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Protein

Vesicle-associated membrane protein 2

Gene

Vamp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19690160).By similarity1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: ParkinsonsUK-UCL
  • calmodulin binding Source: Ensembl
  • identical protein binding Source: IntAct
  • ion channel binding Source: UniProtKB
  • phospholipid binding Source: Ensembl
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI
  • syntaxin-1 binding Source: MGI

GO - Biological processi

  • cellular response to insulin stimulus Source: Ensembl
  • exocytic insertion of neurotransmitter receptor to postsynaptic membrane Source: SynGO
  • Golgi to plasma membrane protein transport Source: Ensembl
  • long-term synaptic potentiation Source: Ensembl
  • positive regulation of intracellular protein transport Source: Ensembl
  • protein-containing complex assembly Source: RGD
  • protein transport Source: RGD
  • regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • regulation of exocytosis Source: RGD
  • regulation of synaptic vesicle recycling Source: RGD
  • response to glucose Source: UniProtKB
  • SNARE complex assembly Source: CAFA
  • synaptic vesicle exocytosis Source: Ensembl
  • vesicle-mediated transport Source: RGD

Enzyme and pathway databases

ReactomeiR-RNO-181429 Serotonin Neurotransmitter Release Cycle
R-RNO-181430 Norepinephrine Neurotransmitter Release Cycle
R-RNO-210500 Glutamate Neurotransmitter Release Cycle
R-RNO-212676 Dopamine Neurotransmitter Release Cycle
R-RNO-264642 Acetylcholine Neurotransmitter Release Cycle
R-RNO-421837 Clathrin derived vesicle budding
R-RNO-432720 Lysosome Vesicle Biogenesis
R-RNO-432722 Golgi Associated Vesicle Biogenesis
R-RNO-449836 Other interleukin signaling
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis
R-RNO-888590 GABA synthesis, release, reuptake and degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 2
Short name:
VAMP-2
Alternative name(s):
Synaptobrevin-2
Gene namesi
Name:Vamp2
Synonyms:Syb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3949 Vamp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 94CytoplasmicSequence analysisAdd BLAST93
Transmembranei95 – 114Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST20
Topological domaini115 – 116VesicularSequence analysis2

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002067262 – 116Vesicle-associated membrane protein 2Add BLAST115

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP63045
PRIDEiP63045

PTM databases

iPTMnetiP63045
PhosphoSitePlusiP63045
SwissPalmiP63045

Miscellaneous databases

PMAP-CutDBiP63045

Expressioni

Tissue specificityi

Nervous system specific. A higher level expression is seen in the brain as compared to the spinal cord.1 Publication

Gene expression databases

BgeeiENSRNOG00000006989
GenevisibleiP63045 RN

Interactioni

Subunit structurei

Interacts (via N-terminus) with KCNB1 (via N-terminus and C-terminus); stimulates the channel inactivation rate of KCNB1 (PubMed:19690160). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with BVES and STX4. Interacts with VAPA and VAPB. Interacts with WDFY2, PRKCZ and PRKCI (By similarity). Forms a complex with WDFY2 and PRKCZ (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • calcium-dependent protein binding Source: ParkinsonsUK-UCL
  • calmodulin binding Source: Ensembl
  • identical protein binding Source: IntAct
  • ion channel binding Source: UniProtKB
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI
  • syntaxin-1 binding Source: MGI

Protein-protein interaction databases

BioGridi246926, 2 interactors
CORUMiP63045
DIPiDIP-35503N
IntActiP63045, 22 interactors
MINTiP63045
STRINGi10116.ENSRNOP00000054114

Structurei

Secondary structure

1116
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 28Combined sources4
Helixi29 – 88Combined sources60
Helixi91 – 115Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KILX-ray2.30A28-92[»]
1N7SX-ray1.45A28-88[»]
1SFCX-ray2.40A/E/I1-96[»]
2BU0model-A25-88[»]
2KOGNMR-A1-116[»]
2N1TNMR-A25-93[»]
3HD7X-ray3.40A/E30-116[»]
3IPDX-ray4.80A/E30-116[»]
3J96electron microscopy7.60K28-89[»]
3J97electron microscopy7.80K28-89[»]
3J98electron microscopy8.40K28-89[»]
3J99electron microscopy8.20K28-89[»]
5CCGX-ray3.50A/G28-89[»]
5CCHX-ray3.60A28-89[»]
5CCIX-ray4.10A28-89[»]
5W5CX-ray1.85A28-66[»]
5W5DX-ray2.50A28-66[»]
DisProtiDP00622
ProteinModelPortaliP63045
SMRiP63045
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63045

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 91v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiP63045
KOiK13504
OMAiKMMIMMA
OrthoDBiEOG091G0MPE
PhylomeDBiP63045

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
IPR028717 VAMP2
PANTHERiPTHR21136:SF91 PTHR21136:SF91, 1 hit
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV
60 70 80 90 100
DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG
110
VICAIILIII IVYFST
Length:116
Mass (Da):12,691
Last modified:January 23, 2007 - v2
Checksum:i4A0D0D56B5409D0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24105 mRNA Translation: AAA42321.1
BC074003 mRNA Translation: AAH74003.1
PIRiB34288
RefSeqiNP_036795.1, NM_012663.2
UniGeneiRn.12939

Genome annotation databases

EnsembliENSRNOT00000057295; ENSRNOP00000054114; ENSRNOG00000006989
GeneIDi24803
KEGGirno:24803
UCSCiRGD:3949 rat

Similar proteinsi

Entry informationi

Entry nameiVAMP2_RAT
AccessioniPrimary (citable) accession number: P63045
Secondary accession number(s): Q64357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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