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Protein

60 kDa heat shock protein, mitochondrial

Gene

Hspd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75ATPBy similarity1
Binding sitei440ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei520ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi111 – 115ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone, Hydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1268020 Mitochondrial protein import

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa heat shock protein, mitochondrial (EC:3.6.4.9)
Alternative name(s):
60 kDa chaperonin
Chaperonin 60
Short name:
CPN60
HSP-65
Heat shock protein 60
Short name:
HSP-60
Short name:
Hsp60
Mitochondrial matrix protein P1
Gene namesi
Name:Hspd1
Synonyms:Hsp60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:96242 Hspd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26Mitochondrion1 PublicationAdd BLAST26
ChainiPRO_000000502727 – 57360 kDa heat shock protein, mitochondrialAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31N6-succinyllysineCombined sources1
Modified residuei67PhosphoserineBy similarity1
Modified residuei70PhosphoserineCombined sources1
Modified residuei75N6-acetyllysineCombined sources1
Modified residuei82N6-acetyllysine; alternateBy similarity1
Modified residuei82N6-succinyllysine; alternateCombined sources1
Modified residuei87N6-acetyllysineCombined sources1
Modified residuei90PhosphotyrosineBy similarity1
Modified residuei91N6-acetyllysineCombined sources1
Modified residuei125N6-acetyllysine; alternateCombined sources1
Modified residuei125N6-succinyllysine; alternateCombined sources1
Modified residuei130N6-acetyllysineCombined sources1
Modified residuei133N6-acetyllysine; alternateCombined sources1
Modified residuei133N6-malonyllysine; alternateBy similarity1
Modified residuei133N6-succinyllysine; alternateCombined sources1
Modified residuei156N6-acetyllysineCombined sources1
Modified residuei191N6-acetyllysine; alternateCombined sources1
Modified residuei191N6-succinyllysine; alternateCombined sources1
Modified residuei202N6-acetyllysine; alternateCombined sources1
Modified residuei202N6-succinyllysine; alternateCombined sources1
Modified residuei205N6-acetyllysine; alternateCombined sources1
Modified residuei205N6-succinyllysine; alternateCombined sources1
Modified residuei218N6-acetyllysine; alternateCombined sources1
Modified residuei218N6-succinyllysine; alternateCombined sources1
Modified residuei236N6-acetyllysine; alternateCombined sources1
Modified residuei236N6-succinyllysine; alternateCombined sources1
Modified residuei249N6-acetyllysineCombined sources1
Modified residuei250N6-acetyllysine; alternateCombined sources1
Modified residuei250N6-succinyllysine; alternateCombined sources1
Modified residuei269N6-acetyllysineCombined sources1
Modified residuei292N6-acetyllysineCombined sources1
Modified residuei301N6-succinyllysineCombined sources1
Modified residuei314N6-acetyllysineCombined sources1
Modified residuei352N6-acetyllysine; alternateCombined sources1
Modified residuei352N6-succinyllysine; alternateCombined sources1
Modified residuei359N6-acetyllysineCombined sources1
Modified residuei389N6-acetyllysineCombined sources1
Modified residuei396N6-acetyllysine; alternateBy similarity1
Modified residuei396N6-succinyllysine; alternateCombined sources1
Modified residuei410PhosphoserineCombined sources1
Modified residuei455N6-acetyllysine; alternateCombined sources1
Modified residuei455N6-succinyllysine; alternateCombined sources1
Modified residuei469N6-acetyllysineCombined sources1
Modified residuei481N6-acetyllysine; alternateCombined sources1
Modified residuei481N6-succinyllysine; alternateCombined sources1
Modified residuei488PhosphoserineBy similarity1
Cross-linki551Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63038
MaxQBiP63038
PaxDbiP63038
PeptideAtlasiP63038
PRIDEiP63038
TopDownProteomicsiP63038-1 [P63038-1]

2D gel databases

REPRODUCTION-2DPAGEiIPI00308885
P63038
SWISS-2DPAGEiP63038
UCD-2DPAGEiP63038

PTM databases

CarbonylDBiP63038
iPTMnetiP63038
PhosphoSitePlusiP63038
SwissPalmiP63038

Miscellaneous databases

PMAP-CutDBiP63038

Expressioni

Gene expression databases

BgeeiENSMUSG00000025980
CleanExiMM_HSPD1
ExpressionAtlasiP63038 baseline and differential
GenevisibleiP63038 MM

Interactioni

Subunit structurei

Homoheptamer arranged in a ring structure. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex (By similarity). Interacts with HRAS (PubMed:1347942). Interacts with ATAD3A. Interacts with ETFBKMT and METTL21B (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200451, 16 interactors
DIPiDIP-31396N
IntActiP63038, 23 interactors
MINTiP63038
STRINGi10090.ENSMUSP00000027123

Structurei

3D structure databases

ProteinModelPortaliP63038
SMRiP63038
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356 Eukaryota
COG0459 LUCA
GeneTreeiENSGT00390000005727
HOVERGENiHBG001982
InParanoidiP63038
KOiK04077
OMAiTDTDKME
OrthoDBiEOG091G04JM
PhylomeDBiP63038
TreeFamiTF300475

Family and domain databases

CDDicd03344 GroEL, 1 hit
Gene3Di1.10.560.10, 2 hits
3.30.260.10, 2 hits
3.50.7.10, 1 hit
HAMAPiMF_00600 CH60, 1 hit
InterProiView protein in InterPro
IPR018370 Chaperonin_Cpn60_CS
IPR001844 Chaprnin_Cpn60
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00298 CHAPERONIN60
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 1 hit
TIGRFAMsiTIGR02348 GroEL, 1 hit
PROSITEiView protein in PROSITE
PS00296 CHAPERONINS_CPN60, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P63038-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRLPTVLRQ MRPVSRALAP HLTRAYAKDV KFGADARALM LQGVDLLADA
60 70 80 90 100
VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD
110 120 130 140 150
VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA
160 170 180 190 200
VIAELKKQSK PVTTPEEIAQ VATISANGDK DIGNIISDAM KKVGRKGVIT
210 220 230 240 250
VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK
260 270 280 290 300
ISSVQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV
310 320 330 340 350
KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL NLNLEDVQAH DLGKVGEVIV
360 370 380 390 400
TKDDAMLLKG KGDKAHIEKR IQEITEQLDI TTSEYEKEKL NERLAKLSDG
410 420 430 440 450
VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA
460 470 480 490 500
LDSLKPANED QKIGIEIIKR ALKIPAMTIA KNAGVEGSLI VEKILQSSSE
510 520 530 540 550
VGYDAMLGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEAVVTEIP
560 570
KEEKDPGMGA MGGMGGGMGG GMF
Length:573
Mass (Da):60,955
Last modified:August 31, 2004 - v1
Checksum:i0014B58B77D0127B
GO
Isoform 2 (identifier: P63038-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-315: Missing.

Note: No experimental confirmation available.
Show »
Length:258
Mass (Da):27,015
Checksum:iDD79DC2838C3771B
GO

Sequence cautioni

The sequence AAI06113 differs from that shown. . Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4L → H AA sequence (PubMed:1347942).Curated1
Sequence conflicti139E → K AA sequence (PubMed:1347942).Curated1
Sequence conflicti251I → F in CAA37653 (PubMed:1979012).Curated1
Sequence conflicti364K → E in BAC40607 (PubMed:16141072).Curated1
Sequence conflicti518I → V in CAA38762 (PubMed:2263486).Curated1
Sequence conflicti527T → Q AA sequence (PubMed:1347942).Curated1
Sequence conflicti547T → V AA sequence (PubMed:1347942).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0250201 – 315Missing in isoform 2. 1 PublicationAdd BLAST315

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55023 mRNA Translation: CAA38762.1
AK088844 mRNA Translation: BAC40607.1
AK143882 mRNA Translation: BAE25581.1
AK146831 mRNA Translation: BAE27466.1
BC016400 mRNA Translation: AAH16400.1
BC018545 mRNA Translation: AAH18545.1
BC106112 mRNA Translation: AAI06113.1 Sequence problems.
X53584 mRNA Translation: CAA37653.1
CCDSiCCDS35569.1 [P63038-1]
PIRiS13084 HHMS60
RefSeqiNP_034607.3, NM_010477.4 [P63038-1]
XP_006495769.1, XM_006495706.2
UniGeneiMm.1777
Mm.439809

Genome annotation databases

EnsembliENSMUST00000027123; ENSMUSP00000027123; ENSMUSG00000025980 [P63038-1]
GeneIDi15510
KEGGimmu:15510
UCSCiuc007bab.2 mouse [P63038-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCH60_MOUSE
AccessioniPrimary (citable) accession number: P63038
Secondary accession number(s): P19226
, P19227, P97602, Q3KQP2, Q3UIP0, Q8C2C7, Q8VEF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: May 23, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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