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P63072

- UBD_MOUSE

UniProt

P63072 - UBD_MOUSE

Protein

Ubiquitin D

Gene

Ubd

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases.6 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei161 – 1622Activation by thioester intermediate formation with UBA6

    GO - Molecular functioni

    1. proteasome binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. aggresome assembly Source: UniProtKB
    2. myeloid dendritic cell differentiation Source: UniProtKB
    3. positive regulation of apoptotic process Source: UniProtKB
    4. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    5. protein modification by small protein conjugation Source: UniProtKB
    6. protein ubiquitination Source: UniProtKB
    7. response to interferon-gamma Source: UniProtKB
    8. response to organonitrogen compound Source: Ensembl
    9. response to tumor necrosis factor Source: UniProtKB
    10. ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin D
    Alternative name(s):
    Diubiquitin
    Ubiquitin-like protein FAT10
    Gene namesi
    Name:Ubd
    Synonyms:Fat10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1344410. Ubd.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication
    Note: Accumulates in aggresomes under proteasome inhibition conditions.By similarity

    GO - Cellular componenti

    1. aggresome Source: Ensembl
    2. cytoplasm Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Spontaneous sporadic apoptotic death. Higher sensitivity toward endotoxin challenge. Abrogated TNF-alpha-induced NF-kappa-B activation and reduced induction of NF-kappa-B-regulated genes. Impaired TNF-alpha-induced I-kappa-B-alpha degradation and nuclear translocation of p65 in RTECs. Reduced expression of LMP2.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi161 – 1622Missing: Impaired conjugation of target proteins. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 162162Ubiquitin DPRO_0000114894Add
    BLAST

    Post-translational modificationi

    Can be acetylated.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP63072.

    PTM databases

    PhosphoSiteiP63072.

    Expressioni

    Tissue specificityi

    Mostly expressed in thymus and intestine.1 Publication

    Inductioni

    Rapidly degraded by the proteasome. Cell-cycle regulation with highest expression during the S-phase (at protein level). Over expressed in hepatocytes by drug injury (e.g. DDC; diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate). Inducible by the proinflammatory cytokines tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg).3 Publications

    Gene expression databases

    BgeeiP63072.
    CleanExiMM_UBD.
    GenevestigatoriP63072.

    Interactioni

    Subunit structurei

    Interact directly with the 26S proteasome. The interaction with NUB1L vie the N-terminal ubiquitin domain facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates its own degradation and that of its conjugates By similarity. Interacts with the spindle checkpoint protein MAD2L1 during mitosis. Present in aggresomes of proteasome inhibited cells. Interacts with HDAC6 under proteasome impairment conditions. Forms a thioester with UBA6 in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg).By similarity1 Publication

    Protein-protein interaction databases

    BioGridi204900. 3 interactions.
    MINTiMINT-1341042.
    STRINGi10090.ENSMUSP00000035808.

    Structurei

    3D structure databases

    ProteinModelPortaliP63072.
    SMRiP63072. Positions 9-162.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 7876Ubiquitin-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini87 – 16074Ubiquitin-like 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ubiquitin-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG259502.
    GeneTreeiENSGT00750000117776.
    HOGENOMiHOG000134446.
    HOVERGENiHBG101094.
    InParanoidiQ4FJZ5.
    KOiK12157.
    OMAiTCNGKKL.
    OrthoDBiEOG75TMDT.
    PhylomeDBiP63072.

    Family and domain databases

    InterProiIPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00240. ubiquitin. 2 hits.
    [Graphical view]
    PRINTSiPR00348. UBIQUITIN.
    SMARTiSM00213. UBQ. 2 hits.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 2 hits.
    PROSITEiPS50053. UBIQUITIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63072-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVRTCVVR SDQWRLMTFE TTENDKVKKI NEHIRSQTKV SVQDQILLLD    50
    SKILKPHRKL SSYGIDKETT IHLTLKVVKP SDEELPLFLV ESKNEGQRHL 100
    LRVRRSSSVA QVKEMIESVT SVIPKKQVVN CNGKKLEDGK IMADYNIKSG 150
    SLLFLTTHCT GG 162
    Length:162
    Mass (Da):18,376
    Last modified:September 13, 2004 - v1
    Checksum:i6107D8604F96455F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF314088 Genomic DNA. Translation: AAG27477.1.
    AK008116 mRNA. Translation: BAB25471.1.
    AK008552 mRNA. Translation: BAB25738.1.
    CT010257 mRNA. Translation: CAJ18465.1.
    AL078630 Genomic DNA. No translation available.
    BC027627 mRNA. Translation: AAH27627.2.
    BC036383 mRNA. Translation: AAH36383.1.
    CCDSiCCDS28737.1.
    RefSeqiNP_075626.1. NM_023137.3.
    UniGeneiMm.140210.

    Genome annotation databases

    EnsembliENSMUST00000038844; ENSMUSP00000035808; ENSMUSG00000035186.
    GeneIDi24108.
    KEGGimmu:24108.
    UCSCiuc008cmi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF314088 Genomic DNA. Translation: AAG27477.1 .
    AK008116 mRNA. Translation: BAB25471.1 .
    AK008552 mRNA. Translation: BAB25738.1 .
    CT010257 mRNA. Translation: CAJ18465.1 .
    AL078630 Genomic DNA. No translation available.
    BC027627 mRNA. Translation: AAH27627.2 .
    BC036383 mRNA. Translation: AAH36383.1 .
    CCDSi CCDS28737.1.
    RefSeqi NP_075626.1. NM_023137.3.
    UniGenei Mm.140210.

    3D structure databases

    ProteinModelPortali P63072.
    SMRi P63072. Positions 9-162.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204900. 3 interactions.
    MINTi MINT-1341042.
    STRINGi 10090.ENSMUSP00000035808.

    PTM databases

    PhosphoSitei P63072.

    Proteomic databases

    PRIDEi P63072.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038844 ; ENSMUSP00000035808 ; ENSMUSG00000035186 .
    GeneIDi 24108.
    KEGGi mmu:24108.
    UCSCi uc008cmi.2. mouse.

    Organism-specific databases

    CTDi 10537.
    MGIi MGI:1344410. Ubd.

    Phylogenomic databases

    eggNOGi NOG259502.
    GeneTreei ENSGT00750000117776.
    HOGENOMi HOG000134446.
    HOVERGENi HBG101094.
    InParanoidi Q4FJZ5.
    KOi K12157.
    OMAi TCNGKKL.
    OrthoDBi EOG75TMDT.
    PhylomeDBi P63072.

    Miscellaneous databases

    ChiTaRSi UBD. mouse.
    NextBioi 304123.
    PROi P63072.
    SOURCEi Search...

    Gene expression databases

    Bgeei P63072.
    CleanExi MM_UBD.
    Genevestigatori P63072.

    Family and domain databases

    InterProi IPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00240. ubiquitin. 2 hits.
    [Graphical view ]
    PRINTSi PR00348. UBIQUITIN.
    SMARTi SM00213. UBQ. 2 hits.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 2 hits.
    PROSITEi PS50053. UBIQUITIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Disruption of the mouse Fat10 gene by gene targeting in the mouse embryonic stem cells."
      Yu X., Liu Y., Weissman S.M.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Small intestine.
    3. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
      Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    6. "The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis."
      Raasi S., Schmidtke G., Groettrup M.
      J. Biol. Chem. 276:35334-35343(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF 161-GLY-GLY-162.
    7. "FAT10, a ubiquitin-independent signal for proteasomal degradation."
      Hipp M.S., Kalveram B., Raasi S., Groettrup M., Schmidtke G.
      Mol. Cell. Biol. 25:3483-3491(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: FUNCTION.
    9. "FAT10/diubiquitin-like protein-deficient mice exhibit minimal phenotypic differences."
      Canaan A., Yu X., Booth C.J., Lian J., Lazar I., Gamfi S.L., Castille K., Kohya N., Nakayama Y., Liu Y.-C., Eynon E., Flavell R., Weissman S.M.
      Mol. Cell. Biol. 26:5180-5189(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY TNFA AND IFNG, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    10. "E1-L2 activates both ubiquitin and FAT10."
      Chiu Y.-H., Sun Q., Chen Z.J.
      Mol. Cell 27:1014-1023(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH UBA6.
    11. "Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model of tumorigenesis."
      Oliva J., Bardag-Gorce F., French B.A., Li J., McPhaul L., Amidi F., Dedes J., Habibi A., Nguyen S., French S.W.
      Exp. Mol. Pathol. 84:102-112(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY DRUG INJURY.
    12. Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiUBD_MOUSE
    AccessioniPrimary (citable) accession number: P63072
    Secondary accession number(s): Q4FJZ5, Q9WV10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3