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Protein

Pleiotrophin

Gene

Ptn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. Binds to cell-surface target proteins via their chondroitin sulfate groups (By similarity). Down-regulates PTPRZ1 activity (PubMed:16814777).By similarity1 Publication

GO - Molecular functioni

  • chondroitin sulfate binding Source: Ensembl
  • chondroitin sulfate proteoglycan binding Source: RGD
  • glycosaminoglycan binding Source: RGD
  • growth factor activity Source: RGD
  • heparan sulfate binding Source: RGD
  • heparin binding Source: RGD
  • proteoglycan binding Source: RGD
  • vascular endothelial growth factor binding Source: RGD

GO - Biological processi

  • bone mineralization Source: Ensembl
  • brain development Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to platelet-derived growth factor stimulus Source: RGD
  • cellular response to UV Source: RGD
  • cellular response to vitamin D Source: RGD
  • cerebellum development Source: RGD
  • endothelial cell differentiation Source: RGD
  • estrous cycle Source: RGD
  • heart development Source: RGD
  • hindbrain development Source: RGD
  • learning Source: RGD
  • liver development Source: RGD
  • long-term synaptic potentiation Source: RGD
  • lung development Source: RGD
  • negative regulation of angiogenesis Source: RGD
  • negative regulation of cell migration Source: RGD
  • negative regulation of epithelial cell proliferation Source: RGD
  • negative regulation of glial cell proliferation Source: RGD
  • negative regulation of membrane potential Source: RGD
  • negative regulation of mesenchymal cell proliferation Source: RGD
  • negative regulation of neuromuscular junction development Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of cell-substrate adhesion Source: RGD
  • positive regulation of hepatocyte proliferation Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of skeletal muscle acetylcholine-gated channel clustering Source: RGD
  • regulation of cell shape Source: RGD
  • response to activity Source: RGD
  • response to ciliary neurotrophic factor Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to kainic acid Source: RGD
  • response to nerve growth factor Source: RGD
  • response to progesterone Source: RGD
  • retina development in camera-type eye Source: RGD
  • retinal rod cell differentiation Source: RGD
  • rod bipolar cell differentiation Source: RGD
  • spinal cord development Source: RGD
  • thalamus development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pleiotrophin
Short name:
PTN
Alternative name(s):
Heparin-binding brain mitogen
Short name:
HBBM
Heparin-binding growth factor 8
Short name:
HBGF-8
Heparin-binding growth-associated molecule
Short name:
HB-GAM
Heparin-binding neutrophic factor
Short name:
HBNF
Osteoblast-specific factor 1
Short name:
OSF-1
Gene namesi
Name:Ptn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3444. Ptn.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

  • basement membrane Source: RGD
  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: Ensembl
  • extracellular region Source: RGD
  • extracellular space Source: Ensembl
  • membrane Source: RGD
  • neuromuscular junction Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 321 PublicationAdd BLAST32
ChainiPRO_000002466133 – 168PleiotrophinAdd BLAST136

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 76By similarity
Disulfide bondi55 ↔ 85By similarity
Disulfide bondi62 ↔ 89By similarity
Disulfide bondi99 ↔ 131By similarity
Disulfide bondi109 ↔ 141By similarity

Post-translational modificationi

Phosphorylated by NEK6.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP63090.
PRIDEiP63090.

Expressioni

Tissue specificityi

Expressed in brain.

Developmental stagei

Expressed at low levels in the brain in early embryonic stages. Levels increase to a maximum before or just after birth, and are lower in adult brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000011946.
GenevisibleiP63090. RN.

Interactioni

Subunit structurei

Interacts with ALK and NEK6 (By similarity). Interacts with PTPRZ1 (probably via chondroitin sulfate groups) (PubMed:16814777).By similarity1 Publication

GO - Molecular functioni

  • growth factor activity Source: RGD
  • vascular endothelial growth factor binding Source: RGD

Protein-protein interaction databases

BioGridi247030. 1 interactor.
STRINGi10116.ENSRNOP00000016088.

Structurei

3D structure databases

ProteinModelPortaliP63090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 99Chondroitin sulfate bindingBy similarity8
Regioni123 – 131Chondroitin sulfate bindingBy similarity9
Regioni147 – 168Chondroitin sulfate A bindingBy similarityAdd BLAST22

Sequence similaritiesi

Belongs to the pleiotrophin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWNA. Eukaryota.
ENOG4111ZWP. LUCA.
GeneTreeiENSGT00390000007640.
HOGENOMiHOG000231473.
HOVERGENiHBG008317.
InParanoidiP63090.
KOiK16642.
OMAiRMQSQQY.
OrthoDBiEOG091G0VCN.
PhylomeDBiP63090.
TreeFamiTF332376.

