Reviewed, UniProtKB/Swiss-Prot P63027 (VAMP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Vesicle-associated membrane protein 2
      Short name=VAMP-2
Alternative name(s):
    Synaptobrevin-2

Gene names

Name:	VAMP2
Synonyms:	SYB2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subunit structure	Interacts with VAPA and VAPB.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction › synapse › synaptosome. Note: Neuronal synaptic vesicles.
Tissue specificity	Nervous system and skeletal muscle. Ref.7
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	membrane organization Inferred from Experiment. Source: Reactome post-Golgi vesicle-mediated transport Inferred from Experiment. Source: Reactome
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW clathrin sculpted glutamate transport vesicle membrane Inferred from Experiment. Source: Reactome integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc secretory granule membrane Inferred from Experiment. Source: Reactome synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRKD3	O94806	2	EBI-520113,EBI-1255366

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 116

115

Vesicle-associated membrane protein 2

PRO_0000206723

Regions

Topological domain

2 – 94

93

Cytoplasmic Potential

Transmembrane

95 – 114

20

Anchor for type IV membrane protein Potential

Topological domain

115 – 116

2

Vesicular Potential

Domain

31 – 91

61

v-SNARE coiled-coil homology

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

75

1

Phosphoserine By similarity

Experimental info

Sequence conflict

116

1

T → S in AAA60604. Ref.1

Sequence conflict

116

1

T → S in CAA12385. Ref.2

Sequence conflict

116

1

T → S in AAF15551. Ref.3

Secondary structure

1

.

.

.

.

.

.

116

Helix Strand Turn

Details...

Beta strand

34 – 36

3

Turn

47 – 49

3

Helix

50 – 53

4

Sequences

	Sequence		Length	Mass (Da)
	P63027-1 [UniParc]. Last modified February 9, 2010. Version 3. Checksum: 9CD679C4F6F1B5A8	FASTA	116	12,663
	10 20 30 40 50 60 MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL 70 80 90 100 110 SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2." Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C. J. Biol. Chem. 265:17267-17273(1990) [PubMed: 1976629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Expression of VAMP genes in human neutrophils." Nabokina S.M., Lazo P.A., Mollinedo F. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood.
[3]	"Genomic structure of human SYB2 gene." Taruscio D., Zoraqi K.G., Falbo V. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neuroblastoma and Testis.
[7]	"Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H., Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J., Ward C.W. Biochem. J. 317:945-954(1996) [PubMed: 8760387] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane." Kutay U., Ahnert-Hilger G., Hartmann E., Wiedenmann B., Rapoport T.A. EMBO J. 14:217-223(1995) [PubMed: 7835332] [Abstract] Cited for: TOPOLOGY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution." Hanson M.A., Stevens R.C. Nat. Struct. Biol. 7:687-692(2000) [PubMed: 10932255] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 34-89 IN COMPLEX WITH BOTB.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36205 , M36201, M36202, M36203, M36204 Genomic DNA. Translation: AAA60604.1.
AJ225044 mRNA. Translation: CAA12385.1.
AF135372 Genomic DNA. Translation: AAF15551.1.
AK289555 mRNA. Translation: BAF82244.1.
CH471108 Genomic DNA. Translation: EAW90087.1.
BC002737 mRNA. Translation: AAH02737.3.
BC019608 mRNA. Translation: AAH19608.1.
BC033870 mRNA. Translation: AAH33870.1.

IPI

IPI00553138.

PIR

B38315.

UniGene

Hs.25348

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FIE	X-ray	2.10	C/D	22-57	[»]
3FII	X-ray	2.17	B	27-57	[»]

SMR

P63027. Positions 25-93.

DisProt

DP00069.

ModBase

Search...

Protein-protein interaction databases

IntAct

P63027. 2 interactions.

STRING

P63027.

Protein family/group databases

TCDB

1.F.1.1.1. synaptosomal vesicle fusion pore (SVF-Pore) family.

PTM databases

PhosphoSite

P63027.

Proteomic databases

PRIDE

P63027.

Genome annotation databases

Ensembl

ENST00000316509; ENSP00000314214; ENSG00000220205; Homo sapiens. [Genome view]

Organism-specific databases

GeneCards

GC17M007987.

H-InvDB

HIX0020382.

HGNC

HGNC:12643. VAMP2.

MIM

185881. gene.

PharmGKB

PA37267.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21021.

HOVERGEN

P63027.

PhylomeDB

P63027.

Enzyme and pathway databases

Pathway_Interaction_DB

botulinumtoxinpathway. Effects of Botulinum toxin.
insulin_glucose_pathway. Insulin-mediated glucose transport.

Reactome

REACT_11123. Membrane Trafficking.
REACT_11184. Botulinum neurotoxicity.
REACT_13685. Synaptic Transmission.
REACT_1505. Integration of energy metabolism.
REACT_15380. Diabetes pathways.

Gene expression databases

ArrayExpress

P63027.

Bgee

P63027.

CleanEx

HS_VAMP2.

Genevestigator

P63027.

GermOnline

ENSG00000179036. Homo sapiens.

Family and domain databases

InterPro

IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin_met/fun.
[Graphical view]

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PIRSF

PIRSF005409. Synaptobrevin_euk. 1 hit.

PRINTS

PR00219. SYNAPTOBREVN.

PROSITE

PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00042. Botulinum Toxin Type B.

SOURCE

Search...

Entry information

Entry name

VAMP2_HUMAN

Accession

Primary (citable) accession number: P63027
Secondary accession number(s): P19065, Q9BUC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	February 9, 2010
Last modified:	February 9, 2010
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.