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Protein

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

Gene

Ppp1cc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E.2 Publications
Isoform 2: Required for normal male fertility.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inactivated by binding to URI1.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Manganese 1By similarity1
Metal bindingi66Manganese 1By similarity1
Metal bindingi92Manganese 1By similarity1
Metal bindingi92Manganese 2By similarity1
Metal bindingi124Manganese 2By similarity1
Active sitei125Proton donorBy similarity1
Metal bindingi173Manganese 2By similarity1
Metal bindingi248Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • circadian regulation of gene expression Source: UniProtKB
  • entrainment of circadian clock by photoperiod Source: UniProtKB
  • glycogen metabolic process Source: UniProtKB-KW
  • neuron differentiation Source: MGI
  • protein dephosphorylation Source: UniProtKB
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of nucleocytoplasmic transport Source: MGI

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processBiological rhythms, Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (EC:3.1.3.16)
Short name:
PP-1G
Alternative name(s):
Protein phosphatase 1C catalytic subunit
Gene namesi
Name:Ppp1cc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:104872 Ppp1cc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Deficient mice exhibit male infertility due to severely impaired spermiogenesis beginning at the round spermatid stage. Females are fertile.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000587882 – 323Serine/threonine-protein phosphatase PP1-gamma catalytic subunitAdd BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei307PhosphothreonineBy similarity1
Modified residuei311PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated by NEK2.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63087
PaxDbiP63087
PeptideAtlasiP63087
PRIDEiP63087

PTM databases

iPTMnetiP63087
PhosphoSitePlusiP63087
SwissPalmiP63087

Expressioni

Tissue specificityi

Isoform 1 is widely expressed (PubMed:9339378). Isoform 2 is highly enriched in testis, mainly restricted to meiotic and postmeiotic germ cells (PubMed:9339378).1 Publication

Gene expression databases

BgeeiENSMUSG00000004455
ExpressionAtlasiP63087 baseline and differential
GenevisibleiP63087 MM

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen. Interacts with PPP1R3B, PPP1R7 and CDCA2 (By similarity). Isoform 2 interacts with SPZ1. This interaction can prevent SPZ1 binding to the E-box and inhibits PPP1CC activity. PPP1R15A and PPP1R15B mediate binding to EIF2S1. Part of a complex containing PPP1R15B, PP1 and NCK1/2. Interacts with IKFZ1; the interaction targets PPP1CC to pericentromeric heterochromatin, dephosphorylates IKAROS, stabilizes it and prevents it from degradation. Interacts with NOM1 and PPP1R8. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1R8. Interacts with NEK2. Interacts with NEK2. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner (By similarity). Isoform 2 interacts with PPP1R42; the interaction is direct. Interacts with FOXP3 (By similarity). Isoform 2 interacts with TMEM225 (via RVxF motif) (PubMed:25605614). Isoform 2, but not isoform 1, interacts with Sh3glb1 testis-specific isoform 3; this interaction leads to the inhibition of phosphatase activity (PubMed:17381077). Interacts with MKI67 (By similarity). Interacts with RRP1B; this targets PPP1CC to the nucleolus (By similarity).By similarity7 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202337, 38 interactors
IntActiP63087, 27 interactors
MINTiP63087
STRINGi10090.ENSMUSP00000099587