Family and domain databases

Gene3Di2.20.60.10. 1 hit.
2.30.90.10. 1 hit.
InterProiIPR000762. Midkine_heparin-bd_GF.
IPR020090. PTN/MK_C_dom.
IPR020091. PTN/MK_diS.
IPR020089. PTN/MK_N_dom.
IPR020092. PTN_MK_heparin-bd_GF_CS.
[Graphical view]
PANTHERiPTHR13850. PTHR13850. 1 hit.
PfamiPF01091. PTN_MK_C. 1 hit.
PF05196. PTN_MK_N. 1 hit.
[Graphical view]
PRINTSiPR00269. PTNMIDKINE.
ProDomiPD005592. PTN_MK_hepar_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00193. PTN. 1 hit.
[Graphical view]
SUPFAMiSSF57288. SSF57288. 2 hits.
PROSITEiPS00619. PTN_MK_1. 1 hit.
PS00620. PTN_MK_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQQYQQQR RKFAAAFLAL IFILAAVDTA EAGKKEKPEK KVKKSDCGEW
60 70 80 90 100
QWSVCVPTSG DCGLGTREGT RTGAECKQTM KTQRCKIPCN WKKQFGAECK
110 120 130 140 150
YQFQAWGECD LNTALKTRTG SLKRALHNAD CQKTVTISKP CGKLTKPKPQ
160
AESKKKKKEG KKQEKMLD
Length:168
Mass (Da):18,869
Last modified:September 13, 2004 - v1
Checksum:i2127DA167D2DD33D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55601 mRNA. Translation: AAA41310.1.
M68916 mRNA. Translation: AAA41311.1.
BC062013 mRNA. Translation: AAH62013.1.
PIRiB37780.
RefSeqiNP_058762.1. NM_017066.2.
UniGeneiRn.1653.

Genome annotation databases

EnsembliENSRNOT00000016088; ENSRNOP00000016088; ENSRNOG00000011946.
GeneIDi24924.
KEGGirno:24924.
UCSCiRGD:3444. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55601 mRNA. Translation: AAA41310.1.
M68916 mRNA. Translation: AAA41311.1.
BC062013 mRNA. Translation: AAH62013.1.
PIRiB37780.
RefSeqiNP_058762.1. NM_017066.2.
UniGeneiRn.1653.

3D structure databases

ProteinModelPortaliP63090.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247030. 1 interactor.
STRINGi10116.ENSRNOP00000016088.

Proteomic databases

PaxDbiP63090.
PRIDEiP63090.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016088; ENSRNOP00000016088; ENSRNOG00000011946.
GeneIDi24924.
KEGGirno:24924.
UCSCiRGD:3444. rat.

Organism-specific databases

CTDi5764.
RGDi3444. Ptn.

Phylogenomic databases

eggNOGiENOG410IWNA. Eukaryota.
ENOG4111ZWP. LUCA.
GeneTreeiENSGT00390000007640.
HOGENOMiHOG000231473.
HOVERGENiHBG008317.
InParanoidiP63090.
KOiK16642.
OMAiRMQSQQY.
OrthoDBiEOG091G0VCN.
PhylomeDBiP63090.
TreeFamiTF332376.

Miscellaneous databases

PROiP63090.

Gene expression databases

BgeeiENSRNOG00000011946.
GenevisibleiP63090. RN.

Family and domain databases

Gene3Di2.20.60.10. 1 hit.
2.30.90.10. 1 hit.
InterProiIPR000762. Midkine_heparin-bd_GF.
IPR020090. PTN/MK_C_dom.
IPR020091. PTN/MK_diS.
IPR020089. PTN/MK_N_dom.
IPR020092. PTN_MK_heparin-bd_GF_CS.
[Graphical view]
PANTHERiPTHR13850. PTHR13850. 1 hit.
PfamiPF01091. PTN_MK_C. 1 hit.
PF05196. PTN_MK_N. 1 hit.
[Graphical view]
PRINTSiPR00269. PTNMIDKINE.
ProDomiPD005592. PTN_MK_hepar_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00193. PTN. 1 hit.
[Graphical view]
SUPFAMiSSF57288. SSF57288. 2 hits.
PROSITEiPS00619. PTN_MK_1. 1 hit.
PS00620. PTN_MK_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTN_RAT
AccessioniPrimary (citable) accession number: P63090
Secondary accession number(s): P20935
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.