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Protein

Complexin-1

Gene

Cplx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed:23345244). Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed:23345244). Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior (PubMed:15126625, PubMed:16000319).3 Publications

GO - Molecular functioni

  • neurotransmitter transporter activity Source: UniProtKB
  • syntaxin-1 binding Source: MGI

GO - Biological processi

  • insulin secretion Source: UniProtKB
  • synaptic vesicle exocytosis Source: UniProtKB

Keywordsi

Biological processExocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Complexin-1
Alternative name(s):
921-S
Complexin I
Short name:
CPX I
Synaphin-2
Gene namesi
Name:Cplx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:104727 Cplx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice have no obvious brain abnormality, but suffer from severe ataxia with dystonia starting from P7. Adult mice lacking Cplx1 are not capable of coordinated running or swimming and exhibit pronounced resting tremor. They also fail to habituate to confinement and have reduced exploration abilities in open field.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001448711 – 134Complexin-1Add BLAST134

Proteomic databases

MaxQBiP63040
PaxDbiP63040
PeptideAtlasiP63040
PRIDEiP63040

PTM databases

iPTMnetiP63040
PhosphoSitePlusiP63040

Expressioni

Tissue specificityi

Nervous system, and pancreatic islet cells. Present in many brain regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level).3 Publications

Developmental stagei

In the brain, expression starts at P6 and increases to reach a plateau at P20.1 Publication

Gene expression databases

BgeeiENSMUSG00000033615
CleanExiMM_CPLX1
ExpressionAtlasiP63040 baseline and differential
GenevisibleiP63040 MM

Interactioni

Subunit structurei

Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP63040
IntActiP63040, 1 interactor
MINTiP63040
STRINGi10090.ENSMUSP00000038502

Structurei

3D structure databases

ProteinModelPortaliP63040
SMRiP63040
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 70Interaction with the SNARE complexBy similarityAdd BLAST23

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili29 – 64Sequence analysisAdd BLAST36

Sequence similaritiesi

Belongs to the complexin/synaphin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IWGS Eukaryota
ENOG4111MX0 LUCA
GeneTreeiENSGT00490000043416
HOGENOMiHOG000017716
HOVERGENiHBG062792
InParanoidiP63040
KOiK15294
OMAiTVMKFIP
PhylomeDBiP63040
TreeFamiTF315172

Family and domain databases

InterProiView protein in InterPro
IPR008849 Synaphin
PANTHERiPTHR16705 PTHR16705, 1 hit
PfamiView protein in Pfam
PF05835 Synaphin, 1 hit

Sequencei

Sequence statusi: Complete.

P63040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA
60 70 80 90 100
KYAKMEAERE VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA
110 120 130
IPPGCGDEPE EEDESILDTV IKYLPGPLQD MFKK
Length:134
Mass (Da):15,122
Last modified:August 31, 2004 - v1
Checksum:iBA1B6F074923A3A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84A → V in AAH14803 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38614 mRNA Translation: BAA07605.1
AK134820 mRNA Translation: BAE22299.1
AK158166 mRNA Translation: BAE34390.1
AK159026 mRNA Translation: BAE34774.1
BC014803 mRNA Translation: AAH14803.1
CCDSiCCDS39206.1
PIRiS66294
RefSeqiNP_031782.3, NM_007756.3
UniGeneiMm.5195

Genome annotation databases

EnsembliENSMUST00000046892; ENSMUSP00000038502; ENSMUSG00000033615
GeneIDi12889
KEGGimmu:12889
UCSCiuc008yof.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCPLX1_MOUSE
AccessioniPrimary (citable) accession number: P63040
Secondary accession number(s): O09057
, O09142, Q3UYB3, Q64276, Q91WJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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