Reviewed, UniProtKB/Swiss-Prot P63026 (VAMP2_BOVIN)

Names and origin

Protein names

Recommended name:
    Vesicle-associated membrane protein 2
      Short name=VAMP-2
Alternative name(s):
    Synaptobrevin-2

Gene names

Name:	VAMP2
Synonyms:	SYB2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subunit structure	Interacts with VAPA and VAPB.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction › synapse › synaptosome. Note: Neuronal synaptic vesicles.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 116	115	Vesicle-associated membrane protein 2		PRO_0000206722
Regions
	Topological domain	2 – 94	93	Cytoplasmic Potential
	Transmembrane	95 – 114	20	Anchor for type IV membrane protein Potential
	Topological domain	115 – 116	2	Vesicular Potential
	Domain	31 – 91	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.2
	Modified residue	75	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63026-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 98C679C4F6F1B5A8	FASTA	116	12,649
	10 20 30 40 50 60 MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL 70 80 90 100 110 SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG VICAIILIII IVYFSS

References

[1]	"A synaptic vesicle membrane protein is conserved from mammals to Drosophila." Suedhof T.C., Baumert M., Perin M.S., Jahn R. Neuron 2:1475-1481(1989) [PubMed: 2560644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"SNAP receptors implicated in vesicle targeting and fusion." Soellner T., Whiteheart S.W., Brunner M., Erdjument-Bromage H., Geromanos S., Tempst P., Rothman J.E. Nature 362:318-324(1993) [PubMed: 8455717] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-60 AND 68-83, ACETYLATION AT SER-2.
[3]	"A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals." Horikawa H.P., Saisu H., Ishizuka T., Sekine Y., Tsugita A., Odani S., Abe T. FEBS Lett. 330:236-240(1993) [PubMed: 8365494] [Abstract] Cited for: PROTEIN SEQUENCE OF 48-79. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76199 mRNA. Translation: CAA53792.1.
IPI	IPI00696116.
PIR	JN0011.
RefSeq	NP_776908.1.
UniGene	Bt.88332
3D structure databases
SMR	P63026. Positions 25-93.
ModBase	Search...
Protein-protein interaction databases
STRING	P63026.
Genome annotation databases
Ensembl	ENSBTAT00000005078; ENSBTAP00000005078; ENSBTAG00000003891; Bos taurus. [Genome view]
GeneID	282116.
KEGG	bta:282116.
Organism-specific databases
CTD	282116.
Phylogenomic databases
HOVERGEN	P63026.
InParanoid	P63026.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VAMP2_BOVIN

Accession

Primary (citable) accession number: P63026
Secondary accession number(s): P19065, Q9TRF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)