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Protein

Cytohesin-2

Gene

Cyth2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (By similarity). Involved in neurite growth (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei280Phosphatidylinositol 1,4,5-trisphosphateBy similarity1
Binding sitei291Phosphatidylinositol 1,4,5-trisphosphateBy similarity1
Binding sitei301Phosphatidylinositol 1,4,5-trisphosphateBy similarity1
Binding sitei339Phosphatidylinositol 1,4,5-trisphosphateBy similarity1
Binding sitei350Phosphatidylinositol 1,4,5-trisphosphateBy similarity1
Binding sitei351Phosphatidylinositol 1,4,5-trisphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of dendrite development Source: RGD
  • regulation of ARF protein signal transduction Source: InterPro

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-2
Alternative name(s):
ARF nucleotide-binding site opener
Short name:
Protein ARNO
PH, SEC7 and coiled-coil domain-containing protein 2
Short name:
CLM2
SEC7 homolog B
Gene namesi
Name:Cyth2
Synonyms:Pscd2, Sec7b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620398. Cyth2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201991 – 400Cytohesin-2Add BLAST400

Proteomic databases

PaxDbiP63035.
PRIDEiP63035.

PTM databases

PhosphoSitePlusiP63035.

Expressioni

Tissue specificityi

Present in all tissues tested, with highest protein levels in brain and adrenal.1 Publication

Developmental stagei

On embryonic days 15 (E15) and E18, a weak expression is seen in the mantle and ventricular germinal zones throughout the neuraxis. On postnatal days 0 (P0) and P7, weakly expressed in the gray matter, but not in the white matter, throughout the brain. In the cerebellum, the expression is seen in the external granule cell layer.1 Publication

Interactioni

Subunit structurei

Heteromer. Composed of GRASP, CYTH2 and at least one GRM1. Interacts with ARRB1. Interacts with ARL4D; the interaction is direct (By similarity). Directly interacts with CCDC120 through the coiled coil domain; this interaction stabilizes CCDC120, possibly by preventing its ubiquitination, and is required for neurite growth in a neuroblastoma cell line. Interacts with FRMD4A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250574. 2 interactors.
CORUMiP63035.
IntActiP63035. 1 interactor.
MINTiMINT-1790339.
STRINGi10116.ENSRNOP00000028588.

Structurei

3D structure databases

ProteinModelPortaliP63035.
SMRiP63035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 201SEC7PROSITE-ProRule annotationAdd BLAST130
Domaini259 – 376PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 276Phosphatidylinositol 1,4,5-trisphosphate bindingBy similarity9
Regioni387 – 395C-terminal autoinhibitory regionBy similarity9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 67Sequence analysisAdd BLAST58

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 1,4,5-trisphosphate. The PH domain is necessary and sufficient for plasma membrane relocalization (By similarity).By similarity
Autoinhibited by its C-terminal basic region.By similarity
The coiled coil domain is involved in interaction with CCDC120.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiP63035.
KOiK18441.
PhylomeDBiP63035.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR011993. PH-like_dom_sf.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
IPR035999. Sec7_sf.
PfamiView protein in Pfam
PF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiView protein in PROSITE
PS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.

Sequencei

Sequence statusi: Complete.

P63035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDGVYEPPD LTPEERMELE NIRRRKQELL VEIQRLREEL SEAMSEVEGL
60 70 80 90 100
EANEGSKTLQ RNRKMAMGRK KFNMDPKKGI QFLVEHELLQ NTPEEIARFL
110 120 130 140 150
YKGEGLNKTA IGDYLGEREE LNLSVLHAFV DLHEFTDLNL VQALRQFLWS
160 170 180 190 200
FRLPGEAQKI DRMMEAFAQR YCLCNPGVFQ STDTCYVLSF AVIMLNTSLH
210 220 230 240 250
NPNVRDKPGL ERFVAMNRGI NEGGDLPEDL LRNLYDSIRN EPFKIPEDDG
260 270 280 290 300
NDLTHTFFNP DREGWLLKLG GGRVKTWKRR WFILTDNCLY YFEYTTDKEP
310 320 330 340 350
RGIIPLENLS IREVDDPRKP NCFELYIPNN KGQLIKACKT EADGRVVEGN
360 370 380 390 400
HMVYRISAPT QEEKDEWIKS IQAAVSVDPF YEMLAARKKR ISVKKKQEQP
Length:400
Mass (Da):46,571
Last modified:August 31, 2004 - v1
Checksum:i88F14D422A331A89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83896 mRNA. Translation: AAB41444.1.
RefSeqiNP_446363.1. NM_053911.2.
UniGeneiRn.3732.

Genome annotation databases

GeneIDi116692.
KEGGirno:116692.
UCSCiRGD:620398. rat.

Similar proteinsi

Entry informationi

Entry nameiCYH2_RAT
AccessioniPrimary (citable) accession number: P63035
Secondary accession number(s): O89099, P97695
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: November 22, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome