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P63072

- UBD_MOUSE

UniProt

P63072 - UBD_MOUSE

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Protein

Ubiquitin D

Gene

Ubd

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases.6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei161 – 1622Activation by thioester intermediate formation with UBA6

GO - Molecular functioni

  1. proteasome binding Source: UniProtKB

GO - Biological processi

  1. aggresome assembly Source: UniProtKB
  2. myeloid dendritic cell differentiation Source: UniProtKB
  3. positive regulation of apoptotic process Source: UniProtKB
  4. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  5. protein modification by small protein conjugation Source: UniProtKB
  6. protein ubiquitination Source: UniProtKB
  7. response to interferon-gamma Source: UniProtKB
  8. response to organonitrogen compound Source: Ensembl
  9. response to tumor necrosis factor Source: UniProtKB
  10. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin D
Alternative name(s):
Diubiquitin
Ubiquitin-like protein FAT10
Gene namesi
Name:Ubd
Synonyms:Fat10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1344410. Ubd.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication
Note: Accumulates in aggresomes under proteasome inhibition conditions.By similarity

GO - Cellular componenti

  1. aggresome Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Spontaneous sporadic apoptotic death. Higher sensitivity toward endotoxin challenge. Abrogated TNF-alpha-induced NF-kappa-B activation and reduced induction of NF-kappa-B-regulated genes. Impaired TNF-alpha-induced I-kappa-B-alpha degradation and nuclear translocation of p65 in RTECs. Reduced expression of LMP2.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1622Missing: Impaired conjugation of target proteins. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162Ubiquitin DPRO_0000114894Add
BLAST

Post-translational modificationi

Can be acetylated.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP63072.

PTM databases

PhosphoSiteiP63072.

Expressioni

Tissue specificityi

Mostly expressed in thymus and intestine.1 Publication

Inductioni

Rapidly degraded by the proteasome. Cell-cycle regulation with highest expression during the S-phase (at protein level). Over expressed in hepatocytes by drug injury (e.g. DDC; diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate). Inducible by the proinflammatory cytokines tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg).3 Publications

Gene expression databases

BgeeiP63072.
CleanExiMM_UBD.
GenevestigatoriP63072.

Interactioni

Subunit structurei

Interact directly with the 26S proteasome. The interaction with NUB1L vie the N-terminal ubiquitin domain facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates its own degradation and that of its conjugates (By similarity). Interacts with the spindle checkpoint protein MAD2L1 during mitosis. Present in aggresomes of proteasome inhibited cells. Interacts with HDAC6 under proteasome impairment conditions. Forms a thioester with UBA6 in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg).By similarity1 Publication

Protein-protein interaction databases

BioGridi204900. 3 interactions.
MINTiMINT-1341042.
STRINGi10090.ENSMUSP00000035808.

Structurei

3D structure databases

ProteinModelPortaliP63072.
SMRiP63072. Positions 9-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 7876Ubiquitin-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 16074Ubiquitin-like 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ubiquitin-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG259502.
GeneTreeiENSGT00760000119340.
HOGENOMiHOG000134446.
HOVERGENiHBG101094.
InParanoidiP63072.
KOiK12157.
OMAiTCNGKKL.
OrthoDBiEOG75TMDT.
PhylomeDBiP63072.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 2 hits.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 2 hits.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 2 hits.
PROSITEiPS50053. UBIQUITIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63072-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASVRTCVVR SDQWRLMTFE TTENDKVKKI NEHIRSQTKV SVQDQILLLD
60 70 80 90 100
SKILKPHRKL SSYGIDKETT IHLTLKVVKP SDEELPLFLV ESKNEGQRHL
110 120 130 140 150
LRVRRSSSVA QVKEMIESVT SVIPKKQVVN CNGKKLEDGK IMADYNIKSG
160
SLLFLTTHCT GG
Length:162
Mass (Da):18,376
Last modified:September 13, 2004 - v1
Checksum:i6107D8604F96455F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314088 Genomic DNA. Translation: AAG27477.1.
AK008116 mRNA. Translation: BAB25471.1.
AK008552 mRNA. Translation: BAB25738.1.
CT010257 mRNA. Translation: CAJ18465.1.
AL078630 Genomic DNA. No translation available.
BC027627 mRNA. Translation: AAH27627.2.
BC036383 mRNA. Translation: AAH36383.1.
CCDSiCCDS28737.1.
RefSeqiNP_075626.1. NM_023137.3.
UniGeneiMm.140210.

