Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Gene

Gnas

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2.By similarity

Miscellaneous

This protein is produced by a bicistronic gene which also produces the ALEX protein from an overlapping reading frame.
The GNAS locus is imprinted in a complex manner, giving rise to distinct paternally, maternally and biallelically expressed proteins. The XLas isoforms are paternally derived, the Gnas isoforms are biallelically derived and the Nesp55 isoforms are maternally derived.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54MagnesiumBy similarity1
Metal bindingi204MagnesiumBy similarity1
Binding sitei366GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 55GTPBy similarity9
Nucleotide bindingi197 – 204GTPBy similarity8
Nucleotide bindingi223 – 227GTPBy similarity5
Nucleotide bindingi292 – 295GTPBy similarity4

GO - Molecular functioni

  • alkylglycerophosphoethanolamine phosphodiesterase activity Source: MGI
  • alpha-tubulin binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein beta/gamma-subunit complex binding Source: InterPro
  • G-protein beta-subunit binding Source: RGD
  • GTPase activity Source: InterPro
  • GTP binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • mu-type opioid receptor binding Source: RGD
  • protein domain specific binding Source: RGD
  • signal transducer activity Source: RGD

GO - Biological processi

  • adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
  • adenylate cyclase-activating dopamine receptor signaling pathway Source: BHF-UCL
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • calcium ion homeostasis Source: RGD
  • cellular response to catecholamine stimulus Source: BHF-UCL
  • cellular response to prostaglandin E stimulus Source: BHF-UCL
  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of renal sodium excretion Source: RGD
  • positive regulation of cAMP-mediated signaling Source: UniProtKB
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of sodium ion transport Source: RGD
  • regulation of transcription by RNA polymerase II Source: RGD

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein
G-alpha-8
Gene namesi
Name:Gnas
Synonyms:Gnas1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2716 Gnas

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi3C → A: Abolishes S-palmitoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002037252 – 394Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-palmitoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteine1 Publication1
Cross-linki300Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei352PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP63095

PTM databases

iPTMnetiP63095
PhosphoSitePlusiP63095
SwissPalmiP63095

Expressioni

Tissue specificityi

Isoform Gnas-3 is abundant in brain with intermediate levels in skeletal muscle and very low levels in other tissues.1 Publication

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (By similarity). Interacts with CRY1; the interaction may block GPCR-mediated regulation of cAMP concentrations. Interacts with ADCY6 and stimulates its adenylyl cyclase activity (By similarity). Interacts with ADCY2 and ADCY5 (By similarity). Stimulates the ADCY5 adenylyl cyclase activity (By similarity).By similarity

GO - Molecular functioni

  • alpha-tubulin binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein beta-subunit binding Source: RGD
  • mu-type opioid receptor binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi247005, 1 interactor
IntActiP63095, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliP63095
SMRiP63095
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

HOVERGENiHBG063184

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR000367 Gprotein_alpha_S
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00443 GPROTEINAS
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gnas-1 (identifier: P63095-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE
60 70 80 90 100
SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK
110 120 130 140 150
EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPNF DFPPEFYEHA
160 170 180 190 200
KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQADY VPSDQDLLRC
210 220 230 240 250
RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVAS
260 270 280 290 300
SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK
310 320 330 340 350
VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST
360 370 380 390
ASGDGRHYCY PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL
Length:394
Mass (Da):45,664
Last modified:August 13, 1987 - v1
Checksum:i20341187BE4412ED
GO
Isoform Gnas-2 (identifier: P63095-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLL → MGDSVQILLVFMDK

Show »
Length:362
Mass (Da):41,818
Checksum:i0642A708B3DFCA15
GO
Isoform Gnas-3 (identifier: P63095-3) [UniParc]FASTAAdd to basket
Also known as: GsaN1

The sequence of this isoform differs from the canonical sequence as follows:
     87-91: EKATK → VYYPH
     92-394: Missing.

Show »
Length:91
Mass (Da):10,184
Checksum:i77D763328B7212AD
GO
Isoform XLas-1 (identifier: Q63803-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q63803.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,144
Mass (Da):122,887
GO
Isoform Nesp55 (identifier: Q792G6-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q792G6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Shares no sequence similarity with other isoforms due to a novel first exon containing the entire reading frame spliced to shared exon 2 so that exons 2-13 make up the 3'-UTR.
Length:256
Mass (Da):29,219
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0211541 – 46MGCLG…RLLLL → MGDSVQILLVFMDK in isoform Gnas-2. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_02115587 – 91EKATK → VYYPH in isoform Gnas-3. 1 Publication5
Alternative sequenceiVSP_02115692 – 394Missing in isoform Gnas-3. 1 PublicationAdd BLAST303

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12673 mRNA Translation: AAA41261.1
M17525 mRNA Translation: AAA40827.1
L10326 mRNA Translation: AAA41664.1
AF107844 mRNA Translation: AAD11802.1
DQ120475 mRNA Translation: AAZ23814.1
DQ120476 mRNA Translation: AAZ23815.1
BC061967 mRNA Translation: AAH61967.1
PIRiA27423 RGRTA2
A46685
RefSeqiNP_062005.1, NM_019132.1 [P63095-1]
UniGeneiRn.127731
Rn.31

Genome annotation databases

GeneIDi24896

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGNAS2_RAT
AccessioniPrimary (citable) accession number: P63095
Secondary accession number(s): P04894
, P08755, Q05087, Q45QM7, Q9Z1R8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health