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P63040

- CPLX1_MOUSE

UniProt

P63040 - CPLX1_MOUSE

Protein

Complexin-1

Gene

Cplx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Positively regulates a late step in synaptic vesicle exocytosis. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.2 Publications

    GO - Molecular functioni

    1. neurotransmitter transporter activity Source: UniProtKB
    2. syntaxin-1 binding Source: MGI

    GO - Biological processi

    1. insulin secretion Source: UniProtKB
    2. synaptic vesicle exocytosis Source: UniProtKB

    Keywords - Biological processi

    Exocytosis, Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complexin-1
    Alternative name(s):
    921-S
    Complexin I
    Short name:
    CPX I
    Synaphin-2
    Gene namesi
    Name:Cplx1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:104727. Cplx1.

    Subcellular locationi

    Cytoplasmcytosol 2 Publications
    Note: Enriched at synaptic-releasing sites in mature neurons.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. dendrite Source: MGI
    3. neuronal cell body Source: MGI
    4. SNARE complex Source: UniProtKB
    5. synapse Source: MGI
    6. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mice have no obvious brain abnormality, but suffer from severe ataxia with dystonia starting from P7. Adult mice lacking Cplx1 are not capable of coordinated running or swimming and exhibit pronounced resting tremor. They also fail to habituate to confinement and have reduced exploration abilities in open field.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 134134Complexin-1PRO_0000144871Add
    BLAST

    Proteomic databases

    MaxQBiP63040.
    PaxDbiP63040.
    PRIDEiP63040.

    PTM databases

    PhosphoSiteiP63040.

    Expressioni

    Tissue specificityi

    Nervous system, and pancreatic islet cells. Present in many brain regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level).3 Publications

    Developmental stagei

    In the brain, expression starts at P6 and increases to reach a plateau at P20.1 Publication

    Gene expression databases

    ArrayExpressiP63040.
    BgeeiP63040.
    CleanExiMM_CPLX1.
    GenevestigatoriP63040.

    Interactioni

    Subunit structurei

    Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.By similarity

    Protein-protein interaction databases

    IntActiP63040. 1 interaction.
    MINTiMINT-4091813.

    Structurei

    3D structure databases

    ProteinModelPortaliP63040.
    SMRiP63040. Positions 32-72.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 7023Interaction with the SNARE complexBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili29 – 6436Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complexin/synaphin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG85303.
    GeneTreeiENSGT00490000043416.
    HOGENOMiHOG000017716.
    HOVERGENiHBG062792.
    InParanoidiP63040.
    KOiK15294.
    OMAiKYARMEA.
    OrthoDBiEOG71G9XG.
    PhylomeDBiP63040.
    TreeFamiTF315172.

    Family and domain databases

    InterProiIPR008849. Synaphin.
    [Graphical view]
    PANTHERiPTHR16705. PTHR16705. 1 hit.
    PfamiPF05835. Synaphin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63040-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA    50
    KYAKMEAERE VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA 100
    IPPGCGDEPE EEDESILDTV IKYLPGPLQD MFKK 134
    Length:134
    Mass (Da):15,122
    Last modified:August 31, 2004 - v1
    Checksum:iBA1B6F074923A3A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841A → V in AAH14803. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38614 mRNA. Translation: BAA07605.1.
    AK134820 mRNA. Translation: BAE22299.1.
    AK158166 mRNA. Translation: BAE34390.1.
    AK159026 mRNA. Translation: BAE34774.1.
    BC014803 mRNA. Translation: AAH14803.1.
    CCDSiCCDS39206.1.
    PIRiS66294.
    RefSeqiNP_031782.3. NM_007756.3.
    UniGeneiMm.5195.

    Genome annotation databases

    EnsembliENSMUST00000046892; ENSMUSP00000038502; ENSMUSG00000033615.
    GeneIDi12889.
    KEGGimmu:12889.
    UCSCiuc008yof.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38614 mRNA. Translation: BAA07605.1 .
    AK134820 mRNA. Translation: BAE22299.1 .
    AK158166 mRNA. Translation: BAE34390.1 .
    AK159026 mRNA. Translation: BAE34774.1 .
    BC014803 mRNA. Translation: AAH14803.1 .
    CCDSi CCDS39206.1.
    PIRi S66294.
    RefSeqi NP_031782.3. NM_007756.3.
    UniGenei Mm.5195.

    3D structure databases

    ProteinModelPortali P63040.
    SMRi P63040. Positions 32-72.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P63040. 1 interaction.
    MINTi MINT-4091813.

    PTM databases

    PhosphoSitei P63040.

    Proteomic databases

    MaxQBi P63040.
    PaxDbi P63040.
    PRIDEi P63040.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046892 ; ENSMUSP00000038502 ; ENSMUSG00000033615 .
    GeneIDi 12889.
    KEGGi mmu:12889.
    UCSCi uc008yof.2. mouse.

    Organism-specific databases

    CTDi 10815.
    MGIi MGI:104727. Cplx1.

    Phylogenomic databases

    eggNOGi NOG85303.
    GeneTreei ENSGT00490000043416.
    HOGENOMi HOG000017716.
    HOVERGENi HBG062792.
    InParanoidi P63040.
    KOi K15294.
    OMAi KYARMEA.
    OrthoDBi EOG71G9XG.
    PhylomeDBi P63040.
    TreeFami TF315172.

    Enzyme and pathway databases

    Reactomei REACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Miscellaneous databases

    NextBioi 282486.
    PROi P63040.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63040.
    Bgeei P63040.
    CleanExi MM_CPLX1.
    Genevestigatori P63040.

    Family and domain databases

    InterProi IPR008849. Synaphin.
    [Graphical view ]
    PANTHERi PTHR16705. PTHR16705. 1 hit.
    Pfami PF05835. Synaphin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses."
      Takahashi S., Yamamoto H., Matsuda Z., Ogawa M., Yagyu K., Taniguchi T., Miyata T., Kaba H., Higuchi T., Okutani F., Fujimoto S.
      FEBS Lett. 368:455-460(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: BALB/c.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Inner ear, Medulla oblongata and Visual cortex.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
    5. "Profound ataxia in complexin I knockout mice masks a complex phenotype that includes exploratory and habituation deficits."
      Glynn D., Drew C.J., Reim K., Brose N., Morton A.J.
      Hum. Mol. Genet. 14:2369-2385(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Structurally and functionally unique complexins at retinal ribbon synapses."
      Reim K., Wegmeyer H., Brandstaetter J.H., Xue M., Rosenmund C., Dresbach T., Hofmann K., Brose N.
      J. Cell Biol. 169:669-680(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiCPLX1_MOUSE
    AccessioniPrimary (citable) accession number: P63040
    Secondary accession number(s): O09057
    , O09142, Q3UYB3, Q64276, Q91WJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3