Reviewed, UniProtKB/Swiss-Prot P63045 (VAMP2_RAT)

Names and origin

Protein names

Recommended name:
    Vesicle-associated membrane protein 2
      Short name=VAMP-2
Alternative name(s):
    Synaptobrevin-2

Gene names

Name:	Vamp2
Synonyms:	Syb2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane By similarity.
Subunit structure	Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and VAPB By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction › synapse › synaptosome. Note: Neuronal synaptic vesicles.
Tissue specificity	Nervous system specific. A higher level expression is seen in the brain as compared to the spinal cord. Ref.1
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	protein complex assembly Inferred from direct assay. Source: RGD protein transport Inferred from mutant phenotype. Source: RGD regulation of exocytosis Traceable author statement. Source: UniProtKB response to glucose stimulus Inferred from direct assay. Source: UniProtKB vesicle-mediated transport Inferred from mutant phenotype. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: RGD secretory granule Inferred from direct assay. Source: UniProtKB synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptobrevin 2-SNAP-25-syntaxin-1a complex Inferred from direct assay. Source: MGI synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Inferred from direct assay. Source: MGI synaptosome Inferred from direct assay. Source: RGD
Molecular function	myosin binding Inferred from physical interaction. Source: RGD protein complex binding Inferred from physical interaction. Source: RGD syntaxin-1 binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rabac1	O35394	1	EBI-520880,EBI-2028510
Stx1a	P32851	2	EBI-520880,EBI-539720

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 116

115

Vesicle-associated membrane protein 2

PRO_0000206726

Regions

Topological domain

2 – 94

93

Cytoplasmic Potential

Transmembrane

95 – 114

20

Anchor for type IV membrane protein Potential

Topological domain

115 – 116

2

Vesicular Potential

Domain

31 – 91

61

v-SNARE coiled-coil homology

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

75

1

Phosphoserine By similarity

Secondary structure

1

.

.

.

116

Helix Strand Turn

Details...

Helix

29 – 88

60

Sequences

	Sequence		Length	Mass (Da)
	P63045-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 4A0D0D56B5409D0A	FASTA	116	12,691
	10 20 30 40 50 60 MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL 70 80 90 100 110 SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." Elferink L.A., Trimble W.S., Scheller R.H. J. Biol. Chem. 264:11061-11064(1989) [PubMed: 2472388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	Lubec G., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 32-47 AND 60-83, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
[4]	"Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." Sutton R.B., Fasshauer D., Jahn R., Brunger A.T. Nature 395:347-353(1998) [PubMed: 9759724] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-97 IN COMPLEX WITH STX1A AND SNAP25.
[5]	"Three-dimensional structure of the complexin/SNARE complex." Chen X., Tomchick D.R., Kovrigin E., Arac D., Machius M., Suedhof T.C., Rizo J. Neuron 33:397-409(2002) [PubMed: 11832227] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 30-94 IN COMPLEX WITH STX1A; CPLX1 AND SNAP25, STRUCTURE BY NMR.
[6]	"High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex." Ernst J.A., Brunger A.T. J. Biol. Chem. 278:8630-8636(2003) [PubMed: 12496247] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 29-90 IN COMPLEX WITH STX1A AND SNAP25.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M24105 mRNA. Translation: AAA42321.1.
BC074003 mRNA. Translation: AAH74003.1.

IPI

IPI00781701.

PIR

B34288.

RefSeq

NP_036795.1.

UniGene

Rn.12939

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KIL	X-ray	2.30	A	28-92	[»]
1N7S	X-ray	1.45	A	28-89	[»]
1SFC	X-ray	2.40	A/E/I	1-96	[»]
2BU0	model	-	A	25-88	[»]
2KOG	NMR	-	A	1-116	[»]
3HD7	X-ray	3.40	A/E	30-116	[»]
3IPD	X-ray	4.80	A/E	30-116	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P63045. 14 interactions.

STRING

P63045.

PTM databases

PhosphoSite

P63045.

Proteomic databases

PRIDE

P63045.

Genome annotation databases

Ensembl

ENSRNOT00000057295; ENSRNOP00000054114; ENSRNOG00000006989; Rattus norvegicus. [Genome view]

GeneID

24803.

KEGG

rno:24803.

UCSC

NM_012663. rat.

Organism-specific databases

CTD

24803.

RGD

3949. Vamp2.

Phylogenomic databases

eggNOG

veNOG21495.

HOVERGEN

P63045.

InParanoid

P63045.

Gene expression databases

ArrayExpress

P63045.

Genevestigator

P63045.

GermOnline

ENSRNOG00000006989. Rattus norvegicus.

Family and domain databases

InterPro

IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin_met/fun.
[Graphical view]

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PIRSF

PIRSF005409. Synaptobrevin_euk. 1 hit.

PRINTS

PR00219. SYNAPTOBREVN.

PROSITE

PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

604468.

PMAP-CutDB

P63045.

Entry information

Entry name

VAMP2_RAT

Accession

Primary (citable) accession number: P63045
Secondary accession number(s): Q64357

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)