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P63040

- CPLX1_MOUSE

UniProt

P63040 - CPLX1_MOUSE

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Protein

Complexin-1

Gene

Cplx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Positively regulates a late step in synaptic vesicle exocytosis. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.2 Publications

GO - Molecular functioni

  1. neurotransmitter transporter activity Source: UniProtKB
  2. syntaxin-1 binding Source: MGI

GO - Biological processi

  1. insulin secretion Source: UniProtKB
  2. synaptic vesicle exocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.
REACT_229514. Glutamate Neurotransmitter Release Cycle.
REACT_242222. Norepinephrine Neurotransmitter Release Cycle.
REACT_245631. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Complexin-1
Alternative name(s):
921-S
Complexin I
Short name:
CPX I
Synaphin-2
Gene namesi
Name:Cplx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:104727. Cplx1.

Subcellular locationi

Cytoplasmcytosol 2 Publications
Note: Enriched at synaptic-releasing sites in mature neurons.

GO - Cellular componenti

  1. dendrite Source: MGI
  2. neuronal cell body Source: MGI
  3. SNARE complex Source: UniProtKB
  4. synapse Source: MGI
  5. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice have no obvious brain abnormality, but suffer from severe ataxia with dystonia starting from P7. Adult mice lacking Cplx1 are not capable of coordinated running or swimming and exhibit pronounced resting tremor. They also fail to habituate to confinement and have reduced exploration abilities in open field.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Complexin-1PRO_0000144871Add
BLAST

Proteomic databases

MaxQBiP63040.
PaxDbiP63040.
PRIDEiP63040.

PTM databases

PhosphoSiteiP63040.

Expressioni

Tissue specificityi

Nervous system, and pancreatic islet cells. Present in many brain regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level).3 Publications

Developmental stagei

In the brain, expression starts at P6 and increases to reach a plateau at P20.1 Publication

Gene expression databases

BgeeiP63040.
CleanExiMM_CPLX1.
ExpressionAtlasiP63040. baseline and differential.
GenevestigatoriP63040.

Interactioni

Subunit structurei

Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.By similarity

Protein-protein interaction databases

IntActiP63040. 1 interaction.
MINTiMINT-4091813.

Structurei

3D structure databases

ProteinModelPortaliP63040.
SMRiP63040. Positions 32-72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 7023Interaction with the SNARE complexBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili29 – 6436Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the complexin/synaphin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG85303.
GeneTreeiENSGT00490000043416.
HOGENOMiHOG000017716.
HOVERGENiHBG062792.
InParanoidiP63040.
KOiK15294.
OMAiKYARMEA.
OrthoDBiEOG71G9XG.
PhylomeDBiP63040.
TreeFamiTF315172.

Family and domain databases

InterProiIPR008849. Synaphin.
[Graphical view]
PANTHERiPTHR16705. PTHR16705. 1 hit.
PfamiPF05835. Synaphin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63040-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA
60 70 80 90 100
KYAKMEAERE VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA
110 120 130
IPPGCGDEPE EEDESILDTV IKYLPGPLQD MFKK
Length:134
Mass (Da):15,122
Last modified:August 31, 2004 - v1
Checksum:iBA1B6F074923A3A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841A → V in AAH14803. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38614 mRNA. Translation: BAA07605.1.
AK134820 mRNA. Translation: BAE22299.1.
AK158166 mRNA. Translation: BAE34390.1.
AK159026 mRNA. Translation: BAE34774.1.
BC014803 mRNA. Translation: AAH14803.1.
CCDSiCCDS39206.1.
PIRiS66294.
RefSeqiNP_031782.3. NM_007756.3.
UniGeneiMm.5195.

Genome annotation databases

EnsembliENSMUST00000046892; ENSMUSP00000038502; ENSMUSG00000033615.
GeneIDi12889.
KEGGimmu:12889.
UCSCiuc008yof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38614 mRNA. Translation: BAA07605.1 .
AK134820 mRNA. Translation: BAE22299.1 .
AK158166 mRNA. Translation: BAE34390.1 .
AK159026 mRNA. Translation: BAE34774.1 .
BC014803 mRNA. Translation: AAH14803.1 .
CCDSi CCDS39206.1.
PIRi S66294.
RefSeqi NP_031782.3. NM_007756.3.
UniGenei Mm.5195.

3D structure databases

ProteinModelPortali P63040.
SMRi P63040. Positions 32-72.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P63040. 1 interaction.
MINTi MINT-4091813.

PTM databases

PhosphoSitei P63040.

Proteomic databases

MaxQBi P63040.
PaxDbi P63040.
PRIDEi P63040.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000046892 ; ENSMUSP00000038502 ; ENSMUSG00000033615 .
GeneIDi 12889.
KEGGi mmu:12889.
UCSCi uc008yof.2. mouse.

Organism-specific databases

CTDi 10815.
MGIi MGI:104727. Cplx1.

Phylogenomic databases

eggNOGi NOG85303.
GeneTreei ENSGT00490000043416.
HOGENOMi HOG000017716.
HOVERGENi HBG062792.
InParanoidi P63040.
KOi K15294.
OMAi KYARMEA.
OrthoDBi EOG71G9XG.
PhylomeDBi P63040.
TreeFami TF315172.

Enzyme and pathway databases

Reactomei REACT_205038. Acetylcholine Neurotransmitter Release Cycle.
REACT_229514. Glutamate Neurotransmitter Release Cycle.
REACT_242222. Norepinephrine Neurotransmitter Release Cycle.
REACT_245631. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

ChiTaRSi Cplx1. mouse.
NextBioi 282486.
PROi P63040.
SOURCEi Search...

Gene expression databases

Bgeei P63040.
CleanExi MM_CPLX1.
ExpressionAtlasi P63040. baseline and differential.
Genevestigatori P63040.

Family and domain databases

InterProi IPR008849. Synaphin.
[Graphical view ]
PANTHERi PTHR16705. PTHR16705. 1 hit.
Pfami PF05835. Synaphin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses."
    Takahashi S., Yamamoto H., Matsuda Z., Ogawa M., Yagyu K., Taniguchi T., Miyata T., Kaba H., Higuchi T., Okutani F., Fujimoto S.
    FEBS Lett. 368:455-460(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Inner ear, Medulla oblongata and Visual cortex.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
  5. "Profound ataxia in complexin I knockout mice masks a complex phenotype that includes exploratory and habituation deficits."
    Glynn D., Drew C.J., Reim K., Brose N., Morton A.J.
    Hum. Mol. Genet. 14:2369-2385(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Structurally and functionally unique complexins at retinal ribbon synapses."
    Reim K., Wegmeyer H., Brandstaetter J.H., Xue M., Rosenmund C., Dresbach T., Hofmann K., Brose N.
    J. Cell Biol. 169:669-680(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCPLX1_MOUSE
AccessioniPrimary (citable) accession number: P63040
Secondary accession number(s): O09057
, O09142, Q3UYB3, Q64276, Q91WJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3