Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P63044 (VAMP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle-associated membrane protein 2

Short name=VAMP-2
Alternative name(s):
Synaptobrevin-2
Gene names
Name:Vamp2
Synonyms:Syb2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length116 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Ref.1

Subunit structure

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and VAPB By similarity. Interacts with BVES and STX4. Ref.4 Ref.6

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass type IV membrane protein. Cell junctionsynapsesynaptosome. Note: Neuronal synaptic vesicles.

Sequence similarities

Belongs to the synaptobrevin family.

Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   DomainCoiled coil
Transmembrane
Transmembrane helix
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to plasma membrane protein transport

Inferred from mutant phenotype PubMed 20582536. Source: MGI

calcium ion-dependent exocytosis

Inferred from direct assay PubMed 12145198. Source: MGI

cellular response to insulin stimulus

Inferred from direct assay PubMed 18227281. Source: MGI

long-term synaptic potentiation

Inferred from mutant phenotype PubMed 23395379. Source: MGI

membrane fusion

Inferred from mutant phenotype PubMed 15920476. Source: MGI

positive regulation of intracellular protein transport

Inferred from mutant phenotype PubMed 18227281. Source: MGI

protein complex assembly

Inferred from electronic annotation. Source: Ensembl

regulation of exocytosis

Inferred from direct assay PubMed 12145198. Source: MGI

regulation of vesicle-mediated transport

Inferred from mutant phenotype PubMed 18227281. Source: MGI

response to glucose

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle exocytosis

Inferred from mutant phenotype PubMed 11691998. Source: MGI

vesicle fusion

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentSNARE complex

Inferred from sequence or structural similarity. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasmic vesicle

Inferred from direct assay PubMed 18505797. Source: MGI

cytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from sequence orthology PubMed 22375059. Source: MGI

neuron projection

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from direct assay PubMed 18827011. Source: UniProtKB

plasma membrane

Inferred from direct assay PubMed 18505797. Source: MGI

secretory granule

Inferred from direct assay PubMed 12145198. Source: MGI

secretory granule membrane

Traceable author statement. Source: Reactome

synapse

Inferred from direct assay PubMed 18706977PubMed 20943658PubMed 23791195. Source: MGI

synaptic vesicle

Inferred from direct assay PubMed 15145078. Source: MGI

synaptic vesicle membrane

Inferred from direct assay PubMed 12192047PubMed 15071120. Source: MGI

synaptobrevin 2-SNAP-25-syntaxin-1a complex

Inferred from direct assay PubMed 21040848. Source: MGI

synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex

Inferred from sequence orthology PubMed 7553862. Source: MGI

synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex

Inferred from electronic annotation. Source: Ensembl

trans-Golgi network

Inferred from direct assay PubMed 18827011. Source: UniProtKB

zymogen granule membrane

Inferred from direct assay PubMed 12192047. Source: MGI

   Molecular_functionSNAP receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

SNARE binding

Inferred from direct assay PubMed 12145198. Source: MGI

calmodulin binding

Inferred from direct assay PubMed 12145198. Source: MGI

phospholipid binding

Inferred from direct assay PubMed 12145198. Source: MGI

protein binding

Inferred from physical interaction PubMed 18827011Ref.6. Source: UniProtKB

syntaxin binding

Inferred from physical interaction Ref.4. Source: UniProtKB

syntaxin-1 binding

Inferred from sequence orthology PubMed 7553862. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Cdc42P607662EBI-521920,EBI-81763
Snap25P608795EBI-521920,EBI-445270

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 116115Vesicle-associated membrane protein 2
PRO_0000206725

Regions

Topological domain2 – 9493Cytoplasmic Potential
Transmembrane95 – 11420Helical; Anchor for type IV membrane protein; Potential
Topological domain115 – 1162Vesicular Potential
Domain31 – 9161v-SNARE coiled-coil homology

Amino acid modifications

Modified residue21N-acetylserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P63044 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 4A0D0D56B5409D0A

FASTA11612,691
        10         20         30         40         50         60 
MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL 

        70         80         90        100        110 
SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFST 

« Hide

References

« Hide 'large scale' references
[1]"Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes."
Martin L.B., Shewan A., Millar C.A., Gould G.W., James D.E.
J. Biol. Chem. 273:1444-1452(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
Ke B., Oh E., Thurmond D.C.
J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STX4.
[5]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 31-47 AND 60-83, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[6]"Identification of a novel Bves function: regulation of vesicular transport."
Hager H.A., Roberts R.J., Cross E.E., Proux-Gillardeaux V., Bader D.M.
EMBO J. 29:532-545(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BVES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60150 mRNA. Translation: AAB03463.1.
AK090178 mRNA. Translation: BAC41125.1.
BC055105 mRNA. Translation: AAH55105.1.
CCDSCCDS24881.1.
RefSeqNP_033523.1. NM_009497.3.
UniGeneMm.28643.

3D structure databases

ProteinModelPortalP63044.
SMRP63044. Positions 30-116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204495. 8 interactions.
DIPDIP-29065N.
IntActP63044. 24 interactions.
MINTMINT-2411573.

PTM databases

PhosphoSiteP63044.

Proteomic databases

PaxDbP63044.
PRIDEP63044.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021273; ENSMUSP00000021273; ENSMUSG00000020894.
GeneID22318.
KEGGmmu:22318.
UCSCuc007jpe.1. mouse.

Organism-specific databases

CTD6844.
MGIMGI:1313277. Vamp2.

Phylogenomic databases

eggNOGNOG266115.
HOGENOMHOG000042711.
HOVERGENHBG006675.
KOK13504.
OrthoDBEOG7MSMRJ.
PhylomeDBP63044.
TreeFamTF313666.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.
REACT_188937. Metabolism.
REACT_209837. Membrane Trafficking.

Gene expression databases

ArrayExpressP63044.
BgeeP63044.
CleanExMM_VAMP2.
GenevestigatorP63044.

Family and domain databases

InterProIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP_met/fun.
[Graphical view]
PfamPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSPR00219. SYNAPTOBREVN.
PROSITEPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSVAMP2. mouse.
NextBio302529.
PROP63044.
SOURCESearch...

Entry information

Entry nameVAMP2_MOUSE
AccessionPrimary (citable) accession number: P63044
Secondary accession number(s): Q64357
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot