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P63027

- VAMP2_HUMAN

UniProt

P63027 - VAMP2_HUMAN

Protein

Vesicle-associated membrane protein 2

Gene

VAMP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. energy reserve metabolic process Source: Reactome
    3. glutamate secretion Source: Reactome
    4. membrane organization Source: Reactome
    5. neurotransmitter secretion Source: Reactome
    6. post-Golgi vesicle-mediated transport Source: Reactome
    7. regulation of insulin secretion Source: Reactome
    8. small molecule metabolic process Source: Reactome
    9. synaptic transmission Source: Reactome

    Enzyme and pathway databases

    ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_147867. Translocation of GLUT4 to the plasma membrane.
    REACT_15293. Dopamine Neurotransmitter Release Cycle.
    REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
    REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
    REACT_15425. Serotonin Neurotransmitter Release Cycle.
    REACT_15550. Insulin processing.
    REACT_18325. Regulation of insulin secretion.
    REACT_19187. Clathrin derived vesicle budding.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.
    REACT_200617. Toxicity of botulinum toxin type D (BoNT/D).
    REACT_200659. Toxicity of botulinum toxin type F (BoNT/F).
    REACT_200743. Toxicity of botulinum toxin type G (BoNT/G).
    REACT_200782. Toxicity of botulinum toxin type B (BoNT/B).
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Protein family/group databases

    TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-associated membrane protein 2
    Short name:
    VAMP-2
    Alternative name(s):
    Synaptobrevin-2
    Gene namesi
    Name:VAMP2
    Synonyms:SYB2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:12643. VAMP2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. clathrin-coated vesicle Source: UniProtKB
    3. clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane Source: Reactome
    4. clathrin-sculpted glutamate transport vesicle membrane Source: Reactome
    5. clathrin-sculpted monoamine transport vesicle membrane Source: Reactome
    6. endocytic vesicle membrane Source: Reactome
    7. extracellular vesicular exosome Source: UniProt
    8. integral component of plasma membrane Source: ProtInc
    9. membrane Source: MGI
    10. neuron projection terminus Source: ParkinsonsUK-UCL
    11. plasma membrane Source: Reactome
    12. secretory granule membrane Source: Reactome
    13. SNARE complex Source: UniProtKB
    14. synaptic vesicle Source: ParkinsonsUK-UCL
    15. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37267.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 116115Vesicle-associated membrane protein 2PRO_0000206723Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP63027.
    PRIDEiP63027.

    PTM databases

    PhosphoSiteiP63027.

    Expressioni

    Tissue specificityi

    Nervous system and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiP63027.
    BgeeiP63027.
    CleanExiHS_VAMP2.
    GenevestigatoriP63027.

    Interactioni

    Subunit structurei

    Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with BVES and STX4 By similarity. Interacts with VAPA and VAPB.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    D2KHQ92EBI-520113,EBI-7604762From a different organism.
    FA7GBG32EBI-520113,EBI-7604673From a different organism.
    PicalmO550122EBI-520113,EBI-915601From a different organism.
    PRKD3O948067EBI-520113,EBI-1255366

    Protein-protein interaction databases

    BioGridi112711. 61 interactions.
    DIPiDIP-39072N.
    IntActiP63027. 7 interactions.
    MINTiMINT-4824900.
    STRINGi9606.ENSP00000314214.

    Structurei

    Secondary structure

    1
    116
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Turni47 – 493
    Helixi50 – 534

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FIEX-ray2.10C/D22-57[»]
    3FIIX-ray2.17B27-57[»]
    3RK2X-ray2.20A/E28-60[»]
    3RK3X-ray3.50A28-60[»]
    3RL0X-ray3.80A/E/I/M/Q/U/Y/c28-60[»]
    DisProtiDP00069.
    ProteinModelPortaliP63027.
    SMRiP63027. Positions 30-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63027.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 9493CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini115 – 1162VesicularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei95 – 11420Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 9161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the synaptobrevin family.Curated
    Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000042711.
    HOVERGENiHBG006675.
    KOiK13504.
    OrthoDBiEOG7MSMRJ.
    PhylomeDBiP63027.
    TreeFamiTF313666.

    Family and domain databases

    InterProiIPR001388. Synaptobrevin.
    IPR016444. Synaptobrevin/VAMP_met/fun.
    [Graphical view]
    PfamiPF00957. Synaptobrevin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
    PRINTSiPR00219. SYNAPTOBREVN.
    PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63027-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV    50
    DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG 100
    VICAIILIII IVYFST 116
    Length:116
    Mass (Da):12,663
    Last modified:February 9, 2010 - v3
    Checksum:i9CD679C4F6F1B5A8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti116 – 1161T → S in AAA60604. (PubMed:1976629)Curated
    Sequence conflicti116 – 1161T → S in CAA12385. 1 PublicationCurated
    Sequence conflicti116 – 1161T → S in AAF15551. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36205
    , M36201, M36202, M36203, M36204 Genomic DNA. Translation: AAA60604.1.
    AJ225044 mRNA. Translation: CAA12385.1.
    AF135372 Genomic DNA. Translation: AAF15551.1.
    AK289555 mRNA. Translation: BAF82244.1.
    CH471108 Genomic DNA. Translation: EAW90087.1.
    BC002737 mRNA. Translation: AAH02737.3.
    BC019608 mRNA. Translation: AAH19608.1.
    BC033870 mRNA. Translation: AAH33870.1.
    CCDSiCCDS32561.1.
    PIRiB38315.
    RefSeqiNP_055047.2. NM_014232.2.
    UniGeneiHs.25348.