Genome annotation databases

EnsembliENSMUST00000038844; ENSMUSP00000035808; ENSMUSG00000035186.
GeneIDi24108.
KEGGimmu:24108.
UCSCiuc008cmi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314088 Genomic DNA. Translation: AAG27477.1 .
AK008116 mRNA. Translation: BAB25471.1 .
AK008552 mRNA. Translation: BAB25738.1 .
CT010257 mRNA. Translation: CAJ18465.1 .
AL078630 Genomic DNA. No translation available.
BC027627 mRNA. Translation: AAH27627.2 .
BC036383 mRNA. Translation: AAH36383.1 .
CCDSi CCDS28737.1.
RefSeqi NP_075626.1. NM_023137.3.
UniGenei Mm.140210.

3D structure databases

ProteinModelPortali P63072.
SMRi P63072. Positions 9-162.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204900. 3 interactions.
MINTi MINT-1341042.
STRINGi 10090.ENSMUSP00000035808.

PTM databases

PhosphoSitei P63072.

Proteomic databases

PRIDEi P63072.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000038844 ; ENSMUSP00000035808 ; ENSMUSG00000035186 .
GeneIDi 24108.
KEGGi mmu:24108.
UCSCi uc008cmi.2. mouse.

Organism-specific databases

CTDi 10537.
MGIi MGI:1344410. Ubd.

Phylogenomic databases

eggNOGi NOG259502.
GeneTreei ENSGT00760000119340.
HOGENOMi HOG000134446.
HOVERGENi HBG101094.
InParanoidi P63072.
KOi K12157.
OMAi TCNGKKL.
OrthoDBi EOG75TMDT.
PhylomeDBi P63072.

Miscellaneous databases

ChiTaRSi Ubd. mouse.
NextBioi 304123.
PROi P63072.
SOURCEi Search...

Gene expression databases

Bgeei P63072.
CleanExi MM_UBD.
Genevestigatori P63072.

Family and domain databases

InterProi IPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00240. ubiquitin. 2 hits.
[Graphical view ]
PRINTSi PR00348. UBIQUITIN.
SMARTi SM00213. UBQ. 2 hits.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 2 hits.
PROSITEi PS50053. UBIQUITIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Disruption of the mouse Fat10 gene by gene targeting in the mouse embryonic stem cells."
    Yu X., Liu Y., Weissman S.M.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Small intestine.
  3. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
    Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  6. "The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis."
    Raasi S., Schmidtke G., Groettrup M.
    J. Biol. Chem. 276:35334-35343(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF 161-GLY-GLY-162.
  7. "FAT10, a ubiquitin-independent signal for proteasomal degradation."
    Hipp M.S., Kalveram B., Raasi S., Groettrup M., Schmidtke G.
    Mol. Cell. Biol. 25:3483-3491(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: FUNCTION.
  9. "FAT10/diubiquitin-like protein-deficient mice exhibit minimal phenotypic differences."
    Canaan A., Yu X., Booth C.J., Lian J., Lazar I., Gamfi S.L., Castille K., Kohya N., Nakayama Y., Liu Y.-C., Eynon E., Flavell R., Weissman S.M.
    Mol. Cell. Biol. 26:5180-5189(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY TNFA AND IFNG, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  10. "E1-L2 activates both ubiquitin and FAT10."
    Chiu Y.-H., Sun Q., Chen Z.J.
    Mol. Cell 27:1014-1023(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UBA6.
  11. "Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model of tumorigenesis."
    Oliva J., Bardag-Gorce F., French B.A., Li J., McPhaul L., Amidi F., Dedes J., Habibi A., Nguyen S., French S.W.
    Exp. Mol. Pathol. 84:102-112(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY DRUG INJURY.
  12. Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiUBD_MOUSE
AccessioniPrimary (citable) accession number: P63072
Secondary accession number(s): Q4FJZ5, Q9WV10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3