Reviewed,
UniProtKB/Swiss-Prot P63027 (VAMP2_HUMAN)
Last modified
October 13, 2009.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Vesicle-associated membrane protein 2 Short name=VAMP-2 Alternative name(s): Synaptobrevin-2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 116 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane. |
| Subunit structure | Interacts with VAPA and VAPB. |
| Subcellular location | Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction › synapse › synaptosome. Note: Neuronal synaptic vesicles. |
| Tissue specificity | Nervous system and skeletal muscle. Ref.4 |
| Sequence similarities | Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome |
| Domain | Coiled coil Transmembrane |
| PTM | Acetylation Phosphoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | membrane organization Inferred from Experiment. Source: Reactome post-Golgi vesicle-mediated transportInferred from Experiment. Source: Reactome |
| Cellular component | cell junction Inferred from electronic annotation. Source: UniProtKB-KW clathrin sculpted glutamate transport vesicle membraneInferred from Experiment. Source: Reactome integral to plasma membrane Ref.1Traceable author statement. Source: ProtInc secretory granule membraneInferred from Experiment. Source: Reactome synapseInferred from electronic annotation. Source: UniProtKB-KW synaptosomeInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 116 | 115 | Vesicle-associated membrane protein 2 | PRO_0000206723 | |||||
Regions | |||||||||
| Topological domain | 2 – 94 | 93 | Cytoplasmic Potential | ||||||
| Transmembrane | 95 – 114 | 20 | Anchor for type IV membrane protein Potential | ||||||
| Topological domain | 115 – 116 | 2 | Vesicular Potential | ||||||
| Domain | 31 – 91 | 61 | v-SNARE coiled-coil homology | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylserine By similarity | ||||||
| Modified residue | 75 | 1 | Phosphoserine By similarity | ||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2." Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C. J. Biol. Chem. 265:17267-17273(1990) [PubMed: 1976629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Expression of VAMP genes in human neutrophils." Nabokina S.M., Lazo P.A., Mollinedo F. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood. |
| [3] | "Genomic structure of human SYB2 gene." Taruscio D., Zoraqi K.G., Falbo V. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood. |
| [4] | "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H., Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J., Ward C.W. Biochem. J. 317:945-954(1996) [PubMed: 8760387] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [5] | "Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane." Kutay U., Ahnert-Hilger G., Hartmann E., Wiedenmann B., Rapoport T.A. EMBO J. 14:217-223(1995) [PubMed: 7835332] [Abstract] Cited for: TOPOLOGY. |
| [6] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [7] | "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution." Hanson M.A., Stevens R.C. Nat. Struct. Biol. 7:687-692(2000) [PubMed: 10932255] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 34-89 IN COMPLEX WITH BOTB. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
M36205 M36204 Genomic DNA. Translation: AAA60604.1. AJ225044 mRNA. Translation: CAA12385.1. AF135372 Genomic DNA. Translation: AAF15551.1. | |||||||||||||||||||
| IPI | IPI00553138. | ||||||||||||||||||
| PIR | B38315. | ||||||||||||||||||
| UniGene | Hs.25348 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| |||||||||||||||||||
| SMR | P63027. Positions 25-93. | ||||||||||||||||||
| DisProt | DP00069. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P63027. 2 interactions. | ||||||||||||||||||
| STRING | P63027. | ||||||||||||||||||
Protein family/group databases | |||||||||||||||||||
| TCDB | 1.F.1.1.1. synaptosomal vesicle fusion pore (SVF-Pore) family. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P63027. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PRIDE | P63027. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000316509; ENSP00000314214; ENSG00000220205; Homo sapiens. [Genome view] ENST00000404970; ENSP00000385595; ENSG00000220205; Homo sapiens. [Genome view] | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| GeneCards | GC17M007987. | ||||||||||||||||||
| H-InvDB | HIX0020382. | ||||||||||||||||||
| HGNC | HGNC:12643. VAMP2. | ||||||||||||||||||
| MIM | 185881. gene. | ||||||||||||||||||
| PharmGKB | PA37267. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOGENOM | P63027. | ||||||||||||||||||
| HOVERGEN | P63027. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| Pathway_Interaction_DB | botulinumtoxinpathway. Effects of Botulinum toxin. insulin_glucose_pathway. Insulin-mediated glucose transport. | ||||||||||||||||||
| Reactome | REACT_11123. Membrane Trafficking. REACT_11184. Botulinum neurotoxicity. REACT_13685. Synaptic Transmission. REACT_1505. Integration of energy metabolism. REACT_15380. Diabetes pathways. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| Bgee | P63027. | ||||||||||||||||||
| CleanEx | HS_VAMP2. | ||||||||||||||||||
| Genevestigator | P63027. | ||||||||||||||||||
| GermOnline | ENSG00000179036. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view] | ||||||||||||||||||
| Pfam | PF00957. Synaptobrevin. 1 hit. [Graphical view] | ||||||||||||||||||
| PIRSF | PIRSF005409. Synaptobrevin_euk. 1 hit. | ||||||||||||||||||
| PRINTS | PR00219. SYNAPTOBREVN. | ||||||||||||||||||
| ProDom | PD001229. Synaptobrevin. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||
| PROSITE | PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| DrugBank | DB00042. Botulinum Toxin Type B. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | VAMP2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P63027 Secondary accession number(s): P19065 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 17 Human chromosome 17: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