    Genome annotation databases

    EnsembliENST00000316509; ENSP00000314214; ENSG00000220205.
    GeneIDi6844.
    KEGGihsa:6844.
    UCSCiuc010cnt.1. human.

    Polymorphism databases

    DMDMi288558837.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36205
    , M36201 , M36202 , M36203 , M36204 Genomic DNA. Translation: AAA60604.1 .
    AJ225044 mRNA. Translation: CAA12385.1 .
    AF135372 Genomic DNA. Translation: AAF15551.1 .
    AK289555 mRNA. Translation: BAF82244.1 .
    CH471108 Genomic DNA. Translation: EAW90087.1 .
    BC002737 mRNA. Translation: AAH02737.3 .
    BC019608 mRNA. Translation: AAH19608.1 .
    BC033870 mRNA. Translation: AAH33870.1 .
    CCDSi CCDS32561.1.
    PIRi B38315.
    RefSeqi NP_055047.2. NM_014232.2.
    UniGenei Hs.25348.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FIE X-ray 2.10 C/D 22-57 [» ]
    3FII X-ray 2.17 B 27-57 [» ]
    3RK2 X-ray 2.20 A/E 28-60 [» ]
    3RK3 X-ray 3.50 A 28-60 [» ]
    3RL0 X-ray 3.80 A/E/I/M/Q/U/Y/c 28-60 [» ]
    DisProti DP00069.
    ProteinModelPortali P63027.
    SMRi P63027. Positions 30-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112711. 61 interactions.
    DIPi DIP-39072N.
    IntActi P63027. 7 interactions.
    MINTi MINT-4824900.
    STRINGi 9606.ENSP00000314214.

    Chemistry

    ChEMBLi CHEMBL2364160.
    DrugBanki DB00042. Botulinum Toxin Type B.

    Protein family/group databases

    TCDBi 1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

    PTM databases

    PhosphoSitei P63027.

    Polymorphism databases

    DMDMi 288558837.

    Proteomic databases

    MaxQBi P63027.
    PRIDEi P63027.

    Protocols and materials databases

    DNASUi 6844.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000316509 ; ENSP00000314214 ; ENSG00000220205 .
    GeneIDi 6844.
    KEGGi hsa:6844.
    UCSCi uc010cnt.1. human.

    Organism-specific databases

    CTDi 6844.
    GeneCardsi GC17M008062.
    HGNCi HGNC:12643. VAMP2.
    MIMi 185881. gene.
    neXtProti NX_P63027.
    PharmGKBi PA37267.
    GenAtlasi Search...

    Phylogenomic databases

    HOGENOMi HOG000042711.
    HOVERGENi HBG006675.
    KOi K13504.
    OrthoDBi EOG7MSMRJ.
    PhylomeDBi P63027.
    TreeFami TF313666.

    Enzyme and pathway databases

    Reactomei REACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_147867. Translocation of GLUT4 to the plasma membrane.
    REACT_15293. Dopamine Neurotransmitter Release Cycle.
    REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
    REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
    REACT_15425. Serotonin Neurotransmitter Release Cycle.
    REACT_15550. Insulin processing.
    REACT_18325. Regulation of insulin secretion.
    REACT_19187. Clathrin derived vesicle budding.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.
    REACT_200617. Toxicity of botulinum toxin type D (BoNT/D).
    REACT_200659. Toxicity of botulinum toxin type F (BoNT/F).
    REACT_200743. Toxicity of botulinum toxin type G (BoNT/G).
    REACT_200782. Toxicity of botulinum toxin type B (BoNT/B).
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Miscellaneous databases

    ChiTaRSi VAMP2. human.
    EvolutionaryTracei P63027.
    GeneWikii VAMP2.
    GenomeRNAii 6844.
    NextBioi 26719.
    PROi P63027.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63027.
    Bgeei P63027.
    CleanExi HS_VAMP2.
    Genevestigatori P63027.

    Family and domain databases

    InterProi IPR001388. Synaptobrevin.
    IPR016444. Synaptobrevin/VAMP_met/fun.
    [Graphical view ]
    Pfami PF00957. Synaptobrevin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005409. Synaptobrevin_euk. 1 hit.
    PRINTSi PR00219. SYNAPTOBREVN.
    PROSITEi PS00417. SYNAPTOBREVIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2."
      Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C.
      J. Biol. Chem. 265:17267-17273(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Expression of VAMP genes in human neutrophils."
      Nabokina S.M., Lazo P.A., Mollinedo F.
      Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Peripheral blood.
    3. "Genomic structure of human SYB2 gene."
      Taruscio D., Zoraqi K.G., Falbo V.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Blood.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Neuroblastoma and Testis.
    7. "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2."
      Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H., Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J., Ward C.W.
      Biochem. J. 317:945-954(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane."
      Kutay U., Ahnert-Hilger G., Hartmann E., Wiedenmann B., Rapoport T.A.
      EMBO J. 14:217-223(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    9. "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution."
      Hanson M.A., Stevens R.C.
      Nat. Struct. Biol. 7:687-692(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 34-89 IN COMPLEX WITH BOTB.

    Entry informationi

    Entry nameiVAMP2_HUMAN
    AccessioniPrimary (citable) accession number: P63027
    Secondary accession number(s): P19065, Q9BUC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: February 9, 2010
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3