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Protein

Vesicle-associated membrane protein 2

Gene

Vamp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19690160).By similarity1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: ParkinsonsUK-UCL
  • identical protein binding Source: IntAct
  • ion channel binding Source: UniProtKB
  • myosin binding Source: RGD
  • phospholipid binding Source: Ensembl
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI
  • syntaxin-1 binding Source: MGI

GO - Biological processi

  • cellular response to insulin stimulus Source: Ensembl
  • eosinophil degranulation Source: Ensembl
  • Golgi to plasma membrane protein transport Source: Ensembl
  • long-term synaptic potentiation Source: Ensembl
  • positive regulation of intracellular protein transport Source: Ensembl
  • protein complex assembly Source: RGD
  • protein transport Source: RGD
  • regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • regulation of exocytosis Source: UniProtKB
  • response to glucose Source: UniProtKB
  • synaptic vesicle exocytosis Source: Ensembl
  • vesicle fusion Source: GO_Central
  • vesicle-mediated transport Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-421837. Clathrin derived vesicle budding.
R-RNO-432720. Lysosome Vesicle Biogenesis.
R-RNO-432722. Golgi Associated Vesicle Biogenesis.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 2
Short name:
VAMP-2
Alternative name(s):
Synaptobrevin-2
Gene namesi
Name:Vamp2
Synonyms:Syb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3949. Vamp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9493CytoplasmicSequence analysisAdd
BLAST
Transmembranei95 – 11420Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini115 – 1162VesicularSequence analysis

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • clathrin-coated vesicle Source: ParkinsonsUK-UCL
  • extracellular exosome Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • intracellular organelle Source: RGD
  • membrane Source: ParkinsonsUK-UCL
  • neuron projection Source: RGD
  • neuron projection terminus Source: ParkinsonsUK-UCL
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: UniProtKB
  • secretory granule Source: UniProtKB
  • SNARE complex Source: ParkinsonsUK-UCL
  • storage vacuole Source: Ensembl
  • synaptic vesicle Source: ParkinsonsUK-UCL
  • synaptic vesicle membrane Source: ParkinsonsUK-UCL
  • synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: RGD
  • synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
  • synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: RGD
  • terminal bouton Source: ParkinsonsUK-UCL
  • trans-Golgi network Source: Ensembl
  • zymogen granule membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 116115Vesicle-associated membrane protein 2PRO_0000206726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Post-translational modificationi

Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP63045.
PRIDEiP63045.

PTM databases

iPTMnetiP63045.
PhosphoSiteiP63045.
SwissPalmiP63045.

Miscellaneous databases

PMAP-CutDBP63045.

Expressioni

Tissue specificityi

Nervous system specific. A higher level expression is seen in the brain as compared to the spinal cord.1 Publication

Gene expression databases

BgeeiENSRNOG00000006989.
ExpressionAtlasiP63045. baseline and differential.
GenevisibleiP63045. RN.

Interactioni

Subunit structurei

Interacts (via N-terminus) with KCNB1 (via N-terminus and C-terminus); stimulates the channel inactivation rate of KCNB1 (PubMed:19690160). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with BVES and STX4. Interacts with VAPA and VAPB. Interacts with WDFY2, PRKCZ and PRKCI (By similarity). Forms a complex with WDFY2 and PRKCZ (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-520880,EBI-520880
Prkd3D4A2292EBI-520880,EBI-1255458
Stx1aP328515EBI-520880,EBI-539720
Stx4Q088508EBI-520880,EBI-918243

GO - Molecular functioni

  • calcium-dependent protein binding Source: ParkinsonsUK-UCL
  • identical protein binding Source: IntAct
  • ion channel binding Source: UniProtKB
  • myosin binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI
  • syntaxin-1 binding Source: MGI

Protein-protein interaction databases

BioGridi246926. 2 interactions.
DIPiDIP-35503N.
IntActiP63045. 18 interactions.
MINTiMINT-1487471.
STRINGi10116.ENSRNOP00000054114.

