 354
    Length:354
    Mass (Da):40,361
    Last modified:January 23, 2007 - v2
    Checksum:i9F88311B46E62DE3
    GO
    Isoform 2 (identifier: P63096-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-52: Missing.

    Show »
    Length:302
    Mass (Da):34,775
    Checksum:iDA5ED62E61B790A9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti138 – 1381A → G in CAB43212. (PubMed:11230166)Curated
    Sequence conflicti219 – 2191T → A in AAH26326. (PubMed:15489334)Curated
    Sequence conflicti244 – 2441H → Y in AAV38580. 1 PublicationCurated
    Sequence conflicti249 – 2491L → M in AAV38580. 1 PublicationCurated
    Sequence conflicti288 – 2881P → Q in AAH26326. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5252Missing in isoform 2. 1 PublicationVSP_045215Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF493905 mRNA. Translation: AAM12619.1.
    AF055013 mRNA. Translation: AAC09361.1.
    AL049933 mRNA. Translation: CAB43212.2.
    BT019775 mRNA. Translation: AAV38580.1.
    AK292953 mRNA. Translation: BAF85642.1.
    AK304442 mRNA. Translation: BAG65263.1.
    AC004159 Genomic DNA. No translation available.
    AC080066 Genomic DNA. No translation available.
    CH471091 Genomic DNA. Translation: EAW77011.1.
    BC026326 mRNA. Translation: AAH26326.1.
    M20596, M20594, M20595 Genomic DNA. Translation: AAA35893.1.
    M17219 mRNA. Translation: AAA52581.1.
    CCDSiCCDS5595.1. [P63096-1]
    CCDS59061.1. [P63096-2]
    PIRiA28318. RGHUI1.
    RefSeqiNP_001243343.1. NM_001256414.1. [P63096-2]
    NP_002060.4. NM_002069.5. [P63096-1]
    UniGeneiHs.134587.

    Genome annotation databases

    EnsembliENST00000351004; ENSP00000343027; ENSG00000127955. [P63096-1]
    ENST00000457358; ENSP00000410572; ENSG00000127955. [P63096-2]
    GeneIDi2770.
    KEGGihsa:2770.
    UCSCiuc003uhb.1. human. [P63096-1]

    Polymorphism databases

    DMDMi52000964.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF493905 mRNA. Translation: AAM12619.1 .
    AF055013 mRNA. Translation: AAC09361.1 .
    AL049933 mRNA. Translation: CAB43212.2 .
    BT019775 mRNA. Translation: AAV38580.1 .
    AK292953 mRNA. Translation: BAF85642.1 .
    AK304442 mRNA. Translation: BAG65263.1 .
    AC004159 Genomic DNA. No translation available.
    AC080066 Genomic DNA. No translation available.
    CH471091 Genomic DNA. Translation: EAW77011.1 .
    BC026326 mRNA. Translation: AAH26326.1 .
    M20596 , M20594 , M20595 Genomic DNA. Translation: AAA35893.1 .
    M17219 mRNA. Translation: AAA52581.1 .
    CCDSi CCDS5595.1. [P63096-1 ]
    CCDS59061.1. [P63096-2 ]
    PIRi A28318. RGHUI1.
    RefSeqi NP_001243343.1. NM_001256414.1. [P63096-2 ]
    NP_002060.4. NM_002069.5. [P63096-1 ]
    UniGenei Hs.134587.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KJY X-ray 2.70 A/C 30-354 [» ]
    1Y3A X-ray 2.50 A/B/C/D 26-354 [» ]
    2G83 X-ray 2.80 A/B 33-345 [» ]
    2GTP X-ray 2.55 A/B 32-354 [» ]
    2IK8 X-ray 2.71 A/C 31-354 [» ]
    2OM2 X-ray 2.20 A/C 31-354 [» ]
    2PZ3 X-ray 2.42 A 1-354 [» ]
    2XNS X-ray 3.41 A/B 30-354 [» ]
    3ONW X-ray 2.38 A/B 31-354 [» ]
    3QE0 X-ray 3.00 A/B/C 33-354 [» ]
    3QI2 X-ray 2.80 A/B 31-354 [» ]
    3UMR X-ray 2.24 A 1-354 [» ]
    3UMS X-ray 2.60 A 1-354 [» ]
    4G5Q X-ray 2.90 A/B/C/D 25-354 [» ]
    ProteinModelPortali P63096.
    SMRi P63096. Positions 5-348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109032. 48 interactions.
    IntActi P63096. 19 interactions.
    MINTi MINT-2913925.
    STRINGi 9606.ENSP00000343027.

    Chemistry

    BindingDBi P63096.
    ChEMBLi CHEMBL4741.

    PTM databases

    PhosphoSitei P63096.

    Polymorphism databases

    DMDMi 52000964.

    Proteomic databases

    MaxQBi P63096.
    PaxDbi P63096.
    PRIDEi P63096.

    Protocols and materials databases

    DNASUi 2770.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000351004 ; ENSP00000343027 ; ENSG00000127955 . [P63096-1 ]
    ENST00000457358 ; ENSP00000410572 ; ENSG00000127955 . [P63096-2 ]
    GeneIDi 2770.
    KEGGi hsa:2770.
    UCSCi uc003uhb.1. human. [P63096-1 ]

    Organism-specific databases

    CTDi 2770.
    GeneCardsi GC07P079763.
    HGNCi HGNC:4384. GNAI1.
    HPAi CAB022449.
    MIMi 139310. gene.
    neXtProti NX_P63096.
    PharmGKBi PA172.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG322962.
    HOVERGENi HBG063184.
    InParanoidi P63096.
    KOi K04630.
    OMAi ECEEYRR.
    PhylomeDBi P63096.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_15333. Adenylate cyclase inhibitory pathway.
    REACT_15426. PLC beta mediated events.
    REACT_15457. G-protein activation.
    REACT_18325. Regulation of insulin secretion.
    REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_19231. G alpha (i) signalling events.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    SignaLinki P63096.

    Miscellaneous databases

    EvolutionaryTracei P63096.
    GeneWikii GNAI1.
    GenomeRNAii 2770.
    NextBioi 10896.
    PROi P63096.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63096.
    Bgeei P63096.
    CleanExi HS_GNAI1.
    Genevestigatori P63096.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    2. Yu W., Gibbs R.A.
      Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Trachea.
    6. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    9. "Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs."
      Itoh H., Toyama R., Kozasa T., Tsukamoto T., Matsuoka M., Kaziro Y.
      J. Biol. Chem. 263:6656-6664(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-101.
    10. "Human cDNA clones for an alpha subunit of Gi signal-transduction protein."
      Bray P., Carter A., Guo V., Puckett C., Kamholz J., Spiegel A., Nirenberg M.
      Proc. Natl. Acad. Sci. U.S.A. 84:5115-5119(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-354.
    11. "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes."
      Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V.
      Biochem. J. 383:237-248(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 91-100 AND 162-176.
      Tissue: Adipocyte.
    12. "Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division."
      Cho H., Kehrl J.H.
      J. Cell Biol. 178:245-255(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    13. "Strategy for comprehensive identification of human N-myristoylated proteins using an insect cell-free protein synthesis system."
      Suzuki T., Moriya K., Nagatoshi K., Ota Y., Ezure T., Ando E., Tsunasawa S., Utsumi T.
      Proteomics 10:1780-1793(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: MYRISTOYLATION AT GLY-2.
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits."
      Kimple R.J., Kimple M.E., Betts L., Sondek J., Siderovski D.P.
      Nature 416:878-881(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 31-349 IN COMPLEX WITH RGS14 AND GDP.
    16. "Structure of Galpha(i1) bound to a GDP-selective peptide provides insight into guanine nucleotide exchange."
      Johnston C.A., Willard F.S., Jezyk M.R., Fredericks Z., Bodor E.T., Jones M.B., Blaesius R., Watts V.J., Harden T.K., Sondek J., Ramer J.K., Siderovski D.P.
      Structure 13:1069-1080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 26-354 IN COMPLEX WITH GDP.
    17. "Structure-based protocol for identifying mutations that enhance protein-protein binding affinities."
      Sammond D.W., Eletr Z.M., Purbeck C., Kimple R.J., Siderovski D.P., Kuhlman B.
      J. Mol. Biol. 371:1392-1404(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 31-354, MUTAGENESIS OF GLU-116 AND GLN-147.
    18. "Structural basis for nucleotide exchange on G alpha i subunits and receptor coupling specificity."
      Johnston C.A., Siderovski D.P.
      Proc. Natl. Acad. Sci. U.S.A. 104:2001-2006(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH DRD2 AND GDP, FUNCTION, INTERACTION WITH DRD2.
    19. Cited for: RETRACTION.
    20. "Structural determinants of affinity enhancement between GoLoco motifs and G-protein alpha subunit mutants."
      Bosch D.E., Kimple A.J., Sammond D.W., Muller R.E., Miley M.J., Machius M., Kuhlman B., Willard F.S., Siderovski D.P.
      J. Biol. Chem. 286:3351-3358(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 31-354 IN COMPLEX WITH RGS14 AND GDP, MUTAGENESIS OF GLU-116; GLN-147 AND GLU-245.

    Entry informationi

    Entry nameiGNAI1_HUMAN
    AccessioniPrimary (citable) accession number: P63096
    Secondary accession number(s): A8KA88
    , B4E2V1, C9J3A4, P04898, P11015, P31871, Q5U074, Q8TAN5, Q9UGA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3