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P63073

- IF4E_MOUSE

UniProt

P63073 - IF4E_MOUSE

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Protein

Eukaryotic translation initiation factor 4E

Gene

Eif4e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity). Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.By similarity1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl
  2. translation initiation factor activity Source: UniProtKB

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: Ensembl
  2. lung development Source: Ensembl
  3. positive regulation of mitotic cell cycle Source: Ensembl
  4. regulation of translation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_198533. ISG15 antiviral mechanism.
REACT_212343. Transport of the SLBP independent Mature mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E
Short name:
eIF-4E
Short name:
eIF4E
Short name:
mRNA cap-binding protein
Alternative name(s):
eIF-4F 25 kDa subunit
Gene namesi
Name:Eif4e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:95305. Eif4e.

Subcellular locationi

CytoplasmP-body By similarity. Cytoplasm 1 Publication

GO - Cellular componenti

  1. chromatoid body Source: MGI
  2. cytoplasm Source: MGI
  3. cytoplasmic mRNA processing body Source: Ensembl
  4. cytoplasmic stress granule Source: UniProtKB
  5. eukaryotic translation initiation factor 4F complex Source: UniProtKB
  6. extracellular vesicular exosome Source: Ensembl
  7. mRNA cap binding complex Source: UniProtKB
  8. RISC complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731W → A: Binding to CYFIP1 reduced by 70%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 217216Eukaryotic translation initiation factor 4EPRO_0000193635Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei209 – 2091Phosphoserine; by PKC and MKNK21 Publication

Post-translational modificationi

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP63073.
PaxDbiP63073.
PRIDEiP63073.

2D gel databases

REPRODUCTION-2DPAGEP63073.

PTM databases

PhosphoSiteiP63073.

Expressioni

Gene expression databases

BgeeiP63073.
CleanExiMM_EIF4E.
ExpressionAtlasiP63073. baseline and differential.
GenevestigatoriP63073.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Binds to MKNK2 in nucleus (By similarity). Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with LIMD1, WTIP and AJUBA (By similarity). Interacts with APOBEC3G in an RNA-dependent manner. Interacts with LARP1 (By similarity). Interacts with CLOCK.By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CYFIP1Q7L5762EBI-2000006,EBI-1048143From a different organism.
Cyfip1Q7TMB85EBI-2000006,EBI-772928
Eif4g1Q6NZJ64EBI-2000006,EBI-8175606

Protein-protein interaction databases

BioGridi199420. 10 interactions.
DIPiDIP-42768N.
IntActiP63073. 6 interactions.
MINTiMINT-1856981.
STRINGi10090.ENSMUSP00000029803.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi38 – 4912Combined sources
Beta strandi52 – 543Combined sources
Helixi57 – 593Combined sources
Beta strandi60 – 689Combined sources
Helixi69 – 768Combined sources
Helixi82 – 843Combined sources
Beta strandi89 – 957Combined sources
Turni105 – 1095Combined sources
Beta strandi111 – 1188Combined sources
Helixi119 – 1213Combined sources
Turni122 – 1254Combined sources
Helixi126 – 13813Combined sources
Turni139 – 1424Combined sources
Helixi143 – 1486Combined sources
Beta strandi149 – 1568Combined sources
Beta strandi158 – 16811Combined sources
Helixi173 – 18715Combined sources
Turni191 – 1933Combined sources
Beta strandi195 – 1995Combined sources
Helixi200 – 2045Combined sources
Beta strandi214 – 2174Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EJ1X-ray2.20A/B28-217[»]
1EJ4X-ray2.25A28-217[»]
1EJHX-ray2.20A/B/C/D28-217[»]
1L8BX-ray1.80A/B28-217[»]
ProteinModelPortaliP63073.
SMRiP63073. Positions 1-217.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63073.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 404EIF4EBP1/2/3 binding
Regioni56 – 5727-methylguanosine-containing mRNA cap binding
Regioni73 – 775EIF4EBP1/2/3 binding
Regioni102 – 10327-methylguanosine-containing mRNA cap binding
Regioni132 – 1398EIF4EBP1/2/3 binding
Regioni157 – 16267-methylguanosine-containing mRNA cap binding
Regioni205 – 20737-methylguanosine-containing mRNA cap binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5053.
HOGENOMiHOG000186751.
HOVERGENiHBG006130.
InParanoidiP63073.
KOiK03259.
OMAiVASPEQY.
OrthoDBiEOG7QRQVM.
PhylomeDBiP63073.
TreeFamiTF101526.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like_dom.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63073-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATVEPETTP TTNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN
60 70 80 90 100
DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP
110 120 130 140 150
MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC
160 170 180 190 200
GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG RVYKERLGLP PKIVIGYQSH
210
ADTATKSGST TKNRFVV
Length:217
Mass (Da):25,053
Last modified:September 13, 2004 - v1
Checksum:iFC61D0FB337BCD8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701E → L no nucleotide entry (PubMed:2663851)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M61731 mRNA. Translation: AAA37545.1.
BC010759 mRNA. Translation: AAH10759.1.
BC085087 mRNA. Translation: AAH85087.1.
CCDSiCCDS38654.1.
PIRiA34295.
I49644.
RefSeqiNP_031943.3. NM_007917.3.
UniGeneiMm.3941.
Mm.488704.

Genome annotation databases

EnsembliENSMUST00000029803; ENSMUSP00000029803; ENSMUSG00000028156.
GeneIDi13684.
KEGGimmu:13684.
UCSCiuc008rnn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M61731 mRNA. Translation: AAA37545.1 .
BC010759 mRNA. Translation: AAH10759.1 .
BC085087 mRNA. Translation: AAH85087.1 .
CCDSi CCDS38654.1.
PIRi A34295.
I49644.
RefSeqi NP_031943.3. NM_007917.3.
UniGenei Mm.3941.
Mm.488704.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EJ1 X-ray 2.20 A/B 28-217 [» ]
1EJ4 X-ray 2.25 A 28-217 [» ]
1EJH X-ray 2.20 A/B/C/D 28-217 [» ]
1L8B X-ray 1.80 A/B 28-217 [» ]
ProteinModelPortali P63073.
SMRi P63073. Positions 1-217.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199420. 10 interactions.
DIPi DIP-42768N.
IntActi P63073. 6 interactions.
MINTi MINT-1856981.
STRINGi 10090.ENSMUSP00000029803.

Chemistry

BindingDBi P63073.
ChEMBLi CHEMBL6148.

PTM databases

PhosphoSitei P63073.

2D gel databases

REPRODUCTION-2DPAGE P63073.

Proteomic databases

MaxQBi P63073.
PaxDbi P63073.
PRIDEi P63073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029803 ; ENSMUSP00000029803 ; ENSMUSG00000028156 .
GeneIDi 13684.
KEGGi mmu:13684.
UCSCi uc008rnn.1. mouse.

Organism-specific databases

CTDi 1977.
MGIi MGI:95305. Eif4e.

Phylogenomic databases

eggNOGi COG5053.
HOGENOMi HOG000186751.
HOVERGENi HBG006130.
InParanoidi P63073.
KOi K03259.
OMAi VASPEQY.
OrthoDBi EOG7QRQVM.
PhylomeDBi P63073.
TreeFami TF101526.

Enzyme and pathway databases

Reactomei REACT_198533. ISG15 antiviral mechanism.
REACT_212343. Transport of the SLBP independent Mature mRNA.

Miscellaneous databases

EvolutionaryTracei P63073.
NextBioi 284446.
PROi P63073.
SOURCEi Search...

Gene expression databases

Bgeei P63073.
CleanExi MM_EIF4E.
ExpressionAtlasi P63073. baseline and differential.
Genevestigatori P63073.

Family and domain databases

Gene3Di 3.30.760.10. 1 hit.
InterProi IPR023398. TIF_eIF4e-like_dom.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view ]
PANTHERi PTHR11960. PTHR11960. 1 hit.
Pfami PF01652. IF4E. 1 hit.
[Graphical view ]
SUPFAMi SSF55418. SSF55418. 1 hit.
PROSITEi PS00813. IF4E. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A mammalian translation initiation factor can substitute for its yeast homologue in vivo."
    Altmann M., Mueller P.P., Pelletier J., Sonenberg N., Trachsel H.
    J. Biol. Chem. 264:12145-12147(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Multiple mRNAs encode the murine translation initiation factor eIF-4E."
    Jaramillo M., Pelletier J., Edery I., Nielsen P.J., Sonenberg N.
    J. Biol. Chem. 266:10446-10451(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary gland.
  4. "Translational control by neuroguidin, a eukaryotic initiation factor 4E and CPEB binding protein."
    Jung M.-Y., Lorenz L., Richter J.D.
    Mol. Cell. Biol. 26:4277-4287(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NGDN.
  5. "Roles of mitogen-activated protein kinase signal-integrating kinases 1 and 2 in oxidant-mediated eIF4E phosphorylation."
    Shenberger J.S., Zhang L., Hughlock M.K., Ueda T., Watanabe-Fukunaga R., Fukunaga R.
    Int. J. Biochem. Cell Biol. 39:1828-1842(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-209 BY MKNK2.
  6. "The fragile X syndrome protein represses activity-dependent translation through CYFIP1, a new 4E-BP."
    Napoli I., Mercaldo V., Boyl P.P., Eleuteri B., Zalfa F., De Rubeis S., Di Marino D., Mohr E., Massimi M., Falconi M., Witke W., Costa-Mattioli M., Sonenberg N., Achsel T., Bagni C.
    Cell 134:1042-1054(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CYFIP1 AND FMR1, MUTAGENESIS OF TRP-73.
  7. "MILI, a PIWI-interacting RNA-binding protein, is required for germ Line stem cell self-renewal and appears to positively regulate translation."
    Unhavaithaya Y., Hao Y., Beyret E., Yin H., Kuramochi-Miyagawa S., Nakano T., Lin H.
    J. Biol. Chem. 284:6507-6519(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIWIL2.
  8. "Circadian proteins CLOCK and BMAL1 in the chromatoid body, a RNA processing granule of male germ cells."
    Peruquetti R.L., de Mateo S., Sassone-Corsi P.
    PLoS ONE 7:E42695-E42695(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CLOCK.
  9. "Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP."
    Marcotrigiano J., Gingras A.-C., Sonenberg N., Burley S.K.
    Cell 89:951-961(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 28-217 IN COMPLEX WITH MRNA CAP ANALOG.
  10. "Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G."
    Marcotrigiano J., Gingras A.-C., Sonenberg N., Burley S.K.
    Mol. Cell 3:707-716(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 28-217 IN COMPLEX WITH MRNA CAP ANALOG AND EIF4EBP1.

Entry informationi

Entry nameiIF4E_MOUSE
AccessioniPrimary (citable) accession number: P63073
Secondary accession number(s): P20415
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3