ID   VAMP2_HUMAN             Reviewed;         116 AA.
AC   P63027; P19065; Q9BUC2;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   09-FEB-2010, sequence version 3.
DT   25-JAN-2012, entry version 79.
DE   RecName: Full=Vesicle-associated membrane protein 2;
DE            Short=VAMP-2;
DE   AltName: Full=Synaptobrevin-2;
GN   Name=VAMP2; Synonyms=SYB2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91009161; PubMed=1976629;
RA   Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C.;
RT   "Structures and chromosomal localizations of two human genes encoding
RT   synaptobrevins 1 and 2.";
RL   J. Biol. Chem. 265:17267-17273(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Peripheral blood;
RA   Nabokina S.M., Lazo P.A., Mollinedo F.;
RT   "Expression of VAMP genes in human neutrophils.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Blood;
RA   Taruscio D., Zoraqi K.G., Falbo V.;
RT   "Genomic structure of human SYB2 gene.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Cerebellum;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neuroblastoma, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   MEDLINE=96332494; PubMed=8760387;
RA   Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H.,
RA   Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J.,
RA   Ward C.W.;
RT   "Insulin-responsive tissues contain the core complex protein SNAP-25
RT   (synaptosomal-associated protein 25) A and B isoforms in addition to
RT   syntaxin 4 and synaptobrevins 1 and 2.";
RL   Biochem. J. 317:945-954(1996).
RN   [8]
RP   TOPOLOGY.
RX   MEDLINE=95137000; PubMed=7835332;
RA   Kutay U., Ahnert-Hilger G., Hartmann E., Wiedenmann B., Rapoport T.A.;
RT   "Transport route for synaptobrevin via a novel pathway of insertion
RT   into the endoplasmic reticulum membrane.";
RL   EMBO J. 14:217-223(1995).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [10]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 34-89 IN COMPLEX WITH BOTB.
RX   MEDLINE=20392548; PubMed=10932255; DOI=10.1038/77997;
RA   Hanson M.A., Stevens R.C.;
RT   "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin
RT   type B at 2.0 A resolution.";
RL   Nat. Struct. Biol. 7:687-692(2000).
CC   -!- FUNCTION: Involved in the targeting and/or fusion of transport
CC       vesicles to their target membrane.
CC   -!- SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2
CC       and STX1A. This complex binds to CPLX1. Interacts with BVES and
CC       STX4 (By similarity). Interacts with VAPA and VAPB.
CC   -!- INTERACTION:
CC       O94806:PRKD3; NbExp=7; IntAct=EBI-520113, EBI-1255366;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle,
CC       synaptic vesicle membrane; Single-pass type IV membrane protein.
CC       Cell junction, synapse, synaptosome. Note=Neuronal synaptic
CC       vesicles.
CC   -!- TISSUE SPECIFICITY: Nervous system and skeletal muscle.
CC   -!- SIMILARITY: Belongs to the synaptobrevin family.
CC   -!- SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.
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DR   EMBL; M36205; AAA60604.1; -; Genomic_DNA.
DR   EMBL; M36201; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; M36202; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; M36203; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; M36204; AAA60604.1; JOINED; Genomic_DNA.
DR   EMBL; AJ225044; CAA12385.1; -; mRNA.
DR   EMBL; AF135372; AAF15551.1; -; Genomic_DNA.
DR   EMBL; AK289555; BAF82244.1; -; mRNA.
DR   EMBL; CH471108; EAW90087.1; -; Genomic_DNA.
DR   EMBL; BC002737; AAH02737.3; -; mRNA.
DR   EMBL; BC019608; AAH19608.1; -; mRNA.
DR   EMBL; BC033870; AAH33870.1; -; mRNA.
DR   IPI; IPI00553138; -.
DR   PIR; B38315; B38315.
DR   RefSeq; NP_055047.2; NM_014232.2.
DR   UniGene; Hs.25348; -.
DR   PDB; 3FIE; X-ray; 2.10 A; C/D=22-57.
DR   PDB; 3FII; X-ray; 2.17 A; B=27-57.
DR   PDB; 3RK2; X-ray; 2.20 A; A/E=28-60.
DR   PDB; 3RK3; X-ray; 3.50 A; A=28-60.
DR   PDB; 3RL0; X-ray; 3.80 A; A/E/I/M/Q/U/Y/c=28-60.
DR   PDBsum; 3FIE; -.
DR   PDBsum; 3FII; -.
DR   PDBsum; 3RK2; -.
DR   PDBsum; 3RK3; -.
DR   PDBsum; 3RL0; -.
DR   ProteinModelPortal; P63027; -.
DR   SMR; P63027; 30-116.
DR   DisProt; DP00069; -.
DR   DIP; DIP-39072N; -.
DR   IntAct; P63027; 2.
DR   STRING; P63027; -.
DR   TCDB; 1.F.1.1.1; synaptosomal vesicle fusion pore (SVF-Pore) family.
DR   PhosphoSite; P63027; -.
DR   DMDM; 288558837; -.
DR   PRIDE; P63027; -.
DR   Ensembl; ENST00000316509; ENSP00000314214; ENSG00000220205.
DR   GeneID; 6844; -.
DR   KEGG; hsa:6844; -.
DR   CTD; 6844; -.
DR   GeneCards; GC17M008054; -.
DR   HGNC; HGNC:12643; VAMP2.
DR   MIM; 185881; gene.
DR   neXtProt; NX_P63027; -.
DR   PharmGKB; PA37267; -.
DR   eggNOG; prNOG21021; -.
DR   GeneTree; ENSGT00550000074449; -.
DR   HOVERGEN; HBG006675; -.
DR   OrthoDB; EOG43JC6B; -.
DR   Pathway_Interaction_DB; botulinumtoxinpathway; Effects of Botulinum toxin.
DR   Pathway_Interaction_DB; insulin_glucose_pathway; Insulin-mediated glucose transport.
DR   Reactome; REACT_111217; Metabolism.
DR   Reactome; REACT_11123; Membrane Trafficking.
DR   Reactome; REACT_13685; Neuronal System.
DR   Reactome; REACT_15380; Diabetes pathways.
DR   DrugBank; DB00042; Botulinum Toxin Type B.
DR   ArrayExpress; P63027; -.
DR   Bgee; P63027; -.
DR   CleanEx; HS_VAMP2; -.
DR   Genevestigator; P63027; -.
DR   GermOnline; ENSG00000179036; Homo sapiens.
DR   GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR   GO; GO:0061202; C:clathrin sculpted gamma-aminobutyric acid transport vesicle membrane; TAS:Reactome.
DR   GO; GO:0060203; C:clathrin sculpted glutamate transport vesicle membrane; TAS:Reactome.
DR   GO; GO:0070083; C:clathrin sculpted monoamine transport vesicle membrane; TAS:Reactome.
DR   GO; GO:0030666; C:endocytic vesicle membrane; TAS:Reactome.
DR   GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR   GO; GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019717; C:synaptosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
DR   GO; GO:0014047; P:glutamate secretion; TAS:Reactome.
DR   GO; GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
DR   GO; GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
DR   InterPro; IPR001388; Synaptobrevin.
DR   InterPro; IPR016444; Synaptobrevin_met/fun.
DR   KO; K13504; -.
DR   Pfam; PF00957; Synaptobrevin; 1.
DR   PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR   PRINTS; PR00219; SYNAPTOBREVN.
DR   PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR   PROSITE; PS50892; V_SNARE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Cell junction; Coiled coil;
KW   Complete proteome; Cytoplasmic vesicle; Membrane; Phosphoprotein;
KW   Reference proteome; Synapse; Synaptosome; Transmembrane;
KW   Transmembrane helix.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    116       Vesicle-associated membrane protein 2.
FT                                /FTId=PRO_0000206723.
FT   TOPO_DOM      2     94       Cytoplasmic (Potential).
FT   TRANSMEM     95    114       Helical; Anchor for type IV membrane
FT                                protein; (Potential).
FT   TOPO_DOM    115    116       Vesicular (Potential).
FT   DOMAIN       31     91       v-SNARE coiled-coil homology.
FT   MOD_RES       2      2       N-acetylserine (By similarity).
FT   MOD_RES      75     75       Phosphoserine (By similarity).
FT   CONFLICT    116    116       T -> S (in Ref. 1; AAA60604, 2; CAA12385
FT                                and 3; AAF15551).
FT   STRAND       34     36
FT   TURN         47     49
FT   HELIX        50     53
SQ   SEQUENCE   116 AA;  12663 MW;  9CD679C4F6F1B5A8 CRC64;
     MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL
     SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFST
//