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Protein

Cytohesin-2

Gene

Cyth2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (By similarity). Involved in neurite growth (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei280 – 2801Phosphatidylinositol 1,4,5-trisphosphateBy similarity
Binding sitei291 – 2911Phosphatidylinositol 1,4,5-trisphosphateBy similarity
Binding sitei301 – 3011Phosphatidylinositol 1,4,5-trisphosphateBy similarity
Binding sitei339 – 3391Phosphatidylinositol 1,4,5-trisphosphateBy similarity
Binding sitei350 – 3501Phosphatidylinositol 1,4,5-trisphosphateBy similarity
Binding sitei351 – 3511Phosphatidylinositol 1,4,5-trisphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of dendrite development Source: RGD
  • regulation of ARF protein signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-2
Alternative name(s):
ARF nucleotide-binding site opener
Short name:
Protein ARNO
PH, SEC7 and coiled-coil domain-containing protein 2
Short name:
CLM2
SEC7 homolog B
Gene namesi
Name:Cyth2
Synonyms:Pscd2, Sec7b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620398. Cyth2.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Cytoplasm By similarity
  • Cell projection By similarity
  • Cell projectiongrowth cone By similarity

  • Note: Recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane. In differentiating neuroblastoma cells, colocalizes with CCDC120 in both neurite shaft and growth cone areas.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • growth cone Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • ruffle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Cytohesin-2PRO_0000120199Add
BLAST

Proteomic databases

PaxDbiP63035.
PRIDEiP63035.

PTM databases

PhosphoSiteiP63035.

Expressioni

Tissue specificityi

Present in all tissues tested, with highest protein levels in brain and adrenal.1 Publication

Developmental stagei

On embryonic days 15 (E15) and E18, a weak expression is seen in the mantle and ventricular germinal zones throughout the neuraxis. On postnatal days 0 (P0) and P7, weakly expressed in the gray matter, but not in the white matter, throughout the brain. In the cerebellum, the expression is seen in the external granule cell layer.1 Publication

Interactioni

Subunit structurei

Heteromer. Composed of GRASP, CYTH2 and at least one GRM1. Interacts with ARRB1. Interacts with ARL4D; the interaction is direct (By similarity). Directly interacts with CCDC120 through the coiled coil domain; this interaction stabilizes CCDC120, possibly by preventing its ubiquitination, and is required for neurite growth in neuroblastoma cells.By similarity

Protein-protein interaction databases

BioGridi250574. 2 interactions.
IntActiP63035. 1 interaction.
MINTiMINT-1790339.
STRINGi10116.ENSRNOP00000028588.

Structurei

3D structure databases

ProteinModelPortaliP63035.
SMRiP63035. Positions 52-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 201130SEC7PROSITE-ProRule annotationAdd
BLAST
Domaini259 – 376118PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 2769Phosphatidylinositol 1,4,5-trisphosphate bindingBy similarity
Regioni387 – 3959C-terminal autoinhibitory regionBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili10 – 6758Sequence analysisAdd
BLAST

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 1,4,5-trisphosphate. The PH domain is necessary and sufficient for plasma membrane relocalization (By similarity).By similarity
Autoinhibited by its C-terminal basic region.By similarity
The coiled coil domain is involved in interaction with CCDC120.By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiP63035.
KOiK18441.
PhylomeDBiP63035.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDGVYEPPD LTPEERMELE NIRRRKQELL VEIQRLREEL SEAMSEVEGL
60 70 80 90 100
EANEGSKTLQ RNRKMAMGRK KFNMDPKKGI QFLVEHELLQ NTPEEIARFL
110 120 130 140 150
YKGEGLNKTA IGDYLGEREE LNLSVLHAFV DLHEFTDLNL VQALRQFLWS
160 170 180 190 200
FRLPGEAQKI DRMMEAFAQR YCLCNPGVFQ STDTCYVLSF AVIMLNTSLH
210 220 230 240 250
NPNVRDKPGL ERFVAMNRGI NEGGDLPEDL LRNLYDSIRN EPFKIPEDDG
260 270 280 290 300
NDLTHTFFNP DREGWLLKLG GGRVKTWKRR WFILTDNCLY YFEYTTDKEP
310 320 330 340 350
RGIIPLENLS IREVDDPRKP NCFELYIPNN KGQLIKACKT EADGRVVEGN
360 370 380 390 400
HMVYRISAPT QEEKDEWIKS IQAAVSVDPF YEMLAARKKR ISVKKKQEQP
Length:400
Mass (Da):46,571
Last modified:August 31, 2004 - v1
Checksum:i88F14D422A331A89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83896 mRNA. Translation: AAB41444.1.
RefSeqiNP_446363.1. NM_053911.2.
UniGeneiRn.3732.

Genome annotation databases

GeneIDi116692.
KEGGirno:116692.
UCSCiRGD:620398. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83896 mRNA. Translation: AAB41444.1.
RefSeqiNP_446363.1. NM_053911.2.
UniGeneiRn.3732.

3D structure databases

ProteinModelPortaliP63035.
SMRiP63035. Positions 52-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250574. 2 interactions.
IntActiP63035. 1 interaction.
MINTiMINT-1790339.
STRINGi10116.ENSRNOP00000028588.

PTM databases

PhosphoSiteiP63035.

Proteomic databases

PaxDbiP63035.
PRIDEiP63035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116692.
KEGGirno:116692.
UCSCiRGD:620398. rat.

Organism-specific databases

CTDi9266.
RGDi620398. Cyth2.

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiP63035.
KOiK18441.
PhylomeDBiP63035.

Miscellaneous databases

PROiP63035.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYH2_RAT
AccessioniPrimary (citable) accession number: P63035
Secondary accession number(s): O89099, P97695
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: July 6, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.