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Protein

DnaJ homolog subfamily A member 1

Gene

Dnaja1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (By similarity). Co-chaperone for HSPA8/Hsc70. Plays a role in protein transport into mitochondria via its role as co-chaperone (PubMed:10816573).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Zinc 1By similarity1
Metal bindingi137Zinc 1By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi153Zinc 2By similarity1
Metal bindingi177Zinc 2By similarity1
Metal bindingi180Zinc 2By similarity1
Metal bindingi193Zinc 1By similarity1
Metal bindingi196Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 205CR-typeAdd BLAST85

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily A member 1
Alternative name(s):
DnaJ-like protein 1
Heat shock protein J2
Short name:
HSJ-2
Gene namesi
Name:Dnaja1
Synonyms:Hsj2, Rdj1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620942. Dnaja1.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity
  • Cytoplasm 1 Publication
  • Microsome 1 Publication
  • Mitochondrion 1 Publication
  • Nucleus 1 Publication
  • Cytoplasmperinuclear region By similarity

  • Note: Primarily cytoplasmic and associated with microsomes. A minor proportion is associated with nuclei and mitochondria.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710101 – 394DnaJ homolog subfamily A member 1Add BLAST394
PropeptideiPRO_0000396755395 – 397Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66N6-acetyllysineBy similarity1
Modified residuei83PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei381PhosphotyrosineBy similarity1
Modified residuei394Cysteine methyl esterBy similarity1
Lipidationi394S-farnesyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP63036.
PRIDEiP63036.

PTM databases

iPTMnetiP63036.
PhosphoSitePlusiP63036.

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000007029.
GenevisibleiP63036. RN.

Interactioni

Subunit structurei

Identified in a complex with HSPA1B and BAX. Interacts with RNF207.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249221. 3 interactors.
IntActiP63036. 1 interactor.
MINTiMINT-1858935.
STRINGi10116.ENSRNOP00000010061.

Structurei

3D structure databases

ProteinModelPortaliP63036.
SMRiP63036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 68JAdd BLAST63
Repeati134 – 141CXXCXGXG motif8
Repeati150 – 157CXXCXGXG motif8
Repeati177 – 184CXXCXGXG motif8
Repeati193 – 200CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 96Gly-richAdd BLAST22

Sequence similaritiesi

Contains 1 CR-type zinc finger.Curated
Contains 1 J domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 205CR-typeAdd BLAST85

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP63036.
KOiK09502.
OMAiIIEFRVI.
OrthoDBiEOG091G0CAC.
PhylomeDBiP63036.
TreeFamiTF105141.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKETTYYDV LGVKPNATQE ELKKAYRKLA LKYHPDKNPN EGEKFKQISQ
60 70 80 90 100
AYEVLADSKK RELYDKGGEQ AIKEGGAGGG FGSPMDIFDM FFGGGGRMQR
110 120 130 140 150
ERRGKNVVHQ LSVTLEDLYN GATRKLALQK NVICDKCEGR GGKKGAVECC
160 170 180 190 200
PNCRGTGMQI RIHQIGPGMV QQIQSVCMEC QGHGERISPK DRCKSCNGRK
210 220 230 240 250
IVREKKILEV HIDKGMKDGQ KITFHGEGDQ EPGLEPGDII IVLDQKDHAV
260 270 280 290 300
FTRRGEDLFM CMDIQLVEAL CGFQKPISTL DNRTIVITSH PGQIVKHGDI
310 320 330 340 350
KCVLNEGMPI YRRPYEKGRL IIEFKVNFPE NGFLSPDKLS LLEKLLPERK
360 370 380 390
EVEETDEMDQ VELVDFDPNQ ERRRHYNGEA YEDDEHHPRG GVQCQTS
Length:397
Mass (Da):44,868
Last modified:August 31, 2004 - v1
Checksum:i1783CE3D5C4CD558
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53922 mRNA. Translation: AAA98855.1.
BC062009 mRNA. Translation: AAH62009.1.
RefSeqiNP_075223.1. NM_022934.1.
XP_006238161.1. XM_006238099.2.
UniGeneiRn.64562.

Genome annotation databases

EnsembliENSRNOT00000010061; ENSRNOP00000010061; ENSRNOG00000007029.
GeneIDi65028.
KEGGirno:65028.
UCSCiRGD:620942. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53922 mRNA. Translation: AAA98855.1.
BC062009 mRNA. Translation: AAH62009.1.
RefSeqiNP_075223.1. NM_022934.1.
XP_006238161.1. XM_006238099.2.
UniGeneiRn.64562.

3D structure databases

ProteinModelPortaliP63036.
SMRiP63036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249221. 3 interactors.
IntActiP63036. 1 interactor.
MINTiMINT-1858935.
STRINGi10116.ENSRNOP00000010061.

PTM databases

iPTMnetiP63036.
PhosphoSitePlusiP63036.

Proteomic databases

PaxDbiP63036.
PRIDEiP63036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010061; ENSRNOP00000010061; ENSRNOG00000007029.
GeneIDi65028.
KEGGirno:65028.
UCSCiRGD:620942. rat.

Organism-specific databases

CTDi3301.
RGDi620942. Dnaja1.

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP63036.
KOiK09502.
OMAiIIEFRVI.
OrthoDBiEOG091G0CAC.
PhylomeDBiP63036.
TreeFamiTF105141.

Miscellaneous databases

PROiP63036.

Gene expression databases

BgeeiENSRNOG00000007029.
GenevisibleiP63036. RN.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJA1_RAT
AccessioniPrimary (citable) accession number: P63036
Secondary accession number(s): P54102
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.