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Reviewed, UniProtKB/Swiss-Prot P63087 (PP1G_MOUSE)

Last modified July 7, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
      Short name=PP-1G
    EC=3.1.3.16
Alternative name(s):
    Protein phosphatase 1C catalytic subunit
Gene names
Name: Ppp1cc
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II By similarity.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with PPP1R3B, PPP1R7 and CDCA2 By similarity. Isoform gamma-2 interacts with SPZ1. This interaction can prevent SPZ1 binding to the E-box and inhibits PPP1CC activity. PPP1R15A and PPP1R15B mediate binding to EIF2S1. Part of a complex containing PPP1R15B, PP1 and NCK1/2.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Colocalizes with SPZ1 in the nucleus.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Gamma-1 (identifier: P63087-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Gamma-2 (identifier: P63087-2)

The sequence of this isoform differs from the canonical sequence as follows:
     315-323: GMITKQAKK → VGSGLNPSIQKASNYRNNTVLYE

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 323322Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
PRO_0000058788

Sites

Active site1251Proton donor By similarity
Metal binding641Iron By similarity
Metal binding661Iron By similarity
Metal binding921Iron By similarity
Metal binding921Manganese By similarity
Metal binding1241Manganese By similarity
Metal binding1731Manganese By similarity
Metal binding2481Manganese By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3071Phosphothreonine By similarity

Natural variations

Alternative sequence315 – 3239GMITKQAKK → VGSGLNPSIQKASNYRNNTV LYE in isoform Gamma-2.
VSP_005095

Experimental info

Sequence conflict316 – 3172MI → IV in AAC53383. Ref.2
Sequence conflict316 – 3172MI → IV in AAC53384. Ref.2
Sequence conflict316 – 3172MI → IV in BAA19729. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform Gamma-1 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 4E28412C16898615

FASTA32336,984
        10         20         30         40         50         60 
MADIDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK 

        70         80         90        100        110        120 
ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL 

       130        140        150        160        170        180 
LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL 

       190        200        210        220        230        240 
QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD 

       250        260        270        280        290        300 
LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE 

       310        320 
KKKPNATRPV TPPRGMITKQ AKK

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Isoform Gamma-2.

Checksum: E6F271155E0E67D7
Show »

FASTA33738,504

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References

« Hide 'large scale' references
[1]"The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases."
Ohkura H., Kinoshita N., Miyatani S., Toda T., Yanagida M.
Cell 57:997-1007(1989) [PubMed: 2544298] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA-2).
Tissue: Brain.
[2]"Genomic organization and functional analysis of the murine protein phosphatase 1c gamma (Ppp1cc) gene."
Okano K., Heng H., Trevisanato S., Tyers M., Varmuza S.
Genomics 45:211-215(1997) [PubMed: 9339378] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS GAMMA-1 AND GAMMA-2), ALTERNATIVE SPLICING.
Strain: 129 and CD-1.
Tissue: Embryo.
[3]"Tertially complex among Pim-1 kinase, protein phosphatase 1 gamma, and PAP-1."
Harada Y., Sato E., Izumi F., Imamura Y., Ariga H., Iguchi-Ariga S.
Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA-1).
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GAMMA-1).
Strain: C57BL/6, C57BL/6J and NOD.
Tissue: Brain and Thymus.
[5]Lubec G., Klug S.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 61-74 AND 114-122, MASS SPECTROMETRY.
Tissue: Hippocampus.
[6]"Inhibition of a constitutive translation initiation factor 2alpha phosphatase, CReP, promotes survival of stressed cells."
Jousse C., Oyadomari S., Novoa I., Lu P., Zhang Y., Harding H.P., Ron D.
J. Cell Biol. 163:767-775(2003) [PubMed: 14638860] [Abstract]
Cited for: INTERACTION WITH PPP1R15B.
[7]"Identification of the spermatogenic zip protein Spz1 as a putative protein phosphatase-1 (PP1) regulatory protein that specifically binds the PP1cgamma2 splice variant in mouse testis."
Hrabchak C., Varmuza S.
J. Biol. Chem. 279:37079-37086(2004) [PubMed: 15226296] [Abstract]
Cited for: INTERACTION WITH SPZ1.
[8]"Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress."
Latreille M., Larose L.
J. Biol. Chem. 281:26633-26644(2006) [PubMed: 16835242] [Abstract]
Cited for: IDENTIFICATION IN COMPLEX WITH PPP1R15B AND NCK1.
+Additional computationally mapped references.

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Web resources

Protein Spotlight

The things we forget - Issue 32 of March 2003

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Cross-references

Sequence databases

M27071 mRNA. Translation: AAA37526.1.
U53456 mRNA. Translation: AAC53383.1.
U53276 expand/collapse EMBL AC list , U53271, U53272, U53273, U53275 Genomic DNA. Translation: AAC53384.1.
U53276 expand/collapse EMBL AC list , U53271, U53272, U53273, U53275 Genomic DNA. Translation: AAC53385.1.
D85137 mRNA. Translation: BAA19729.1.
BC010613 mRNA. Translation: AAH10613.1.
BC021646 mRNA. Translation: AAH21646.1.
BC085496 mRNA. Translation: AAH85496.1.
IPIIPI00123862.
IPI00227773.
PIRC32550.
RefSeqNP_038664.2.
UniGeneMm.280784
Mm.474554

3D structure databases

HSSPHSSP built from PDB template 1JK7 based on UniProtKB P36873.
SMRP63087. Positions 6-299.
ModBaseSearch...

Protein-protein interaction databases

IntActP63087. 22 interactions.

Proteomic databases

PRIDEP63087.

Genome annotation databases

EnsemblENSMUSG00000004455. Mus musculus. [Contig view]
GeneID19047.
KEGGmmu:19047.

Organism-specific databases

MGIMGI:104872. Ppp1cc.

Phylogenomic databases

HOGENOMP63087.
HOVERGENP63087.
OMAP63087. MITKQAK.

Enzyme and pathway databases

BRENDA3.1.3.16. 244.

Gene expression databases

ArrayExpressP63087.
BgeeP63087.
GermOnlineENSMUSG00000044341. Mus musculus.

Family and domain databases

InterProIPR004843. M-pesterase.
IPR006186. T_phtase_apaH.
[Graphical view]
PANTHERPTHR11668. T_phtase_apaH. 1 hit.
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
ProDomPD000252. T_phtase_apaH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio295513.
SOURCESearch...

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Entry information

Entry namePP1G_MOUSE
AccessionPrimary (citable) accession number: P63087
Secondary accession number(s): O09186 expand/collapse secondary AC list , O09189, P37139, Q64679
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: July 7, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents