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P63044

- VAMP2_MOUSE

UniProt

P63044 - VAMP2_MOUSE

Protein

Vesicle-associated membrane protein 2

Gene

Vamp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.1 Publication

    GO - Molecular functioni

    1. calmodulin binding Source: MGI
    2. phospholipid binding Source: MGI
    3. protein binding Source: UniProtKB
    4. SNARE binding Source: MGI
    5. syntaxin-1 binding Source: MGI
    6. syntaxin binding Source: UniProtKB

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: MGI
    2. cellular response to insulin stimulus Source: MGI
    3. Golgi to plasma membrane protein transport Source: MGI
    4. long-term synaptic potentiation Source: MGI
    5. membrane fusion Source: MGI
    6. positive regulation of intracellular protein transport Source: MGI
    7. protein complex assembly Source: Ensembl
    8. regulation of exocytosis Source: MGI
    9. regulation of vesicle-mediated transport Source: MGI
    10. response to glucose Source: Ensembl
    11. synaptic vesicle exocytosis Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-associated membrane protein 2
    Short name:
    VAMP-2
    Alternative name(s):
    Synaptobrevin-2
    Gene namesi
    Name:Vamp2
    Synonyms:Syb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1313277. Vamp2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasmic vesicle Source: MGI
    3. cytoplasmic vesicle membrane Source: Reactome
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane Source: MGI
    6. neuron projection Source: UniProtKB-SubCell
    7. perinuclear region of cytoplasm Source: UniProtKB
    8. plasma membrane Source: MGI
    9. secretory granule Source: MGI
    10. secretory granule membrane Source: Reactome
    11. SNARE complex Source: UniProtKB
    12. synapse Source: MGI
    13. synaptic vesicle Source: MGI
    14. synaptic vesicle membrane Source: MGI
    15. synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
    16. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
    17. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl
    18. trans-Golgi network Source: UniProtKB
    19. zymogen granule membrane Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 116115Vesicle-associated membrane protein 2PRO_0000206725Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP63044.
    PRIDEiP63044.

    PTM databases

    PhosphoSiteiP63044.

    Expressioni

    Gene expression databases

    ArrayExpressiP63044.
    BgeeiP63044.
    CleanExiMM_VAMP2.
    GenevestigatoriP63044.

    Interactioni

    Subunit structurei

    Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and VAPB By similarity. Interacts with BVES and STX4.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cdc42P607662EBI-521920,EBI-81763
    Snap25P608795EBI-521920,EBI-445270

    Protein-protein interaction databases

    BioGridi204495. 8 interactions.
    DIPiDIP-29065N.
    IntActiP63044. 24 interactions.
    MINTiMINT-2411573.

    Structurei

    3D structure databases

    ProteinModelPortaliP63044.
    SMRiP63044. Positions 30-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 9493CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini115 – 1162VesicularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei95 – 11420Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 9161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the synaptobrevin family.Curated
    Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG266115.
    HOGENOMiHOG000042711.
    HOVERGENiHBG006675.
    KOiK13504.
    OrthoDBiEOG7MSMRJ.
    PhylomeDBiP63044.
    TreeFamiTF313666.

    Family and domain databases

    InterProiIPR001388. Synaptobrevin.
    IPR016444. Synaptobrevin/VAMP_met/fun.
    [Graphical view]
    PfamiPF00957. Synaptobrevin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
    PRINTSiPR00219. SYNAPTOBREVN.
    PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63044-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV    50
    DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG 100
    VICAIILIII IVYFST 116
    Length:116
    Mass (Da):12,691
    Last modified:January 23, 2007 - v2
    Checksum:i4A0D0D56B5409D0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60150 mRNA. Translation: AAB03463.1.
    AK090178 mRNA. Translation: BAC41125.1.
    BC055105 mRNA. Translation: AAH55105.1.
    CCDSiCCDS24881.1.
    RefSeqiNP_033523.1. NM_009497.3.
    UniGeneiMm.28643.

    Genome annotation databases

    EnsembliENSMUST00000021273; ENSMUSP00000021273; ENSMUSG00000020894.
    GeneIDi22318.
    KEGGimmu:22318.
    UCSCiuc007jpe.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60150 mRNA. Translation: AAB03463.1 .
    AK090178 mRNA. Translation: BAC41125.1 .
    BC055105 mRNA. Translation: AAH55105.1 .
    CCDSi CCDS24881.1.
    RefSeqi NP_033523.1. NM_009497.3.
    UniGenei Mm.28643.

    3D structure databases

    ProteinModelPortali P63044.
    SMRi P63044. Positions 30-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204495. 8 interactions.
    DIPi DIP-29065N.
    IntActi P63044. 24 interactions.
    MINTi MINT-2411573.

    PTM databases

    PhosphoSitei P63044.

    Proteomic databases

    PaxDbi P63044.
    PRIDEi P63044.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021273 ; ENSMUSP00000021273 ; ENSMUSG00000020894 .
    GeneIDi 22318.
    KEGGi mmu:22318.
    UCSCi uc007jpe.1. mouse.

    Organism-specific databases

    CTDi 6844.
    MGIi MGI:1313277. Vamp2.

    Phylogenomic databases

    eggNOGi NOG266115.
    HOGENOMi HOG000042711.
    HOVERGENi HBG006675.
    KOi K13504.
    OrthoDBi EOG7MSMRJ.
    PhylomeDBi P63044.
    TreeFami TF313666.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Miscellaneous databases

    ChiTaRSi VAMP2. mouse.
    NextBioi 302529.
    PROi P63044.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63044.
    Bgeei P63044.
    CleanExi MM_VAMP2.
    Genevestigatori P63044.

    Family and domain databases

    InterProi IPR001388. Synaptobrevin.
    IPR016444. Synaptobrevin/VAMP_met/fun.
    [Graphical view ]
    Pfami PF00957. Synaptobrevin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005409. Synaptobrevin_euk. 1 hit.
    PRINTSi PR00219. SYNAPTOBREVN.
    PROSITEi PS00417. SYNAPTOBREVIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes."
      Martin L.B., Shewan A., Millar C.A., Gould G.W., James D.E.
      J. Biol. Chem. 273:1444-1452(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
      Ke B., Oh E., Thurmond D.C.
      J. Biol. Chem. 282:21786-21797(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STX4.
    5. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 31-47 AND 60-83, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Identification of a novel Bves function: regulation of vesicular transport."
      Hager H.A., Roberts R.J., Cross E.E., Proux-Gillardeaux V., Bader D.M.
      EMBO J. 29:532-545(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BVES.

    Entry informationi

    Entry nameiVAMP2_MOUSE
    AccessioniPrimary (citable) accession number: P63044
    Secondary accession number(s): Q64357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3