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P63057 (NOE3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Noelin-3
Alternative name(s):
Olfactomedin-3
Optimedin
Gene names
Name:Olfm3
Synonyms:Noe3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including OLFM3. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing. Homodimer. Interacts with MYOC. Ref.1 Ref.2

Subcellular location

Secreted. Cell junction › synapse Probable. Note: Isoform 2 is secreted more efficiently than isoform 1. Ref.2

Tissue specificity

Expressed in the brain (at protein level). Also expressed in the retina, mainly in the ganglion cell layer and in the amacrine cell subregion of the inner nuclear layer. Expressed at high levels in the epithelial cells of the posterior iris and the ciliary body and, at lower levels, in the trabecular meshwork. Isoform 2 preferentially expressed in retina and brain, while isoform 1 preferentially expressed in the tissues of the eye angle. Ref.1 Ref.2

Sequence similarities

Contains 1 olfactomedin-like domain.

Ontologies

Keywords
   Cellular componentCell junction
Secreted
Synapse
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processeye photoreceptor cell development

Inferred from direct assay Ref.1. Source: RGD

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Compara

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

extracellular space

Inferred from electronic annotation. Source: Compara

synapse

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P63057-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P63057-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: MSAPLLKLGAVLSTMAMISNWMSQTLPSLVGLNTTRLSAPDTL → MQARSSFLNLLLLSLLAGLDPSK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 478455Noelin-3
PRO_0000020082

Regions

Domain218 – 470253Olfactomedin-like
Coiled coil77 – 217141 Potential

Amino acid modifications

Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Disulfide bond219 ↔ 401 By similarity

Natural variations

Alternative sequence1 – 4343MSAPL…APDTL → MQARSSFLNLLLLSLLAGLD PSK in isoform 2.
VSP_011549

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (B) [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: ED4250E58213787D

FASTA47854,886
        10         20         30         40         50         60 
MSAPLLKLGA VLSTMAMISN WMSQTLPSLV GLNTTRLSAP DTLTQISPKE GWQVYSSAQD 

        70         80         90        100        110        120 
PDGRCICTVV APEQNLCSRD AKSRQLRQLL EKVQNMSQSI EVLNLRTQRD FQYVLKMETQ 

       130        140        150        160        170        180 
MKGLKAKFRQ IEDDRKTLMT KHFQELKEKM DELLPLIPVL EQYKTDAKLI TQFKEEIRNL 

       190        200        210        220        230        240 
SSVLTGIQEE IGAYDYEELH QRVLSLETRL RDCMKKLTCG KLMKITGPIT VKTSGTRFGA 

       250        260        270        280        290        300 
WMTDPLASEK NNRVWYMDSY TNNKIVREYK SIADFVSGAE SRTYNLPFKW AGTNHVVYNG 

       310        320        330        340        350        360 
SLYFNKYQSN IIIKYSFDLG RVLAQRSLEY AGFHNVYPYT WGGFSDIDLM ADEIGLWAVY 

       370        380        390        400        410        420 
ATNQNAGNIV ISQLNQDTLE VMKSWSTGYP KRSAGESFMI CGTLYVTNSH LTGAKVYYSY 

       430        440        450        460        470 
STKTSTYEYT DIPFHNQYFH ISMLDYNARD RALYAWNNGH QVLFNVTLFH IIKTEDDT

« Hide

Isoform 2 (A) [UniParc] [UniParc].

Checksum: 80371F2B171E58EF
Show »

FASTA45852,841

References

[1]"Optimedin: a novel olfactomedin-related protein that interacts with myocilin."
Torrado M., Trivedi R., Zinovieva R., Karavanova I., Tomarev S.I.
Hum. Mol. Genet. 11:1291-1301(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, INTERACTION WITH MYOC.
Strain: Wistar.
Tissue: Eye.
[2]"High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes."
Schwenk J., Harmel N., Brechet A., Zolles G., Berkefeld H., Muller C.S., Bildl W., Baehrens D., Huber B., Kulik A., Klocker N., Schulte U., Fakler B.
Neuron 74:621-633(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AMPAR COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF442822 mRNA. Translation: AAL87041.1.
AF442823 mRNA. Translation: AAL87042.1.
IPIIPI00206275.
IPI00337161.
RefSeqNP_665720.1. NM_145777.1.
UniGeneRn.27711.

3D structure databases

ProteinModelPortalP63057.
ModBaseSearch...

Proteomic databases

PaxDbP63057.
PRIDEP63057.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024243; ENSRNOP00000024243; ENSRNOG00000017969.
GeneID252920.
KEGGrno:252920.
UCSCRGD:628672. rat.

Organism-specific databases

CTD118427.
RGD628672. Olfm3.

Phylogenomic databases

eggNOGNOG323050.
GeneTreeENSGT00660000095208.
HOGENOMHOG000232069.
HOVERGENHBG006513.
InParanoidP63057.
OMAMKSWNTG.
OrthoDBEOG4R23TR.

Gene expression databases

ArrayExpressP63057.
GenevestigatorP63057.
GermOnlineENSRNOG00000017969. Rattus norvegicus.

Family and domain databases

InterProIPR022082. Noelin-1.
IPR003112. Olfac-like.
[Graphical view]
PfamPF12308. Noelin-1. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
SMARTSM00284. OLF. 1 hit.
[Graphical view]
PROSITEPS51132. OLF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624065.

Entry information

Entry nameNOE3_RAT
AccessionPrimary (citable) accession number: P63057
Secondary accession number(s): Q8BKV2 expand/collapse secondary AC list , Q8BLL6, Q8QZW0, Q8R4K3, Q8R4K4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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