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Protein

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Gene

Gnas

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541MagnesiumBy similarity
Metal bindingi204 – 2041MagnesiumBy similarity
Binding sitei366 – 3661GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 559GTPBy similarity
Nucleotide bindingi197 – 2048GTPBy similarity
Nucleotide bindingi223 – 2275GTPBy similarity
Nucleotide bindingi292 – 2954GTPBy similarity

GO - Molecular functioni

  • alkylglycerophosphoethanolamine phosphodiesterase activity Source: MGI
  • alpha-tubulin binding Source: RGD
  • beta-2 adrenergic receptor binding Source: RGD
  • corticotropin-releasing hormone receptor 1 binding Source: RGD
  • D1 dopamine receptor binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein beta-subunit binding Source: RGD
  • GTPase activity Source: InterPro
  • GTP binding Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • ionotropic glutamate receptor binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • mu-type opioid receptor binding Source: RGD
  • protein domain specific binding Source: RGD
  • signal transducer activity Source: RGD

GO - Biological processi

  • adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
  • adenylate cyclase-activating dopamine receptor signaling pathway Source: BHF-UCL
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • calcium ion homeostasis Source: RGD
  • cellular response to catecholamine stimulus Source: BHF-UCL
  • cellular response to prostaglandin E stimulus Source: BHF-UCL
  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of renal sodium excretion Source: RGD
  • positive regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of cAMP-mediated signaling Source: UniProtKB
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of sodium ion transport Source: RGD
  • regulation of insulin secretion Source: Reactome
  • regulation of transcription from RNA polymerase II promoter Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein
G-alpha-8
Gene namesi
Name:Gnas
Synonyms:Gnas1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2716. Gnas.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: BHF-UCL
  • endosome Source: RGD
  • heterotrimeric G-protein complex Source: BHF-UCL
  • membrane Source: BHF-UCL
  • membrane raft Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • ruffle Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi3 – 31C → A: Abolishes S-palmitoylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortPRO_0000203725Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-palmitoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteine1 Publication
Cross-linki300 – 300Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei352 – 3521PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP63095.

PTM databases

iPTMnetiP63095.
PhosphoSiteiP63095.
SwissPalmiP63095.

Expressioni

Tissue specificityi

Isoform Gnas-3 is abundant in brain with intermediate levels in skeletal muscle and very low levels in other tissues.1 Publication

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (By similarity). Interacts with CRY1; the interaction may block GPCR-mediated regulation of cAMP concentrations. Interacts with ADCY6 and stimulates its adenylyl cyclase activity (By similarity). Interacts with ADCY2 and ADCY5 (By similarity). Stimulates the ADCY5 adenylyl cyclase activity (By similarity).By similarity

GO - Molecular functioni

  • alpha-tubulin binding Source: RGD
  • beta-2 adrenergic receptor binding Source: RGD
  • corticotropin-releasing hormone receptor 1 binding Source: RGD
  • D1 dopamine receptor binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein beta-subunit binding Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • ionotropic glutamate receptor binding Source: RGD
  • mu-type opioid receptor binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi247005. 1 interaction.
IntActiP63095. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP63095.
SMRiP63095. Positions 9-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

HOVERGENiHBG063184.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 2 hits.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gnas-1 (identifier: P63095-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE
60 70 80 90 100
SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK
110 120 130 140 150
EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPNF DFPPEFYEHA
160 170 180 190 200
KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQADY VPSDQDLLRC
210 220 230 240 250
RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVAS
260 270 280 290 300
SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK
310 320 330 340 350
VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST
360 370 380 390
ASGDGRHYCY PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL
Length:394
Mass (Da):45,664
Last modified:August 13, 1987 - v1
Checksum:i20341187BE4412ED
GO
Isoform Gnas-2 (identifier: P63095-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLL → MGDSVQILLVFMDK

Show »
Length:362
Mass (Da):41,818
Checksum:i0642A708B3DFCA15
GO
Isoform Gnas-3 (identifier: P63095-3) [UniParc]FASTAAdd to basket
Also known as: GsaN1

The sequence of this isoform differs from the canonical sequence as follows:
     87-91: EKATK → VYYPH
     92-394: Missing.

Show »
Length:91
Mass (Da):10,184
Checksum:i77D763328B7212AD
GO
Isoform XLas-1 (identifier: Q63803-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q63803.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,144
Mass (Da):122,887
GO
Isoform Nesp55 (identifier: Q792G6-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q792G6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Shares no sequence similarity with other isoforms due to a novel first exon containing the entire reading frame spliced to shared exon 2 so that exons 2-13 make up the 3'-UTR.
Length:256
Mass (Da):29,219
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4646MGCLG…RLLLL → MGDSVQILLVFMDK in isoform Gnas-2. 1 PublicationVSP_021154Add
BLAST
Alternative sequencei87 – 915EKATK → VYYPH in isoform Gnas-3. 1 PublicationVSP_021155
Alternative sequencei92 – 394303Missing in isoform Gnas-3. 1 PublicationVSP_021156Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12673 mRNA. Translation: AAA41261.1.
M17525 mRNA. Translation: AAA40827.1.
L10326 mRNA. Translation: AAA41664.1.
AF107844 mRNA. Translation: AAD11802.1.
DQ120475 mRNA. Translation: AAZ23814.1.
DQ120476 mRNA. Translation: AAZ23815.1.
BC061967 mRNA. Translation: AAH61967.1.
PIRiA27423. RGRTA2.
A46685.
RefSeqiNP_062005.1. NM_019132.1. [P63095-1]
UniGeneiRn.127731.
Rn.31.

Genome annotation databases

GeneIDi24896.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12673 mRNA. Translation: AAA41261.1.
M17525 mRNA. Translation: AAA40827.1.
L10326 mRNA. Translation: AAA41664.1.
AF107844 mRNA. Translation: AAD11802.1.
DQ120475 mRNA. Translation: AAZ23814.1.
DQ120476 mRNA. Translation: AAZ23815.1.
BC061967 mRNA. Translation: AAH61967.1.
PIRiA27423. RGRTA2.
A46685.
RefSeqiNP_062005.1. NM_019132.1. [P63095-1]
UniGeneiRn.127731.
Rn.31.

3D structure databases

ProteinModelPortaliP63095.
SMRiP63095. Positions 9-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247005. 1 interaction.
IntActiP63095. 1 interaction.

PTM databases

iPTMnetiP63095.
PhosphoSiteiP63095.
SwissPalmiP63095.

Proteomic databases

PRIDEiP63095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24896.

Organism-specific databases

CTDi2778.
RGDi2716. Gnas.

Phylogenomic databases

HOVERGENiHBG063184.

Enzyme and pathway databases

ReactomeiR-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 2 hits.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNAS2_RAT
AccessioniPrimary (citable) accession number: P63095
Secondary accession number(s): P04894
, P08755, Q05087, Q45QM7, Q9Z1R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is produced by a bicistronic gene which also produces the ALEX protein from an overlapping reading frame.
The GNAS locus is imprinted in a complex manner, giving rise to distinct paternally, maternally and biallelically expressed proteins. The XLas isoforms are paternally derived, the Gnas isoforms are biallelically derived and the Nesp55 isoforms are maternally derived.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.