Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

Gene

Ppp1cc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inactivated by binding to URI1.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Manganese 1By similarity
Metal bindingi66 – 661Manganese 1By similarity
Metal bindingi92 – 921Manganese 1By similarity
Metal bindingi92 – 921Manganese 2By similarity
Metal bindingi124 – 1241Manganese 2By similarity
Active sitei125 – 1251Proton donorBy similarity
Metal bindingi173 – 1731Manganese 2By similarity
Metal bindingi248 – 2481Manganese 2By similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphatase activity Source: UniProtKB
  • poly(A) RNA binding Source: Ensembl
  • protein N-terminus binding Source: MGI
  • protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • circadian regulation of gene expression Source: UniProtKB
  • entrainment of circadian clock by photoperiod Source: UniProtKB
  • glycogen metabolic process Source: UniProtKB-KW
  • neuron differentiation Source: MGI
  • protein dephosphorylation Source: UniProtKB
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of nucleocytoplasmic transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Biological rhythms, Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (EC:3.1.3.16)
Short name:
PP-1G
Alternative name(s):
Protein phosphatase 1C catalytic subunit
Gene namesi
Name:Ppp1cc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:104872. Ppp1cc.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Cleavage furrow By similarity
  • Nucleusnucleolus By similarity
  • Nucleusnucleoplasm By similarity
  • Chromosomecentromerekinetochore By similarity
  • Nucleus speckle By similarity
  • Midbody By similarity
  • Mitochondrion By similarity

  • Note: Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles. Shows a dynamic targeting to specific sites throughout the cell cycle. Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis. Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase. Also accumulates at the cleavage furrow and midbody by telophase. Colocalizes with SPZ1 in the nucleus. Colocalizes with URI1 at mitochondrion (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 323322Serine/threonine-protein phosphatase PP1-gamma catalytic subunitPRO_0000058788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei307 – 3071PhosphothreonineBy similarity
Modified residuei311 – 3111PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated by NEK2.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63087.
MaxQBiP63087.
PaxDbiP63087.
PeptideAtlasiP63087.
PRIDEiP63087.

PTM databases

iPTMnetiP63087.
PhosphoSiteiP63087.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004455.
ExpressionAtlasiP63087. baseline.
GenevisibleiP63087. MM.

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with PPP1R3B, PPP1R7 and CDCA2 (By similarity). Isoform gamma-2 interacts with SPZ1. This interaction can prevent SPZ1 binding to the E-box and inhibits PPP1CC activity. PPP1R15A and PPP1R15B mediate binding to EIF2S1. Part of a complex containing PPP1R15B, PP1 and NCK1/2. Interacts with IKFZ1; the interaction targets PPP1CC to pericentromeric heterochromatin, dephosphorylates IKAROS, stabilizes it and prevents it from degradation. Interacts with NOM1 and PPP1R8. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1R8. Interacts with NEK2. Interacts with NEK2. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner (By similarity). Isoform 2 interacts with PPP1R42; the interaction is direct. Interacts with FOXP3 (By similarity). Isoform 2 interacts with TMEM225 (via RVxF motif) (PubMed:25605614). Isoform Gamma-2, but not isoform Gamma-1, interacts with Sh3glb1 testis-specific isoform 3; this interaction leads to the inhibition of phosphatase activity (PubMed:17381077).By similarity7 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202337. 34 interactions.
IntActiP63087. 23 interactions.
MINTiMINT-4591330.
STRINGi10090.ENSMUSP00000099587.

Structurei

Secondary structure

1
323
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 179Combined sources
Turni18 – 214Combined sources
Helixi32 – 4817Combined sources
Beta strandi51 – 555Combined sources
Beta strandi57 – 626Combined sources
Helixi69 – 7911Combined sources
Beta strandi87 – 893Combined sources
Beta strandi94 – 985Combined sources
Helixi100 – 11314Combined sources
Turni115 – 1173Combined sources
Beta strandi118 – 1203Combined sources
Helixi128 – 1314Combined sources
Helixi136 – 1438Combined sources
Helixi146 – 15611Combined sources
Beta strandi162 – 1654Combined sources
Turni166 – 1683Combined sources
Beta strandi169 – 1746Combined sources
Helixi184 – 1874Combined sources
Beta strandi197 – 1993Combined sources
Helixi200 – 2067Combined sources
Beta strandi214 – 2185Combined sources
Beta strandi222 – 2276Combined sources
Helixi229 – 23911Combined sources
Beta strandi242 – 2465Combined sources
Beta strandi254 – 2585Combined sources
Turni259 – 2624Combined sources
Beta strandi263 – 2675Combined sources
Helixi272 – 2743Combined sources
Beta strandi280 – 2856Combined sources
Beta strandi290 – 2989Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V0UX-ray7.88D/F/H/J/N1-323[»]
4V0VX-ray1.61A/C7-300[»]
4V0WX-ray1.55A/C7-300[»]
4V0XX-ray1.85A7-300[»]
ProteinModelPortaliP63087.
SMRiP63087. Positions 7-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN85. Eukaryota.
ENOG410XPVF. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
HOVERGENiHBG000216.
InParanoidiP63087.
KOiK06269.
OMAiIQRLLEX.
OrthoDBiEOG091G0EKF.
PhylomeDBiP63087.
TreeFamiTF354243.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma-1 (identifier: P63087-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADIDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP
60 70 80 90 100
ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS
110 120 130 140 150
LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK
160 170 180 190 200
TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL
210 220 230 240 250
LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV
260 270 280 290 300
VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE
310 320
KKKPNATRPV TPPRGMITKQ AKK
Length:323
Mass (Da):36,984
Last modified:September 13, 2004 - v1
Checksum:i4E28412C16898615
GO
Isoform Gamma-2 (identifier: P63087-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     315-323: GMITKQAKK → VGSGLNPSIQKASNYRNNTVLYE

