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Protein

Vesicle-associated membrane protein 2

Gene

VAMP2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • syntaxin binding Source: AgBase

GO - Biological processi

  • cytochrome complex assembly Source: AgBase
  • exocytosis Source: GO_Central
  • membrane raft distribution Source: AgBase
  • regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • signal transduction Source: AgBase
  • vasodilation Source: AgBase
  • vesicle fusion Source: AgBase
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 2
Short name:
VAMP-2
Alternative name(s):
Synaptobrevin-2
Gene namesi
Name:VAMP2
Synonyms:SYB2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9493CytoplasmicSequence analysisAdd
BLAST
Transmembranei95 – 11420Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini115 – 1162VesicularSequence analysis

GO - Cellular componenti

  • caveola Source: AgBase
  • cell junction Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: AgBase
  • neuron projection Source: UniProtKB-SubCell
  • SNARE complex Source: UniProtKB
  • synaptic vesicle membrane Source: UniProtKB-SubCell
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 116115Vesicle-associated membrane protein 2PRO_0000206722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Post-translational modificationi

Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP63026.
PRIDEiP63026.

Interactioni

Subunit structurei

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with BVES and STX4. Interacts (via N-terminus) with KCNB1 (via N-terminus and C-terminus); stimulates the channel inactivation rate of KCNB1. Interacts with VAPA and VAPB. Interacts with WDFY2, PRKCZ and PRKCI. Forms a complex with WDFY2 and PRKCZ.By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • syntaxin binding Source: AgBase

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005078.

Structurei

3D structure databases

ProteinModelPortaliP63026.
SMRiP63026. Positions 30-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 9161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63026.
KOiK13504.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV
60 70 80 90 100
DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG
110
VICAIILIII IVYFSS
Length:116
Mass (Da):12,649
Last modified:January 23, 2007 - v2
Checksum:i98C679C4F6F1B5A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76199 mRNA. Translation: CAA53792.1.
PIRiJN0011.
RefSeqiNP_776908.1. NM_174483.2.
UniGeneiBt.88332.

Genome annotation databases

GeneIDi282116.
KEGGibta:282116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76199 mRNA. Translation: CAA53792.1.
PIRiJN0011.
RefSeqiNP_776908.1. NM_174483.2.
UniGeneiBt.88332.

3D structure databases

ProteinModelPortaliP63026.
SMRiP63026. Positions 30-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005078.

Proteomic databases

PaxDbiP63026.
PRIDEiP63026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282116.
KEGGibta:282116.

Organism-specific databases

CTDi6844.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP63026.
KOiK13504.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A synaptic vesicle membrane protein is conserved from mammals to Drosophila."
    Suedhof T.C., Baumert M., Perin M.S., Jahn R.
    Neuron 2:1475-1481(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: PROTEIN SEQUENCE OF 32-60 AND 68-83, ACETYLATION AT SER-2.
  3. "A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals."
    Horikawa H.P., Saisu H., Ishizuka T., Sekine Y., Tsugita A., Odani S., Abe T.
    FEBS Lett. 330:236-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 48-79.
    Tissue: Brain.

Entry informationi

Entry nameiVAMP2_BOVIN
AccessioniPrimary (citable) accession number: P63026
Secondary accession number(s): P19065, Q9TRF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.