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Protein

Gamma-aminobutyric acid receptor subunit beta-3

Gene

Gabrb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225AgonistBy similarity1

GO - Molecular functioni

  • GABA-A receptor activity Source: UniProtKB
  • GABA-gated chloride ion channel activity Source: UniProtKB
  • identical protein binding Source: MGI

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chloride transmembrane transport Source: UniProtKB
  • cochlea development Source: DFLAT
  • gamma-aminobutyric acid signaling pathway Source: UniProtKB
  • inhibitory postsynaptic potential Source: CACAO
  • inner ear receptor cell development Source: DFLAT
  • innervation Source: DFLAT
  • negative regulation of neuron apoptotic process Source: DFLAT
  • negative regulation of neuron death Source: DFLAT
  • neuron development Source: DFLAT
  • regulation of neuron apoptotic process Source: DFLAT
  • roof of mouth development Source: UniProtKB
  • sensory perception of sound Source: DFLAT

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-MMU-977441 GABA A receptor activation

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-3
Alternative name(s):
GABA(A) receptor subunit beta-3
Gene namesi
Name:Gabrb3
Synonyms:Gabrb-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95621 Gabrb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 245ExtracellularBy similarityAdd BLAST220
Transmembranei246 – 267HelicalBy similarityAdd BLAST22
Topological domaini268 – 270CytoplasmicBy similarity3
Transmembranei271 – 293HelicalBy similarityAdd BLAST23
Topological domaini294 – 304ExtracellularBy similarityAdd BLAST11
Transmembranei305 – 327HelicalBy similarityAdd BLAST23
Topological domaini328 – 450CytoplasmicBy similarityAdd BLAST123
Transmembranei451 – 472HelicalBy similarityAdd BLAST22

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Important perinatal lethality. About 57% of the pups have a cleft palate. About 90% of the pups die within 24 hours after birth, including 30% of those that do not have a cleft palate. Surviving mice are runted until weaning, but attain normal body weight in adulthood. They are hyperactive and display behavorial abnormalities. They do not display jerky gait, but have difficulty with swimming, walking on grids, and avoiding to fall from rotarods. Mutant mice are fertile, but females do not display normal nurturing behavior. Mutant mice have abnormal electroencephalograms (EEGs) and tend to suffer from seizures. Brains from mutant mice show strongly reduced numbers of GABA and benzodiazepine receptors. Neurons from dorsal root ganglion show a decrease of about 80% of GABA-induced chloride currents. In addition, mutant mice have an average lifespan of about 18 weeks, instead of the expected 127 weeks (PubMed:9108119). Mutant mice display a lowered threshold to nociception and are abnormally sensitive to touch and heat stimuli. GABA receptor agonists have decreased antinociceptive effects in mutant mice (PubMed:10670447).2 Publications

Chemistry databases

ChEMBLiCHEMBL2094133

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000046326 – 473Gamma-aminobutyric acid receptor subunit beta-3Add BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP63080
PaxDbiP63080
PeptideAtlasiP63080
PRIDEiP63080

PTM databases

iPTMnetiP63080
PhosphoSitePlusiP63080

Expressioni

Gene expression databases

BgeeiENSMUSG00000033676
ExpressionAtlasiP63080 baseline and differential
GenevisibleiP63080 MM

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Can form functional homopentamers (in vitro). Interacts with UBQLN1. May interact with KIF21B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199804, 1 interactor
STRINGi10090.ENSMUSP00000038051

Structurei

3D structure databases

ProteinModelPortaliP63080
SMRiP63080
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 122Agonist bindingBy similarity3
Regioni180 – 182Agonist bindingBy similarity3
Regioni290 – 311Allosteric effector bindingBy similarityAdd BLAST22

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643 Eukaryota
ENOG410XPWH LUCA
GeneTreeiENSGT00760000118821
HOGENOMiHOG000231335
HOVERGENiHBG051707
InParanoidiP63080
KOiK05181

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006028 GABAA/Glycine_rcpt
IPR002289 GABAAb_rcpt
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01160 GABAARBETA
PR00253 GABAARECEPTR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGFAGGRLF GIFSAPVLVA VVCCAQSVND PGNMSFVKET VDKLLKGYDI
60 70 80 90 100
RLRPDFGGPP VCVGMNIDIA SIDMVSEVNM DYTLTMYFQQ YWRDKRLAYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWRGGDKAV
210 220 230 240 250
TGVERIELPQ FSIVEHRLVS RNVVFATGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSILITIL SWVSFWINYD ASAARVALGI TTVLTMTTIN THLRETLPKI
310 320 330 340 350
PYVKAIDMYL MGCFVFVFLA LLEYAFVNYI FFGRGPQRQK KLAEKTAKAK
360 370 380 390 400
NDRSKSEINR VDAHGNILLA PMDVHNEMNE VAGSVGDTRN SAISFDNSGI
410 420 430 440 450
QYRKQSMPKE GHGRYMGDRS IPHKKTHLRR RSSQLKIKIP DLTDVNAIDR
460 470
WSRIVFPFTF SLFNLVYWLY YVN
Length:473
Mass (Da):54,166
Last modified:September 13, 2004 - v1
Checksum:i6A5ABD8A9143FE45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14420 mRNA Translation: AAB60502.1
AK039070 mRNA Translation: BAC30230.1
AK147247 mRNA Translation: BAE27794.1
CCDSiCCDS21324.1
PIRiS53532
RefSeqiNP_032097.1, NM_008071.3
UniGeneiMm.8004

Genome annotation databases

EnsembliENSMUST00000039697; ENSMUSP00000038051; ENSMUSG00000033676
GeneIDi14402
KEGGimmu:14402
UCSCiuc009hee.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGBRB3_MOUSE
AccessioniPrimary (citable) accession number: P63080
Secondary accession number(s): P15433, Q3UHR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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