Show »
Length:337
Mass (Da):38,504
Checksum:iE6F271155E0E67D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti316 – 3172MI → IV in AAC53383 (PubMed:9339378).Curated
Sequence conflicti316 – 3172MI → IV in AAC53384 (PubMed:9339378).Curated
Sequence conflicti316 – 3172MI → IV in BAA19729 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei315 – 3239GMITKQAKK → VGSGLNPSIQKASNYRNNTV LYE in isoform Gamma-2. 2 PublicationsVSP_005095

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27071 mRNA. Translation: AAA37526.1.
U53456 mRNA. Translation: AAC53383.1.
U53276
, U53271, U53272, U53273, U53275 Genomic DNA. Translation: AAC53384.1.
U53276
, U53271, U53272, U53273, U53275 Genomic DNA. Translation: AAC53385.1.
D85137 mRNA. Translation: BAA19729.1.
BC010613 mRNA. Translation: AAH10613.1.
BC021646 mRNA. Translation: AAH21646.1.
BC085496 mRNA. Translation: AAH85496.1.
CCDSiCCDS19643.1. [P63087-1]
PIRiC32550.
RefSeqiNP_038664.2. NM_013636.3. [P63087-1]
XP_006508478.1. XM_006508415.2. [P63087-2]
XP_006530263.1. XM_006530200.1. [P63087-2]
XP_006544330.1. XM_006544267.2. [P63087-2]
UniGeneiMm.280784.
Mm.334198.

Genome annotation databases

EnsembliENSMUST00000086294; ENSMUSP00000083474; ENSMUSG00000004455. [P63087-2]
ENSMUST00000102528; ENSMUSP00000099587; ENSMUSG00000004455. [P63087-1]
GeneIDi19047.
434233.
KEGGimmu:19047.
mmu:434233.
UCSCiuc008zks.1. mouse. [P63087-1]
uc008zkt.1. mouse. [P63087-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

The things we forget - Issue 32 of March 2003

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27071 mRNA. Translation: AAA37526.1.
U53456 mRNA. Translation: AAC53383.1.
U53276
, U53271, U53272, U53273, U53275 Genomic DNA. Translation: AAC53384.1.
U53276
, U53271, U53272, U53273, U53275 Genomic DNA. Translation: AAC53385.1.
D85137 mRNA. Translation: BAA19729.1.
BC010613 mRNA. Translation: AAH10613.1.
BC021646 mRNA. Translation: AAH21646.1.
BC085496 mRNA. Translation: AAH85496.1.
CCDSiCCDS19643.1. [P63087-1]
PIRiC32550.
RefSeqiNP_038664.2. NM_013636.3. [P63087-1]
XP_006508478.1. XM_006508415.2. [P63087-2]
XP_006530263.1. XM_006530200.1. [P63087-2]
XP_006544330.1. XM_006544267.2. [P63087-2]
UniGeneiMm.280784.
Mm.334198.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V0UX-ray7.88D/F/H/J/N1-323[»]
4V0VX-ray1.61A/C7-300[»]
4V0WX-ray1.55A/C7-300[»]
4V0XX-ray1.85A7-300[»]
ProteinModelPortaliP63087.
SMRiP63087. Positions 7-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202337. 34 interactions.
IntActiP63087. 23 interactions.
MINTiMINT-4591330.
STRINGi10090.ENSMUSP00000099587.

PTM databases

iPTMnetiP63087.
PhosphoSiteiP63087.

Proteomic databases

EPDiP63087.
MaxQBiP63087.
PaxDbiP63087.
PeptideAtlasiP63087.
PRIDEiP63087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086294; ENSMUSP00000083474; ENSMUSG00000004455. [P63087-2]
ENSMUST00000102528; ENSMUSP00000099587; ENSMUSG00000004455. [P63087-1]
GeneIDi19047.
434233.
KEGGimmu:19047.
mmu:434233.
UCSCiuc008zks.1. mouse. [P63087-1]
uc008zkt.1. mouse. [P63087-2]

Organism-specific databases

CTDi5501.
MGIiMGI:104872. Ppp1cc.

Phylogenomic databases

eggNOGiENOG410IN85. Eukaryota.
ENOG410XPVF. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
HOVERGENiHBG000216.
InParanoidiP63087.
KOiK06269.
OMAiIQRLLEX.
OrthoDBiEOG091G0EKF.
PhylomeDBiP63087.
TreeFamiTF354243.

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Miscellaneous databases

ChiTaRSiPpp1cc. mouse.
PROiP63087.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004455.
ExpressionAtlasiP63087. baseline.
GenevisibleiP63087. MM.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP1G_MOUSE
AccessioniPrimary (citable) accession number: P63087
Secondary accession number(s): O09186
, O09189, P37139, Q64679
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.