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 17Combined sources9
Turni18 – 21Combined sources4
Helixi32 – 48Combined sources17
Beta strandi51 – 55Combined sources5
Beta strandi57 – 62Combined sources6
Helixi69 – 79Combined sources11
Beta strandi87 – 89Combined sources3
Beta strandi94 – 98Combined sources5
Helixi100 – 113Combined sources14
Turni115 – 117Combined sources3
Beta strandi118 – 120Combined sources3
Helixi128 – 131Combined sources4
Helixi136 – 143Combined sources8
Helixi146 – 156Combined sources11
Beta strandi162 – 165Combined sources4
Turni166 – 168Combined sources3
Beta strandi169 – 174Combined sources6
Helixi184 – 187Combined sources4
Beta strandi197 – 199Combined sources3
Helixi200 – 206Combined sources7
Beta strandi214 – 218Combined sources5
Beta strandi222 – 227Combined sources6
Helixi229 – 239Combined sources11
Beta strandi242 – 246Combined sources5
Beta strandi254 – 258Combined sources5
Turni259 – 262Combined sources4
Beta strandi263 – 267Combined sources5
Helixi272 – 274Combined sources3
Beta strandi280 – 285Combined sources6
Beta strandi290 – 298Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V0UX-ray7.88D/F/H/J/N1-323[»]
4V0VX-ray1.61A/C7-300[»]
4V0WX-ray1.55A/C7-300[»]
4V0XX-ray1.85A7-300[»]
ProteinModelPortaliP63087
SMRiP63087
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN85 Eukaryota
ENOG410XPVF LUCA
GeneTreeiENSGT00530000062911
HOGENOMiHOG000172697
HOVERGENiHBG000216
InParanoidiP63087
KOiK06269
OMAiQRGYEFF
OrthoDBiEOG091G0EKF
PhylomeDBiP63087
TreeFamiTF354243

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR037981 PPP1CC
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PANTHERiPTHR11668:SF204 PTHR11668:SF204, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P63087-1) [UniParc]FASTAAdd to basket
Also known as: Gamma-1, Pppcc1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADIDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP
60 70 80 90 100
ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS
110 120 130 140 150
LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK
160 170 180 190 200
TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL
210 220 230 240 250
LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV
260 270 280 290 300
VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE
310 320
KKKPNATRPV TPPRGMITKQ AKK
Length:323
Mass (Da):36,984
Last modified:September 13, 2004 - v1
Checksum:i4E28412C16898615
GO
Isoform 2 (identifier: P63087-2) [UniParc]FASTAAdd to basket
Also known as: Gamma-2, Pppcc2

The sequence of this isoform differs from the canonical sequence as follows:
     315-323: GMITKQAKK → VGSGLNPSIQKASNYRNNTVLYE

Show »
Length:337
Mass (Da):38,504
Checksum:iE6F271155E0E67D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316 – 317MI → IV in AAC53383 (PubMed:9339378).Curated2
Sequence conflicti316 – 317MI → IV in AAC53384 (PubMed:9339378).Curated2
Sequence conflicti316 – 317MI → IV in BAA19729 (Ref. 3) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005095315 – 323GMITKQAKK → VGSGLNPSIQKASNYRNNTV LYE in isoform 2. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27071 mRNA Translation: AAA37526.1
U53456 mRNA Translation: AAC53383.1
U53276
, U53271, U53272, U53273, U53275 Genomic DNA Translation: AAC53384.1
U53276
, U53271, U53272, U53273, U53275 Genomic DNA Translation: AAC53385.1
D85137 mRNA Translation: BAA19729.1
BC010613 mRNA Translation: AAH10613.1
BC021646 mRNA Translation: AAH21646.1
BC085496 mRNA Translation: AAH85496.1
CCDSiCCDS19643.1 [P63087-1]
PIRiC32550
RefSeqiNP_038664.2, NM_013636.3 [P63087-1]
XP_006508478.1, XM_006508415.3 [P63087-2]
XP_006530263.1, XM_006530200.2 [P63087-2]
UniGeneiMm.280784
Mm.334198

Genome annotation databases

EnsembliENSMUST00000086294; ENSMUSP00000083474; ENSMUSG00000004455 [P63087-2]
ENSMUST00000102528; ENSMUSP00000099587; ENSMUSG00000004455 [P63087-1]
GeneIDi19047
434233
KEGGimmu:19047
mmu:434233
UCSCiuc008zks.1 mouse [P63087-1]
uc008zkt.1 mouse [P63087-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPP1G_MOUSE
AccessioniPrimary (citable) accession number: P63087
Secondary accession number(s): O09186
, O09189, P37139, Q64679
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health