Reviewed, UniProtKB/Swiss-Prot P63040 (CPLX1_MOUSE)

Names and origin

Protein names

Recommended name:
    Complexin-1
Alternative name(s):
    Complexin I
      Short name=CPX I
    Synaphin-2
    921-S

Gene names

Name:

Cplx1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Positively regulates a late step in synaptic vesicle exocytosis. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior. Ref.4 Ref.5
Subunit structure	Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A By similarity.
Subcellular location	Cytoplasm › cytosol. Note: Enriched at synaptic-releasing sites in mature neurons. Ref.4 Ref.1
Tissue specificity	Nervous system, and pancreatic islet cells. Present in many brain regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level). Ref.4 Ref.1 Ref.6
Developmental stage	In the brain, expression starts at P6 and increases to reach a plateau at P20. Ref.6
Disruption phenotype	Mice have no obvious brain abnormality, but suffer from severe ataxia with dystonia starting from P7. Adult mice lacking Cplx1 are not capable of coordinated running or swimming and exhibit pronounced resting tremor. They also fail to habituate to confinement and have reduced exploration abilities in open field. Ref.5
Sequence similarities	Belongs to the complexin/synaphin family.

Ontologies

Keywords
Biological process	Exocytosis Neurotransmitter transport Transport
Cellular component	Cytoplasm
Domain	Coiled coil
Gene Ontology (GO)
Biological process	insulin secretion Ref.4 Inferred from mutant phenotype. Source: UniProtKB synaptic vesicle exocytosis Ref.4 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	neurotransmitter transporter activity Ref.4 Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Complexin-1		PRO_0000144871
Regions
	Region	48 – 70	23	Interaction with the SNARE complex By similarity
	Coiled coil	29 – 64	36	Potential
Experimental info
	Sequence conflict	84	1	A → V in AAH14803. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P63040-1 [UniParc]. Last modified August 31, 2004. Version 1. Checksum: BA1B6F074923A3A4	FASTA	134	15,122
	10 20 30 40 50 60 MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE 70 80 90 100 110 120 VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEPE EEDESILDTV 130 IKYLPGPLQD MFKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses." Takahashi S., Yamamoto H., Matsuda Z., Ogawa M., Yagyu K., Taniguchi T., Miyata T., Kaba H., Higuchi T., Okutani F., Fujimoto S. FEBS Lett. 368:455-460(1995) [PubMed: 7635198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: BALB/c. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Inner ear, Medulla oblongata and Visual cortex.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[4]	"Complexin I regulates glucose-induced secretion in pancreatic beta-cells." Abderrahmani A., Niederhauser G., Plaisance V., Roehrich M.-E., Lenain V., Coppola T., Regazzi R., Waeber G. J. Cell Sci. 117:2239-2247(2004) [PubMed: 15126625] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
[5]	"Profound ataxia in complexin I knockout mice masks a complex phenotype that includes exploratory and habituation deficits." Glynn D., Drew C.J., Reim K., Brose N., Morton A.J. Hum. Mol. Genet. 14:2369-2385(2005) [PubMed: 16000319] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"Structurally and functionally unique complexins at retinal ribbon synapses." Reim K., Wegmeyer H., Brandstaetter J.H., Xue M., Rosenmund C., Dresbach T., Hofmann K., Brose N. J. Cell Biol. 169:669-680(2005) [PubMed: 15911881] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38614 mRNA. Translation: BAA07605.1. AK134820 mRNA. Translation: BAE22299.1. AK158166 mRNA. Translation: BAE34390.1. AK159026 mRNA. Translation: BAE34774.1. BC014803 mRNA. Translation: AAH14803.1.
IPI	IPI00132278.
PIR	S66294.
RefSeq	NP_031782.3.
UniGene	Mm.5195
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P63040.
PTM databases
PhosphoSite	P63040.
Proteomic databases
PRIDE	P63040.
Genome annotation databases
Ensembl	ENSMUST00000046892; ENSMUSP00000038502; ENSMUSG00000033615; Mus musculus. [Genome view]
GeneID	12889.
KEGG	mmu:12889.
UCSC	uc008yof.1. mouse.
Organism-specific databases
CTD	12889.
MGI	MGI:104727. Cplx1.
Phylogenomic databases
HOGENOM	P63040.
HOVERGEN	P63040.
OMA	EANAEGS
Gene expression databases
ArrayExpress	P63040.
Bgee	P63040.
CleanEx	MM_CPLX1.
Genevestigator	P63040.
GermOnline	ENSMUSG00000033615. Mus musculus.
Family and domain databases
InterPro	IPR008849. Synaphin. [Graphical view]
PANTHER	PTHR16705. Synaphin. 1 hit.
Pfam	PF05835. Synaphin. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	282486.
SOURCE	Search...

Entry information

Entry name

CPLX1_MOUSE

Accession

Primary (citable) accession number: P63040
Secondary accession number(s): O09057 , O09142, Q3UYB3, Q64276, Q91WJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	August 31, 2004
Last modified:	November 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)