Structurei

Secondary structure

1
116
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 284Combined sources
Helixi29 – 8860Combined sources
Helixi91 – 11525Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KILX-ray2.30A28-92[»]
1N7SX-ray1.45A28-88[»]
1SFCX-ray2.40A/E/I1-96[»]
2BU0model-A25-88[»]
2KOGNMR-A1-116[»]
2N1TNMR-A25-93[»]
3HD7X-ray3.40A/E30-116[»]
3IPDX-ray4.80A/E30-116[»]
3J96electron microscopy7.60K28-89[»]
3J97electron microscopy7.80K28-89[»]
3J98electron microscopy8.40K28-89[»]
3J99electron microscopy8.20K28-89[»]
5CCGX-ray3.50A/G28-89[»]
5CCHX-ray3.60A28-89[»]
5CCIX-ray4.10A28-89[»]
DisProtiDP00622.
ProteinModelPortaliP63045.
SMRiP63045. Positions 30-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63045.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 9161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63045.
KOiK13504.
OMAiFCAHRSA.
OrthoDBiEOG091G0MPE.
PhylomeDBiP63045.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV
60 70 80 90 100
DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG
110
VICAIILIII IVYFST
Length:116
Mass (Da):12,691
Last modified:January 23, 2007 - v2
Checksum:i4A0D0D56B5409D0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24105 mRNA. Translation: AAA42321.1.
BC074003 mRNA. Translation: AAH74003.1.
PIRiB34288.
RefSeqiNP_036795.1. NM_012663.2.
UniGeneiRn.12939.

Genome annotation databases

EnsembliENSRNOT00000057295; ENSRNOP00000054114; ENSRNOG00000006989.
GeneIDi24803.
KEGGirno:24803.
UCSCiRGD:3949. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24105 mRNA. Translation: AAA42321.1.
BC074003 mRNA. Translation: AAH74003.1.
PIRiB34288.
RefSeqiNP_036795.1. NM_012663.2.
UniGeneiRn.12939.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KILX-ray2.30A28-92[»]
1N7SX-ray1.45A28-88[»]
1SFCX-ray2.40A/E/I1-96[»]
2BU0model-A25-88[»]
2KOGNMR-A1-116[»]
2N1TNMR-A25-93[»]
3HD7X-ray3.40A/E30-116[»]
3IPDX-ray4.80A/E30-116[»]
3J96electron microscopy7.60K28-89[»]
3J97electron microscopy7.80K28-89[»]
3J98electron microscopy8.40K28-89[»]
3J99electron microscopy8.20K28-89[»]
5CCGX-ray3.50A/G28-89[»]
5CCHX-ray3.60A28-89[»]
5CCIX-ray4.10A28-89[»]
DisProtiDP00622.
ProteinModelPortaliP63045.
SMRiP63045. Positions 30-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246926. 2 interactions.
DIPiDIP-35503N.
IntActiP63045. 18 interactions.
MINTiMINT-1487471.
STRINGi10116.ENSRNOP00000054114.

PTM databases

iPTMnetiP63045.
PhosphoSiteiP63045.
SwissPalmiP63045.

Proteomic databases

PaxDbiP63045.
PRIDEiP63045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000057295; ENSRNOP00000054114; ENSRNOG00000006989.
GeneIDi24803.
KEGGirno:24803.
UCSCiRGD:3949. rat.

Organism-specific databases

CTDi6844.
RGDi3949. Vamp2.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63045.
KOiK13504.
OMAiFCAHRSA.
OrthoDBiEOG091G0MPE.
PhylomeDBiP63045.

Enzyme and pathway databases

ReactomeiR-RNO-181430. Norepinephrine Neurotransmitter Release Cycle.
R-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-264642. Acetylcholine Neurotransmitter Release Cycle.
R-RNO-421837. Clathrin derived vesicle budding.
R-RNO-432720. Lysosome Vesicle Biogenesis.
R-RNO-432722. Golgi Associated Vesicle Biogenesis.
R-RNO-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

EvolutionaryTraceiP63045.
PMAP-CutDBP63045.
PROiP63045.

Gene expression databases

BgeeiENSRNOG00000006989.
ExpressionAtlasiP63045. baseline and differential.
GenevisibleiP63045. RN.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP2_RAT
AccessioniPrimary (citable) accession number: P63045
Secondary accession number(s): Q64357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.