Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gamma-aminobutyric acid receptor subunit beta-3

Gene

Gabrb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251AgonistBy similarity

GO - Molecular functioni

  • AP-2 adaptor complex binding Source: RGD
  • chloride channel activity Source: RGD
  • GABA-A receptor activity Source: RGD
  • GABA-gated chloride ion channel activity Source: UniProtKB

GO - Biological processi

  • cellular response to histamine Source: UniProtKB
  • chloride transmembrane transport Source: GOC
  • palate development Source: UniProtKB
  • signal transduction Source: GOC
  • synaptic transmission, GABAergic Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-3
Alternative name(s):
GABA(A) receptor subunit beta-3
Gene namesi
Name:Gabrb3
Synonyms:Gabrb-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2651. Gabrb3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 245220ExtracellularBy similarityAdd
BLAST
Transmembranei246 – 26722HelicalBy similarityAdd
BLAST
Topological domaini268 – 2703CytoplasmicBy similarity
Transmembranei271 – 29323HelicalBy similarityAdd
BLAST
Topological domaini294 – 30411ExtracellularBy similarityAdd
BLAST
Transmembranei305 – 32723HelicalBy similarityAdd
BLAST
Topological domaini328 – 450123CytoplasmicBy similarityAdd
BLAST
Transmembranei451 – 47222HelicalBy similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • GABA-A receptor complex Source: UniProtKB
  • inhibitory synapse Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907607.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 473448Gamma-aminobutyric acid receptor subunit beta-3PRO_0000000464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence analysis
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP63079.
PRIDEiP63079.

Interactioni

Subunit structurei

Can form functional homopentamers (in vitro) (By similarity). Binds UBQLN1. Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arfgef2Q7TSU13EBI-6257937,EBI-6257913

GO - Molecular functioni

  • AP-2 adaptor complex binding Source: RGD

Protein-protein interaction databases

DIPiDIP-60770N.
IntActiP63079. 4 interactions.
STRINGi10116.ENSRNOP00000061969.

Chemistry

BindingDBiP63079.

Structurei

3D structure databases

ProteinModelPortaliP63079.
SMRiP63079. Positions 26-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 1223Agonist bindingBy similarity
Regioni180 – 1823Agonist bindingBy similarity
Regioni290 – 31122Allosteric effector bindingBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63079.
KOiK05181.
PhylomeDBiP63079.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGFAGGRLF GIFSAPVLVA VVCCAQSVND PGNMSFVKET VDKLLKGYDI
60 70 80 90 100
RLRPDFGGPP VCVGMNIDIA SIDMVSEVNM DYTLTMYFQQ YWRDKRLAYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWRGGDKAV
210 220 230 240 250
TGVERIELPQ FSIVEHRLVS RNVVFATGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSILITIL SWVSFWINYD ASAARVALGI TTVLTMTTIN THLRETLPKI
310 320 330 340 350
PYVKAIDMYL MGCFVFVFLA LLEYAFVNYI FFGRGPQRQK KLAEKTAKAK
360 370 380 390 400
NDRSKSEINR VDAHGNILLA PMDVHNEMNE VAGSVGDTRN SAISFDNSGI
410 420 430 440 450
QYRKQSMPKE GHGRYMGDRS IPHKKTHLRR RSSQLKIKIP DLTDVNAIDR
460 470
WSRIVFPFTF SLFNLVYWLY YVN
Length:473
Mass (Da):54,166
Last modified:September 13, 2004 - v1
Checksum:i6A5ABD8A9143FE45
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2561L → M in CAA33495 (PubMed:2548852).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15468 mRNA. Translation: CAA33495.1.
L04310 Genomic DNA. Translation: AAA41180.1.
PIRiS04466.
RefSeqiNP_058761.1. NM_017065.1.
UniGeneiRn.233948.

Genome annotation databases

GeneIDi24922.
KEGGirno:24922.
UCSCiRGD:2651. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15468 mRNA. Translation: CAA33495.1.
L04310 Genomic DNA. Translation: AAA41180.1.
PIRiS04466.
RefSeqiNP_058761.1. NM_017065.1.
UniGeneiRn.233948.

3D structure databases

ProteinModelPortaliP63079.
SMRiP63079. Positions 26-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60770N.
IntActiP63079. 4 interactions.
STRINGi10116.ENSRNOP00000061969.

Chemistry

BindingDBiP63079.
ChEMBLiCHEMBL1907607.

Proteomic databases

PaxDbiP63079.
PRIDEiP63079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24922.
KEGGirno:24922.
UCSCiRGD:2651. rat.

Organism-specific databases

CTDi2562.
RGDi2651. Gabrb3.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP63079.
KOiK05181.
PhylomeDBiP63079.

Miscellaneous databases

NextBioi285953.
PROiP63079.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs."
    Ymer S., Schofield P.R., Draguhn A., Werner P., Koehler M., Seeburg P.H.
    EMBO J. 8:1665-1670(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
  3. "A strong promoter element is located between alternative exons of a gene encoding the human gamma-aminobutyric acid-type A receptor beta 3 subunit (GABRB3)."
    Kirkness E.F., Fraser C.M.
    J. Biol. Chem. 268:4420-4428(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "Stoichiometry and assembly of a recombinant GABAA receptor subtype."
    Tretter V., Ehya N., Fuchs K., Sieghart W.
    J. Neurosci. 17:2728-2737(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT.
  5. "GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1."
    Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J.
    Nat. Neurosci. 4:908-916(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBQLN1.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  6. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGBRB3_RAT
AccessioniPrimary (citable) accession number: P63079
Secondary accession number(s): P15433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.