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Protein

Gamma-aminobutyric acid receptor subunit beta-3

Gene

Gabrb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251AgonistBy similarity

GO - Molecular functioni

  • GABA-A receptor activity Source: UniProtKB
  • GABA-gated chloride ion channel activity Source: UniProtKB

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chloride transmembrane transport Source: UniProtKB
  • cochlea development Source: DFLAT
  • gamma-aminobutyric acid signaling pathway Source: MGI
  • inhibitory postsynaptic potential Source: CACAO
  • inner ear receptor cell development Source: DFLAT
  • innervation Source: DFLAT
  • negative regulation of neuron apoptotic process Source: DFLAT
  • negative regulation of neuron death Source: DFLAT
  • neuron development Source: DFLAT
  • palate development Source: UniProtKB
  • regulation of neuron apoptotic process Source: DFLAT
  • sensory perception of sound Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-3
Alternative name(s):
GABA(A) receptor subunit beta-3
Gene namesi
Name:Gabrb3
Synonyms:Gabrb-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95621. Gabrb3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 245220ExtracellularBy similarityAdd
BLAST
Transmembranei246 – 26722HelicalBy similarityAdd
BLAST
Topological domaini268 – 2703CytoplasmicBy similarity
Transmembranei271 – 29323HelicalBy similarityAdd
BLAST
Topological domaini294 – 30411ExtracellularBy similarityAdd
BLAST
Transmembranei305 – 32723HelicalBy similarityAdd
BLAST
Topological domaini328 – 450123CytoplasmicBy similarityAdd
BLAST
Transmembranei451 – 47222HelicalBy similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • GABA-A receptor complex Source: UniProtKB
  • inhibitory synapse Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Important perinatal lethality. About 57% of the pups have a cleft palate. About 90% of the pups die within 24 hours after birth, including 30% of those that do not have a cleft palate. Surviving mice are runted until weaning, but attain normal body weight in adulthood. They are hyperactive and display behavorial abnormalities. They do not display jerky gait, but have difficulty with swimming, walking on grids, and avoiding to fall from rotarods. Mutant mice are fertile, but females do not display normal nurturing behavior. Mutant mice have abnormal electroencephalograms (EEGs) and tend to suffer from seizures. Brains from mutant mice show strongly reduced numbers of GABA and benzodiazepine receptors. Neurons from dorsal root ganglion show a decrease of about 80% of GABA-induced chloride currents. In addition, mutant mice have an average lifespan of about 18 weeks, instead of the expected 127 weeks (PubMed:9108119). Mutant mice display a lowered threshold to nociception and are abnormally sensitive to touch and heat stimuli. GABA receptor agonists have decreased antinociceptive effects in mutant mice (PubMed:10670447).2 Publications

Chemistry

ChEMBLiCHEMBL2094133.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 473448Gamma-aminobutyric acid receptor subunit beta-3PRO_0000000463Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence analysis
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP63080.
PaxDbiP63080.
PRIDEiP63080.

PTM databases

iPTMnetiP63080.

Expressioni

Gene expression databases

BgeeiP63080.
ExpressionAtlasiP63080. baseline and differential.
GenevisibleiP63080. MM.

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Can form functional homopentamers (in vitro). Binds UBQLN1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038051.

Structurei

3D structure databases

ProteinModelPortaliP63080.
SMRiP63080. Positions 26-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 1223Agonist bindingBy similarity
Regioni180 – 1823Agonist bindingBy similarity
Regioni290 – 31122Allosteric effector bindingBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63080.
KOiK05181.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGFAGGRLF GIFSAPVLVA VVCCAQSVND PGNMSFVKET VDKLLKGYDI
60 70 80 90 100
RLRPDFGGPP VCVGMNIDIA SIDMVSEVNM DYTLTMYFQQ YWRDKRLAYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWRGGDKAV
210 220 230 240 250
TGVERIELPQ FSIVEHRLVS RNVVFATGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSILITIL SWVSFWINYD ASAARVALGI TTVLTMTTIN THLRETLPKI
310 320 330 340 350
PYVKAIDMYL MGCFVFVFLA LLEYAFVNYI FFGRGPQRQK KLAEKTAKAK
360 370 380 390 400
NDRSKSEINR VDAHGNILLA PMDVHNEMNE VAGSVGDTRN SAISFDNSGI
410 420 430 440 450
QYRKQSMPKE GHGRYMGDRS IPHKKTHLRR RSSQLKIKIP DLTDVNAIDR
460 470
WSRIVFPFTF SLFNLVYWLY YVN
Length:473
Mass (Da):54,166
Last modified:September 13, 2004 - v1
Checksum:i6A5ABD8A9143FE45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14420 mRNA. Translation: AAB60502.1.
AK039070 mRNA. Translation: BAC30230.1.
AK147247 mRNA. Translation: BAE27794.1.
CCDSiCCDS21324.1.
PIRiS53532.
RefSeqiNP_032097.1. NM_008071.3.
UniGeneiMm.8004.

Genome annotation databases

EnsembliENSMUST00000039697; ENSMUSP00000038051; ENSMUSG00000033676.
GeneIDi14402.
KEGGimmu:14402.
UCSCiuc009hee.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14420 mRNA. Translation: AAB60502.1.
AK039070 mRNA. Translation: BAC30230.1.
AK147247 mRNA. Translation: BAE27794.1.
CCDSiCCDS21324.1.
PIRiS53532.
RefSeqiNP_032097.1. NM_008071.3.
UniGeneiMm.8004.

3D structure databases

ProteinModelPortaliP63080.
SMRiP63080. Positions 26-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038051.

Chemistry

ChEMBLiCHEMBL2094133.

PTM databases

iPTMnetiP63080.

Proteomic databases

MaxQBiP63080.
PaxDbiP63080.
PRIDEiP63080.

Protocols and materials databases

DNASUi14402.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039697; ENSMUSP00000038051; ENSMUSG00000033676.
GeneIDi14402.
KEGGimmu:14402.
UCSCiuc009hee.1. mouse.

Organism-specific databases

CTDi2562.
MGIiMGI:95621. Gabrb3.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63080.
KOiK05181.

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Miscellaneous databases

ChiTaRSiGabrb3. mouse.
PROiP63080.
SOURCEiSearch...

Gene expression databases

BgeeiP63080.
ExpressionAtlasiP63080. baseline and differential.
GenevisibleiP63080. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GABAA receptor beta 1, beta 2, and beta 3 subunits: comparisons in DBA/2J and C57BL/6J mice."
    Kamatchi G.L., Kofuji P., Wang J.B., Fernando J.C., Liu Z., Mathura J.R., Burt D.R.
    Biochim. Biophys. Acta 1261:134-142(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: DBA/2J.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hypothalamus.
  3. Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. "Sensory thresholds and the antinociceptive effects of GABA receptor agonists in mice lacking the beta3 subunit of the GABA(A) receptor."
    Ugarte S.D., Homanics G.E., Firestone L.L., Hammond D.L.
    Neuroscience 95:795-806(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiGBRB3_MOUSE
AccessioniPrimary (citable) accession number: P63080
Secondary accession number(s): P15433, Q3UHR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.