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Protein

Vesicle-associated membrane protein 2

Gene

Vamp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane.1 Publication

GO - Molecular functioni

  1. calcium-dependent protein binding Source: ParkinsonsUK-UCL
  2. calmodulin binding Source: MGI
  3. phospholipid binding Source: MGI
  4. SNAP receptor activity Source: GO_Central
  5. SNARE binding Source: MGI
  6. syntaxin-1 binding Source: MGI
  7. syntaxin binding Source: UniProtKB

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: MGI
  2. cellular response to insulin stimulus Source: MGI
  3. eosinophil degranulation Source: MGI
  4. Golgi to plasma membrane protein transport Source: MGI
  5. long-term synaptic potentiation Source: MGI
  6. membrane fusion Source: MGI
  7. positive regulation of intracellular protein transport Source: MGI
  8. protein complex assembly Source: Ensembl
  9. regulation of calcium ion-dependent exocytosis Source: ParkinsonsUK-UCL
  10. regulation of exocytosis Source: MGI
  11. regulation of vesicle-mediated transport Source: MGI
  12. response to glucose Source: Ensembl
  13. synaptic vesicle exocytosis Source: MGI
  14. vesicle fusion Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_281668. Acetylcholine Neurotransmitter Release Cycle.
REACT_287745. Glutamate Neurotransmitter Release Cycle.
REACT_302653. Translocation of GLUT4 to the plasma membrane.
REACT_303468. Lysosome Vesicle Biogenesis.
REACT_306614. Regulation of insulin secretion.
REACT_319523. GABA synthesis, release, reuptake and degradation.
REACT_325189. Norepinephrine Neurotransmitter Release Cycle.
REACT_342454. Golgi Associated Vesicle Biogenesis.
REACT_347655. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 2
Short name:
VAMP-2
Alternative name(s):
Synaptobrevin-2
Gene namesi
Name:Vamp2
Synonyms:Syb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1313277. Vamp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9493CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei95 – 11420Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini115 – 1162VesicularSequence Analysis

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. clathrin-coated vesicle Source: ParkinsonsUK-UCL
  3. cytoplasmic vesicle Source: MGI
  4. cytoplasmic vesicle membrane Source: Reactome
  5. extracellular vesicular exosome Source: MGI
  6. integral component of membrane Source: UniProtKB-KW
  7. membrane Source: ParkinsonsUK-UCL
  8. neuron projection terminus Source: Ensembl
  9. perinuclear region of cytoplasm Source: UniProtKB
  10. plasma membrane Source: MGI
  11. secretory granule Source: MGI
  12. secretory granule membrane Source: Reactome
  13. SNARE complex Source: UniProtKB
  14. synapse Source: MGI
  15. synaptic vesicle Source: MGI
  16. synaptic vesicle membrane Source: MGI
  17. synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
  18. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
  19. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl
  20. trans-Golgi network Source: UniProtKB
  21. zymogen granule membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 116115Vesicle-associated membrane protein 2PRO_0000206725Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP63044.
PaxDbiP63044.
PRIDEiP63044.

PTM databases

PhosphoSiteiP63044.

Expressioni

Gene expression databases

BgeeiP63044.
CleanExiMM_VAMP2.
ExpressionAtlasiP63044. baseline and differential.
GenevestigatoriP63044.

Interactioni

Subunit structurei

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and VAPB (By similarity). Interacts with BVES and STX4.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdc42P607662EBI-521920,EBI-81763
Snap23O090442EBI-521920,EBI-1812522
Snap25P608795EBI-521920,EBI-445270

Protein-protein interaction databases

BioGridi204495. 22 interactions.
DIPiDIP-29065N.
IntActiP63044. 26 interactions.
MINTiMINT-2411573.

Structurei

3D structure databases

ProteinModelPortaliP63044.
SMRiP63044. Positions 30-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 9161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG266115.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63044.
KOiK13504.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP63044.
TreeFamiTF313666.

Family and domain databases

InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV
60 70 80 90 100
DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG
110
VICAIILIII IVYFST
Length:116
Mass (Da):12,691
Last modified:January 22, 2007 - v2
Checksum:i4A0D0D56B5409D0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60150 mRNA. Translation: AAB03463.1.
AK090178 mRNA. Translation: BAC41125.1.
BC055105 mRNA. Translation: AAH55105.1.
CCDSiCCDS24881.1.
RefSeqiNP_033523.1. NM_009497.3.
UniGeneiMm.28643.

Genome annotation databases

EnsembliENSMUST00000021273; ENSMUSP00000021273; ENSMUSG00000020894.
GeneIDi22318.
KEGGimmu:22318.
UCSCiuc007jpe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60150 mRNA. Translation: AAB03463.1.
AK090178 mRNA. Translation: BAC41125.1.
BC055105 mRNA. Translation: AAH55105.1.
CCDSiCCDS24881.1.
RefSeqiNP_033523.1. NM_009497.3.
UniGeneiMm.28643.

3D structure databases

ProteinModelPortaliP63044.
SMRiP63044. Positions 30-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204495. 22 interactions.
DIPiDIP-29065N.
IntActiP63044. 26 interactions.
MINTiMINT-2411573.

PTM databases

PhosphoSiteiP63044.

Proteomic databases

MaxQBiP63044.
PaxDbiP63044.
PRIDEiP63044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021273; ENSMUSP00000021273; ENSMUSG00000020894.
GeneIDi22318.
KEGGimmu:22318.
UCSCiuc007jpe.1. mouse.

Organism-specific databases

CTDi6844.
MGIiMGI:1313277. Vamp2.

Phylogenomic databases

eggNOGiNOG266115.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63044.
KOiK13504.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP63044.
TreeFamiTF313666.

Enzyme and pathway databases

ReactomeiREACT_281668. Acetylcholine Neurotransmitter Release Cycle.
REACT_287745. Glutamate Neurotransmitter Release Cycle.
REACT_302653. Translocation of GLUT4 to the plasma membrane.
REACT_303468. Lysosome Vesicle Biogenesis.
REACT_306614. Regulation of insulin secretion.
REACT_319523. GABA synthesis, release, reuptake and degradation.
REACT_325189. Norepinephrine Neurotransmitter Release Cycle.
REACT_342454. Golgi Associated Vesicle Biogenesis.
REACT_347655. Clathrin derived vesicle budding.

Miscellaneous databases

ChiTaRSiVamp2. mouse.
NextBioi302529.
PROiP63044.
SOURCEiSearch...

Gene expression databases

BgeeiP63044.
CleanExiMM_VAMP2.
ExpressionAtlasiP63044. baseline and differential.
GenevestigatoriP63044.

Family and domain databases

InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes."
    Martin L.B., Shewan A., Millar C.A., Gould G.W., James D.E.
    J. Biol. Chem. 273:1444-1452(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Doc2beta is a novel Munc18c-interacting partner and positive effector of syntaxin 4-mediated exocytosis."
    Ke B., Oh E., Thurmond D.C.
    J. Biol. Chem. 282:21786-21797(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STX4.
  5. Lubec G., Kang S.U.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 31-47 AND 60-83, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Identification of a novel Bves function: regulation of vesicular transport."
    Hager H.A., Roberts R.J., Cross E.E., Proux-Gillardeaux V., Bader D.M.
    EMBO J. 29:532-545(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BVES.

Entry informationi

Entry nameiVAMP2_MOUSE
AccessioniPrimary (citable) accession number: P63044
Secondary accession number(s): Q64357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2004
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.