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P63073

- IF4E_MOUSE

UniProt

P63073 - IF4E_MOUSE

Protein

Eukaryotic translation initiation factor 4E

Gene

Eif4e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development By similarity. Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.By similarity1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. translation initiation factor activity Source: UniProtKB

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: Ensembl
    2. lung development Source: Ensembl
    3. positive regulation of mitotic cell cycle Source: Ensembl
    4. regulation of translation Source: UniProtKB

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198533. ISG15 antiviral mechanism.
    REACT_212343. Transport of the SLBP independent Mature mRNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 4E
    Short name:
    eIF-4E
    Short name:
    eIF4E
    Short name:
    mRNA cap-binding protein
    Alternative name(s):
    eIF-4F 25 kDa subunit
    Gene namesi
    Name:Eif4e
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:95305. Eif4e.

    Subcellular locationi

    CytoplasmP-body By similarity. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. chromatoid body Source: MGI
    2. cytoplasm Source: MGI
    3. cytoplasmic mRNA processing body Source: UniProtKB-SubCell
    4. cytoplasmic stress granule Source: UniProtKB
    5. eukaryotic translation initiation factor 4F complex Source: UniProtKB
    6. mRNA cap binding complex Source: UniProtKB
    7. RISC complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi73 – 731W → A: Binding to CYFIP1 reduced by 70%. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 217216Eukaryotic translation initiation factor 4EPRO_0000193635Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei209 – 2091Phosphoserine; by PKC and MKNK21 Publication

    Post-translational modificationi

    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP63073.
    PaxDbiP63073.
    PRIDEiP63073.

    2D gel databases

    REPRODUCTION-2DPAGEP63073.

    PTM databases

    PhosphoSiteiP63073.

    Expressioni

    Gene expression databases

    BgeeiP63073.
    CleanExiMM_EIF4E.
    GenevestigatoriP63073.

    Interactioni

    Subunit structurei

    eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Binds to MKNK2 in nucleus By similarity. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with LIMD1, WTIP and AJUBA By similarity. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with LARP1 By similarity. Interacts with CLOCK.By similarity6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CYFIP1Q7L5762EBI-2000006,EBI-1048143From a different organism.
    Cyfip1Q7TMB85EBI-2000006,EBI-772928
    Eif4g1Q6NZJ64EBI-2000006,EBI-8175606

    Protein-protein interaction databases

    BioGridi199420. 10 interactions.
    DIPiDIP-42768N.
    IntActiP63073. 6 interactions.
    MINTiMINT-1856981.
    STRINGi10090.ENSMUSP00000029803.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi38 – 4912
    Beta strandi52 – 543
    Helixi57 – 593
    Beta strandi60 – 689
    Helixi69 – 768
    Helixi82 – 843
    Beta strandi89 – 957
    Turni105 – 1095
    Beta strandi111 – 1188
    Helixi119 – 1213
    Turni122 – 1254
    Helixi126 – 13813
    Turni139 – 1424
    Helixi143 – 1486
    Beta strandi149 – 1568
    Beta strandi158 – 16811
    Helixi173 – 18715
    Turni191 – 1933
    Beta strandi195 – 1995
    Helixi200 – 2045
    Beta strandi214 – 2174

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EJ1X-ray2.20A/B28-217[»]
    1EJ4X-ray2.25A28-217[»]
    1EJHX-ray2.20A/B/C/D28-217[»]
    1L8BX-ray1.80A/B28-217[»]
    ProteinModelPortaliP63073.
    SMRiP63073. Positions 1-217.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63073.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni37 – 404EIF4EBP1/2/3 binding
    Regioni56 – 5727-methylguanosine-containing mRNA cap binding
    Regioni73 – 775EIF4EBP1/2/3 binding
    Regioni102 – 10327-methylguanosine-containing mRNA cap binding
    Regioni132 – 1398EIF4EBP1/2/3 binding
    Regioni157 – 16267-methylguanosine-containing mRNA cap binding
    Regioni205 – 20737-methylguanosine-containing mRNA cap binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5053.
    HOGENOMiHOG000186751.
    HOVERGENiHBG006130.
    InParanoidiP63073.
    KOiK03259.
    OMAiVASPEQY.
    OrthoDBiEOG7QRQVM.
    PhylomeDBiP63073.
    TreeFamiTF101526.

    Family and domain databases

    Gene3Di3.30.760.10. 1 hit.
    InterProiIPR023398. TIF_eIF4e-like_dom.
    IPR001040. TIF_eIF_4E.
    IPR019770. TIF_eIF_4E_CS.
    [Graphical view]
    PANTHERiPTHR11960. PTHR11960. 1 hit.
    PfamiPF01652. IF4E. 1 hit.
    [Graphical view]
    SUPFAMiSSF55418. SSF55418. 1 hit.
    PROSITEiPS00813. IF4E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63073-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATVEPETTP TTNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN    50
    DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP 100
    MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC 150
    GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG RVYKERLGLP PKIVIGYQSH 200
    ADTATKSGST TKNRFVV 217
    Length:217
    Mass (Da):25,053
    Last modified:September 13, 2004 - v1
    Checksum:iFC61D0FB337BCD8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701E → L no nucleotide entry (PubMed:2663851)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61731 mRNA. Translation: AAA37545.1.
    BC010759 mRNA. Translation: AAH10759.1.
    BC085087 mRNA. Translation: AAH85087.1.
    CCDSiCCDS38654.1.
    PIRiA34295.
    I49644.
    RefSeqiNP_031943.3. NM_007917.3.
    UniGeneiMm.3941.
    Mm.488704.

    Genome annotation databases

    EnsembliENSMUST00000029803; ENSMUSP00000029803; ENSMUSG00000028156.
    GeneIDi13684.
    KEGGimmu:13684.
    UCSCiuc008rnn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61731 mRNA. Translation: AAA37545.1 .
    BC010759 mRNA. Translation: AAH10759.1 .
    BC085087 mRNA. Translation: AAH85087.1 .
    CCDSi CCDS38654.1.
    PIRi A34295.
    I49644.
    RefSeqi NP_031943.3. NM_007917.3.
    UniGenei Mm.3941.
    Mm.488704.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EJ1 X-ray 2.20 A/B 28-217 [» ]
    1EJ4 X-ray 2.25 A 28-217 [» ]
    1EJH X-ray 2.20 A/B/C/D 28-217 [» ]
    1L8B X-ray 1.80 A/B 28-217 [» ]
    ProteinModelPortali P63073.
    SMRi P63073. Positions 1-217.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199420. 10 interactions.
    DIPi DIP-42768N.
    IntActi P63073. 6 interactions.
    MINTi MINT-1856981.
    STRINGi 10090.ENSMUSP00000029803.

    Chemistry

    BindingDBi P63073.
    ChEMBLi CHEMBL6148.

    PTM databases

    PhosphoSitei P63073.

    2D gel databases

    REPRODUCTION-2DPAGE P63073.

    Proteomic databases

    MaxQBi P63073.
    PaxDbi P63073.
    PRIDEi P63073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029803 ; ENSMUSP00000029803 ; ENSMUSG00000028156 .
    GeneIDi 13684.
    KEGGi mmu:13684.
    UCSCi uc008rnn.1. mouse.

    Organism-specific databases

    CTDi 1977.
    MGIi MGI:95305. Eif4e.

    Phylogenomic databases

    eggNOGi COG5053.
    HOGENOMi HOG000186751.
    HOVERGENi HBG006130.
    InParanoidi P63073.
    KOi K03259.
    OMAi VASPEQY.
    OrthoDBi EOG7QRQVM.
    PhylomeDBi P63073.
    TreeFami TF101526.

    Enzyme and pathway databases

    Reactomei REACT_198533. ISG15 antiviral mechanism.
    REACT_212343. Transport of the SLBP independent Mature mRNA.

    Miscellaneous databases

    EvolutionaryTracei P63073.
    NextBioi 284446.
    PROi P63073.
    SOURCEi Search...

    Gene expression databases

    Bgeei P63073.
    CleanExi MM_EIF4E.
    Genevestigatori P63073.

    Family and domain databases

    Gene3Di 3.30.760.10. 1 hit.
    InterProi IPR023398. TIF_eIF4e-like_dom.
    IPR001040. TIF_eIF_4E.
    IPR019770. TIF_eIF_4E_CS.
    [Graphical view ]
    PANTHERi PTHR11960. PTHR11960. 1 hit.
    Pfami PF01652. IF4E. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55418. SSF55418. 1 hit.
    PROSITEi PS00813. IF4E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A mammalian translation initiation factor can substitute for its yeast homologue in vivo."
      Altmann M., Mueller P.P., Pelletier J., Sonenberg N., Trachsel H.
      J. Biol. Chem. 264:12145-12147(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Multiple mRNAs encode the murine translation initiation factor eIF-4E."
      Jaramillo M., Pelletier J., Edery I., Nielsen P.J., Sonenberg N.
      J. Biol. Chem. 266:10446-10451(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Mammary gland.
    4. "Translational control by neuroguidin, a eukaryotic initiation factor 4E and CPEB binding protein."
      Jung M.-Y., Lorenz L., Richter J.D.
      Mol. Cell. Biol. 26:4277-4287(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NGDN.
    5. "Roles of mitogen-activated protein kinase signal-integrating kinases 1 and 2 in oxidant-mediated eIF4E phosphorylation."
      Shenberger J.S., Zhang L., Hughlock M.K., Ueda T., Watanabe-Fukunaga R., Fukunaga R.
      Int. J. Biochem. Cell Biol. 39:1828-1842(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-209 BY MKNK2.
    6. "The fragile X syndrome protein represses activity-dependent translation through CYFIP1, a new 4E-BP."
      Napoli I., Mercaldo V., Boyl P.P., Eleuteri B., Zalfa F., De Rubeis S., Di Marino D., Mohr E., Massimi M., Falconi M., Witke W., Costa-Mattioli M., Sonenberg N., Achsel T., Bagni C.
      Cell 134:1042-1054(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CYFIP1 AND FMR1, MUTAGENESIS OF TRP-73.
    7. "MILI, a PIWI-interacting RNA-binding protein, is required for germ Line stem cell self-renewal and appears to positively regulate translation."
      Unhavaithaya Y., Hao Y., Beyret E., Yin H., Kuramochi-Miyagawa S., Nakano T., Lin H.
      J. Biol. Chem. 284:6507-6519(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIWIL2.
    8. "Circadian proteins CLOCK and BMAL1 in the chromatoid body, a RNA processing granule of male germ cells."
      Peruquetti R.L., de Mateo S., Sassone-Corsi P.
      PLoS ONE 7:E42695-E42695(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CLOCK.
    9. "Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP."
      Marcotrigiano J., Gingras A.-C., Sonenberg N., Burley S.K.
      Cell 89:951-961(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 28-217 IN COMPLEX WITH MRNA CAP ANALOG.
    10. "Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G."
      Marcotrigiano J., Gingras A.-C., Sonenberg N., Burley S.K.
      Mol. Cell 3:707-716(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 28-217 IN COMPLEX WITH MRNA CAP ANALOG AND EIF4EBP1.

    Entry informationi

    Entry nameiIF4E_MOUSE
    AccessioniPrimary (citable) accession number: P63073
    Secondary accession number(s): P20415
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3