ID   VAMP2_HUMAN             Reviewed;         116 AA.
AC   P63027; P19065;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   24-NOV-2009, entry version 58.
DE   RecName: Full=Vesicle-associated membrane protein 2;
DE            Short=VAMP-2;
DE   AltName: Full=Synaptobrevin-2;
GN   Name=VAMP2; Synonyms=SYB2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91009161; PubMed=1976629;
RA   Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C.;
RT   "Structures and chromosomal localizations of two human genes encoding
RT   synaptobrevins 1 and 2.";
RL   J. Biol. Chem. 265:17267-17273(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Peripheral blood;
RA   Nabokina S.M., Lazo P.A., Mollinedo F.;
RT   "Expression of VAMP genes in human neutrophils.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Blood;
RA   Taruscio D., Zoraqi K.G., Falbo V.;
RT   "Genomic structure of human SYB2 gene.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   TISSUE SPECIFICITY.
RX   MEDLINE=96332494; PubMed=8760387;
RA   Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H.,
RA   Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J.,
RA   Ward C.W.;
RT   "Insulin-responsive tissues contain the core complex protein SNAP-25
RT   (synaptosomal-associated protein 25) A and B isoforms in addition to
RT   syntaxin 4 and synaptobrevins 1 and 2.";
RL   Biochem. J. 317:945-954(1996).
RN   [5]
RP   TOPOLOGY.
RX   MEDLINE=95137000; PubMed=7835332;
RA   Kutay U., Ahnert-Hilger G., Hartmann E., Wiedenmann B., Rapoport T.A.;
RT   "Transport route for synaptobrevin via a novel pathway of insertion
RT   into the endoplasmic reticulum membrane.";
RL   EMBO J. 14:217-223(1995).
RN   [6]
RP   IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RA   Colinge J., Superti-Furga G., Bennett K.L.;
RL   Submitted (OCT-2008) to UniProtKB.
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 34-89 IN COMPLEX WITH BOTB.
RX   MEDLINE=20392548; PubMed=10932255; DOI=10.1038/77997;
RA   Hanson M.A., Stevens R.C.;
RT   "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin
RT   type B at 2.0 A resolution.";
RL   Nat. Struct. Biol. 7:687-692(2000).
CC   -!- FUNCTION: Involved in the targeting and/or fusion of transport
CC       vesicles to their target membrane.
CC   -!- SUBUNIT: Interacts with VAPA and VAPB.
CC   -!- INTERACTION:
CC       O94806:PRKD3; NbExp=2; IntAct=EBI-520113, EBI-1255366;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle,
CC       synaptic vesicle membrane; Single-pass type IV membrane protein.
CC       Cell junction, synapse, synaptosome. Note=Neuronal synaptic
CC       vesicles.
CC   -!- TISSUE SPECIFICITY: Nervous system and skeletal muscle.
CC   -!- SIMILARITY: Belongs to the synaptobrevin family.
CC   -!- SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.
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DR   EMBL; M36205; AAA60604.1; -; Genomic_DNA.
DR   EMBL; M36201; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; M36202; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; M36203; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; M36204; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; AJ225044; CAA12385.1; -; mRNA.
DR   EMBL; AF135372; AAF15551.1; -; Genomic_DNA.
DR   IPI; IPI00553138; -.
DR   PIR; B38315; B38315.
DR   UniGene; Hs.25348; -.
DR   PDB; 3FIE; X-ray; 2.10 A; C/D=22-57.
DR   PDB; 3FII; X-ray; 2.17 A; B=27-57.
DR   PDBsum; 3FIE; -.
DR   PDBsum; 3FII; -.
DR   SMR; P63027; 25-93.
DR   DisProt; DP00069; -.
DR   IntAct; P63027; 2.
DR   STRING; P63027; -.
DR   TCDB; 1.F.1.1.1; synaptosomal vesicle fusion pore (SVF-Pore) family.
DR   PhosphoSite; P63027; -.
DR   PRIDE; P63027; -.
DR   Ensembl; ENST00000316509; ENSP00000314214; ENSG00000220205; Homo sapiens.
DR   GeneCards; GC17M007987; -.
DR   H-InvDB; HIX0020382; -.
DR   HGNC; HGNC:12643; VAMP2.
DR   MIM; 185881; gene.
DR   PharmGKB; PA37267; -.
DR   HOGENOM; P63027; -.
DR   HOVERGEN; P63027; -.
DR   Pathway_Interaction_DB; botulinumtoxinpathway; Effects of Botulinum toxin.
DR   Pathway_Interaction_DB; insulin_glucose_pathway; Insulin-mediated glucose transport.
DR   Reactome; REACT_11123; Membrane Trafficking.
DR   Reactome; REACT_11184; Botulinum neurotoxicity.
DR   Reactome; REACT_13685; Synaptic Transmission.
DR   Reactome; REACT_1505; Integration of energy metabolism.
DR   Reactome; REACT_15380; Diabetes pathways.
DR   DrugBank; DB00042; Botulinum Toxin Type B.
DR   ArrayExpress; P63027; -.
DR   Bgee; P63027; -.
DR   CleanEx; HS_VAMP2; -.
DR   Genevestigator; P63027; -.
DR   GermOnline; ENSG00000179036; Homo sapiens.
DR   GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR   GO; GO:0060203; C:clathrin sculpted glutamate transport vesic...; EXP:Reactome.
DR   GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR   GO; GO:0030667; C:secretory granule membrane; EXP:Reactome.
DR   GO; GO:0045202; C:synapse; IEA:UniProtKB-KW.
DR   GO; GO:0019717; C:synaptosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0016044; P:membrane organization; EXP:Reactome.
DR   GO; GO:0006892; P:post-Golgi vesicle-mediated transport; EXP:Reactome.
DR   InterPro; IPR001388; Synaptobrevin.
DR   InterPro; IPR016444; Synaptobrevin_met/fun.
DR   Pfam; PF00957; Synaptobrevin; 1.
DR   PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR   PRINTS; PR00219; SYNAPTOBREVN.
DR   PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR   PROSITE; PS50892; V_SNARE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Cell junction; Coiled coil;
KW   Complete proteome; Cytoplasmic vesicle; Membrane; Phosphoprotein;
KW   Synapse; Synaptosome; Transmembrane.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    116       Vesicle-associated membrane protein 2.
FT                                /FTId=PRO_0000206723.
FT   TOPO_DOM      2     94       Cytoplasmic (Potential).
FT   TRANSMEM     95    114       Anchor for type IV membrane protein
FT                                (Potential).
FT   TOPO_DOM    115    116       Vesicular (Potential).
FT   DOMAIN       31     91       v-SNARE coiled-coil homology.
FT   MOD_RES       2      2       N-acetylserine (By similarity).
FT   MOD_RES      75     75       Phosphoserine (By similarity).
SQ   SEQUENCE   116 AA;  12649 MW;  98C679C4F6F1B5A8 CRC64;
     MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL
     SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFSS
//