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Protein

Eukaryotic translation initiation factor 4E

Gene

Eif4e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity). Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • behavioral fear response Source: MGI
  • cellular response to dexamethasone stimulus Source: MGI
  • G1/S transition of mitotic cell cycle Source: MGI
  • lung development Source: Ensembl
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of translation Source: MGI
  • positive regulation of mitotic cell cycle Source: MGI
  • regulation of translation Source: UniProtKB
  • stem cell population maintenance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1169408. ISG15 antiviral mechanism.
R-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-166208. mTORC1-mediated signalling.
R-MMU-429947. Deadenylation of mRNA.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E
Short name:
eIF-4E
Short name:
eIF4E
Short name:
mRNA cap-binding protein
Alternative name(s):
eIF-4F 25 kDa subunit
Gene namesi
Name:Eif4e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95305. Eif4e.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73W → A: Binding to CYFIP1 reduced by 70%. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL6148.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001936352 – 217Eukaryotic translation initiation factor 4EAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei22PhosphothreonineBy similarity1
Modified residuei209Phosphoserine; by PKC and MKNK21 Publication1

Post-translational modificationi

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63073.
PaxDbiP63073.
PeptideAtlasiP63073.
PRIDEiP63073.

2D gel databases

REPRODUCTION-2DPAGEP63073.

PTM databases

iPTMnetiP63073.
PhosphoSitePlusiP63073.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028156.
CleanExiMM_EIF4E.
ExpressionAtlasiP63073. baseline and differential.
GenevisibleiP63073. MM.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Binds to MKNK2 in nucleus (By similarity). Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with LIMD1, WTIP and AJUBA (By similarity). Interacts with APOBEC3G in an RNA-dependent manner. Interacts with LARP1 (By similarity). Interacts with CLOCK. Interacts with METTL3 (By similarity).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CYFIP1Q7L5762EBI-2000006,EBI-1048143From a different organism.
Cyfip1Q7TMB85EBI-2000006,EBI-772928
Eif4g1Q6NZJ64EBI-2000006,EBI-8175606

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199420. 9 interactors.
DIPiDIP-42768N.
IntActiP63073. 6 interactors.
MINTiMINT-1856981.
STRINGi10090.ENSMUSP00000029803.

Chemistry databases

BindingDBiP63073.

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 34Combined sources4
Beta strandi38 – 49Combined sources12
Beta strandi52 – 54Combined sources3
Helixi57 – 59Combined sources3
Beta strandi60 – 68Combined sources9
Helixi69 – 76Combined sources8
Helixi82 – 84Combined sources3
Beta strandi89 – 95Combined sources7
Turni105 – 109Combined sources5
Beta strandi111 – 118Combined sources8
Helixi119 – 121Combined sources3
Turni122 – 125Combined sources4
Helixi126 – 138Combined sources13
Turni139 – 142Combined sources4
Helixi143 – 148Combined sources6
Beta strandi149 – 156Combined sources8
Beta strandi158 – 168Combined sources11
Helixi173 – 187Combined sources15
Turni191 – 193Combined sources3
Beta strandi195 – 199Combined sources5
Helixi200 – 204Combined sources5
Beta strandi208 – 210Combined sources3
Beta strandi214 – 217Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EJ1X-ray2.20A/B28-217[»]
1EJ4X-ray2.25A28-217[»]
1EJHX-ray2.20A/B/C/D28-217[»]
1L8BX-ray1.80A/B28-217[»]
5BXVX-ray2.10A/C27-217[»]
ProteinModelPortaliP63073.
SMRiP63073.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63073.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 40EIF4EBP1/2/3 binding4
Regioni56 – 577-methylguanosine-containing mRNA cap binding2
Regioni73 – 77EIF4EBP1/2/3 binding5
Regioni102 – 1037-methylguanosine-containing mRNA cap binding2
Regioni132 – 139EIF4EBP1/2/3 binding8
Regioni157 – 1627-methylguanosine-containing mRNA cap binding6
Regioni205 – 2077-methylguanosine-containing mRNA cap binding3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1670. Eukaryota.
COG5053. LUCA.
GeneTreeiENSGT00520000055549.
HOGENOMiHOG000186751.
HOVERGENiHBG006130.
InParanoidiP63073.
KOiK03259.
OMAiWTLWYYE.
OrthoDBiEOG091G0IVA.
PhylomeDBiP63073.
TreeFamiTF101526.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVEPETTP TTNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN
60 70 80 90 100
DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP
110 120 130 140 150
MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC
160 170 180 190 200
GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG RVYKERLGLP PKIVIGYQSH
210
ADTATKSGST TKNRFVV
Length:217
Mass (Da):25,053
Last modified:September 13, 2004 - v1
Checksum:iFC61D0FB337BCD8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70E → L no nucleotide entry (PubMed:2663851).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61731 mRNA. Translation: AAA37545.1.
BC010759 mRNA. Translation: AAH10759.1.
BC085087 mRNA. Translation: AAH85087.1.
CCDSiCCDS38654.1.
PIRiA34295.
I49644.
RefSeqiNP_031943.3. NM_007917.4.
UniGeneiMm.3941.
Mm.488704.

Genome annotation databases

EnsembliENSMUST00000029803; ENSMUSP00000029803; ENSMUSG00000028156.
GeneIDi13684.
KEGGimmu:13684.
UCSCiuc008rnn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61731 mRNA. Translation: AAA37545.1.
BC010759 mRNA. Translation: AAH10759.1.
BC085087 mRNA. Translation: AAH85087.1.
CCDSiCCDS38654.1.
PIRiA34295.
I49644.
RefSeqiNP_031943.3. NM_007917.4.
UniGeneiMm.3941.
Mm.488704.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EJ1X-ray2.20A/B28-217[»]
1EJ4X-ray2.25A28-217[»]
1EJHX-ray2.20A/B/C/D28-217[»]
1L8BX-ray1.80A/B28-217[»]
5BXVX-ray2.10A/C27-217[»]
ProteinModelPortaliP63073.
SMRiP63073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199420. 9 interactors.
DIPiDIP-42768N.
IntActiP63073. 6 interactors.
MINTiMINT-1856981.
STRINGi10090.ENSMUSP00000029803.

Chemistry databases

BindingDBiP63073.
ChEMBLiCHEMBL6148.

PTM databases

iPTMnetiP63073.
PhosphoSitePlusiP63073.

2D gel databases

REPRODUCTION-2DPAGEP63073.

Proteomic databases

EPDiP63073.
PaxDbiP63073.
PeptideAtlasiP63073.
PRIDEiP63073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029803; ENSMUSP00000029803; ENSMUSG00000028156.
GeneIDi13684.
KEGGimmu:13684.
UCSCiuc008rnn.1. mouse.

Organism-specific databases

CTDi1977.
MGIiMGI:95305. Eif4e.

Phylogenomic databases

eggNOGiKOG1670. Eukaryota.
COG5053. LUCA.
GeneTreeiENSGT00520000055549.
HOGENOMiHOG000186751.
HOVERGENiHBG006130.
InParanoidiP63073.
KOiK03259.
OMAiWTLWYYE.
OrthoDBiEOG091G0IVA.
PhylomeDBiP63073.
TreeFamiTF101526.

Enzyme and pathway databases

ReactomeiR-MMU-1169408. ISG15 antiviral mechanism.
R-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-166208. mTORC1-mediated signalling.
R-MMU-429947. Deadenylation of mRNA.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

EvolutionaryTraceiP63073.
PROiP63073.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028156.
CleanExiMM_EIF4E.
ExpressionAtlasiP63073. baseline and differential.
GenevisibleiP63073. MM.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4E_MOUSE
AccessioniPrimary (citable) accession number: P63073
Secondary accession number(s): P20415
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.